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Protein

Cystathionine gamma-lyase

Gene

CYS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.

Cofactori

pyridoxal 5'-phosphate1 Publication

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (CYS4)
  2. Cystathionine gamma-lyase (CYS3)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei52 – 521SubstrateBy similarity
Binding sitei104 – 1041SubstrateBy similarity
Binding sitei109 – 1091SubstrateBy similarity
Binding sitei334 – 3341SubstrateBy similarity

GO - Molecular functioni

GO - Biological processi

  • 'de novo' L-methionine biosynthetic process Source: GO_Central
  • cysteine biosynthetic process Source: SGD
  • cysteine biosynthetic process via cystathionine Source: SGD
  • transsulfuration Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Lyase

Keywords - Biological processi

Amino-acid biosynthesis, Cysteine biosynthesis

Keywords - Ligandi

Pyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-389.
YEAST:YAL012W-MONOMER.
BRENDAi4.4.1.1. 984.
ReactomeiR-SCE-1614558. Degradation of cysteine and homocysteine.
R-SCE-1614603. Cysteine formation from homocysteine.
UniPathwayiUPA00136; UER00202.

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-lyase (EC:4.4.1.1)
Alternative name(s):
Gamma-cystathionase
Sulfur transfer protein 1
Gene namesi
Name:CYS3
Synonyms:CYI1, STR1
Ordered Locus Names:YAL012W
ORF Names:FUN35
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL012W.
SGDiS000000010. CYS3.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemoved1 Publication
Chaini2 – 394393Cystathionine gamma-lyasePRO_0000114754Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei204 – 2041N6-(pyridoxal phosphate)lysineBy similarity
Modified residuei362 – 3621PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31373.

PTM databases

iPTMnetiP31373.

Interactioni

Subunit structurei

Homotetramer.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
itself2EBI-5473,EBI-5473

GO - Molecular functioni

  • identical protein binding Source: IntAct

Protein-protein interaction databases

BioGridi31754. 160 interactions.
DIPiDIP-4440N.
IntActiP31373. 5 interactions.
MINTiMINT-483065.

Structurei

Secondary structure

1
394
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi9 – 157Combined sources
Beta strandi38 – 4710Combined sources
Turni51 – 533Combined sources
Helixi56 – 6813Combined sources
Beta strandi72 – 787Combined sources
Helixi80 – 8910Combined sources
Beta strandi96 – 1027Combined sources
Helixi105 – 1139Combined sources
Turni116 – 1183Combined sources
Beta strandi123 – 1275Combined sources
Helixi128 – 1358Combined sources
Beta strandi138 – 1458Combined sources
Turni150 – 1523Combined sources
Helixi158 – 16811Combined sources
Turni169 – 1735Combined sources
Beta strandi175 – 1795Combined sources
Turni181 – 1833Combined sources
Helixi184 – 1874Combined sources
Helixi190 – 1934Combined sources
Beta strandi196 – 2016Combined sources
Turni202 – 2076Combined sources
Beta strandi215 – 2206Combined sources
Helixi222 – 23514Combined sources
Helixi241 – 25111Combined sources
Helixi254 – 27219Combined sources
Turni276 – 2783Combined sources
Beta strandi279 – 2835Combined sources
Helixi293 – 2997Combined sources
Helixi301 – 3033Combined sources
Beta strandi307 – 3159Combined sources
Helixi317 – 32610Combined sources
Beta strandi328 – 3325Combined sources
Beta strandi342 – 3443Combined sources
Turni346 – 3505Combined sources
Turni356 – 3605Combined sources
Beta strandi361 – 3633Combined sources
Beta strandi368 – 3725Combined sources
Helixi378 – 39316Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N8PX-ray2.60A/B/C/D2-394[»]
ProteinModelPortaliP31373.
SMRiP31373. Positions 2-394.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31373.

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000312.
HOGENOMiHOG000246415.
InParanoidiP31373.
KOiK01758.
OMAiYTRTSNP.
OrthoDBiEOG092C2AJE.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQESDKFA TKAIHAGEHV DVHGSVIEPI SLSTTFKQSS PANPIGTYEY
60 70 80 90 100
SRSQNPNREN LERAVAALEN AQYGLAFSSG SATTATILQS LPQGSHAVSI
110 120 130 140 150
GDVYGGTHRY FTKVANAHGV ETSFTNDLLN DLPQLIKENT KLVWIETPTN
160 170 180 190 200
PTLKVTDIQK VADLIKKHAA GQDVILVVDN TFLSPYISNP LNFGADIVVH
210 220 230 240 250
SATKYINGHS DVVLGVLATN NKPLYERLQF LQNAIGAIPS PFDAWLTHRG
260 270 280 290 300
LKTLHLRVRQ AALSANKIAE FLAADKENVV AVNYPGLKTH PNYDVVLKQH
310 320 330 340 350
RDALGGGMIS FRIKGGAEAA SKFASSTRLF TLAESLGGIE SLLEVPAVMT
360 370 380 390
HGGIPKEARE ASGVFDDLVR ISVGIEDTDD LLEDIKQALK QATN
Length:394
Mass (Da):42,542
Last modified:January 23, 2007 - v2
Checksum:i861A5AA7557687FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04945.1.
D14135 Genomic DNA. Translation: BAA03190.1.
BK006935 Genomic DNA. Translation: DAA06976.1.
PIRiS31228.
RefSeqiNP_009390.1. NM_001178157.1.

Genome annotation databases

EnsemblFungiiYAL012W; YAL012W; YAL012W.
GeneIDi851221.
KEGGisce:YAL012W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA. Translation: AAC04945.1.
D14135 Genomic DNA. Translation: BAA03190.1.
BK006935 Genomic DNA. Translation: DAA06976.1.
PIRiS31228.
RefSeqiNP_009390.1. NM_001178157.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1N8PX-ray2.60A/B/C/D2-394[»]
ProteinModelPortaliP31373.
SMRiP31373. Positions 2-394.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31754. 160 interactions.
DIPiDIP-4440N.
IntActiP31373. 5 interactions.
MINTiMINT-483065.

PTM databases

iPTMnetiP31373.

Proteomic databases

MaxQBiP31373.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYAL012W; YAL012W; YAL012W.
GeneIDi851221.
KEGGisce:YAL012W.

Organism-specific databases

EuPathDBiFungiDB:YAL012W.
SGDiS000000010. CYS3.

Phylogenomic databases

GeneTreeiENSGT00390000000312.
HOGENOMiHOG000246415.
InParanoidiP31373.
KOiK01758.
OMAiYTRTSNP.
OrthoDBiEOG092C2AJE.

Enzyme and pathway databases

UniPathwayiUPA00136; UER00202.
BioCyciMetaCyc:MONOMER-389.
YEAST:YAL012W-MONOMER.
BRENDAi4.4.1.1. 984.
ReactomeiR-SCE-1614558. Degradation of cysteine and homocysteine.
R-SCE-1614603. Cysteine formation from homocysteine.

Miscellaneous databases

EvolutionaryTraceiP31373.
PROiP31373.

Family and domain databases

CDDicd00614. CGS_like. 1 hit.
Gene3Di3.40.640.10. 1 hit.
3.90.1150.10. 1 hit.
InterProiIPR000277. Cys/Met-Metab_PyrdxlP-dep_enz.
IPR015424. PyrdxlP-dep_Trfase.
IPR015421. PyrdxlP-dep_Trfase_major_sub1.
IPR015422. PyrdxlP-dep_Trfase_major_sub2.
[Graphical view]
PANTHERiPTHR11808. PTHR11808. 1 hit.
PfamiPF01053. Cys_Met_Meta_PP. 1 hit.
[Graphical view]
PIRSFiPIRSF001434. CGS. 1 hit.
SUPFAMiSSF53383. SSF53383. 1 hit.
PROSITEiPS00868. CYS_MET_METAB_PP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiCYS3_YEAST
AccessioniPrimary (citable) accession number: P31373
Secondary accession number(s): D6VPK6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: September 7, 2016
This is version 159 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 38300 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.