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P31364 (P3F4A_XENLA) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 92. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 3, transcription factor 4-A
Alternative name(s):
Transcription factor POU2
Short name=XlPOU 2
Short name=XlPOU2
Gene names
Name:pou3f4-a
Synonyms:pou2, pou3f4
OrganismXenopus laevis (African clawed frog)
Taxonomic identifier8355 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

Protein attributes

Sequence length361 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcriptional activator. Induces neural-specific gene expression to act as a key regulator of neural differentiation. Ref.1 Ref.4

Subcellular location

Nucleus.

Tissue specificity

From embryonic stage 10, expressed in the Spemann's organizer. During gastrulation, expressed in both the involuting mesoderm and the overlying neuroectoderm. During the neural plate and neural fold stages, expressed in the entire neuroectoderm with expression in discrete regions of the developing nervous system persisting at later stages. Transiently expressed in the pronephros from stages 24-32. In adults, expressed in the kidney and brain. Ref.1 Ref.3

Developmental stage

First expressed at embryonic stage 9, with expression increasing at neurula stage and again at the tailbud stage. Expression persists through development to adulthood. Ref.1 Ref.3

Induction

By nog, zic1 and zic3. Ref.1 Ref.4

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Sequence caution

The sequence CAA41781.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 361361POU domain, class 3, transcription factor 4-A
PRO_0000100765

Regions

Domain186 – 26075POU-specific
DNA binding278 – 33760Homeobox

Experimental info

Sequence conflict1271S → G in CAA41781. Ref.3

Sequences

Sequence LengthMass (Da)Tools
P31364 [UniParc].

Last modified December 15, 2009. Version 2.
Checksum: 6F6BE1D6AC844F81

FASTA36139,838
        10         20         30         40         50         60 
MATAASNPYS ILSSSSLVHA DSAVMQQGSP FRNPQKLLQS DYLQGVPCNG HPLGHHWVTS 

        70         80         90        100        110        120 
LSDANPWSSS LASSPLDQQD IKPGREDLQL GAIIHHRSPH VNHHSPHTNH PNAWGASPAH 

       130        140        150        160        170        180 
NSSLTSSGQP INIYSQPSFT VSGMLDHGEL TPPLPAGTTQ SLHPVLREPN DHVDLGSHHC 

       190        200        210        220        230        240 
QDHSDEETPT SDELEQFAKQ FKQRRIKLGF TQADVGLALG TLYGNVFSQT TICRFEALQL 

       250        260        270        280        290        300 
SFKNMCKLKP LLNKWLEEAD SSTGNPTSID KIAAQGRKRK KRTSIEVSVK GVLETHFLKC 

       310        320        330        340        350        360 
PKPAALEITS LADSLQLEKE VVRVWFCNRR QKEKRMTPPG DPQQHEVYSH SVKTDTSCNE 


L 

« Hide

References

« Hide 'large scale' references
[1]"XLPOU 2, a noggin-inducible gene, has direct neuralizing activity."
Witta S.E., Agarwal V.R., Sato S.M.
Development 121:721-730(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
Tissue: Brain.
[2]NIH - Xenopus Gene Collection (XGC) project
Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Kidney.
[3]"XLPOU 1 and XLPOU 2, two novel POU domain genes expressed in the dorsoanterior region of Xenopus embryos."
Agarwal V.R., Sato S.M.
Dev. Biol. 147:363-373(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 127-361, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
Tissue: Brain.
[4]"The POU domain gene, XlPOU 2 is an essential downstream determinant of neural induction."
Matsuo-Takasaki M., Lim J.H., Sato S.M.
Mech. Dev. 89:75-85(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, INDUCTION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U17654 mRNA. Translation: AAA92684.1.
BC076747 mRNA. Translation: AAH76747.1.
X59055 mRNA. Translation: CAA41781.1. Different initiation.
RefSeqNP_001094393.1. NM_001100923.1.
UniGeneXl.1023.

3D structure databases

ProteinModelPortalP31364.
SMRP31364. Positions 192-338.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID397929.
KEGGxla:397929.

Organism-specific databases

CTD5456.
XenbaseXB-GENE-865294. pou3f4.

Phylogenomic databases

HOVERGENHBG053120.
KOK09365.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameP3F4A_XENLA
AccessionPrimary (citable) accession number: P31364
Secondary accession number(s): Q6DFJ0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 15, 2009
Last modified: February 19, 2014
This is version 92 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families