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P31374

- PSK1_YEAST

UniProt

P31374 - PSK1_YEAST

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Protein

Serine/threonine-protein kinase PSK1

Gene

PSK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the control of sugar metabolism and translation. Phosphorylates UGP1, which is required for normal glycogen and beta-(1,6)-glucan synthesis. This phosphorylation shifts glucose partitioning toward cell wall glucan synthesis at the expense of glycogen synthesis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei1125 – 11251ATPPROSITE-ProRule annotation
Active sitei1230 – 12301Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi1102 – 11109ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. protein serine/threonine kinase activity Source: SGD
  3. signal transducer activity Source: InterPro

GO - Biological processi

  1. negative regulation of glycogen biosynthetic process Source: SGD
  2. protein phosphorylation Source: SGD
  3. regulation of (1->6)-beta-D-glucan biosynthetic process Source: SGD
  4. regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28829-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PSK1 (EC:2.7.11.1)
Alternative name(s):
PAS kinase 1
Gene namesi
Name:PSK1
Ordered Locus Names:YAL017W
ORF Names:FUN31, YAL002
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
ProteomesiUP000002311: Chromosome I

Organism-specific databases

CYGDiYAL017w.
SGDiS000000015. PSK1.

Subcellular locationi

Cytoplasm 1 Publication

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 13561356Serine/threonine-protein kinase PSK1PRO_0000086042Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei10 – 101Phosphoserine1 Publication
Modified residuei192 – 1921Phosphoserine1 Publication
Modified residuei202 – 2021Phosphoserine1 Publication
Modified residuei255 – 2551Phosphoserine1 Publication
Modified residuei286 – 2861Phosphoserine1 Publication
Modified residuei327 – 3271Phosphoserine1 Publication
Modified residuei926 – 9261Phosphoserine1 Publication
Modified residuei1018 – 10181Phosphoserine1 Publication
Modified residuei1023 – 10231Phosphoserine1 Publication
Modified residuei1035 – 10351Phosphoserine2 Publications
Modified residuei1055 – 10551Phosphoserine2 Publications
Modified residuei1079 – 10791Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31374.
PaxDbiP31374.
PeptideAtlasiP31374.

Expressioni

Gene expression databases

GenevestigatoriP31374.

Interactioni

Protein-protein interaction databases

BioGridi31749. 77 interactions.
DIPiDIP-6267N.
IntActiP31374. 16 interactions.
MINTiMINT-604653.
STRINGi4932.YAL017W.

Structurei

3D structure databases

ProteinModelPortaliP31374.
SMRiP31374. Positions 1068-1354.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini450 – 51869PAS 1Add
BLAST
Domaini738 – 80770PAS 2Add
BLAST
Domaini1096 – 1354259Protein kinasePROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00760000119045.
InParanoidiP31374.
KOiK08286.
OMAiSTHAYSY.
OrthoDBiEOG7G4QPK.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00091. PAS. 2 hits.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31374-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MPYIGASNLS EHSFVNLKEK HAITHKGTSS SVASLQTPPS PDQENHIDNE
60 70 80 90 100
LENYDTSLSD VSTPNKKEGD EFEQSLRDTF ASFRKTKPPP PLDFEQPRLP
110 120 130 140 150
STASSSVDST VSSPLTDEDI KELEFLPNES THSYSYNPLS PNSLAVRLRI
160 170 180 190 200
LKRSLEIIIQ NPSMLLEPTP DDLPPLKEFA GRRSSLPRTS ASANHLMNRN
210 220 230 240 250
KSQIWNTTSA TLNAFVNNTS SSSAASSALS NKKPGTPVFP NLDPTHSQTF
260 270 280 290 300
HRANSLAYLP SILPEQDPLL KHNNSLFRGD YGNNISPERP SFRQPFKDQT
310 320 330 340 350
SNLRNSSLLN ERAYQEDETF LPHHGPSMDL LNEQRANLKS LLNLLNETLE
360 370 380 390 400
KNTSERASDL HMISLFNLNK LMLGDPKKNN SERDKRTEKL KKILLDSLAE
410 420 430 440 450
PFFEHYNFIG DNPIADTDEL KEEIDEFTGS GDTTAITDIR PQQDYGRILR
460 470 480 490 500
TFTSTKNSAP QAIFTCSQED PWQFRAANDL ACLVFGISQN AIRALTLMDL
510 520 530 540 550
IHTDSRNFVL HKLLSTEGQE MVFTGEIIGI VQPETLSSSK VVWASFWAKR
560 570 580 590 600
KNGLLVCVFE KVPCDYVDVL LNLDDFGAEN IVDKCELLSD GPTLSSSSTL
610 620 630 640 650
SLPKMASSPT GSKLEYSLER KILEKSYTKP TSTENRNGDE NQLDGDSHSE
660 670 680 690 700
PSLSSSPVRS KKSVKFANDI KDVKSISQSL AKLMDDVRNG VVFDPDDDLL
710 720 730 740 750
PMPIKVCNHI NETRYFTLNH LSYNIPCAVS STVLEDELKL KIHSLPYQAG
760 770 780 790 800
LFIVDSHTLD IVSSNKSILK NMFGYHFAEL VGKSITEIIP SFPKFLQFIN
810 820 830 840 850
DKYPALDITL HKNKGLVLTE HFFRKIQAEI MGDRKSFYTS VGIDGLHRDG
860 870 880 890 900
CEIKIDFQLR VMNSKVILLW VTHSRDVVFE EYNTNPSQLK MLKESELSLM
910 920 930 940 950
SSASSSASSS KKSSSRISTG TLKDMSNLST YEDLAHRTNK LKYEIGDDSR
960 970 980 990 1000
AHSQSTLSEQ EQVPLENDKD SGEMMLADPE MKHKLELARI YSRDKSQFVK
1010 1020 1030 1040 1050
EGNFKVDENL IISKISLSPS TESLADSKSS GKGLSPLEEE KLIDENATEN
1060 1070 1080 1090 1100
GLAGSPKDED GIIMTNKRGN QPVSTFLRTP EKNIGAQKHV KKFSDFVSLQ
1110 1120 1130 1140 1150
KMGEGAYGKV NLCIHKKNRY IVVIKMIFKE RILVDTWVRD RKLGTIPSEI
1160 1170 1180 1190 1200
QIMATLNKKP HENILRLLDF FEDDDYYYIE TPVHGETGCI DLFDLIEFKT
1210 1220 1230 1240 1250
NMTEFEAKLI FKQVVAGIKH LHDQGIVHRD IKDENVIVDS KGFVKIIDFG
1260 1270 1280 1290 1300
SAAYVKSGPF DVFVGTIDYA APEVLGGNPY EGQPQDIWAI GILLYTVVFK
1310 1320 1330 1340 1350
ENPFYNIDEI LEGDLKFNNA EEVSEDCIEL IKSILNRCVP KRPTIDDINN

DKWLVI
Length:1,356
Mass (Da):152,332
Last modified:July 27, 2011 - v2
Checksum:i9A2AF4A9C1785586
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti73 – 731E → Q no nucleotide entry (PubMed:8458570)Curated
Sequence conflicti73 – 731E → Q no nucleotide entry (PubMed:1561836)Curated
Sequence conflicti73 – 731E → Q no nucleotide entry (PubMed:8322517)Curated
Sequence conflicti73 – 731E → Q in AAC04940. (PubMed:7731988)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04940.1.
BK006935 Genomic DNA. Translation: DAA06971.2.
PIRiS33653.
RefSeqiNP_009385.2. NM_001178162.2.

Genome annotation databases

EnsemblFungiiYAL017W; YAL017W; YAL017W.
GeneIDi851216.
KEGGisce:YAL017W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04940.1 .
BK006935 Genomic DNA. Translation: DAA06971.2 .
PIRi S33653.
RefSeqi NP_009385.2. NM_001178162.2.

3D structure databases

ProteinModelPortali P31374.
SMRi P31374. Positions 1068-1354.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 31749. 77 interactions.
DIPi DIP-6267N.
IntActi P31374. 16 interactions.
MINTi MINT-604653.
STRINGi 4932.YAL017W.

Proteomic databases

MaxQBi P31374.
PaxDbi P31374.
PeptideAtlasi P31374.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblFungii YAL017W ; YAL017W ; YAL017W .
GeneIDi 851216.
KEGGi sce:YAL017W.

Organism-specific databases

CYGDi YAL017w.
SGDi S000000015. PSK1.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00760000119045.
InParanoidi P31374.
KOi K08286.
OMAi STHAYSY.
OrthoDBi EOG7G4QPK.

Enzyme and pathway databases

BioCyci YEAST:G3O-28829-MONOMER.

Miscellaneous databases

NextBioi 968103.

Gene expression databases

Genevestigatori P31374.

Family and domain databases

InterProi IPR011009. Kinase-like_dom.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
SMARTi SM00091. PAS. 2 hits.
[Graphical view ]
SUPFAMi SSF55785. SSF55785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEi PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
    Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
    Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  2. "Identification of a Saccharomyces cerevisiae homolog of the SNF2 transcriptional regulator in the DNA sequence of an 8.6 kb region in the LTE1-CYS1 interval on the left arm of chromosome I."
    Clark M.W., Zhong W.-W., Keng T., Storms R.K., Barton A.B., Kaback D.B., Bussey H.
    Yeast 8:133-145(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: ATCC 204511 / S288c / AB972.
  3. "The YAL017 gene on the left arm of chromosome I of Saccharomyces cerevisiae encodes a putative serine/threonine protein kinase."
    Clark M.W., Zhong W.W., Keng T., Storms R.K., Ouellette B.F.F., Barton A., Kaback D.B., Bussey H.
    Yeast 9:543-549(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION.
    Strain: ATCC 204511 / S288c / AB972.
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC 204508 / S288c.
  5. Cited for: GENOME REANNOTATION, SEQUENCE REVISION TO 73.
    Strain: ATCC 204508 / S288c.
  6. "PAS kinase: an evolutionarily conserved PAS domain-regulated serine/threonine kinase."
    Rutter J., Michnoff C.H., Harper S.M., Gardner K.H., McKnight S.L.
    Proc. Natl. Acad. Sci. U.S.A. 98:8991-8996(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DOMAIN.
  7. "Coordinate regulation of sugar flux and translation by PAS kinase."
    Rutter J., Probst B.L., McKnight S.L.
    Cell 111:17-28(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  8. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
  10. "Quantitative phosphoproteomics applied to the yeast pheromone signaling pathway."
    Gruhler A., Olsen J.V., Mohammed S., Mortensen P., Faergeman N.J., Mann M., Jensen O.N.
    Mol. Cell. Proteomics 4:310-327(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-1018, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: YAL6B.
  11. "Large-scale phosphorylation analysis of alpha-factor-arrested Saccharomyces cerevisiae."
    Li X., Gerber S.A., Rudner A.D., Beausoleil S.A., Haas W., Villen J., Elias J.E., Gygi S.P.
    J. Proteome Res. 6:1190-1197(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-286 AND SER-1035, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Strain: ADR376.
  12. "Regulation of glucose partitioning by PAS kinase and Ugp1 phosphorylation."
    Smith T.L., Rutter J.
    Mol. Cell 26:491-499(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  13. "Analysis of phosphorylation sites on proteins from Saccharomyces cerevisiae by electron transfer dissociation (ETD) mass spectrometry."
    Chi A., Huttenhower C., Geer L.Y., Coon J.J., Syka J.E.P., Bai D.L., Shabanowitz J., Burke D.J., Troyanskaya O.G., Hunt D.F.
    Proc. Natl. Acad. Sci. U.S.A. 104:2193-2198(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-1079, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  14. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
    Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
    Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-10; SER-192; SER-327; SER-926; SER-1035 AND SER-1055, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  15. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
    Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
    Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-202; SER-255; SER-1023 AND SER-1055, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiPSK1_YEAST
AccessioniPrimary (citable) accession number: P31374
Secondary accession number(s): D6VPK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 27, 2011
Last modified: November 26, 2014
This is version 142 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 967 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

External Data

Dasty 3