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P31380 (FUN30_YEAST) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 125. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
ATP-dependent helicase FUN30

EC=3.6.4.12
Gene names
Name:FUN30
Ordered Locus Names:YAL019W
ORF Names:YAL001
OrganismSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]
Taxonomic identifier559292 [NCBI]
Taxonomic lineageEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces

Protein attributes

Sequence length1131 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

DNA helicase that possesses intrinsic ATP-dependent nucleosome-remodeling activity and is both required for DNA repair and heterochromatin organization. Promotes DNA end resection of double-strand breaks (DSBs) following DNA damage: probably acts by weakening histone DNA interactions in nucleosomes flanking DSBs, facilitating single-stranded DNA (ssDNA) production by the EXO1 and SGS1 machinery. Promotes gene silencing at heterochromatin by regulating the chromatin structure within or around silent loci. Also required for heterochromatin organization at centromeres. Ref.9 Ref.11 Ref.12 Ref.13 Ref.14 Ref.15

Catalytic activity

ATP + H2O = ADP + phosphate.

Subunit structure

Homodimer. Ref.11

Subcellular location

Nucleus. Chromosome. Note: Recruited to double-strand breaks (DSBs) sites of DNA damage. Ref.12 Ref.13

Disruption phenotype

Impaired ability to repair double-strand breaks (DSBs). Ref.13

Miscellaneous

Present with 6800 molecules/cell in log phase SD medium.

Sequence similarities

Belongs to the SNF2/RAD54 helicase family.

Contains 1 helicase ATP-binding domain.

Contains 1 helicase C-terminal domain.

Caution

Was initially reported to contain a CUE domain (Ref.9). However, no CUE domain is predicted by prediction tools and the CUE-like region does not show no affinity for ubiquitinated histones (Ref.11).

Ontologies

Keywords
   Biological processDNA damage
DNA repair
   Cellular componentChromosome
Nucleus
   LigandATP-binding
DNA-binding
Nucleotide-binding
   Molecular functionChromatin regulator
Helicase
Hydrolase
   PTMPhosphoprotein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processATP catabolic process

Inferred from direct assay Ref.11. Source: GOC

ATP-dependent chromatin remodeling

Inferred from mutant phenotype Ref.11. Source: SGD

DNA double-strand break processing

Inferred from mutant phenotype Ref.13Ref.14. Source: SGD

chromatin silencing at rDNA

Inferred from mutant phenotype Ref.9. Source: SGD

chromatin silencing at silent mating-type cassette

Inferred from mutant phenotype Ref.9. Source: SGD

chromatin silencing at telomere

Inferred from mutant phenotype Ref.9. Source: SGD

heterochromatin assembly involved in chromatin silencing

Inferred from mutant phenotype PubMed 21388962. Source: SGD

heterochromatin maintenance involved in chromatin silencing

Inferred from mutant phenotype PubMed 21388962. Source: SGD

   Cellular_componentchromosome, centromeric region

Inferred from direct assay Ref.15. Source: SGD

mating-type region heterochromatin

Inferred from physical interaction Ref.9. Source: SGD

nucleus

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

DNA binding

Inferred from direct assay Ref.11. Source: SGD

DNA-dependent ATPase activity

Inferred from direct assay Ref.11. Source: SGD

chromatin binding

Inferred from direct assay Ref.11. Source: SGD

helicase activity

Inferred from electronic annotation. Source: UniProtKB-KW

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11311131ATP-dependent helicase FUN30
PRO_0000074384

Regions

Domain584 – 752169Helicase ATP-binding
Domain953 – 1108156Helicase C-terminal
Nucleotide binding597 – 6048ATP Probable
Region76 – 11136CUE-like region
Motif703 – 7064DEGH box

Amino acid modifications

Modified residue2321Phosphoserine Ref.8
Modified residue3691Phosphoserine Ref.8
Modified residue4511Phosphoserine Ref.10

Experimental info

Mutagenesis6031K → R: Abolishes both the silencing function and the ability to promote DNA end resection of double-strand breaks (DSBs). Ref.14

Sequences

Sequence LengthMass (Da)Tools
P31380 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 7E61B9ABB3A42ED2

FASTA1,131128,507
        10         20         30         40         50         60 
MSGSHSNDED DVVQVPETSS PTKVASSSPL KPTSPTVPDA SVASLRSRFT FKPSDPSEGA 

        70         80         90        100        110        120 
HTSKPLPSGS PEVALVNLAR EFPDFSQTLV QAVFKSNSFN LQSARERLTR LRQQRQNWTW 

       130        140        150        160        170        180 
NKNASPKKSE TPPPVKKSLP LANTGRLSSI HGNINNKSSK ITVAKQKTSI FDRYSNVINQ 

       190        200        210        220        230        240 
KQYTFELPTN LNIDSEALSK LPVNYNKKRR LVRADQHPIG KSYESSATQL GSAREKLLAN 

       250        260        270        280        290        300 
RKYGRHANDN DEEEEESMMT DDDDASGDDY TESTPQINLD EQVLQFINDS DIVDLSDLSD 

       310        320        330        340        350        360 
TTMHKAQLIA SHRPYSSLNA FVNTNFNDKD TEENASNKRK RRAAASANES ERLLDKITQS 

       370        380        390        400        410        420 
IRGYNAIESV IKKCSSYGDL VTSQMKKWGV QVEGDNSELD LMNLGEDDDD DNDDGNNDNN 

       430        440        450        460        470        480 
NSNNNNTAGA DATSKEKEDT KAVVEGFDET SAEPTPAPAP APVERETKRI RNTTKPKVVE 

       490        500        510        520        530        540 
DEDDDVDLEA IDDELPQSEH EDDDYEEEDE DYNDEEEDVE YDDGDDDDDD DDEFVATRKN 

       550        560        570        580        590        600 
THVISTTSRN GRKPIVKFFK GKPRLLSPEI SLKDYQQTGI NWLNLLYQNK MSCILADDMG 

       610        620        630        640        650        660 
LGKTCQVISF FAYLKQINEP GPHLVVVPSS TLENWLREFQ KFAPALKIEP YYGSLQEREE 

       670        680        690        700        710        720 
LRDILERNAG KYDVIVTTYN LAAGNKYDVS FLKNRNFNVV VYDEGHMLKN STSERFAKLM 

       730        740        750        760        770        780 
KIRANFRLLL TGTPLQNNLK ELMSLLEFIM PNLFISKKES FDAIFKQRAK TTDDNKNHNP 

       790        800        810        820        830        840 
LLAQEAITRA KTMMKPFILR RRKDQVLKHL PPKHTHIQYC ELNAIQKKIY DKEIQIVLEH 

       850        860        870        880        890        900 
KRMIKDGELP KDAKEKSKLQ SSSSKNLIMA LRKASLHPLL FRNIYNDKII TKMSDAILDE 

       910        920        930        940        950        960 
PAYAENGNKE YIKEDMSYMT DFELHKLCCN FPNTLSKYQL HNDEWMQSGK IDALKKLLKT 

       970        980        990       1000       1010       1020 
IIVDKQEKVL IFSLFTQVLD ILEMVLSTLD YKFLRLDGST QVNDRQLLID KFYEDKDIPI 

      1030       1040       1050       1060       1070       1080 
FILSTKAGGF GINLVCANNV IIFDQSFNPH DDRQAADRAH RVGQTKEVNI TTLITKDSIE 

      1090       1100       1110       1120       1130 
EKIHQLAKNK LALDSYISED KKSQDVLESK VSDMLEDIIY DENSKPKGTK E 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I."
Clark M.W., Zhong W.-W., Keng T., Storms R.K., Barton A.B., Kaback D.B., Bussey H.
Yeast 8:133-145(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[2]"Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[3]"Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region."
Barton A.B., Kaback D.B.
J. Bacteriol. 176:1872-1880(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: ATCC 204511 / S288c / AB972.
[4]"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae."
Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K.
Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 204508 / S288c.
[5]"The reference genome sequence of Saccharomyces cerevisiae: Then and now."
Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R., Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S., Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.
G3 (Bethesda) 4:389-398(2014) [PubMed] [Europe PMC] [Abstract]
Cited for: GENOME REANNOTATION.
Strain: ATCC 204508 / S288c.
[6]"Global analysis of protein expression in yeast."
Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S.
Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[7]"Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
Strain: ADR376.
[8]"A multidimensional chromatography technology for in-depth phosphoproteome analysis."
Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-232 AND SER-369, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[9]"The SNF2-family member Fun30 promotes gene silencing in heterochromatic loci."
Neves-Costa A., Will W.R., Vetter A.T., Miller J.R., Varga-Weisz P.
PLoS ONE 4:E8111-E8111(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[10]"Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-451, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
[11]"The Snf2 homolog Fun30 acts as a homodimeric ATP-dependent chromatin-remodeling enzyme."
Awad S., Ryan D., Prochasson P., Owen-Hughes T., Hassan A.H.
J. Biol. Chem. 285:9477-9484(2010) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBUNIT.
[12]"The Saccharomyces cerevisiae chromatin remodeler Fun30 regulates DNA end-resection and checkpoint deactivation."
Eapen V.V., Sugawara N., Tsabar M., Wu W.H., Haber J.E.
Mol. Cell. Biol. 32:4727-4740(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[13]"The Fun30 nucleosome remodeller promotes resection of DNA double-strand break ends."
Chen X., Cui D., Papusha A., Zhang X., Chu C.D., Tang J., Chen K., Pan X., Ira G.
Nature 489:576-580(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION, DISRUPTION PHENOTYPE.
[14]"The yeast Fun30 and human SMARCAD1 chromatin remodellers promote DNA end resection."
Costelloe T., Louge R., Tomimatsu N., Mukherjee B., Martini E., Khadaroo B., Dubois K., Wiegant W.W., Thierry A., Burma S., van Attikum H., Llorente B.
Nature 489:581-584(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, MUTAGENESIS OF LYS-603.
[15]"SWI/SNF-Like chromatin remodeling factor Fun30 supports point centromere function in S. cerevisiae."
Durand-Dubief M., Will W.R., Petrini E., Theodorou D., Harris R.R., Crawford M.R., Paszkiewicz K., Krueger F., Correra R.M., Vetter A.T., Miller J.R., Kent N.A., Varga-Weisz P.
PLoS Genet. 8:E1002974-E1002974(2012) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L05146 Genomic DNA. Translation: AAC04938.1.
BK006935 Genomic DNA. Translation: DAA06969.1.
PIRS22266.
RefSeqNP_009383.1. NM_001178164.1.

3D structure databases

ProteinModelPortalP31380.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid31747. 88 interactions.
DIPDIP-2541N.
IntActP31380. 10 interactions.
MINTMINT-425278.
STRING4932.YAL019W.

Proteomic databases

MaxQBP31380.
PaxDbP31380.
PeptideAtlasP31380.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblFungiYAL019W; YAL019W; YAL019W.
GeneID851214.
KEGGsce:YAL019W.

Organism-specific databases

CYGDYAL019w.
SGDS000000017. FUN30.

Phylogenomic databases

eggNOGCOG0553.
GeneTreeENSGT00630000089890.
HOGENOMHOG000195581.
KOK14439.
OMAREFNKFC.
OrthoDBEOG7WDNB3.

Enzyme and pathway databases

BioCycYEAST:G3O-28831-MONOMER.

Gene expression databases

GenevestigatorP31380.

Family and domain databases

Gene3D3.40.50.300. 2 hits.
InterProIPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
[Graphical view]
PfamPF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
[Graphical view]
SMARTSM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
[Graphical view]
SUPFAMSSF52540. SSF52540. 3 hits.
PROSITEPS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio968097.

Entry information

Entry nameFUN30_YEAST
AccessionPrimary (citable) accession number: P31380
Secondary accession number(s): D6VPJ9
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 14, 2014
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Relevant documents

Yeast chromosome I

Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Yeast

Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD

SIMILARITY comments

Index of protein domains and families