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P31369

- PDM2A_DROME

UniProt

P31369 - PDM2A_DROME

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Protein

POU domain protein 2, isoform A

Gene

pdm2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

DNA-binding regulatory protein implicated in early development. Involved in neuronal cell fate decision. May act as an octamer-dependent activator of transcription. Could also play an early role in specific ectodermal cells, and a subsequent role in the embryonic nervous system.4 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi391 – 45060HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: UniProtKB
  2. RNA polymerase II core promoter proximal region sequence-specific DNA binding transcription factor activity involved in positive regulation of transcription Source: FlyBase
  3. sequence-specific DNA binding Source: InterPro

GO - Biological processi

  1. central nervous system development Source: UniProtKB
  2. ectoderm development Source: UniProtKB
  3. generation of neurons Source: FlyBase
  4. neuroblast development Source: FlyBase
  5. positive regulation of transcription from RNA polymerase II promoter Source: GOC
  6. regulation of transcription, DNA-templated Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Activator, Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Enzyme and pathway databases

ReactomeiREACT_208061. RNA Polymerase III Abortive And Retractive Initiation.
REACT_217491. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain protein 2, isoform A
Alternative name(s):
Miti-mere
Pdm-2
Protein didymous
dOct2
dPOU-28
Gene namesi
Name:pdm2
Synonyms:dim, OCT2, pdm-2, POU-28
ORF Names:CG12287
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
ProteomesiUP000000803: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0004394. pdm2.

Subcellular locationi

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 498498POU domain protein 2, isoform APRO_0000100778Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei72 – 721Phosphoserine1 Publication
Modified residuei211 – 2111Phosphoserine1 Publication
Modified residuei215 – 2151Phosphoserine1 Publication
Modified residuei217 – 2171Phosphoserine1 Publication
Modified residuei219 – 2191Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP31369.
PRIDEiP31369.

Expressioni

Tissue specificityi

Initial expression in cellular blastoderm stage, then in ectodermal stripes during germband extension. Broad expression in the neuroectoderm followed by limitation to discrete subsets of CNS cells, and expression in specific PNS neurons and support cells.4 Publications

Developmental stagei

Expressed primarily during the first half of embryogenesis.2 Publications

Gene expression databases

BgeeiP31369.
ExpressionAtlasiP31369. differential.

Interactioni

Protein-protein interaction databases

BioGridi60717. 1 interaction.

Structurei

3D structure databases

ProteinModelPortaliP31369.
SMRiP31369. Positions 289-451.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini286 – 36075POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi5 – 106Poly-Gln
Compositional biasi74 – 796Poly-Glu

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG267922.
GeneTreeiENSGT00760000118935.
KOiK09364.
OrthoDBiEOG7DJSMG.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform A (identifier: P31369-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MMVLQQQQQQ RLWDATTTSN TNTQTQQSAN VESTPTKVCH QENAATHTFM
60 70 80 90 100
RHMSNSPTPP SPLRSLSDCG KSFEEEELEL GENCEMPQNL SSKRQARELD
110 120 130 140 150
SELENEVLDL APPPKRLAEE QEEEKVASVN PPQPVAFAPE EMHQALQLQL
160 170 180 190 200
HSYIEMVRQL APEAFPNPNL ATQFLLQNSL QALAQFQALQ QMKQQQREDP
210 220 230 240 250
LPSYSTPLAK SPLRSPSLSP VPRHSKSQQR TPPNSMTANS LGMSSAVMTP
260 270 280 290 300
NTPSMQQQPQ LQQSTPKPTS GLTVASAMAK LEQSPEETTD LEELEQFAKT
310 320 330 340 350
FKQRRIKLGF TQGDVGLAMG KLYGNDFSQT TISRFEALNL SFKNMCKLKP
360 370 380 390 400
LLQKWLEDAD STVAKSGGGV FNINTMTSTL SSTPESILGR RRKKRTSIET
410 420 430 440 450
TVRTTLEKAF LMNCKPTSEE ISQLSERLNM DKEVIRVWFC NRRQKEKRIN
460 470 480 490
PSLDLDSPTG TPLSSHAFGY PPQALNMSHM QMEGGSGSFC GSSISSGE
Length:498
Mass (Da):55,463
Last modified:July 1, 1993 - v1
Checksum:i60F17AF776603974
GO
Isoform B (identifier: Q9VK71-1) [UniParc]FASTAAdd to Basket

The sequence of this isoform can be found in the external entry Q9VK71.
Isoforms of the same protein are often annotated in two different entries if their sequences differ significantly.

Note: No experimental confirmation available.

Length:893
Mass (Da):98,465
GO

Sequence cautioni

The sequence AAQ23557.1 differs from that shown. Reason: Frameshift at position 95. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti221 – 2244VPRH → GAPAR in AAA28481. (PubMed:1881906)Curated
Sequence conflicti248 – 2481M → S in AAA28732. (PubMed:10731132)Curated
Sequence conflicti447 – 4471K → N in AAA28732. (PubMed:10731132)Curated
Sequence conflicti472 – 4743PQA → RRL in AAA28481. (PubMed:1881906)Curated
Sequence conflicti475 – 49824Missing in AAA28481. (PubMed:1881906)CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80559 mRNA. Translation: AAB21408.1.
M65016 mRNA. Translation: AAA28481.1.
M93149 mRNA. Translation: AAA28732.1.
AE014134 Genomic DNA. Translation: AAF53209.1.
BT010239 mRNA. Translation: AAQ23557.1. Frameshift.
M81958 Transcribed RNA. Translation: AAA28830.2.
PIRiA56564.
RefSeqiNP_523558.2. NM_078834.3. [P31369-1]
UniGeneiDm.4704.

Genome annotation databases

EnsemblMetazoaiFBtr0080393; FBpp0079974; FBgn0004394. [P31369-1]
GeneIDi34673.
KEGGidme:Dmel_CG12287.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S80559 mRNA. Translation: AAB21408.1 .
M65016 mRNA. Translation: AAA28481.1 .
M93149 mRNA. Translation: AAA28732.1 .
AE014134 Genomic DNA. Translation: AAF53209.1 .
BT010239 mRNA. Translation: AAQ23557.1 . Frameshift.
M81958 Transcribed RNA. Translation: AAA28830.2 .
PIRi A56564.
RefSeqi NP_523558.2. NM_078834.3. [P31369-1 ]
UniGenei Dm.4704.

3D structure databases

ProteinModelPortali P31369.
SMRi P31369. Positions 289-451.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 60717. 1 interaction.

Proteomic databases

PaxDbi P31369.
PRIDEi P31369.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai FBtr0080393 ; FBpp0079974 ; FBgn0004394 . [P31369-1 ]
GeneIDi 34673.
KEGGi dme:Dmel_CG12287.

Organism-specific databases

CTDi 34673.
FlyBasei FBgn0004394. pdm2.

Phylogenomic databases

eggNOGi NOG267922.
GeneTreei ENSGT00760000118935.
KOi K09364.
OrthoDBi EOG7DJSMG.

Enzyme and pathway databases

Reactomei REACT_208061. RNA Polymerase III Abortive And Retractive Initiation.
REACT_217491. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

GenomeRNAii 34673.
NextBioi 789620.

Gene expression databases

Bgeei P31369.
ExpressionAtlasi P31369. differential.

Family and domain databases

Gene3Di 1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProi IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
[Graphical view ]
Pfami PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view ]
PRINTSi PR00028. POUDOMAIN.
SMARTi SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view ]
SUPFAMi SSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEi PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of two Drosophila POU domain genes, related to oct-1 and oct-2, and the regulation of their expression patterns."
    Lloyd A., Sakonju S.
    Mech. Dev. 36:87-102(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
  2. "Two closely linked Drosophila POU domain genes are expressed in neuroblasts and sensory elements."
    Dick T., Yang X., Yeo S., Chia W.
    Proc. Natl. Acad. Sci. U.S.A. 88:7645-7649(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  3. "dOct2, a Drosophila Oct transcription factor that functions in yeast."
    Prakash K., Fang X.D., Engelberg D., Behal A., Parker C.S.
    Proc. Natl. Acad. Sci. U.S.A. 89:7080-7084(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY.
    Tissue: Embryo.
  4. "The genome sequence of Drosophila melanogaster."
    Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D., Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F., George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N., Sutton G.G., Wortman J.R., Yandell M.D.
    , Zhang Q., Chen L.X., Brandon R.C., Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C., Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A., An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A., Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V., Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J., Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E., Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B., Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I., Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C., Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S., Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M., Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M., Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D., Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F., Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D., Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A., Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C., McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C., Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L., Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R., Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V., Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F., Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J., Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R., Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y., Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T., Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S., Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W., Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M., Venter J.C.
    Science 287:2185-2195(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Berkeley.
  5. Cited for: GENOME REANNOTATION, ALTERNATIVE SPLICING.
    Strain: Berkeley.
  6. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: Berkeley.
    Tissue: Embryo.
  7. "Isolation of a family of Drosophila POU domain genes expressed in early development."
    Billin A.N., Cockerill K.A., Poole S.J.
    Mech. Dev. 34:75-84(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 68-498, FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
    Strain: Oregon-R.
    Tissue: Embryo.
  8. "Phosphoproteome analysis of Drosophila melanogaster embryos."
    Zhai B., Villen J., Beausoleil S.A., Mintseris J., Gygi S.P.
    J. Proteome Res. 7:1675-1682(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-72; SER-211; SER-215; SER-217 AND SER-219, IDENTIFICATION BY MASS SPECTROMETRY.
    Tissue: Embryo.

Entry informationi

Entry nameiPDM2A_DROME
AccessioniPrimary (citable) accession number: P31369
Secondary accession number(s): Q24430, Q6NR41, Q9VK70
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 127 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3