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P31380

- FUN30_YEAST

UniProt

P31380 - FUN30_YEAST

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Protein

ATP-dependent helicase FUN30

Gene

FUN30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs, facilitating single-stranded DNA (ssDNA) production by the EXO1 and SGS1 machinery. Promotes gene silencing at heterochromatin by regulating the chromatin structure within or around silent loci. Also required for heterochromatin organization at centromeres.6 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi597 – 6048ATPCurated

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. chromatin binding Source: SGD
  3. DNA binding Source: SGD
  4. DNA-dependent ATPase activity Source: SGD
  5. helicase activity Source: UniProtKB-KW

GO - Biological processi

  1. ATP catabolic process Source: GOC
  2. ATP-dependent chromatin remodeling Source: SGD
  3. chromatin silencing at rDNA Source: SGD
  4. chromatin silencing at silent mating-type cassette Source: SGD
  5. chromatin silencing at telomere Source: SGD
  6. DNA double-strand break processing Source: SGD
  7. heterochromatin assembly involved in chromatin silencing Source: SGD
  8. heterochromatin maintenance involved in chromatin silencing Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28831-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase FUN30 (EC:3.6.4.12)
Gene namesi
Name:FUN30
Ordered Locus Names:YAL019W
ORF Names:YAL001
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL019w.
SGDiS000000017. FUN30.

Subcellular locationi

Nucleus. Chromosome
Note: Recruited to double-strand breaks (DSBs) sites of DNA damage.

GO - Cellular componenti

  1. chromosome, centromeric region Source: SGD
  2. mating-type region heterochromatin Source: SGD
  3. nucleus Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired ability to repair double-strand breaks (DSBs).1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi603 – 6031K → R: Abolishes both the silencing function and the ability to promote DNA end resection of double-strand breaks (DSBs). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11311131ATP-dependent helicase FUN30PRO_0000074384Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei232 – 2321Phosphoserine1 Publication
Modified residuei369 – 3691Phosphoserine1 Publication
Modified residuei451 – 4511Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31380.
PaxDbiP31380.
PeptideAtlasiP31380.

Expressioni

Gene expression databases

GenevestigatoriP31380.

Interactioni

Subunit structurei

Homodimer.1 Publication

Protein-protein interaction databases

BioGridi31747. 89 interactions.
DIPiDIP-2541N.
IntActiP31380. 10 interactions.
MINTiMINT-425278.
STRINGi4932.YAL019W.

Structurei

3D structure databases

ProteinModelPortaliP31380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini584 – 752169Helicase ATP-bindingPROSITE-ProRule annotationAdd
BLAST
Domaini953 – 1108156Helicase C-terminalPROSITE-ProRule annotationAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni76 – 11136CUE-like regionAdd
BLAST

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi703 – 7064DEGH box

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG0553.
GeneTreeiENSGT00630000089890.
HOGENOMiHOG000195581.
InParanoidiP31380.
KOiK14439.
OMAiREFNKFC.
OrthoDBiEOG7WDNB3.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31380-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSGSHSNDED DVVQVPETSS PTKVASSSPL KPTSPTVPDA SVASLRSRFT
60 70 80 90 100
FKPSDPSEGA HTSKPLPSGS PEVALVNLAR EFPDFSQTLV QAVFKSNSFN
110 120 130 140 150
LQSARERLTR LRQQRQNWTW NKNASPKKSE TPPPVKKSLP LANTGRLSSI
160 170 180 190 200
HGNINNKSSK ITVAKQKTSI FDRYSNVINQ KQYTFELPTN LNIDSEALSK
210 220 230 240 250
LPVNYNKKRR LVRADQHPIG KSYESSATQL GSAREKLLAN RKYGRHANDN
260 270 280 290 300
DEEEEESMMT DDDDASGDDY TESTPQINLD EQVLQFINDS DIVDLSDLSD
310 320 330 340 350
TTMHKAQLIA SHRPYSSLNA FVNTNFNDKD TEENASNKRK RRAAASANES
360 370 380 390 400
ERLLDKITQS IRGYNAIESV IKKCSSYGDL VTSQMKKWGV QVEGDNSELD
410 420 430 440 450
LMNLGEDDDD DNDDGNNDNN NSNNNNTAGA DATSKEKEDT KAVVEGFDET
460 470 480 490 500
SAEPTPAPAP APVERETKRI RNTTKPKVVE DEDDDVDLEA IDDELPQSEH
510 520 530 540 550
EDDDYEEEDE DYNDEEEDVE YDDGDDDDDD DDEFVATRKN THVISTTSRN
560 570 580 590 600
GRKPIVKFFK GKPRLLSPEI SLKDYQQTGI NWLNLLYQNK MSCILADDMG
610 620 630 640 650
LGKTCQVISF FAYLKQINEP GPHLVVVPSS TLENWLREFQ KFAPALKIEP
660 670 680 690 700
YYGSLQEREE LRDILERNAG KYDVIVTTYN LAAGNKYDVS FLKNRNFNVV
710 720 730 740 750
VYDEGHMLKN STSERFAKLM KIRANFRLLL TGTPLQNNLK ELMSLLEFIM
760 770 780 790 800
PNLFISKKES FDAIFKQRAK TTDDNKNHNP LLAQEAITRA KTMMKPFILR
810 820 830 840 850
RRKDQVLKHL PPKHTHIQYC ELNAIQKKIY DKEIQIVLEH KRMIKDGELP
860 870 880 890 900
KDAKEKSKLQ SSSSKNLIMA LRKASLHPLL FRNIYNDKII TKMSDAILDE
910 920 930 940 950
PAYAENGNKE YIKEDMSYMT DFELHKLCCN FPNTLSKYQL HNDEWMQSGK
960 970 980 990 1000
IDALKKLLKT IIVDKQEKVL IFSLFTQVLD ILEMVLSTLD YKFLRLDGST
1010 1020 1030 1040 1050
QVNDRQLLID KFYEDKDIPI FILSTKAGGF GINLVCANNV IIFDQSFNPH
1060 1070 1080 1090 1100
DDRQAADRAH RVGQTKEVNI TTLITKDSIE EKIHQLAKNK LALDSYISED
1110 1120 1130
KKSQDVLESK VSDMLEDIIY DENSKPKGTK E
Length:1,131
Mass (Da):128,507
Last modified:July 1, 1993 - v1
Checksum:i7E61B9ABB3A42ED2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04938.1.
BK006935 Genomic DNA. Translation: DAA06969.1.
PIRiS22266.
RefSeqiNP_009383.1. NM_001178164.1.

Genome annotation databases

EnsemblFungiiYAL019W; YAL019W; YAL019W.
GeneIDi851214.
KEGGisce:YAL019W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04938.1 .
BK006935 Genomic DNA. Translation: DAA06969.1 .
PIRi S22266.
RefSeqi NP_009383.1. NM_001178164.1.

3D structure databases

ProteinModelPortali P31380.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31747. 89 interactions.
DIPi DIP-2541N.
IntActi P31380. 10 interactions.
MINTi MINT-425278.
STRINGi 4932.YAL019W.

Proteomic databases

MaxQBi P31380.
PaxDbi P31380.
PeptideAtlasi P31380.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL019W ; YAL019W ; YAL019W .
GeneIDi 851214.
KEGGi sce:YAL019W.

Organism-specific databases

CYGDi YAL019w.
SGDi S000000017. FUN30.

Phylogenomic databases

eggNOGi COG0553.
GeneTreei ENSGT00630000089890.
HOGENOMi HOG000195581.
InParanoidi P31380.
KOi K14439.
OMAi REFNKFC.
OrthoDBi EOG7WDNB3.

Enzyme and pathway databases

BioCyci YEAST:G3O-28831-MONOMER.

Miscellaneous databases

NextBioi 968097.

Gene expression databases

Genevestigatori P31380.

Family and domain databases

Gene3Di 3.40.50.300. 2 hits.
InterProi IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view ]
Pfami PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view ]
SMARTi SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view ]
SUPFAMi SSF52540. SSF52540. 3 hits.
PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I."
    Clark M.W., Zhong W.-W., Keng T., Storms R.K., Barton A.B., Kaback D.B., Bussey H.
    Yeast 8:133-145(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
    Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
    Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region."
    Barton A.B., Kaback D.B.
    J. Bacteriol. 176:1872-1880(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION.
    Strain: ATCC 204508 / S288c.
  6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-369, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. "The SNF2-family member Fun30 promotes gene silencing in heterochromatic loci."
    Neves-Costa A., Will W.R., Vetter A.T., Miller J.R., Varga-Weisz P.
    PLoS ONE 4:E8111-E8111(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  11. "The Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme."
    Awad S., Ryan D., Prochasson P., Owen-Hughes T., Hassan A.H.
    J. Biol. Chem. 285:9477-9484(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBUNIT.
  12. "The Saccharomyces cerevisiae chromatin remodeler Fun30 regulates DNA end-resection and checkpoint deactivation."
    Eapen V.V., Sugawara N., Tsabar M., Wu W.H., Haber J.E.
    Mol. Cell. Biol. 32:4727-4740(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  13. "The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends."
    Chen X., Cui D., Papusha A., Zhang X., Chu C.D., Tang J., Chen K., Pan X., Ira G.
    Nature 489:576-580(2012) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
  14. Cited for: FUNCTION, MUTAGENESIS OF LYS-603.
  15. Cited for: FUNCTION.

Entry informationi

Entry nameiFUN30_YEAST
AccessioniPrimary (citable) accession number: P31380
Secondary accession number(s): D6VPJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 128 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.1 Publication

Caution

Was initially reported to contain a CUE domain (PubMed:19956593). However, no CUE domain is predicted by prediction tools and the CUE-like region does not show no affinity for ubiquitinated histones (PubMed:20075079).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3