Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Protein nubbin

Gene

nub

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

DNA-binding regulatory protein implicated in early development. Involved in neuronal cell fate decision. Repressed directly or indirectly by the BX-C homeotic proteins.3 Publications

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi523 – 582HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

GO - Biological processi

  • dendrite morphogenesis Source: FlyBase
  • ganglion mother cell fate determination Source: FlyBase
  • heterochromatin organization involved in chromatin silencing Source: FlyBase
  • limb joint morphogenesis Source: FlyBase
  • negative regulation of antibacterial peptide biosynthetic process Source: FlyBase
  • pattern specification process Source: FlyBase
  • regulation of transcription from RNA polymerase II promoter Source: FlyBase
  • ventral cord development Source: FlyBase
  • wing disc development Source: FlyBase

Keywordsi

Molecular functionDevelopmental protein, DNA-binding
Biological processTranscription, Transcription regulation

Enzyme and pathway databases

ReactomeiR-DME-6807505. RNA polymerase II transcribes snRNA genes.
SignaLinkiP31368.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein nubbin
Alternative name(s):
POU domain protein 1
Short name:
PDM-1
Protein twain
dOCT1
dPOU-19
Gene namesi
Name:nub
Synonyms:OCT1, pdm-1, POU-19, TWN
ORF Names:CG34395
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraHolometabolaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0085424. nub.

Subcellular locationi

  • Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007771 – 601Protein nubbinAdd BLAST601

Proteomic databases

PaxDbiP31368.
PRIDEiP31368.

Expressioni

Tissue specificityi

Initial expression in cellular blastoderm stage, then in ectodermal stripes during germband extension. Broad expression in the neuroectoderm followed by limitation to discrete subsets of CNS cells, and expression in specific PNS neurons and support cells.2 Publications

Developmental stagei

Expressed both maternally and zygotically.3 Publications

Gene expression databases

BgeeiFBgn0085424.
ExpressionAtlasiP31368. differential.
GenevisibleiP31368. DM.

Interactioni

Protein-protein interaction databases

BioGridi60715. 27 interactors.
IntActiP31368. 4 interactors.
STRINGi7227.FBpp0111554.

Structurei

3D structure databases

ProteinModelPortaliP31368.
SMRiP31368.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini421 – 495POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi104 – 107Poly-His4
Compositional biasi191 – 201Poly-AlaAdd BLAST11
Compositional biasi359 – 364Poly-His6
Compositional biasi407 – 415Poly-Ala9

Sequence similaritiesi

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
InParanoidiP31368.
KOiK09364.
OrthoDBiEOG091G0U0S.

Family and domain databases

InterProiView protein in InterPro
IPR009057. Homeobox-like.
IPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
PfamiView protein in Pfam
PF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiView protein in SMART
SM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiView protein in PROSITE
PS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.

Sequencei

Sequence statusi: Complete.

P31368-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVMSELRWHT ASPEDNKNSL KRDLLKSTPT SAREAAVHIM QNRYISRLSR
60 70 80 90 100
SPSPLQSNAS DCDDNNSSVG TSSDRCRSPL SPALSLSHQQ AKRQLMSLQP
110 120 130 140 150
HPAHHHHNPH HLNHLNHHQY KQEEDYDDAN GGALNLTSDN SRHSTQSPSN
160 170 180 190 200
SVKSATASPV PVISVPSPVP PMISPVLAPS GCGSTTPNSM AAAAAAAAAV
210 220 230 240 250
ASTMGSGISP LLALPGMSSP QAQLAAAGLG MNNPLLTGSL SPQDFAQFHQ
260 270 280 290 300
LLQQRQVALT QQFNSYMELL RSGSLGLAQD DPALTAQVAA AQFLMQSQLQ
310 320 330 340 350
ALSQASQQLQ ALQKQQQRQV DEPLQLNHKM TQQPRSSTPH SIRSPIAIRS
360 370 380 390 400
PASSPQQLHH HHHHPLQITP PSSAASLKLS GMLTPSTPTS GTQMSQGTTT
410 420 430 440 450
PQPKTVASAA AARAAGEPSP EETTDLEELE QFAKTFKQRR IKLGFTQGDV
460 470 480 490 500
GLAMGKLYGN DFSQTTISRF EALNLSFKNM CKLKPLLQKW LDDADRTIQA
510 520 530 540 550
TGGVFDPAAL QATVSTPEII GRRRKKRTSI ETTIRGALEK AFLANQKPTS
560 570 580 590 600
EEITQLADRL SMEKEVVRVW FCNRRQKEKR INPSLDSPTG ADDDESSYMM

H
Length:601
Mass (Da):65,202
Last modified:July 1, 1993 - v1
Checksum:i8FFCD7B3C162D2B2
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti420P → R in AAB21409 (PubMed:1685891).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M81957 mRNA. Translation: AAA28829.1.
S80561 mRNA. Translation: AAB21409.1.
M65015 mRNA. Translation: AAA28480.1.
AE014134 Genomic DNA. Translation: AAF53205.2.
AY118387 mRNA. Translation: AAM48416.1.
PIRiB56564.
RefSeqiNP_001285876.1. NM_001298947.1.
NP_476659.1. NM_057311.5.
UniGeneiDm.4744.

Genome annotation databases

EnsemblMetazoaiFBtr0112643; FBpp0111555; FBgn0085424.
FBtr0342628; FBpp0309559; FBgn0085424.
GeneIDi34669.
KEGGidme:Dmel_CG34395.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiPDM1_DROME
AccessioniPrimary (citable) accession number: P31368
Secondary accession number(s): Q9VK74
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 10, 2017
This is version 150 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families