Uncharacterized ATP-dependent helicase FUN30 - Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

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P31380 (FUN30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 102. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Uncharacterized ATP-dependent helicase FUN30
EC=3.6.4.-

Gene names

Name:	FUN30
Ordered Locus Names:	YAL019W
ORF Names:	YAL001

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	1131 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	May function as a negative regulatory element in the process of UV damage repair.
Subcellular location	Nucleus Potential.
Miscellaneous	Present with 6800 molecules/cell in log phase SD medium. Ref.6
Sequence similarities	Belongs to the SNF2/RAD54 helicase family. Contains 1 helicase ATP-binding domain. Contains 1 helicase C-terminal domain.

Ontologies

Keywords
Cellular component	Nucleus
Ligand	ATP-binding DNA-binding Nucleotide-binding
Molecular function	Helicase Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	chromatin silencing at rDNA Inferred from mutant phenotype. Source: SGD chromatin silencing at silent mating-type cassette Inferred from physical interaction. Source: SGD chromatin silencing at telomere Inferred from mutant phenotype. Source: SGD heterochromatin assembly involved in chromatin silencing Inferred from mutant phenotype. Source: SGD heterochromatin maintenance involved in chromatin silencing Inferred from mutant phenotype. Source: SGD
Cellular component	mating-type region heterochromatin Inferred from physical interaction. Source: SGD mitochondrion Inferred from direct assay. Source: SGD nucleus Inferred from direct assay. Source: SGD
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA binding Inferred from direct assay. Source: SGD DNA-dependent ATPase activity Inferred from direct assay. Source: SGD chromatin binding Inferred from direct assay. Source: SGD helicase activity Inferred from electronic annotation. Source: UniProtKB-KW protein homodimerization activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1131

1131

Uncharacterized ATP-dependent helicase FUN30

PRO_0000074384

Regions

Domain

584 – 752

169

Helicase ATP-binding

Domain

953 – 1108

156

Helicase C-terminal

Nucleotide binding

597 – 604

ATP Potential

Motif

703 – 706

DEGH box

Amino acid modifications

Modified residue

Phosphoserine Ref.8 Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.7 Ref.8 Ref.9

Modified residue

Phosphothreonine Ref.9

Modified residue

Phosphoserine Ref.7 Ref.9

Modified residue

Phosphothreonine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

Phosphoserine Ref.9

Modified residue

131

Phosphothreonine Ref.9

Modified residue

226

Phosphoserine Ref.9

Modified residue

232

Phosphoserine Ref.9

Modified residue

369

Phosphoserine Ref.9

Modified residue

383

Phosphoserine Ref.9

Modified residue

451

Phosphoserine Ref.7 Ref.9

Sequences

Sequence

Length

Mass (Da)

Tools

P31380 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 7E61B9ABB3A42ED2
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FASTA

1,131

128,507

        10         20         30         40         50         60 
MSGSHSNDED DVVQVPETSS PTKVASSSPL KPTSPTVPDA SVASLRSRFT FKPSDPSEGA 

        70         80         90        100        110        120 
HTSKPLPSGS PEVALVNLAR EFPDFSQTLV QAVFKSNSFN LQSARERLTR LRQQRQNWTW 

       130        140        150        160        170        180 
NKNASPKKSE TPPPVKKSLP LANTGRLSSI HGNINNKSSK ITVAKQKTSI FDRYSNVINQ 

       190        200        210        220        230        240 
KQYTFELPTN LNIDSEALSK LPVNYNKKRR LVRADQHPIG KSYESSATQL GSAREKLLAN 

       250        260        270        280        290        300 
RKYGRHANDN DEEEEESMMT DDDDASGDDY TESTPQINLD EQVLQFINDS DIVDLSDLSD 

       310        320        330        340        350        360 
TTMHKAQLIA SHRPYSSLNA FVNTNFNDKD TEENASNKRK RRAAASANES ERLLDKITQS 

       370        380        390        400        410        420 
IRGYNAIESV IKKCSSYGDL VTSQMKKWGV QVEGDNSELD LMNLGEDDDD DNDDGNNDNN 

       430        440        450        460        470        480 
NSNNNNTAGA DATSKEKEDT KAVVEGFDET SAEPTPAPAP APVERETKRI RNTTKPKVVE 

       490        500        510        520        530        540 
DEDDDVDLEA IDDELPQSEH EDDDYEEEDE DYNDEEEDVE YDDGDDDDDD DDEFVATRKN 

       550        560        570        580        590        600 
THVISTTSRN GRKPIVKFFK GKPRLLSPEI SLKDYQQTGI NWLNLLYQNK MSCILADDMG 

       610        620        630        640        650        660 
LGKTCQVISF FAYLKQINEP GPHLVVVPSS TLENWLREFQ KFAPALKIEP YYGSLQEREE 

       670        680        690        700        710        720 
LRDILERNAG KYDVIVTTYN LAAGNKYDVS FLKNRNFNVV VYDEGHMLKN STSERFAKLM 

       730        740        750        760        770        780 
KIRANFRLLL TGTPLQNNLK ELMSLLEFIM PNLFISKKES FDAIFKQRAK TTDDNKNHNP 

       790        800        810        820        830        840 
LLAQEAITRA KTMMKPFILR RRKDQVLKHL PPKHTHIQYC ELNAIQKKIY DKEIQIVLEH 

       850        860        870        880        890        900 
KRMIKDGELP KDAKEKSKLQ SSSSKNLIMA LRKASLHPLL FRNIYNDKII TKMSDAILDE 

       910        920        930        940        950        960 
PAYAENGNKE YIKEDMSYMT DFELHKLCCN FPNTLSKYQL HNDEWMQSGK IDALKKLLKT 

       970        980        990       1000       1010       1020 
IIVDKQEKVL IFSLFTQVLD ILEMVLSTLD YKFLRLDGST QVNDRQLLID KFYEDKDIPI 

      1030       1040       1050       1060       1070       1080 
FILSTKAGGF GINLVCANNV IIFDQSFNPH DDRQAADRAH RVGQTKEVNI TTLITKDSIE 

      1090       1100       1110       1120       1130 
EKIHQLAKNK LALDSYISED KKSQDVLESK VSDMLEDIIY DENSKPKGTK E

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I." Clark M.W., Zhong W.-W., Keng T., Storms R.K., Barton A.B., Kaback D.B., Bussey H. Yeast 8:133-145(1992) [PubMed: 1561836] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	"Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes." Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H. Genome 36:32-42(1993) [PubMed: 8458570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	"Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region." Barton A.B., Kaback D.B. J. Bacteriol. 176:1872-1880(1994) [PubMed: 8144453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[6]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]	"Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway." Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N. Mol. Cell. Proteomics 4:310-327(2005) [PubMed: 15665377] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-28; SER-34 AND SER-451, MASS SPECTROMETRY. Strain: YAL6B.
[8]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed: 17563356] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26 AND SER-28, MASS SPECTROMETRY.
[9]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-26; SER-27; SER-28; THR-33; SER-34; THR-88; SER-96; SER-98; THR-131; SER-226; SER-232; SER-369; SER-383 AND SER-451, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L05146 Genomic DNA. Translation: AAC04938.1. BK006935 Genomic DNA. Translation: DAA06969.1.
PIR	S22266.
RefSeq	NP_009383.1. NM_001178164.1.
3D structure databases
ProteinModelPortal	P31380.
SMR	P31380. Positions 559-1106.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2541N.
IntAct	P31380. 12 interactions.
MINT	MINT-425278.
STRING	P31380.
Proteomic databases
PeptideAtlas	P31380.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL019W; YAL019W; YAL019W.
GeneID	851214.
KEGG	sce:YAL019W.
NMPDR	fig\|4932.3.peg.65.
Organism-specific databases
CYGD	YAL019w.
SGD	S000000017. FUN30.
Phylogenomic databases
eggNOG	fuNOG04339.
GeneTree	EFGT00050000000148.
HOGENOM	HBG398150.
OMA	KIEPYYG.
OrthoDB	EOG4BCHW4.
Gene expression databases
ArrayExpress	P31380.
Genevestigator	P31380.
GermOnline	YAL019W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR014001. DEAD-like_helicase. IPR001650. Helicase_C. IPR000330. SNF2_N. [Graphical view]
KO	K14439.
Pfam	PF00271. Helicase_C. 1 hit. PF00176. SNF2_N. 1 hit. [Graphical view]
SMART	SM00487. DEXDc. 1 hit. SM00490. HELICc. 1 hit. [Graphical view]
PROSITE	PS51192. HELICASE_ATP_BIND_1. 1 hit. PS51194. HELICASE_CTER. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968097.

Entry information

Entry name

FUN30_YEAST

Accession

Primary (citable) accession number: P31380
Secondary accession number(s): D6VPJ9

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	January 25, 2012
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program

Relevant documents

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Amino acid modifications

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents