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P31374

- PSK1_YEAST

UniProt

P31374 - PSK1_YEAST

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Protein

Serine/threonine-protein kinase PSK1

Gene
PSK1, YAL017W, FUN31, YAL002
Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the control of sugar metabolism and translation. Phosphorylates UGP1, which is required for normal glycogen and beta-(1,6)-glucan synthesis. This phosphorylation shifts glucose partitioning toward cell wall glucan synthesis at the expense of glycogen synthesis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1125 – 11251ATP By similarity
Active sitei1230 – 12301Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1102 – 11109ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: SGD
  3. signal transducer activity Source: InterPro

GO - Biological processi

  1. negative regulation of glycogen biosynthetic process Source: SGD
  2. protein phosphorylation Source: SGD
  3. regulation of (1->6)-beta-D-glucan biosynthetic process Source: SGD
  4. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28829-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PSK1 (EC:2.7.11.1)
Alternative name(s):
PAS kinase 1
Gene namesi
Name:PSK1
Ordered Locus Names:YAL017W
ORF Names:FUN31, YAL002
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL017w.
SGDiS000000015. PSK1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13561356Serine/threonine-protein kinase PSK1PRO_0000086042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Phosphoserine1 Publication
Modified residuei192 – 1921Phosphoserine1 Publication
Modified residuei202 – 2021Phosphoserine1 Publication
Modified residuei255 – 2551Phosphoserine1 Publication
Modified residuei286 – 2861Phosphoserine1 Publication
Modified residuei327 – 3271Phosphoserine1 Publication
Modified residuei926 – 9261Phosphoserine1 Publication
Modified residuei1018 – 10181Phosphoserine1 Publication
Modified residuei1023 – 10231Phosphoserine1 Publication
Modified residuei1035 – 10351Phosphoserine2 Publications
Modified residuei1055 – 10551Phosphoserine2 Publications
Modified residuei1079 – 10791Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31374.
PaxDbiP31374.
PeptideAtlasiP31374.

Expressioni

Gene expression databases

GenevestigatoriP31374.

Interactioni

Protein-protein interaction databases

BioGridi31749. 77 interactions.
DIPiDIP-6267N.
IntActiP31374. 16 interactions.
MINTiMINT-604653.
STRINGi4932.YAL017W.

Structurei

3D structure databases

ProteinModelPortaliP31374.
SMRiP31374. Positions 1055-1354.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini450 – 51869PAS 1Add
BLAST
Domaini738 – 80770PAS 2Add
BLAST
Domaini1096 – 1354259Protein kinaseAdd
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117599.
KOiK08286.
OMAiSTHAYSY.
OrthoDBiEOG7G4QPK.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31374-1 [UniParc]FASTAAdd to Basket

« Hide

MPYIGASNLS EHSFVNLKEK HAITHKGTSS SVASLQTPPS PDQENHIDNE     50
LENYDTSLSD VSTPNKKEGD EFEQSLRDTF ASFRKTKPPP PLDFEQPRLP 100
STASSSVDST VSSPLTDEDI KELEFLPNES THSYSYNPLS PNSLAVRLRI 150
LKRSLEIIIQ NPSMLLEPTP DDLPPLKEFA GRRSSLPRTS ASANHLMNRN 200
KSQIWNTTSA TLNAFVNNTS SSSAASSALS NKKPGTPVFP NLDPTHSQTF 250
HRANSLAYLP SILPEQDPLL KHNNSLFRGD YGNNISPERP SFRQPFKDQT 300
SNLRNSSLLN ERAYQEDETF LPHHGPSMDL LNEQRANLKS LLNLLNETLE 350
KNTSERASDL HMISLFNLNK LMLGDPKKNN SERDKRTEKL KKILLDSLAE 400
PFFEHYNFIG DNPIADTDEL KEEIDEFTGS GDTTAITDIR PQQDYGRILR 450
TFTSTKNSAP QAIFTCSQED PWQFRAANDL ACLVFGISQN AIRALTLMDL 500
IHTDSRNFVL HKLLSTEGQE MVFTGEIIGI VQPETLSSSK VVWASFWAKR 550
KNGLLVCVFE KVPCDYVDVL LNLDDFGAEN IVDKCELLSD GPTLSSSSTL 600
SLPKMASSPT GSKLEYSLER KILEKSYTKP TSTENRNGDE NQLDGDSHSE 650
PSLSSSPVRS KKSVKFANDI KDVKSISQSL AKLMDDVRNG VVFDPDDDLL 700
PMPIKVCNHI NETRYFTLNH LSYNIPCAVS STVLEDELKL KIHSLPYQAG 750
LFIVDSHTLD IVSSNKSILK NMFGYHFAEL VGKSITEIIP SFPKFLQFIN 800
DKYPALDITL HKNKGLVLTE HFFRKIQAEI MGDRKSFYTS VGIDGLHRDG 850
CEIKIDFQLR VMNSKVILLW VTHSRDVVFE EYNTNPSQLK MLKESELSLM 900
SSASSSASSS KKSSSRISTG TLKDMSNLST YEDLAHRTNK LKYEIGDDSR 950
AHSQSTLSEQ EQVPLENDKD SGEMMLADPE MKHKLELARI YSRDKSQFVK 1000
EGNFKVDENL IISKISLSPS TESLADSKSS GKGLSPLEEE KLIDENATEN 1050
GLAGSPKDED GIIMTNKRGN QPVSTFLRTP EKNIGAQKHV KKFSDFVSLQ 1100
KMGEGAYGKV NLCIHKKNRY IVVIKMIFKE RILVDTWVRD RKLGTIPSEI 1150
QIMATLNKKP HENILRLLDF FEDDDYYYIE TPVHGETGCI DLFDLIEFKT 1200
NMTEFEAKLI FKQVVAGIKH LHDQGIVHRD IKDENVIVDS KGFVKIIDFG 1250
SAAYVKSGPF DVFVGTIDYA APEVLGGNPY EGQPQDIWAI GILLYTVVFK 1300
ENPFYNIDEI LEGDLKFNNA EEVSEDCIEL IKSILNRCVP KRPTIDDINN 1350
DKWLVI 1356
Length:1,356
Mass (Da):152,332
Last modified:July 27, 2011 - v2
Checksum:i9A2AF4A9C1785586
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731E → Q no nucleotide entry 1 Publication
Sequence conflicti73 – 731E → Q no nucleotide entry 1 Publication
Sequence conflicti73 – 731E → Q no nucleotide entry 1 Publication
Sequence conflicti73 – 731E → Q in AAC04940. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05146 Genomic DNA. Translation: AAC04940.1.
BK006935 Genomic DNA. Translation: DAA06971.2.
PIRiS33653.
RefSeqiNP_009385.2. NM_001178162.2.

Genome annotation databases

EnsemblFungiiYAL017W; YAL017W; YAL017W.
GeneIDi851216.
KEGGisce:YAL017W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L05146 Genomic DNA. Translation: AAC04940.1 .
BK006935 Genomic DNA. Translation: DAA06971.2 .
PIRi S33653.
RefSeqi NP_009385.2. NM_001178162.2.

3D structure databases

ProteinModelPortali P31374.
SMRi P31374. Positions 1055-1354.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31749. 77 interactions.
DIPi DIP-6267N.
IntActi P31374. 16 interactions.
MINTi MINT-604653.
STRINGi 4932.YAL017W.

Proteomic databases

MaxQBi P31374.
PaxDbi P31374.
PeptideAtlasi P31374.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL017W ; YAL017W ; YAL017W .
GeneIDi 851216.
KEGGi sce:YAL017W.

Organism-specific databases

CYGDi YAL017w.
SGDi S000000015. PSK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117599.
KOi K08286.
OMAi STHAYSY.
OrthoDBi EOG7G4QPK.

Enzyme and pathway databases

BioCyci YEAST:G3O-28829-MONOMER.

Miscellaneous databases

NextBioi 968103.

Gene expression databases

Genevestigatori P31374.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00091. PAS. 2 hits.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
    Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
    Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I."
    Clark M.W., Zhong W.-W., Keng T., Storms R.K., Barton A.B., Kaback D.B., Bussey H.
    Yeast 8:133-145(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. "The YAL017 gene on the left arm of chromosome I of Saccharomyces cerevisiae encodes a putative serine/threonine protein kinase."
    Clark M.W., Zhong W.W., Keng T., Storms R.K., Ouellette B.F.F., Barton A., Kaback D.B., Bussey H.
    Yeast 9:543-549(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 73.
    Strain: ATCC 204508 / S288c.
  6. "PAS kinase: an evolutionarily conserved PAS domain-regulated serine/threonine kinase."
    Rutter J., Michnoff C.H., Harper S.M., Gardner K.H., McKnight S.L.
    Proc. Natl. Acad. Sci. U.S.A. 98:8991-8996(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  7. "Coordinate regulation of sugar flux and translation by PAS kinase."
    Rutter J., Probst B.L., McKnight S.L.
    Cell 111:17-28(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1018, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286 AND SER-1035, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "Regulation of glucose partitioning by PAS kinase and Ugp1 phosphorylation."
    Smith T.L., Rutter J.
    Mol. Cell 26:491-499(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1079, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-192; SER-327; SER-926; SER-1035 AND SER-1055, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; SER-255; SER-1023 AND SER-1055, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSK1_YEAST
AccessioniPrimary (citable) accession number: P31374
Secondary accession number(s): D6VPK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 27, 2011
Last modified: May 14, 2014
This is version 139 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 967 molecules/cell in log phase SD medium.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3

Similar proteinsi