P31381 (FUN26_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Nucleoside transporter FUN26

Gene names

Name:	FUN26
Ordered Locus Names:	YAL022C

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	517 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Has broad nucleoside selectivity (uridine, ademosine and cytidine) and most likely functions to transport nucleosides across intracellular membranes.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Miscellaneous	Present with 217 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SLC29A/ENT transporter (TC 2.A.57) family. [View classification]

Ontologies

Keywords
Biological process	Transport
Cellular component	Membrane
Domain	Transmembrane Transmembrane helix
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Cellular_component	fungal-type vacuole membrane Inferred from direct assay PubMed 21349851. Source: SGD integral to membrane Inferred from sequence model PubMed 12192589. Source: SGD
Molecular_function	nicotinamide riboside transmembrane transporter activity Inferred from mutant phenotype PubMed 21349851. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 517	517	Nucleoside transporter FUN26		PRO_0000209346
Regions
	Transmembrane	76 – 96	21	Helical; Potential
	Transmembrane	116 – 136	21	Helical; Potential
	Transmembrane	151 – 171	21	Helical; Potential
	Transmembrane	174 – 194	21	Helical; Potential
	Transmembrane	214 – 234	21	Helical; Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Transmembrane	344 – 364	21	Helical; Potential
	Transmembrane	367 – 387	21	Helical; Potential
	Transmembrane	411 – 431	21	Helical; Potential
	Transmembrane	446 – 466	21	Helical; Potential
	Transmembrane	492 – 512	21	Helical; Potential
Amino acid modifications
	Modified residue	27	1	Phosphoserine Ref.6

Sequences

	Sequence		Length	Mass (Da)
	P31381 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 43C92A3E9A3D8D50	FASTA	517	58,317
	10 20 30 40 50 60 MSTSADTDTI KKPILAVPEP ALADTHSEEI SRSGEEHESE NNEHSDEEGD NYSEREQSVS 70 80 90 100 110 120 TEPLDTLPLR KKLKNLSYIT FFAIGIGLLW PWNCILSASQ YFKHDIFKDT SIWAKIFTSS 130 140 150 160 170 180 MMSFSTISSM LFNIYLAKRQ YKYSRRVING LVWEIIVFTV MCFFTILHFL LPKWFNFMFI 190 200 210 220 230 240 MMLVVISSMG TAMTQNGIMA IANVFGSEYS QGVMVGQAVA GVLPSLVLFA LAFIENSSVS 250 260 270 280 290 300 TTGGILLYFF TTTLVVTICV VMFSVSKISR KVNENWNVED GHITDVLLGS LRSNEEEIRI 310 320 330 340 350 360 VGRIDQMEDE DHRRTNGTRD DNDEGEELQL KVPFEVLFAK LKYLVLSIFT TFVVTLVFPV 370 380 390 400 410 420 FASATYVTGL PLSNAQYIPL IFTLWNLGDL YGRVIADWPM FRDQKFTPRK TFIYSLLRVA 430 440 450 460 470 480 AIPLFLMFTA ITSSSSGDEE HNGSVIVDLC YMLLQFLFGV TNGHVISMSF MKVPEQLDND 490 500 510 DEKEAAGGFT NIFVSTGLAL GSIISYVFVF IIDFIIR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes." Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H. Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Nucleoside transporter proteins of Saccharomyces cerevisiae. Demonstration of a transporter (FUI1) with high uridine selectivity in plasma membranes and a transporter (FUN26) with broad nucleoside selectivity in intracellular membranes." Vickers M.F., Yao S.Y., Baldwin S.A., Young J.D., Cass C.E. J. Biol. Chem. 275:25931-25938(2000) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION.
[5]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[6]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-27, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L05146 Genomic DNA. Translation: AAC04935.1. BK006935 Genomic DNA. Translation: DAA06966.1.
PIR	S36712.
RefSeq	NP_009380.1. NM_001178167.1.
3D structure databases
ProteinModelPortal	P31381.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5495N.
IntAct	P31381. 1 interaction.
MINT	MINT-518779.
STRING	4932.YAL022C.
Protein family/group databases
TCDB	2.A.57.3.1. equilibrative nucleoside transporter (ENT) family.
Proteomic databases
PaxDb	P31381.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL022C; YAL022C; YAL022C.
GeneID	851211.
KEGG	sce:YAL022C.
Organism-specific databases
CYGD	YAL022c.
SGD	S000000020. FUN26.
Phylogenomic databases
eggNOG	NOG299983.
GeneTree	ENSGT00390000002232.
HOGENOM	HOG000190304.
KO	K15014.
OMA	HESENNE.
OrthoDB	EOG4C8C2B.
Gene expression databases
Genevestigator	P31381.
GermOnline	YAL022C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR002259. Eqnu_transpt. IPR016196. MFS_dom_general_subst_transpt. [Graphical view]
PANTHER	PTHR10332. PTHR10332. 1 hit.
Pfam	PF01733. Nucleoside_tran. 1 hit. [Graphical view]
PIRSF	PIRSF016379. ENT. 1 hit.
PRINTS	PR01130. DERENTRNSPRT.
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
TIGRFAMs	TIGR00939. 2a57. 1 hit.
ProtoNet	Search...
Other
NextBio	968088.

Entry information

Entry name

FUN26_YEAST

Accession

Primary (citable) accession number: P31381
Secondary accession number(s): D6VPJ6

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program