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Protein

Serine/threonine-protein kinase PSK1

Gene

PSK1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Serine/threonine-protein kinase involved in the control of sugar metabolism and translation. Phosphorylates UGP1, which is required for normal glycogen and beta-(1,6)-glucan synthesis. This phosphorylation shifts glucose partitioning toward cell wall glucan synthesis at the expense of glycogen synthesis.3 Publications

Catalytic activityi

ATP + a protein = ADP + a phosphoprotein.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei1125ATPPROSITE-ProRule annotation1
Active sitei1230Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi1102 – 1110ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • protein serine/threonine kinase activity Source: SGD

GO - Biological processi

  • negative regulation of glycogen biosynthetic process Source: SGD
  • protein phosphorylation Source: SGD
  • regulation of (1->6)-beta-D-glucan biosynthetic process Source: SGD
  • regulation of glucose metabolic process Source: GO_Central
  • regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Translation regulation

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciYEAST:G3O-28829-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Serine/threonine-protein kinase PSK1 (EC:2.7.11.1)
Alternative name(s):
PAS kinase 1
Gene namesi
Name:PSK1
Ordered Locus Names:YAL017W
ORF Names:FUN31, YAL002
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL017W.
SGDiS000000015. PSK1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000860421 – 1356Serine/threonine-protein kinase PSK1Add BLAST1356

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei10PhosphoserineCombined sources1
Modified residuei192PhosphoserineCombined sources1
Modified residuei202PhosphoserineCombined sources1
Modified residuei255PhosphoserineCombined sources1
Modified residuei286PhosphoserineCombined sources1
Modified residuei327PhosphoserineCombined sources1
Modified residuei926PhosphoserineCombined sources1
Modified residuei1018PhosphoserineCombined sources1
Modified residuei1023PhosphoserineCombined sources1
Modified residuei1035PhosphoserineCombined sources1
Modified residuei1055PhosphoserineCombined sources1
Modified residuei1079PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31374.
PRIDEiP31374.

PTM databases

iPTMnetiP31374.

Interactioni

Protein-protein interaction databases

BioGridi31749. 78 interactors.
DIPiDIP-6267N.
IntActiP31374. 16 interactors.
MINTiMINT-604653.

Structurei

3D structure databases

ProteinModelPortaliP31374.
SMRiP31374.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini450 – 518PAS 1Add BLAST69
Domaini738 – 807PAS 2Add BLAST70
Domaini1096 – 1354Protein kinasePROSITE-ProRule annotationAdd BLAST259

Sequence similaritiesi

Belongs to the protein kinase superfamily. Ser/Thr protein kinase family.PROSITE-ProRule annotation
Contains 2 PAS (PER-ARNT-SIM) domains.Curated
Contains 1 protein kinase domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

GeneTreeiENSGT00760000119045.
InParanoidiP31374.
KOiK08286.
OMAiAKLIFKQ.
OrthoDBiEOG092C1BDW.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00091. PAS. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31374-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPYIGASNLS EHSFVNLKEK HAITHKGTSS SVASLQTPPS PDQENHIDNE
60 70 80 90 100
LENYDTSLSD VSTPNKKEGD EFEQSLRDTF ASFRKTKPPP PLDFEQPRLP
110 120 130 140 150
STASSSVDST VSSPLTDEDI KELEFLPNES THSYSYNPLS PNSLAVRLRI
160 170 180 190 200
LKRSLEIIIQ NPSMLLEPTP DDLPPLKEFA GRRSSLPRTS ASANHLMNRN
210 220 230 240 250
KSQIWNTTSA TLNAFVNNTS SSSAASSALS NKKPGTPVFP NLDPTHSQTF
260 270 280 290 300
HRANSLAYLP SILPEQDPLL KHNNSLFRGD YGNNISPERP SFRQPFKDQT
310 320 330 340 350
SNLRNSSLLN ERAYQEDETF LPHHGPSMDL LNEQRANLKS LLNLLNETLE
360 370 380 390 400
KNTSERASDL HMISLFNLNK LMLGDPKKNN SERDKRTEKL KKILLDSLAE
410 420 430 440 450
PFFEHYNFIG DNPIADTDEL KEEIDEFTGS GDTTAITDIR PQQDYGRILR
460 470 480 490 500
TFTSTKNSAP QAIFTCSQED PWQFRAANDL ACLVFGISQN AIRALTLMDL
510 520 530 540 550
IHTDSRNFVL HKLLSTEGQE MVFTGEIIGI VQPETLSSSK VVWASFWAKR
560 570 580 590 600
KNGLLVCVFE KVPCDYVDVL LNLDDFGAEN IVDKCELLSD GPTLSSSSTL
610 620 630 640 650
SLPKMASSPT GSKLEYSLER KILEKSYTKP TSTENRNGDE NQLDGDSHSE
660 670 680 690 700
PSLSSSPVRS KKSVKFANDI KDVKSISQSL AKLMDDVRNG VVFDPDDDLL
710 720 730 740 750
PMPIKVCNHI NETRYFTLNH LSYNIPCAVS STVLEDELKL KIHSLPYQAG
760 770 780 790 800
LFIVDSHTLD IVSSNKSILK NMFGYHFAEL VGKSITEIIP SFPKFLQFIN
810 820 830 840 850
DKYPALDITL HKNKGLVLTE HFFRKIQAEI MGDRKSFYTS VGIDGLHRDG
860 870 880 890 900
CEIKIDFQLR VMNSKVILLW VTHSRDVVFE EYNTNPSQLK MLKESELSLM
910 920 930 940 950
SSASSSASSS KKSSSRISTG TLKDMSNLST YEDLAHRTNK LKYEIGDDSR
960 970 980 990 1000
AHSQSTLSEQ EQVPLENDKD SGEMMLADPE MKHKLELARI YSRDKSQFVK
1010 1020 1030 1040 1050
EGNFKVDENL IISKISLSPS TESLADSKSS GKGLSPLEEE KLIDENATEN
1060 1070 1080 1090 1100
GLAGSPKDED GIIMTNKRGN QPVSTFLRTP EKNIGAQKHV KKFSDFVSLQ
1110 1120 1130 1140 1150
KMGEGAYGKV NLCIHKKNRY IVVIKMIFKE RILVDTWVRD RKLGTIPSEI
1160 1170 1180 1190 1200
QIMATLNKKP HENILRLLDF FEDDDYYYIE TPVHGETGCI DLFDLIEFKT
1210 1220 1230 1240 1250
NMTEFEAKLI FKQVVAGIKH LHDQGIVHRD IKDENVIVDS KGFVKIIDFG
1260 1270 1280 1290 1300
SAAYVKSGPF DVFVGTIDYA APEVLGGNPY EGQPQDIWAI GILLYTVVFK
1310 1320 1330 1340 1350
ENPFYNIDEI LEGDLKFNNA EEVSEDCIEL IKSILNRCVP KRPTIDDINN

DKWLVI
Length:1,356
Mass (Da):152,332
Last modified:July 27, 2011 - v2
Checksum:i9A2AF4A9C1785586
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti73E → Q no nucleotide entry (PubMed:8458570).Curated1
Sequence conflicti73E → Q no nucleotide entry (PubMed:1561836).Curated1
Sequence conflicti73E → Q no nucleotide entry (PubMed:8322517).Curated1
Sequence conflicti73E → Q in AAC04940 (PubMed:7731988).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04940.1.
BK006935 Genomic DNA. Translation: DAA06971.2.
PIRiS33653.
RefSeqiNP_009385.2. NM_001178162.2.

Genome annotation databases

EnsemblFungiiYAL017W; YAL017W; YAL017W.
GeneIDi851216.
KEGGisce:YAL017W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04940.1.
BK006935 Genomic DNA. Translation: DAA06971.2.
PIRiS33653.
RefSeqiNP_009385.2. NM_001178162.2.

3D structure databases

ProteinModelPortaliP31374.
SMRiP31374.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31749. 78 interactors.
DIPiDIP-6267N.
IntActiP31374. 16 interactors.
MINTiMINT-604653.

PTM databases

iPTMnetiP31374.

Proteomic databases

MaxQBiP31374.
PRIDEiP31374.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL017W; YAL017W; YAL017W.
GeneIDi851216.
KEGGisce:YAL017W.

Organism-specific databases

EuPathDBiFungiDB:YAL017W.
SGDiS000000015. PSK1.

Phylogenomic databases

GeneTreeiENSGT00760000119045.
InParanoidiP31374.
KOiK08286.
OMAiAKLIFKQ.
OrthoDBiEOG092C1BDW.

Enzyme and pathway databases

BioCyciYEAST:G3O-28829-MONOMER.

Miscellaneous databases

PROiP31374.

Family and domain databases

InterProiIPR011009. Kinase-like_dom.
IPR000014. PAS.
IPR000719. Prot_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
SMARTiSM00091. PAS. 2 hits.
SM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF55785. SSF55785. 1 hit.
SSF56112. SSF56112. 1 hit.
PROSITEiPS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPSK1_YEAST
AccessioniPrimary (citable) accession number: P31374
Secondary accession number(s): D6VPK1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 27, 2011
Last modified: November 2, 2016
This is version 158 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 967 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.