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Protein

Cystathionine gamma-lyase

Gene

CYS3

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Miscellaneous

Present with 38300 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

L-cystathionine + H2O = L-cysteine + NH3 + 2-oxobutanoate.

Cofactori

pyridoxal 5'-phosphate1 Publication

Pathwayi: L-cysteine biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Cystathionine beta-synthase (CYS4)
  2. Cystathionine gamma-lyase (CYS3)
This subpathway is part of the pathway L-cysteine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-cysteine from L-homocysteine and L-serine, the pathway L-cysteine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei52SubstrateBy similarity1
Binding sitei104SubstrateBy similarity1
Binding sitei109SubstrateBy similarity1
Binding sitei334SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

  • 'de novo' L-methionine biosynthetic process Source: GO_Central
  • cysteine biosynthetic process Source: SGD
  • cysteine biosynthetic process via cystathionine Source: SGD
  • transsulfuration Source: SGD

Keywordsi

Molecular functionLyase
Biological processAmino-acid biosynthesis, Cysteine biosynthesis
LigandPyridoxal phosphate

Enzyme and pathway databases

BioCyciMetaCyc:YAL012W-MONOMER
YEAST:YAL012W-MONOMER
BRENDAi4.4.1.1 984
ReactomeiR-SCE-1614558 Degradation of cysteine and homocysteine
R-SCE-1614603 Cysteine formation from homocysteine
UniPathwayiUPA00136; UER00202

Names & Taxonomyi

Protein namesi
Recommended name:
Cystathionine gamma-lyase (EC:4.4.1.1)
Alternative name(s):
Gamma-cystathionase
Sulfur transfer protein 1
Gene namesi
Name:CYS3
Synonyms:CYI1, STR1
Ordered Locus Names:YAL012W
ORF Names:FUN35
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:YAL012W
SGDiS000000010 CYS3

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001147542 – 394Cystathionine gamma-lyaseAdd BLAST393

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei204N6-(pyridoxal phosphate)lysineBy similarity1
Modified residuei362PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31373
PaxDbiP31373
PRIDEiP31373

PTM databases

iPTMnetiP31373

Interactioni

Subunit structurei

Homotetramer.1 Publication

Protein-protein interaction databases

BioGridi31754, 213 interactors
DIPiDIP-4440N
IntActiP31373, 7 interactors
MINTiP31373
STRINGi4932.YAL012W

Structurei

Secondary structure

1394
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi9 – 15Combined sources7
Beta strandi38 – 47Combined sources10
Turni51 – 53Combined sources3
Helixi56 – 68Combined sources13
Beta strandi72 – 78Combined sources7
Helixi80 – 89Combined sources10
Beta strandi96 – 102Combined sources7
Helixi105 – 113Combined sources9
Turni116 – 118Combined sources3
Beta strandi123 – 127Combined sources5
Helixi128 – 135Combined sources8
Beta strandi138 – 145Combined sources8
Turni150 – 152Combined sources3
Helixi158 – 168Combined sources11
Turni169 – 173Combined sources5
Beta strandi175 – 179Combined sources5
Turni181 – 183Combined sources3
Helixi184 – 187Combined sources4
Helixi190 – 193Combined sources4
Beta strandi196 – 201Combined sources6
Turni202 – 207Combined sources6
Beta strandi215 – 220Combined sources6
Helixi222 – 235Combined sources14
Helixi241 – 251Combined sources11
Helixi254 – 272Combined sources19
Turni276 – 278Combined sources3
Beta strandi279 – 283Combined sources5
Helixi293 – 299Combined sources7
Helixi301 – 303Combined sources3
Beta strandi307 – 315Combined sources9
Helixi317 – 326Combined sources10
Beta strandi328 – 332Combined sources5
Beta strandi342 – 344Combined sources3
Turni346 – 350Combined sources5
Turni356 – 360Combined sources5
Beta strandi361 – 363Combined sources3
Beta strandi368 – 372Combined sources5
Helixi378 – 393Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1N8PX-ray2.60A/B/C/D2-394[»]
ProteinModelPortaliP31373
SMRiP31373
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31373

Family & Domainsi

Sequence similaritiesi

Belongs to the trans-sulfuration enzymes family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000000312
HOGENOMiHOG000246415
InParanoidiP31373
KOiK01758
OMAiHPGRMTH
OrthoDBiEOG092C2AJE

Family and domain databases

CDDicd00614 CGS_like, 1 hit
Gene3Di3.40.640.10, 1 hit
3.90.1150.10, 1 hit
InterProiView protein in InterPro
IPR000277 Cys/Met-Metab_PyrdxlP-dep_enz
IPR015424 PyrdxlP-dep_Trfase
IPR015422 PyrdxlP-dep_Trfase_dom1
IPR015421 PyrdxlP-dep_Trfase_major
PANTHERiPTHR11808 PTHR11808, 1 hit
PfamiView protein in Pfam
PF01053 Cys_Met_Meta_PP, 1 hit
PIRSFiPIRSF001434 CGS, 1 hit
SUPFAMiSSF53383 SSF53383, 1 hit
PROSITEiView protein in PROSITE
PS00868 CYS_MET_METAB_PP, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31373-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLQESDKFA TKAIHAGEHV DVHGSVIEPI SLSTTFKQSS PANPIGTYEY
60 70 80 90 100
SRSQNPNREN LERAVAALEN AQYGLAFSSG SATTATILQS LPQGSHAVSI
110 120 130 140 150
GDVYGGTHRY FTKVANAHGV ETSFTNDLLN DLPQLIKENT KLVWIETPTN
160 170 180 190 200
PTLKVTDIQK VADLIKKHAA GQDVILVVDN TFLSPYISNP LNFGADIVVH
210 220 230 240 250
SATKYINGHS DVVLGVLATN NKPLYERLQF LQNAIGAIPS PFDAWLTHRG
260 270 280 290 300
LKTLHLRVRQ AALSANKIAE FLAADKENVV AVNYPGLKTH PNYDVVLKQH
310 320 330 340 350
RDALGGGMIS FRIKGGAEAA SKFASSTRLF TLAESLGGIE SLLEVPAVMT
360 370 380 390
HGGIPKEARE ASGVFDDLVR ISVGIEDTDD LLEDIKQALK QATN
Length:394
Mass (Da):42,542
Last modified:January 23, 2007 - v2
Checksum:i861A5AA7557687FC
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L05146 Genomic DNA Translation: AAC04945.1
D14135 Genomic DNA Translation: BAA03190.1
BK006935 Genomic DNA Translation: DAA06976.1
PIRiS31228
RefSeqiNP_009390.1, NM_001178157.1

Genome annotation databases

EnsemblFungiiYAL012W; YAL012W; YAL012W
GeneIDi851221
KEGGisce:YAL012W

Similar proteinsi

Entry informationi

Entry nameiCYS3_YEAST
AccessioniPrimary (citable) accession number: P31373
Secondary accession number(s): D6VPK6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: May 23, 2018
This is version 175 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome I
    Yeast (Saccharomyces cerevisiae) chromosome I: entries and gene names

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