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Protein

ATP-dependent helicase FUN30

Gene

FUN30

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs, facilitating single-stranded DNA (ssDNA) production by the EXO1 and SGS1 machinery. Promotes gene silencing at heterochromatin by regulating the chromatin structure within or around silent loci. Also required for heterochromatin organization at centromeres.6 Publications

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

ATP + H2O = ADP + phosphate.

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi597 – 604ATPCurated8

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • chromatin binding Source: SGD
  • DNA binding Source: SGD
  • DNA-dependent ATPase activity Source: SGD
  • helicase activity Source: UniProtKB-KW
  • identical protein binding Source: IntAct

GO - Biological processi

  • ATP-dependent chromatin remodeling Source: SGD
  • chromatin remodeling Source: SGD
  • chromatin silencing at rDNA Source: SGD
  • chromatin silencing at silent mating-type cassette Source: SGD
  • chromatin silencing at telomere Source: SGD
  • covalent chromatin modification Source: UniProtKB-KW
  • DNA double-strand break processing Source: SGD
  • heterochromatin assembly involved in chromatin silencing Source: SGD
  • heterochromatin maintenance involved in chromatin silencing Source: SGD
  • negative regulation of transcription from RNA polymerase II promoter Source: SGD
  • nucleosome mobilization Source: SGD
  • nucleosome positioning Source: SGD

Keywordsi

Molecular functionChromatin regulator, DNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28831-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
ATP-dependent helicase FUN30 (EC:3.6.4.12)
Gene namesi
Name:FUN30
Ordered Locus Names:YAL019W
ORF Names:YAL001
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL019W.
SGDiS000000017. FUN30.

Subcellular locationi

GO - Cellular componenti

  • chromosome, centromeric region Source: SGD
  • chromosome, telomeric region Source: GOC
  • mating-type region heterochromatin Source: SGD
  • nucleus Source: UniProtKB-SubCell

Keywords - Cellular componenti

Chromosome, Nucleus

Pathology & Biotechi

Disruption phenotypei

Impaired ability to repair double-strand breaks (DSBs).1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi603K → R: Abolishes both the silencing function and the ability to promote DNA end resection of double-strand breaks (DSBs). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000743841 – 1131ATP-dependent helicase FUN30Add BLAST1131

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei232PhosphoserineCombined sources1
Modified residuei369PhosphoserineCombined sources1
Modified residuei451PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31380.
PRIDEiP31380.

PTM databases

iPTMnetiP31380.

Interactioni

Subunit structurei

Homodimer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself6EBI-20621,EBI-20621

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi31747. 190 interactors.
DIPiDIP-2541N.
IntActiP31380. 10 interactors.
MINTiMINT-425278.
STRINGi4932.YAL019W.

Structurei

Secondary structure

11131
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi813 – 820Combined sources8
Helixi824 – 846Combined sources23
Helixi853 – 858Combined sources6
Helixi859 – 861Combined sources3
Helixi864 – 874Combined sources11
Helixi878 – 880Combined sources3
Beta strandi883 – 885Combined sources3
Helixi887 – 897Combined sources11
Helixi901 – 904Combined sources4
Helixi909 – 917Combined sources9
Helixi921 – 930Combined sources10
Helixi932 – 935Combined sources4
Helixi936 – 938Combined sources3
Helixi944 – 946Combined sources3
Helixi949 – 962Combined sources14
Beta strandi969 – 974Combined sources6
Helixi976 – 988Combined sources13
Beta strandi993 – 996Combined sources4
Turni1002 – 1004Combined sources3
Helixi1005 – 1014Combined sources10
Beta strandi1020 – 1024Combined sources5
Helixi1025 – 1029Combined sources5
Beta strandi1039 – 1044Combined sources6
Helixi1049 – 1056Combined sources8
Turni1057 – 1059Combined sources3
Beta strandi1068 – 1075Combined sources8
Helixi1079 – 1086Combined sources8
Helixi1094 – 1096Combined sources3
Helixi1109 – 1122Combined sources14

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
5GN1X-ray1.95A/B/C/D780-1122[»]
ProteinModelPortaliP31380.
SMRiP31380.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini584 – 752Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST169
Domaini953 – 1108Helicase C-terminalPROSITE-ProRule annotationAdd BLAST156

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni76 – 111CUE-like regionAdd BLAST36

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi703 – 706DEGH box4

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Phylogenomic databases

GeneTreeiENSGT00890000139433.
HOGENOMiHOG000195581.
InParanoidiP31380.
KOiK14439.
OMAiIVYDEGH.
OrthoDBiEOG092C0RAA.

Family and domain databases

InterProiView protein in InterPro
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SUPFAMiSSF52540. SSF52540. 3 hits.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.

Sequencei

Sequence statusi: Complete.

P31380-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSGSHSNDED DVVQVPETSS PTKVASSSPL KPTSPTVPDA SVASLRSRFT
60 70 80 90 100
FKPSDPSEGA HTSKPLPSGS PEVALVNLAR EFPDFSQTLV QAVFKSNSFN
110 120 130 140 150
LQSARERLTR LRQQRQNWTW NKNASPKKSE TPPPVKKSLP LANTGRLSSI
160 170 180 190 200
HGNINNKSSK ITVAKQKTSI FDRYSNVINQ KQYTFELPTN LNIDSEALSK
210 220 230 240 250
LPVNYNKKRR LVRADQHPIG KSYESSATQL GSAREKLLAN RKYGRHANDN
260 270 280 290 300
DEEEEESMMT DDDDASGDDY TESTPQINLD EQVLQFINDS DIVDLSDLSD
310 320 330 340 350
TTMHKAQLIA SHRPYSSLNA FVNTNFNDKD TEENASNKRK RRAAASANES
360 370 380 390 400
ERLLDKITQS IRGYNAIESV IKKCSSYGDL VTSQMKKWGV QVEGDNSELD
410 420 430 440 450
LMNLGEDDDD DNDDGNNDNN NSNNNNTAGA DATSKEKEDT KAVVEGFDET
460 470 480 490 500
SAEPTPAPAP APVERETKRI RNTTKPKVVE DEDDDVDLEA IDDELPQSEH
510 520 530 540 550
EDDDYEEEDE DYNDEEEDVE YDDGDDDDDD DDEFVATRKN THVISTTSRN
560 570 580 590 600
GRKPIVKFFK GKPRLLSPEI SLKDYQQTGI NWLNLLYQNK MSCILADDMG
610 620 630 640 650
LGKTCQVISF FAYLKQINEP GPHLVVVPSS TLENWLREFQ KFAPALKIEP
660 670 680 690 700
YYGSLQEREE LRDILERNAG KYDVIVTTYN LAAGNKYDVS FLKNRNFNVV
710 720 730 740 750
VYDEGHMLKN STSERFAKLM KIRANFRLLL TGTPLQNNLK ELMSLLEFIM
760 770 780 790 800
PNLFISKKES FDAIFKQRAK TTDDNKNHNP LLAQEAITRA KTMMKPFILR
810 820 830 840 850
RRKDQVLKHL PPKHTHIQYC ELNAIQKKIY DKEIQIVLEH KRMIKDGELP
860 870 880 890 900
KDAKEKSKLQ SSSSKNLIMA LRKASLHPLL FRNIYNDKII TKMSDAILDE
910 920 930 940 950
PAYAENGNKE YIKEDMSYMT DFELHKLCCN FPNTLSKYQL HNDEWMQSGK
960 970 980 990 1000
IDALKKLLKT IIVDKQEKVL IFSLFTQVLD ILEMVLSTLD YKFLRLDGST
1010 1020 1030 1040 1050
QVNDRQLLID KFYEDKDIPI FILSTKAGGF GINLVCANNV IIFDQSFNPH
1060 1070 1080 1090 1100
DDRQAADRAH RVGQTKEVNI TTLITKDSIE EKIHQLAKNK LALDSYISED
1110 1120 1130
KKSQDVLESK VSDMLEDIIY DENSKPKGTK E
Length:1,131
Mass (Da):128,507
Last modified:July 1, 1993 - v1
Checksum:i7E61B9ABB3A42ED2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04938.1.
BK006935 Genomic DNA. Translation: DAA06969.1.
PIRiS22266.
RefSeqiNP_009383.1. NM_001178164.1.

Genome annotation databases

EnsemblFungiiYAL019W; YAL019W; YAL019W.
GeneIDi851214.
KEGGisce:YAL019W.

Similar proteinsi

Entry informationi

Entry nameiFUN30_YEAST
AccessioniPrimary (citable) accession number: P31380
Secondary accession number(s): D6VPJ9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: August 30, 2017
This is version 154 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Caution

Was initially reported to contain a CUE domain (PubMed:19956593). However, no CUE domain is predicted by prediction tools and the CUE-like region does not show no affinity for ubiquitinated histones (PubMed:20075079).2 Publications

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families
  3. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  4. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names