Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P31380

- FUN30_YEAST

UniProt

P31380 - FUN30_YEAST

Protein

ATP-dependent helicase FUN30

Gene

FUN30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 127 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs, facilitating single-stranded DNA (ssDNA) production by the EXO1 and SGS1 machinery. Promotes gene silencing at heterochromatin by regulating the chromatin structure within or around silent loci. Also required for heterochromatin organization at centromeres.6 Publications

    Catalytic activityi

    ATP + H2O = ADP + phosphate.

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi597 – 6048ATPCurated

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. chromatin binding Source: SGD
    3. DNA binding Source: SGD
    4. DNA-dependent ATPase activity Source: SGD
    5. helicase activity Source: UniProtKB-KW

    GO - Biological processi

    1. ATP catabolic process Source: GOC
    2. ATP-dependent chromatin remodeling Source: SGD
    3. chromatin silencing at rDNA Source: SGD
    4. chromatin silencing at silent mating-type cassette Source: SGD
    5. chromatin silencing at telomere Source: SGD
    6. DNA double-strand break processing Source: SGD
    7. heterochromatin assembly involved in chromatin silencing Source: SGD
    8. heterochromatin maintenance involved in chromatin silencing Source: SGD

    Keywords - Molecular functioni

    Chromatin regulator, Helicase, Hydrolase

    Keywords - Biological processi

    DNA damage, DNA repair

    Keywords - Ligandi

    ATP-binding, DNA-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciYEAST:G3O-28831-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    ATP-dependent helicase FUN30 (EC:3.6.4.12)
    Gene namesi
    Name:FUN30
    Ordered Locus Names:YAL019W
    ORF Names:YAL001
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    CYGDiYAL019w.
    SGDiS000000017. FUN30.

    Subcellular locationi

    Nucleus. Chromosome
    Note: Recruited to double-strand breaks (DSBs) sites of DNA damage.

    GO - Cellular componenti

    1. chromosome, centromeric region Source: SGD
    2. mating-type region heterochromatin Source: SGD
    3. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chromosome, Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Impaired ability to repair double-strand breaks (DSBs).1 Publication

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi603 – 6031K → R: Abolishes both the silencing function and the ability to promote DNA end resection of double-strand breaks (DSBs). 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11311131ATP-dependent helicase FUN30PRO_0000074384Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei232 – 2321Phosphoserine1 Publication
    Modified residuei369 – 3691Phosphoserine1 Publication
    Modified residuei451 – 4511Phosphoserine1 Publication

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP31380.
    PaxDbiP31380.
    PeptideAtlasiP31380.

    Expressioni

    Gene expression databases

    GenevestigatoriP31380.

    Interactioni

    Subunit structurei

    Homodimer.1 Publication

    Protein-protein interaction databases

    BioGridi31747. 89 interactions.
    DIPiDIP-2541N.
    IntActiP31380. 10 interactions.
    MINTiMINT-425278.
    STRINGi4932.YAL019W.

    Structurei

    3D structure databases

    ProteinModelPortaliP31380.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini584 – 752169Helicase ATP-bindingPROSITE-ProRule annotationAdd
    BLAST
    Domaini953 – 1108156Helicase C-terminalPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni76 – 11136CUE-like regionAdd
    BLAST

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi703 – 7064DEGH box

    Sequence similaritiesi

    Belongs to the SNF2/RAD54 helicase family.Curated
    Contains 1 helicase ATP-binding domain.PROSITE-ProRule annotation
    Contains 1 helicase C-terminal domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0553.
    GeneTreeiENSGT00630000089890.
    HOGENOMiHOG000195581.
    KOiK14439.
    OMAiREFNKFC.
    OrthoDBiEOG7WDNB3.

    Family and domain databases

    Gene3Di3.40.50.300. 2 hits.
    InterProiIPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view]
    PfamiPF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view]
    SMARTiSM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view]
    SUPFAMiSSF52540. SSF52540. 3 hits.
    PROSITEiPS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31380-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSGSHSNDED DVVQVPETSS PTKVASSSPL KPTSPTVPDA SVASLRSRFT     50
    FKPSDPSEGA HTSKPLPSGS PEVALVNLAR EFPDFSQTLV QAVFKSNSFN 100
    LQSARERLTR LRQQRQNWTW NKNASPKKSE TPPPVKKSLP LANTGRLSSI 150
    HGNINNKSSK ITVAKQKTSI FDRYSNVINQ KQYTFELPTN LNIDSEALSK 200
    LPVNYNKKRR LVRADQHPIG KSYESSATQL GSAREKLLAN RKYGRHANDN 250
    DEEEEESMMT DDDDASGDDY TESTPQINLD EQVLQFINDS DIVDLSDLSD 300
    TTMHKAQLIA SHRPYSSLNA FVNTNFNDKD TEENASNKRK RRAAASANES 350
    ERLLDKITQS IRGYNAIESV IKKCSSYGDL VTSQMKKWGV QVEGDNSELD 400
    LMNLGEDDDD DNDDGNNDNN NSNNNNTAGA DATSKEKEDT KAVVEGFDET 450
    SAEPTPAPAP APVERETKRI RNTTKPKVVE DEDDDVDLEA IDDELPQSEH 500
    EDDDYEEEDE DYNDEEEDVE YDDGDDDDDD DDEFVATRKN THVISTTSRN 550
    GRKPIVKFFK GKPRLLSPEI SLKDYQQTGI NWLNLLYQNK MSCILADDMG 600
    LGKTCQVISF FAYLKQINEP GPHLVVVPSS TLENWLREFQ KFAPALKIEP 650
    YYGSLQEREE LRDILERNAG KYDVIVTTYN LAAGNKYDVS FLKNRNFNVV 700
    VYDEGHMLKN STSERFAKLM KIRANFRLLL TGTPLQNNLK ELMSLLEFIM 750
    PNLFISKKES FDAIFKQRAK TTDDNKNHNP LLAQEAITRA KTMMKPFILR 800
    RRKDQVLKHL PPKHTHIQYC ELNAIQKKIY DKEIQIVLEH KRMIKDGELP 850
    KDAKEKSKLQ SSSSKNLIMA LRKASLHPLL FRNIYNDKII TKMSDAILDE 900
    PAYAENGNKE YIKEDMSYMT DFELHKLCCN FPNTLSKYQL HNDEWMQSGK 950
    IDALKKLLKT IIVDKQEKVL IFSLFTQVLD ILEMVLSTLD YKFLRLDGST 1000
    QVNDRQLLID KFYEDKDIPI FILSTKAGGF GINLVCANNV IIFDQSFNPH 1050
    DDRQAADRAH RVGQTKEVNI TTLITKDSIE EKIHQLAKNK LALDSYISED 1100
    KKSQDVLESK VSDMLEDIIY DENSKPKGTK E 1131
    Length:1,131
    Mass (Da):128,507
    Last modified:July 1, 1993 - v1
    Checksum:i7E61B9ABB3A42ED2
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05146 Genomic DNA. Translation: AAC04938.1.
    BK006935 Genomic DNA. Translation: DAA06969.1.
    PIRiS22266.
    RefSeqiNP_009383.1. NM_001178164.1.

    Genome annotation databases

    EnsemblFungiiYAL019W; YAL019W; YAL019W.
    GeneIDi851214.
    KEGGisce:YAL019W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05146 Genomic DNA. Translation: AAC04938.1 .
    BK006935 Genomic DNA. Translation: DAA06969.1 .
    PIRi S22266.
    RefSeqi NP_009383.1. NM_001178164.1.

    3D structure databases

    ProteinModelPortali P31380.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31747. 89 interactions.
    DIPi DIP-2541N.
    IntActi P31380. 10 interactions.
    MINTi MINT-425278.
    STRINGi 4932.YAL019W.

    Proteomic databases

    MaxQBi P31380.
    PaxDbi P31380.
    PeptideAtlasi P31380.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL019W ; YAL019W ; YAL019W .
    GeneIDi 851214.
    KEGGi sce:YAL019W.

    Organism-specific databases

    CYGDi YAL019w.
    SGDi S000000017. FUN30.

    Phylogenomic databases

    eggNOGi COG0553.
    GeneTreei ENSGT00630000089890.
    HOGENOMi HOG000195581.
    KOi K14439.
    OMAi REFNKFC.
    OrthoDBi EOG7WDNB3.

    Enzyme and pathway databases

    BioCyci YEAST:G3O-28831-MONOMER.

    Miscellaneous databases

    NextBioi 968097.

    Gene expression databases

    Genevestigatori P31380.

    Family and domain databases

    Gene3Di 3.40.50.300. 2 hits.
    InterProi IPR014001. Helicase_ATP-bd.
    IPR001650. Helicase_C.
    IPR027417. P-loop_NTPase.
    IPR000330. SNF2_N.
    [Graphical view ]
    Pfami PF00271. Helicase_C. 1 hit.
    PF00176. SNF2_N. 1 hit.
    [Graphical view ]
    SMARTi SM00487. DEXDc. 1 hit.
    SM00490. HELICc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF52540. SSF52540. 3 hits.
    PROSITEi PS51192. HELICASE_ATP_BIND_1. 1 hit.
    PS51194. HELICASE_CTER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I."
      Clark M.W., Zhong W.-W., Keng T., Storms R.K., Barton A.B., Kaback D.B., Bussey H.
      Yeast 8:133-145(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    2. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
      Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
      Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    3. "Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region."
      Barton A.B., Kaback D.B.
      J. Bacteriol. 176:1872-1880(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    5. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    6. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    7. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
      Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
      J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Strain: ADR376.
    8. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-369, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    9. "The SNF2-family member Fun30 promotes gene silencing in heterochromatic loci."
      Neves-Costa A., Will W.R., Vetter A.T., Miller J.R., Varga-Weisz P.
      PLoS ONE 4:E8111-E8111(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "The Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme."
      Awad S., Ryan D., Prochasson P., Owen-Hughes T., Hassan A.H.
      J. Biol. Chem. 285:9477-9484(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBUNIT.
    12. "The Saccharomyces cerevisiae chromatin remodeler Fun30 regulates DNA end-resection and checkpoint deactivation."
      Eapen V.V., Sugawara N., Tsabar M., Wu W.H., Haber J.E.
      Mol. Cell. Biol. 32:4727-4740(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    13. "The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends."
      Chen X., Cui D., Papusha A., Zhang X., Chu C.D., Tang J., Chen K., Pan X., Ira G.
      Nature 489:576-580(2012) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
    14. Cited for: FUNCTION, MUTAGENESIS OF LYS-603.
    15. Cited for: FUNCTION.

    Entry informationi

    Entry nameiFUN30_YEAST
    AccessioniPrimary (citable) accession number: P31380
    Secondary accession number(s): D6VPJ9
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 127 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 6800 molecules/cell in log phase SD medium.1 Publication

    Caution

    Was initially reported to contain a CUE domain (PubMed:19956593). However, no CUE domain is predicted by prediction tools and the CUE-like region does not show no affinity for ubiquitinated histones (PubMed:20075079).2 Publications

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    3. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3