P31377 (STX8_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Syntaxin-8

Alternative name(s):
SNARE protein related to mammalian syntaxin 8
ULP1-interacting protein 2

Gene names

Name:	SYN8
Synonyms:	UIP2
Ordered Locus Names:	YAL014C
ORF Names:	FUN34

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	255 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	t-SNARE which may play a role in determining the specificity of membrane fusion, protein transport and vesicle trafficking within the Golgi/endosomal and plasma membrane/endosomal systems. Ref.1
Subunit structure	Interacts with PEP12, VTI1 and the SNC SNARE complex proteins. Ref.1
Subcellular location	Endosome membrane; Single-pass type IV membrane protein Ref.1.
Post-translational modification	Palmitoylated by SWF1. Ref.8 Ref.9
Sequence similarities	Belongs to the syntaxin family. Contains 1 t-SNARE coiled-coil homology domain.

Ontologies

Keywords
Biological process	Protein transport Transport
Cellular component	Endosome Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Lipoprotein Palmitate
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	endosome membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	SNAP receptor activity Inferred from direct assay Ref.1. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 255	255	Syntaxin-8		PRO_0000210281
Regions
	Topological domain	1 – 234	234	Cytoplasmic Potential
	Transmembrane	235 – 255	21	Helical; Anchor for type IV membrane protein; Potential
	Domain	164 – 228	65	t-SNARE coiled-coil homology
Amino acid modifications
	Lipidation	238	1	S-palmitoyl cysteine Probable

Sequences

	Sequence		Length	Mass (Da)
	P31377 [UniParc]. Last modified May 16, 2003. Version 2. Checksum: 7631FB672F8E55D6	FASTA	255	29,049
	10 20 30 40 50 60 MDVLKLGYEL DQLSDLVEER TRLVSVLKLA PTSNDNVTLK RQLGSILELL QKCAPNDELI 70 80 90 100 110 120 SRYNTILDKI PDTAVDKELY RFQQQVARNT DEVSKESLKK VRFKNDDELT VMYKDDDEQD 130 140 150 160 170 180 EESPLPSTHT PYKDEPLQSQ LQSQSQPQPP QPMVSNQELF INQQQQLLEQ DSHLGALSQS 190 200 210 220 230 240 IGRTHDISLD LNNEIVSQND SLLVDLENLI DNNGRNLNRA SRSMHGFNNS RFKDNGNCVI 250 ILVLIVVLLL LLLVL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A new yeast endosomal SNARE related to mammalian syntaxin 8." Lewis M.J., Pelham H.R.B. Traffic 3:922-929(2002) [PubMed: 12453154] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION, SUBCELLULAR LOCATION, INTERACTION WITH PEP12; VTI1 AND SNC. Strain: ATCC 201389 / BY4742.
[2]	"Re-sequencing of the UIP2 gene reveals a frame-correction and a new Qc-SNARE in the endosome of Saccharomyces cerevisiae." Burri L., Hofmann K., Lithgow T. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes." Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H. Genome 36:32-42(1993) [PubMed: 8458570] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"Molecular cloning of chromosome I DNA from Saccharomyces cerevisiae: analysis of the genes in the FUN38-MAK16-SPO7 region." Barton A.B., Kaback D.B. J. Bacteriol. 176:1872-1880(1994) [PubMed: 8144453] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[6]	Dolinski K.J., Cherry J.M. Submitted (DEC-2002) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION TO C-TERMINUS.
[7]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[8]	"Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation." Valdez-Taubas J., Pelham H.R.B. EMBO J. 24:2524-2532(2005) [PubMed: 15973437] [Abstract] Cited for: PALMITOYLATION.
[9]	"Global analysis of protein palmitoylation in yeast." Roth A.F., Wan J., Bailey A.O., Sun B., Kuchar J.A., Green W.N., Phinney B.S., Yates J.R. III, Davis N.G. Cell 125:1003-1013(2006) [PubMed: 16751107] [Abstract] Cited for: PALMITOYLATION.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY205237 Genomic DNA. Translation: AAO46883.1. AY205236 Genomic DNA. Translation: AAO22142.1. L05146 Genomic DNA. Translation: AAC04943.2. BK006935 Genomic DNA. Translation: DAA06974.1.
PIR	S36720.
RefSeq	NP_009388.2. NM_001178159.1.
3D structure databases
ProteinModelPortal	P31377.
SMR	P31377. Positions 171-224.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5642N.
IntAct	P31377. 8 interactions.
MINT	MINT-485478.
STRING	P31377.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL014C; YAL014C; YAL014C.
GeneID	851219.
KEGG	sce:YAL014C.
NMPDR	fig|4932.3.peg.72.
Organism-specific databases
CYGD	YAL014c.
SGD	S000000012. SYN8.
Phylogenomic databases
eggNOG	fuNOG10877.
GeneTree	EFGT00050000003320.
HOGENOM	HBG397920.
OMA	DKELYRF.
OrthoDB	EOG4HTD29.
Gene expression databases
ArrayExpress	P31377.
Genevestigator	P31377.
GermOnline	YAL014C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000727. T_SNARE_dom. [Graphical view]
KO	K08501.
Pfam	PF05739. SNARE. 1 hit. [Graphical view]
PROSITE	PS50192. T_SNARE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	968112.

Entry information

Entry name

STX8_YEAST

Accession

Primary (citable) accession number: P31377
Secondary accession number(s): D6VPK4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	May 16, 2003
Last modified:	December 14, 2011
This is version 92 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program