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P31382

- PMT2_YEAST

UniProt

P31382 - PMT2_YEAST

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Protein
Dolichyl-phosphate-mannose--protein mannosyltransferase 2
Gene
PMT2, YAL023C, FUN25
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Transfers mannose from Dol-P-mannose to Ser or Thr residues on proteins. Seems to be active on chitinase.

Catalytic activityi

Dolichyl phosphate D-mannose + protein = dolichyl phosphate + O-D-mannosylprotein.

Pathwayi

GO - Molecular functioni

  1. dolichyl-phosphate-mannose-protein mannosyltransferase activity Source: SGD
  2. protein binding Source: IntAct
Complete GO annotation...

GO - Biological processi

  1. ER-associated misfolded protein catabolic process Source: SGD
  2. protein O-linked glycosylation Source: SGD
  3. protein O-linked mannosylation Source: SGD
  4. protein exit from endoplasmic reticulum Source: SGD
  5. regulation of endoplasmic reticulum unfolded protein response Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Enzyme and pathway databases

BioCyciYEAST:YAL023C-MONOMER.
BRENDAi2.4.1.109. 984.
UniPathwayiUPA00378.

Protein family/group databases

CAZyiGT39. Glycosyltransferase Family 39.

Names & Taxonomyi

Protein namesi
Recommended name:
Dolichyl-phosphate-mannose--protein mannosyltransferase 2 (EC:2.4.1.109)
Gene namesi
Name:PMT2
Ordered Locus Names:YAL023C
ORF Names:FUN25
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL023c.
SGDiS000000021. PMT2.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini2 – 6463Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei65 – 8521Helical; Reviewed prediction
Add
BLAST
Topological domaini86 – 16782Lumenal Reviewed prediction
Add
BLAST
Transmembranei168 – 18821Helical; Reviewed prediction
Add
BLAST
Topological domaini189 – 19810Cytoplasmic Reviewed prediction
Transmembranei199 – 21921Helical; Reviewed prediction
Add
BLAST
Topological domaini220 – 25132Lumenal Reviewed prediction
Add
BLAST
Transmembranei252 – 27221Helical; Reviewed prediction
Add
BLAST
Topological domaini273 – 28917Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei290 – 31021Helical; Reviewed prediction
Add
BLAST
Topological domaini311 – 609299Lumenal Reviewed prediction
Add
BLAST
Transmembranei610 – 63021Helical; Reviewed prediction
Add
BLAST
Topological domaini631 – 64717Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei648 – 66821Helical; Reviewed prediction
Add
BLAST
Topological domaini669 – 6768Lumenal Reviewed prediction
Transmembranei677 – 69721Helical; Reviewed prediction
Add
BLAST
Topological domaini698 – 71013Cytoplasmic Reviewed prediction
Add
BLAST
Transmembranei711 – 73121Helical; Reviewed prediction
Add
BLAST
Topological domaini732 – 75928Lumenal Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt1p-Pmt2p dimer complex Source: SGD
  2. dolichyl-phosphate-mannose-protein mannosyltransferase Pmt5p-Pmt2p dimer complex Source: SGD
  3. integral component of membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed1 Publication
Chaini2 – 759758Dolichyl-phosphate-mannose--protein mannosyltransferase 2
PRO_0000121492Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine1 Publication
Modified residuei32 – 321Phosphoserine1 Publication
Modified residuei38 – 381Phosphoserine1 Publication
Modified residuei39 – 391Phosphoserine1 Publication
Glycosylationi131 – 1311N-linked (GlcNAc...) Reviewed prediction
Glycosylationi155 – 1551N-linked (GlcNAc...) Reviewed prediction
Glycosylationi403 – 4031N-linked (GlcNAc...) Reviewed prediction

Keywords - PTMi

Acetylation, Glycoprotein, Phosphoprotein

Proteomic databases

MaxQBiP31382.
PaxDbiP31382.
PeptideAtlasiP31382.

Expressioni

Gene expression databases

GenevestigatoriP31382.

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
PMT1P337754EBI-13573,EBI-13567
PMT5P528672EBI-13573,EBI-13591

Protein-protein interaction databases

BioGridi31743. 170 interactions.
DIPiDIP-6720N.
IntActiP31382. 3 interactions.
MINTiMINT-655858.
STRINGi4932.YAL023C.

Structurei

3D structure databases

ProteinModelPortaliP31382.
SMRiP31382. Positions 358-510.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini339 – 39456MIR 1
Add
BLAST
Domaini407 – 46357MIR 2
Add
BLAST
Domaini471 – 52959MIR 3
Add
BLAST

Sequence similaritiesi

Contains 3 MIR domains.

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG1928.
GeneTreeiENSGT00740000115531.
HOGENOMiHOG000157526.
KOiK00728.
OMAiWLCISRF.
OrthoDBiEOG7BP89X.

Family and domain databases

InterProiIPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view]
PANTHERiPTHR10050. PTHR10050. 1 hit.
PfamiPF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view]
SMARTiSM00472. MIR. 3 hits.
[Graphical view]
SUPFAMiSSF82109. SSF82109. 1 hit.
PROSITEiPS50919. MIR. 3 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31382-1 [UniParc]FASTAAdd to Basket

« Hide

MSSSSSTGYS KNNAAHIKQE NTLRQRESSS ISVSEELSSA DERDAEDFSK    50
EKPAAQSSLL RLESVVMPVI FTALALFTRM YKIGINNHVV WDEAHFGKFG 100
SYYLRHEFYH DVHPPLGKML VGLSGYLAGY NGSWDFPSGE IYPDYLDYVK 150
MRLFNASFSA LCVPLAYFTA KAIGFSLPTV WLMTVLVLFE NSYSTLGRFI 200
LLDSMLLFFT VASFFSFVMF HNQRSKPFSR KWWKWLLITG ISLGCTISVK 250
MVGLFIITMV GIYTVIDLWT FLADKSMSWK TYINHWLARI FGLIIVPFCI 300
FLLCFKIHFD LLSHSGTGDA NMPSLFQARL VGSDVGQGPR DIALGSSVVS 350
IKNQALGGSL LHSHIQTYPD GSNQQQVTCY GYKDANNEWF FNRERGLPSW 400
SENETDIEYL KPGTSYRLVH KSTGRNLHTH PVAAPVSKTQ WEVSGYGDNV 450
VGDNKDNWVI EIMDQRGDED PEKLHTLTTS FRIKNLEMGC YLAQTGNSLP 500
EWGFRQQEVV CMKNPFKRDK RTWWNIETHE NERLPPRPED FQYPKTNFLK 550
DFIHLNLAMM ATNNALVPDP DKFDYLASSA WQWPTLNVGL RLCGWGDDNP 600
KYFLLGTPAS TWASSVAVLA FMATVVILLI RWQRQYVDLR NPSNWNVFLM 650
GGFYPLLAWG LHYMPFVIMS RVTYVHHYLP ALYFALIILA YCFDAGLQKW 700
SRSKCGRIMR FVLYAGFMAL VIGCFWYFSP ISFGMEGPSS NFRYLNWFST 750
WDIADKQEA 759
Length:759
Mass (Da):86,870
Last modified:June 16, 2003 - v2
Checksum:iB1E1480C2E04BE3D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05146 Genomic DNA. Translation: AAC04934.2.
BK006935 Genomic DNA. Translation: DAA06965.1.
PIRiS36711.
RefSeqiNP_009379.2. NM_001178168.1.

Genome annotation databases

EnsemblFungiiYAL023C; YAL023C; YAL023C.
GeneIDi851210.
KEGGisce:YAL023C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05146 Genomic DNA. Translation: AAC04934.2 .
BK006935 Genomic DNA. Translation: DAA06965.1 .
PIRi S36711.
RefSeqi NP_009379.2. NM_001178168.1.

3D structure databases

ProteinModelPortali P31382.
SMRi P31382. Positions 358-510.
ModBasei Search...

Protein-protein interaction databases

BioGridi 31743. 170 interactions.
DIPi DIP-6720N.
IntActi P31382. 3 interactions.
MINTi MINT-655858.
STRINGi 4932.YAL023C.

Protein family/group databases

CAZyi GT39. Glycosyltransferase Family 39.

Proteomic databases

MaxQBi P31382.
PaxDbi P31382.
PeptideAtlasi P31382.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL023C ; YAL023C ; YAL023C .
GeneIDi 851210.
KEGGi sce:YAL023C.

Organism-specific databases

CYGDi YAL023c.
SGDi S000000021. PMT2.

Phylogenomic databases

eggNOGi COG1928.
GeneTreei ENSGT00740000115531.
HOGENOMi HOG000157526.
KOi K00728.
OMAi WLCISRF.
OrthoDBi EOG7BP89X.

Enzyme and pathway databases

UniPathwayi UPA00378 .
BioCyci YEAST:YAL023C-MONOMER.
BRENDAi 2.4.1.109. 984.

Miscellaneous databases

NextBioi 968085.

Gene expression databases

Genevestigatori P31382.

Family and domain databases

InterProi IPR027005. GlyclTrfase_39_like.
IPR003342. Glyco_trans_39.
IPR016093. MIR_motif.
[Graphical view ]
PANTHERi PTHR10050. PTHR10050. 1 hit.
Pfami PF02815. MIR. 1 hit.
PF02366. PMT. 1 hit.
[Graphical view ]
SMARTi SM00472. MIR. 3 hits.
[Graphical view ]
SUPFAMi SSF82109. SSF82109. 1 hit.
PROSITEi PS50919. MIR. 3 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
    Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
    Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  3. Dolinski K.J., Cherry J.M.
    Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: SEQUENCE REVISION TO 137-139.
  4. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  5. "Protein O-glycosylation in yeast. The PMT2 gene specifies a second protein O-mannosyltransferase that functions in addition to the PMT1-encoded activity."
    Lussier M., Gentzsch M., Sdicu A.-M., Bussey H., Tanner W.
    J. Biol. Chem. 270:2770-2775(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  6. "The PMT gene family: protein O-glycosylation in Saccharomyces cerevisiae is vital."
    Gaentzsch M., Tanner W.
    EMBO J. 15:5752-5759(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
  7. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  8. "A global topology map of the Saccharomyces cerevisiae membrane proteome."
    Kim H., Melen K., Oesterberg M., von Heijne G.
    Proc. Natl. Acad. Sci. U.S.A. 103:11142-11147(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: ATCC 208353 / W303-1A.
  9. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  10. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-32; SER-38 AND SER-39, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT SER-2, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS], CLEAVAGE OF INITIATOR METHIONINE [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPMT2_YEAST
AccessioniPrimary (citable) accession number: P31382
Secondary accession number(s): D6VPJ5
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: June 16, 2003
Last modified: September 3, 2014
This is version 141 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6510 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

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