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P31364

- P3F4A_XENLA

UniProt

P31364 - P3F4A_XENLA

Protein

POU domain, class 3, transcription factor 4-A

Gene

pou3f4-a

Organism
Xenopus laevis (African clawed frog)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 93 (01 Oct 2014)
      Sequence version 2 (15 Dec 2009)
      Previous versions | rss
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    Functioni

    Transcriptional activator. Induces neural-specific gene expression to act as a key regulator of neural differentiation.2 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi278 – 33760HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. sequence-specific DNA binding Source: InterPro
    2. sequence-specific DNA binding transcription factor activity Source: InterPro

    GO - Biological processi

    1. multicellular organismal development Source: UniProtKB-KW
    2. transcription, DNA-templated Source: UniProtKB-KW

    Keywords - Molecular functioni

    Activator, Developmental protein

    Keywords - Biological processi

    Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 3, transcription factor 4-A
    Alternative name(s):
    Transcription factor POU2
    Short name:
    XlPOU 2
    Short name:
    XlPOU2
    Gene namesi
    Name:pou3f4-a
    Synonyms:pou2, pou3f4
    OrganismiXenopus laevis (African clawed frog)
    Taxonomic identifieri8355 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiAmphibiaBatrachiaAnuraPipoideaPipidaeXenopodinaeXenopusXenopus

    Organism-specific databases

    XenbaseiXB-GENE-865294. pou3f4.

    Subcellular locationi

    GO - Cellular componenti

    1. nucleus Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 361361POU domain, class 3, transcription factor 4-APRO_0000100765Add
    BLAST

    Expressioni

    Tissue specificityi

    From embryonic stage 10, expressed in the Spemann's organizer. During gastrulation, expressed in both the involuting mesoderm and the overlying neuroectoderm. During the neural plate and neural fold stages, expressed in the entire neuroectoderm with expression in discrete regions of the developing nervous system persisting at later stages. Transiently expressed in the pronephros from stages 24-32. In adults, expressed in the kidney and brain.2 Publications

    Developmental stagei

    First expressed at embryonic stage 9, with expression increasing at neurula stage and again at the tailbud stage. Expression persists through development to adulthood.2 Publications

    Inductioni

    By nog, zic1 and zic3.2 Publications

    Structurei

    3D structure databases

    ProteinModelPortaliP31364.
    SMRiP31364. Positions 192-338.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini186 – 26075POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    HOVERGENiHBG053120.
    KOiK09365.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31364-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATAASNPYS ILSSSSLVHA DSAVMQQGSP FRNPQKLLQS DYLQGVPCNG    50
    HPLGHHWVTS LSDANPWSSS LASSPLDQQD IKPGREDLQL GAIIHHRSPH 100
    VNHHSPHTNH PNAWGASPAH NSSLTSSGQP INIYSQPSFT VSGMLDHGEL 150
    TPPLPAGTTQ SLHPVLREPN DHVDLGSHHC QDHSDEETPT SDELEQFAKQ 200
    FKQRRIKLGF TQADVGLALG TLYGNVFSQT TICRFEALQL SFKNMCKLKP 250
    LLNKWLEEAD SSTGNPTSID KIAAQGRKRK KRTSIEVSVK GVLETHFLKC 300
    PKPAALEITS LADSLQLEKE VVRVWFCNRR QKEKRMTPPG DPQQHEVYSH 350
    SVKTDTSCNE L 361
    Length:361
    Mass (Da):39,838
    Last modified:December 15, 2009 - v2
    Checksum:i6F6BE1D6AC844F81
    GO

    Sequence cautioni

    The sequence CAA41781.1 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti127 – 1271S → G in CAA41781. (PubMed:1717323)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17654 mRNA. Translation: AAA92684.1.
    BC076747 mRNA. Translation: AAH76747.1.
    X59055 mRNA. Translation: CAA41781.1. Different initiation.
    RefSeqiNP_001094393.1. NM_001100923.1.
    UniGeneiXl.1023.

    Genome annotation databases

    GeneIDi397929.
    KEGGixla:397929.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U17654 mRNA. Translation: AAA92684.1 .
    BC076747 mRNA. Translation: AAH76747.1 .
    X59055 mRNA. Translation: CAA41781.1 . Different initiation.
    RefSeqi NP_001094393.1. NM_001100923.1.
    UniGenei Xl.1023.

    3D structure databases

    ProteinModelPortali P31364.
    SMRi P31364. Positions 192-338.
    ModBasei Search...
    MobiDBi Search...

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 397929.
    KEGGi xla:397929.

    Organism-specific databases

    CTDi 5456.
    Xenbasei XB-GENE-865294. pou3f4.

    Phylogenomic databases

    HOVERGENi HBG053120.
    KOi K09365.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "XLPOU 2, a noggin-inducible gene, has direct neuralizing activity."
      Witta S.E., Agarwal V.R., Sato S.M.
      Development 121:721-730(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE, INDUCTION.
      Tissue: Brain.
    2. NIH - Xenopus Gene Collection (XGC) project
      Submitted (JUL-2004) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Kidney.
    3. "XLPOU 1 and XLPOU 2, two novel POU domain genes expressed in the dorsoanterior region of Xenopus embryos."
      Agarwal V.R., Sato S.M.
      Dev. Biol. 147:363-373(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 127-361, TISSUE SPECIFICITY, DEVELOPMENTAL STAGE.
      Tissue: Brain.
    4. "The POU domain gene, XlPOU 2 is an essential downstream determinant of neural induction."
      Matsuo-Takasaki M., Lim J.H., Sato S.M.
      Mech. Dev. 89:75-85(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, INDUCTION.

    Entry informationi

    Entry nameiP3F4A_XENLA
    AccessioniPrimary (citable) accession number: P31364
    Secondary accession number(s): Q6DFJ0
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: December 15, 2009
    Last modified: October 1, 2014
    This is version 93 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3