P31376 (SWC3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
SWR1-complex protein 3

Gene names

Name:	SWC3
Synonyms:	FUN36, SWC1
Ordered Locus Names:	YAL011W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) [Reference proteome]

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Fungi › Dikarya › Ascomycota › saccharomyceta › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces › Saccharomyces cerevisiae

Protein attributes

Sequence length	625 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Component of the SWR1 complex which mediates the ATP-dependent exchange of histone H2A for the H2A variant HZT1 leading to transcriptional regulation of selected genes by chromatin remodeling. Involved in chromosome stability. Ref.4 Ref.8 Ref.9
Subunit structure	Component of the SWR1 chromatin remodeling complex composed of at least ACT1, ARP4, RVB1, RVB2, ARP6, YAF9, VPS71, VPS72, SWC3, SWC4, SWC5, SWC7 and SWR1, and perhaps BDF1. Ref.4 Ref.8 Ref.9
Subcellular location	Nucleus Ref.6.
Miscellaneous	Present with 1380 molecules/cell in log phase SD medium.
Sequence similarities	Belongs to the SWC3 family.
Sequence caution	The sequence AAC04946.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Transcription Transcription regulation
Cellular component	Nucleus
Molecular function	Activator Chromatin regulator
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	endoplasmic reticulum organization Inferred from mutant phenotype PubMed 12868057. Source: SGD histone exchange Inferred from physical interaction PubMed 16299513. Source: SGD regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW transcription, DNA-dependent Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	Swr1 complex Inferred from direct assay Ref.9Ref.4. Source: SGD mitochondrion Inferred from direct assay PubMed 14576278PubMed 16823961. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 625	625	SWR1-complex protein 3		PRO_0000072344
Regions
	Compositional bias	34 – 56	23	Asp-rich
	Compositional bias	175 – 262	88	Lys-rich
Amino acid modifications
	Modified residue	139	1	Phosphoserine Ref.11
	Modified residue	142	1	Phosphoserine Ref.11
	Modified residue	268	1	Phosphoserine Ref.10
	Modified residue	523	1	Phosphoserine Ref.11

Sequences

	Sequence		Length	Mass (Da)
	P31376 [UniParc]. Last modified January 16, 2004. Version 2. Checksum: A4F13DB85B657D59	FASTA	625	72,514
	10 20 30 40 50 60 MPAVLRTRSK ESSIEQKPAS RTRTRSRRGK RGRDDDDDDD DEESDDAYDE VGNDYDEYAS 70 80 90 100 110 120 RAKLATNRPF EIVAGLPASV ELPNYNSSLT HPQSIKNSGV LYDSLVSSRR TWVQGEMFEL 130 140 150 160 170 180 YWRRPKKIVS ESTPAATESP TSGTIPLIRD KMQKMCDCVM SGGPHTFKVR LFILKNDKIE 190 200 210 220 230 240 QKWQDEQELK KKEKELKRKN DAEAKRLRME ERKRQQMQKK IAKEQKLQLQ KENKAKQKLE 250 260 270 280 290 300 QEALKLKRKE EMKKLKEQNK NKQGSPSSSM HDPRMIMNLN LMAQEDPKLN TLMETVAKGL 310 320 330 340 350 360 ANNSQLEEFK KFIEIAKKRS LEENPVNKRP SVTTTRPAPP SKAKDVAEDH RLNSITLVKS 370 380 390 400 410 420 SKTAATEPEP KKADDENAEK QQSKEAKTTA ESTQVDVKKE EEDVKEKGVK SEDTQKKEDN 430 440 450 460 470 480 QVVPKRKRRK NAIKEDKDMQ LTAFQQKYVQ GAEIILEYLE FTHSRYYLPK KSVVEFLEDT 490 500 510 520 530 540 DEIIISWIVI HNSKEIEKFK TKKIKAKLKA DQKLNKEDAK PGSDVEKEVS FNPLFEADCP 550 560 570 580 590 600 TPLYTPMTMK LSGIHKRFNQ IIRNSVSPME EVVKEMEKIL QIGTRLSGYN LWYQLDGYDD 610 620 EALSESLRFE LNEWEHAMRS RRHKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes." Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H. Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[2]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	"A Snf2 family ATPase complex required for recruitment of the histone H2A variant Htz1." Krogan N.J., Keogh M.-C., Datta N., Sawa C., Ryan O.W., Ding H., Haw R.A., Pootoolal J., Tong A., Canadien V., Richards D.P., Wu X., Emili A., Hughes T.R., Buratowski S., Greenblatt J.F. Mol. Cell 12:1565-1576(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[5]	"Sequencing and comparison of yeast species to identify genes and regulatory elements." Kellis M., Patterson N., Endrizzi M., Birren B.W., Lander E.S. Nature 423:241-254(2003) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION OF PROBABLE INITIATION SITE.
[6]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed] [Europe PMC] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed] [Europe PMC] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A protein complex containing the conserved Swi2/Snf2-related ATPase Swr1p deposits histone variant H2A.Z into euchromatin." Kobor M.S., Venkatasubrahmanyam S., Meneghini M.D., Gin J.W., Jennings J.L., Link A.J., Madhani H.D., Rine J. PLoS Biol. 2:587-599(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[9]	"ATP-driven exchange of histone H2AZ variant catalyzed by SWR1 chromatin remodeling complex." Mizuguchi G., Shen X., Landry J., Wu W.-H., Sen S., Wu C. Science 303:343-348(2004) [PubMed] [Europe PMC] [Abstract] Cited for: IDENTIFICATION IN THE SWR1 COMPLEX, FUNCTION OF THE SWR1 COMPLEX, MASS SPECTROMETRY.
[10]	"Proteome-wide identification of in vivo targets of DNA damage checkpoint kinases." Smolka M.B., Albuquerque C.P., Chen S.H., Zhou H. Proc. Natl. Acad. Sci. U.S.A. 104:10364-10369(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-268, MASS SPECTROMETRY.
[11]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-139; SER-142 AND SER-523, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L05146 Genomic DNA. Translation: AAC04946.1. Different initiation. BK006935 Genomic DNA. Translation: DAA06977.1.
PIR	S36723.
RefSeq	NP_009391.2. NM_001178156.1.
3D structure databases
ProteinModelPortal	P31376.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-2986N.
IntAct	P31376. 20 interactions.
MINT	MINT-437491.
STRING	4932.YAL011W.
Proteomic databases
PaxDb	P31376.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YAL011W; YAL011W; YAL011W.
GeneID	851222.
KEGG	sce:YAL011W.
Organism-specific databases
CYGD	YAL011w.
SGD	S000000009. SWC3.
Phylogenomic databases
eggNOG	NOG47360.
HOGENOM	HOG000001103.
KO	K11682.
OMA	NLWYQLD.
OrthoDB	EOG444PVB.
Enzyme and pathway databases
BioCyc	YEAST:G3O-28824-MONOMER.
Gene expression databases
Genevestigator	P31376.
GermOnline	YAL011W. Saccharomyces cerevisiae.
Family and domain databases
ProtoNet	Search...
Other
NextBio	968121.

Entry information

Entry name

SWC3_YEAST

Accession

Primary (citable) accession number: P31376
Secondary accession number(s): D6VPK7

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 16, 2004
Last modified:	May 29, 2013
This is version 95 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program