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Protein

ATP-dependent helicase FUN30

Gene

FUN30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs, facilitating single-stranded DNA (ssDNA) production by the EXO1 and SGS1 machinery. Promotes gene silencing at heterochromatin by regulating the chromatin structure within or around silent loci. Also required for heterochromatin organization at centromeres.6 Publications

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi597 – 604ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: SGD
  • DNA binding Source: SGD
  • DNA-dependent ATPase activity Source: SGD
  • helicase activity Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: SGD
  • chromatin modification Source: UniProtKB-KW
  • chromatin remodeling Source: SGD
  • chromatin silencing at rDNA Source: SGD
  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at telomere Source: SGD
  • DNA double-strand break processing Source: SGD
  • heterochromatin assembly involved in chromatin silencing Source: SGD
  • heterochromatin maintenance involved in chromatin silencing Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • nucleosome mobilization Source: SGD
  • nucleosome positioning Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Chromatin regulator, Helicase, Hydrolase

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

ATP-binding, DNA-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28831-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase FUN30 (EC:3.6.4.12)
Gene namesi
Name:FUN30
Ordered Locus Names:YAL019W
ORF Names:YAL001
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL019W.
SGDiS000000017. FUN30.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: SGD
  • chromosome, telomeric region Source: GOC
  • mating-type region heterochromatin Source: SGD
  • nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired ability to repair double-strand breaks (DSBs).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi603K → R: Abolishes both the silencing function and the ability to promote DNA end resection of double-strand breaks (DSBs). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743841 – 1131ATP-dependent helicase FUN30Add BLAST1131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei232PhosphoserineCombined sources1
Modified residuei369PhosphoserineCombined sources1
Modified residuei451PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31380.
PRIDEiP31380.

PTM databases

iPTMnetiP31380.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-20621,EBI-20621

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi31747. 94 interactors.
DIPiDIP-2541N.
IntActiP31380. 10 interactors.
MINTiMINT-425278.

Structurei

3D structure databases

ProteinModelPortaliP31380.
SMRiP31380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini584 – 752Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini953 – 1108Helicase C-terminalPROSITE-ProRule annotationAdd BLAST156

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 111CUE-like regionAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi703 – 706DEGH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated
Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

Phylogenomic databases

GeneTreeiENSGT00860000133865.
HOGENOMiHOG000195581.
InParanoidiP31380.
KOiK14439.
OMAiNVIIFDQ.
OrthoDBiEOG092C0RAA.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSHSNDED DVVQVPETSS PTKVASSSPL KPTSPTVPDA SVASLRSRFT
60 70 80 90 100
FKPSDPSEGA HTSKPLPSGS PEVALVNLAR EFPDFSQTLV QAVFKSNSFN
110 120 130 140 150
LQSARERLTR LRQQRQNWTW NKNASPKKSE TPPPVKKSLP LANTGRLSSI
160 170 180 190 200
HGNINNKSSK ITVAKQKTSI FDRYSNVINQ KQYTFELPTN LNIDSEALSK
210 220 230 240 250
LPVNYNKKRR LVRADQHPIG KSYESSATQL GSAREKLLAN RKYGRHANDN
260 270 280 290 300
DEEEEESMMT DDDDASGDDY TESTPQINLD EQVLQFINDS DIVDLSDLSD
310 320 330 340 350
TTMHKAQLIA SHRPYSSLNA FVNTNFNDKD TEENASNKRK RRAAASANES
360 370 380 390 400
ERLLDKITQS IRGYNAIESV IKKCSSYGDL VTSQMKKWGV QVEGDNSELD
410 420 430 440 450
LMNLGEDDDD DNDDGNNDNN NSNNNNTAGA DATSKEKEDT KAVVEGFDET
460 470 480 490 500
SAEPTPAPAP APVERETKRI RNTTKPKVVE DEDDDVDLEA IDDELPQSEH
510 520 530 540 550
EDDDYEEEDE DYNDEEEDVE YDDGDDDDDD DDEFVATRKN THVISTTSRN
560 570 580 590 600
GRKPIVKFFK GKPRLLSPEI SLKDYQQTGI NWLNLLYQNK MSCILADDMG
610 620 630 640 650
LGKTCQVISF FAYLKQINEP GPHLVVVPSS TLENWLREFQ KFAPALKIEP
660 670 680 690 700
YYGSLQEREE LRDILERNAG KYDVIVTTYN LAAGNKYDVS FLKNRNFNVV
710 720 730 740 750
VYDEGHMLKN STSERFAKLM KIRANFRLLL TGTPLQNNLK ELMSLLEFIM
760 770 780 790 800
PNLFISKKES FDAIFKQRAK TTDDNKNHNP LLAQEAITRA KTMMKPFILR
810 820 830 840 850
RRKDQVLKHL PPKHTHIQYC ELNAIQKKIY DKEIQIVLEH KRMIKDGELP
860 870 880 890 900
KDAKEKSKLQ SSSSKNLIMA LRKASLHPLL FRNIYNDKII TKMSDAILDE
910 920 930 940 950
PAYAENGNKE YIKEDMSYMT DFELHKLCCN FPNTLSKYQL HNDEWMQSGK
960 970 980 990 1000
IDALKKLLKT IIVDKQEKVL IFSLFTQVLD ILEMVLSTLD YKFLRLDGST
1010 1020 1030 1040 1050
QVNDRQLLID KFYEDKDIPI FILSTKAGGF GINLVCANNV IIFDQSFNPH
1060 1070 1080 1090 1100
DDRQAADRAH RVGQTKEVNI TTLITKDSIE EKIHQLAKNK LALDSYISED
1110 1120 1130
KKSQDVLESK VSDMLEDIIY DENSKPKGTK E
Length:1,131
Mass (Da):128,507
Last modified:July 1, 1993 - v1
Checksum:i7E61B9ABB3A42ED2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04938.1.
BK006935 Genomic DNA. Translation: DAA06969.1.
PIRiS22266.
RefSeqiNP_009383.1. NM_001178164.1.

Genome annotation databases

EnsemblFungiiYAL019W; YAL019W; YAL019W.
GeneIDi851214.
KEGGisce:YAL019W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04938.1.
BK006935 Genomic DNA. Translation: DAA06969.1.
PIRiS22266.
RefSeqiNP_009383.1. NM_001178164.1.

3D structure databases

ProteinModelPortaliP31380.
SMRiP31380.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31747. 94 interactors.
DIPiDIP-2541N.
IntActiP31380. 10 interactors.
MINTiMINT-425278.

PTM databases

iPTMnetiP31380.

Proteomic databases

MaxQBiP31380.
PRIDEiP31380.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL019W; YAL019W; YAL019W.
GeneIDi851214.
KEGGisce:YAL019W.

Organism-specific databases

EuPathDBiFungiDB:YAL019W.
SGDiS000000017. FUN30.

Phylogenomic databases

GeneTreeiENSGT00860000133865.
HOGENOMiHOG000195581.
InParanoidiP31380.
KOiK14439.
OMAiNVIIFDQ.
OrthoDBiEOG092C0RAA.

Enzyme and pathway databases

BioCyciYEAST:G3O-28831-MONOMER.

Miscellaneous databases

PROiP31380.

Family and domain databases

Gene3Di3.40.50.300. 2 hits.
InterProiIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamiPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTiSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiFUN30_YEAST
AccessioniPrimary (citable) accession number: P31380
Secondary accession number(s): D6VPJ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 147 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.1 Publication

Caution

Was initially reported to contain a CUE domain (PubMed:19956593). However, no CUE domain is predicted by prediction tools and the CUE-like region does not show no affinity for ubiquitinated histones (PubMed:20075079).2 Publications

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.