Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P31373

- CYS3_YEAST

UniProt

P31373 - CYS3_YEAST

Protein

Cystathionine gamma-lyase

Gene

CYS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 141 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.

    Cofactori

    Pyridoxal phosphate.1 Publication

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei52 – 521SubstrateBy similarity
    Binding sitei104 – 1041SubstrateBy similarity
    Binding sitei109 – 1091SubstrateBy similarity
    Binding sitei334 – 3341SubstrateBy similarity

    GO - Molecular functioni

    1. cystathionine gamma-lyase activity Source: SGD
    2. identical protein binding Source: IntAct
    3. L-cysteine desulfhydrase activity Source: UniProtKB-EC
    4. L-cystine L-cysteine-lyase (deaminating) Source: UniProtKB-EC
    5. pyridoxal phosphate binding Source: InterPro

    GO - Biological processi

    1. cysteine biosynthetic process Source: SGD
    2. cysteine biosynthetic process via cystathionine Source: SGD
    3. transsulfuration Source: SGD

    Keywords - Molecular functioni

    Lyase

    Keywords - Biological processi

    Amino-acid biosynthesis, Cysteine biosynthesis

    Keywords - Ligandi

    Pyridoxal phosphate

    Enzyme and pathway databases

    BioCyciMetaCyc:MONOMER-389.
    YEAST:YAL012W-MONOMER.
    ReactomeiREACT_188977. Cysteine formation from homocysteine.
    REACT_188980. Degradation of cysteine and homocysteine.
    UniPathwayiUPA00136; UER00202.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Cystathionine gamma-lyase (EC:4.4.1.1)
    Alternative name(s):
    Gamma-cystathionase
    Sulfur transfer protein 1
    Gene namesi
    Name:CYS3
    Synonyms:CYI1, STR1
    Ordered Locus Names:YAL012W
    ORF Names:FUN35
    OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
    Taxonomic identifieri559292 [NCBI]
    Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
    ProteomesiUP000002311: Chromosome I

    Organism-specific databases

    SGDiS000000010. CYS3.

    Subcellular locationi

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11Removed1 Publication
    Chaini2 – 394393Cystathionine gamma-lyasePRO_0000114754Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei204 – 2041N6-(pyridoxal phosphate)lysineBy similarity
    Modified residuei362 – 3621Phosphoserine2 Publications

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiP31373.
    PaxDbiP31373.
    PeptideAtlasiP31373.

    Expressioni

    Gene expression databases

    GenevestigatoriP31373.

    Interactioni

    Subunit structurei

    Homotetramer.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    itself2EBI-5473,EBI-5473

    Protein-protein interaction databases

    BioGridi31754. 155 interactions.
    DIPiDIP-4440N.
    IntActiP31373. 5 interactions.
    MINTiMINT-483065.
    STRINGi4932.YAL012W.

    Structurei

    Secondary structure

    1
    394
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi9 – 157
    Beta strandi38 – 4710
    Turni51 – 533
    Helixi56 – 6813
    Beta strandi72 – 787
    Helixi80 – 8910
    Beta strandi96 – 1027
    Helixi105 – 1139
    Turni116 – 1183
    Beta strandi123 – 1275
    Helixi128 – 1358
    Beta strandi138 – 1458
    Turni150 – 1523
    Helixi158 – 16811
    Turni169 – 1735
    Beta strandi175 – 1795
    Turni181 – 1833
    Helixi184 – 1874
    Helixi190 – 1934
    Beta strandi196 – 2016
    Turni202 – 2076
    Beta strandi215 – 2206
    Helixi222 – 23514
    Helixi241 – 25111
    Helixi254 – 27219
    Turni276 – 2783
    Beta strandi279 – 2835
    Helixi293 – 2997
    Helixi301 – 3033
    Beta strandi307 – 3159
    Helixi317 – 32610
    Beta strandi328 – 3325
    Beta strandi342 – 3443
    Turni346 – 3505
    Turni356 – 3605
    Beta strandi361 – 3633
    Beta strandi368 – 3725
    Helixi378 – 39316

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1N8PX-ray2.60A/B/C/D2-394[»]
    ProteinModelPortaliP31373.
    SMRiP31373. Positions 2-394.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP31373.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the trans-sulfuration enzymes family.Curated

    Phylogenomic databases

    eggNOGiCOG0626.
    GeneTreeiENSGT00390000000312.
    HOGENOMiHOG000246415.
    KOiK01758.
    OMAiHERNANE.
    OrthoDBiEOG71VT31.

    Family and domain databases

    Gene3Di3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view]
    PANTHERiPTHR11808. PTHR11808. 1 hit.
    PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001434. CGS. 1 hit.
    SUPFAMiSSF53383. SSF53383. 1 hit.
    PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31373-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLQESDKFA TKAIHAGEHV DVHGSVIEPI SLSTTFKQSS PANPIGTYEY    50
    SRSQNPNREN LERAVAALEN AQYGLAFSSG SATTATILQS LPQGSHAVSI 100
    GDVYGGTHRY FTKVANAHGV ETSFTNDLLN DLPQLIKENT KLVWIETPTN 150
    PTLKVTDIQK VADLIKKHAA GQDVILVVDN TFLSPYISNP LNFGADIVVH 200
    SATKYINGHS DVVLGVLATN NKPLYERLQF LQNAIGAIPS PFDAWLTHRG 250
    LKTLHLRVRQ AALSANKIAE FLAADKENVV AVNYPGLKTH PNYDVVLKQH 300
    RDALGGGMIS FRIKGGAEAA SKFASSTRLF TLAESLGGIE SLLEVPAVMT 350
    HGGIPKEARE ASGVFDDLVR ISVGIEDTDD LLEDIKQALK QATN 394
    Length:394
    Mass (Da):42,542
    Last modified:January 23, 2007 - v2
    Checksum:i861A5AA7557687FC
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05146 Genomic DNA. Translation: AAC04945.1.
    D14135 Genomic DNA. Translation: BAA03190.1.
    BK006935 Genomic DNA. Translation: DAA06976.1.
    PIRiS31228.
    RefSeqiNP_009390.1. NM_001178157.1.

    Genome annotation databases

    EnsemblFungiiYAL012W; YAL012W; YAL012W.
    GeneIDi851221.
    KEGGisce:YAL012W.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L05146 Genomic DNA. Translation: AAC04945.1 .
    D14135 Genomic DNA. Translation: BAA03190.1 .
    BK006935 Genomic DNA. Translation: DAA06976.1 .
    PIRi S31228.
    RefSeqi NP_009390.1. NM_001178157.1.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1N8P X-ray 2.60 A/B/C/D 2-394 [» ]
    ProteinModelPortali P31373.
    SMRi P31373. Positions 2-394.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 31754. 155 interactions.
    DIPi DIP-4440N.
    IntActi P31373. 5 interactions.
    MINTi MINT-483065.
    STRINGi 4932.YAL012W.

    Proteomic databases

    MaxQBi P31373.
    PaxDbi P31373.
    PeptideAtlasi P31373.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblFungii YAL012W ; YAL012W ; YAL012W .
    GeneIDi 851221.
    KEGGi sce:YAL012W.

    Organism-specific databases

    SGDi S000000010. CYS3.

    Phylogenomic databases

    eggNOGi COG0626.
    GeneTreei ENSGT00390000000312.
    HOGENOMi HOG000246415.
    KOi K01758.
    OMAi HERNANE.
    OrthoDBi EOG71VT31.

    Enzyme and pathway databases

    UniPathwayi UPA00136 ; UER00202 .
    BioCyci MetaCyc:MONOMER-389.
    YEAST:YAL012W-MONOMER.
    Reactomei REACT_188977. Cysteine formation from homocysteine.
    REACT_188980. Degradation of cysteine and homocysteine.

    Miscellaneous databases

    EvolutionaryTracei P31373.
    NextBioi 968118.
    PROi P31373.

    Gene expression databases

    Genevestigatori P31373.

    Family and domain databases

    Gene3Di 3.40.640.10. 1 hit.
    3.90.1150.10. 1 hit.
    InterProi IPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
    IPR015424. PyrdxlP-dep_Trfase.
    IPR015421. PyrdxlP-dep_Trfase_major_sub1.
    IPR015422. PyrdxlP-dep_Trfase_major_sub2.
    [Graphical view ]
    PANTHERi PTHR11808. PTHR11808. 1 hit.
    Pfami PF01053. Cys_Met_Meta_PP. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001434. CGS. 1 hit.
    SUPFAMi SSF53383. SSF53383. 1 hit.
    PROSITEi PS00868. CYS_MET_METAB_PP. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning and characterization of the CYS3 (CYI1) gene of Saccharomyces cerevisiae."
      Ono B., Tanaka K., Naito K., Heike C., Shinoda S., Yamamoto S., Ohmori S., Oshima T., Toh-e A.
      J. Bacteriol. 174:3339-3347(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PARTIAL PROTEIN SEQUENCE.
    2. "Cloning and bacterial expression of the CYS3 gene encoding cystathionine gamma-lyase of Saccharomyces cerevisiae and the physicochemical and enzymatic properties of the protein."
      Yamagata S., D'Andrea R.J., Fujisaki S., Isaji M., Nakamura K.
      J. Bacteriol. 175:4800-4808(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], CHARACTERIZATION.
      Strain: DBY939.
    3. "Physical localization of yeast CYS3, a gene whose product resembles the rat gamma-cystathionase and Escherichia coli cystathionine gamma-synthase enzymes."
      Barton A.B., Kaback D.B., Clark M.W., Keng T., Ouellette B.F.F., Storms R.K., Zeng B., Zhong W.W., Fortin N., Delaney S., Bussey H.
      Yeast 9:363-369(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    4. "Sequencing of chromosome I from Saccharomyces cerevisiae: analysis of a 32 kb region between the LTE1 and SPO7 genes."
      Ouellette B.F.F., Clark M.W., Keng T., Storms R.K., Zhong W.-W., Zeng B., Fortin N., Delaney S., Barton A.B., Kaback D.B., Bussey H.
      Genome 36:32-42(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: ATCC 204511 / S288c / AB972.
    5. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: ATCC 204508 / S288c.
    6. Cited for: GENOME REANNOTATION.
      Strain: ATCC 204508 / S288c.
    7. "Cystathionine gamma-lyase of Saccharomyces cerevisiae: structural gene and cystathionine gamma-synthase activity."
      Ono B., Ishii N., Naito K., Miyoshi S., Shinoda S., Yamamoto S., Ohmori S.
      Yeast 9:389-397(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: PROTEIN SEQUENCE OF 2-19, CHARACTERIZATION.
    8. Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
    9. "A multidimensional chromatography technology for in-depth phosphoproteome analysis."
      Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H.
      Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    10. "Global analysis of Cdk1 substrate phosphorylation sites provides insights into evolution."
      Holt L.J., Tuch B.B., Villen J., Johnson A.D., Gygi S.P., Morgan D.O.
      Science 325:1682-1686(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-362, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    11. "Determinants of enzymatic specificity in the Cys-Met-metabolism PLP-dependent enzymes family: crystal structure of cystathionine gamma-lyase from yeast and intrafamiliar structure comparison."
      Messerschmidt A., Worbs M., Steegborn C., Wahl M.C., Huber R., Laber B., Clausen T.
      Biol. Chem. 384:373-386(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.6 ANGSTROMS) OF 2-393 IN COMPLEX WITH PYRIDOXAL PHOSPHATE, COFACTOR, SUBUNIT.

    Entry informationi

    Entry nameiCYS3_YEAST
    AccessioniPrimary (citable) accession number: P31373
    Secondary accession number(s): D6VPK6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 141 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programFungal Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Present with 38300 molecules/cell in log phase SD medium.1 Publication

    Keywords - Technical termi

    3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families
    4. Yeast
      Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
    5. Yeast chromosome I
      Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

    External Data

    Dasty 3