ID   PO3F3_MOUSE             Reviewed;         497 AA.
AC   P31361;
DT   01-JUL-1993, integrated into UniProtKB/Swiss-Prot.
DT   27-JUL-2011, sequence version 2.
DT   27-MAR-2024, entry version 177.
DE   RecName: Full=POU domain, class 3, transcription factor 3;
DE   AltName: Full=Brain-specific homeobox/POU domain protein 1;
DE            Short=Brain-1;
DE            Short=Brn-1;
DE   AltName: Full=Octamer-binding protein 8;
DE            Short=Oct-8;
DE   AltName: Full=Octamer-binding transcription factor 8;
DE            Short=OTF-8;
GN   Name=Pou3f3; Synonyms=Brn-1, Brn1, Otf8;
OS   Mus musculus (Mouse).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Mus; Mus.
OX   NCBI_TaxID=10090;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1565620; DOI=10.1073/pnas.89.8.3280;
RA   Hara Y., Rovescalli C., Kim Y., Nirenberg M.;
RT   "Structure and evolution of four POU domain genes expressed in mouse
RT   brain.";
RL   Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=C57BL/6J;
RX   PubMed=19468303; DOI=10.1371/journal.pbio.1000112;
RA   Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X.,
RA   Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y.,
RA   Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S.,
RA   Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R.,
RA   Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K.,
RA   Eichler E.E., Ponting C.P.;
RT   "Lineage-specific biology revealed by a finished genome assembly of the
RT   mouse.";
RL   PLoS Biol. 7:E1000112-E1000112(2009).
RN   [3]
RP   FUNCTION, AND SUBCELLULAR LOCATION.
RX   PubMed=10473120; DOI=10.1016/s0925-4773(99)00067-2;
RA   Mihailescu D., Kuery P., Monard D.;
RT   "An octamer-binding site is crucial for the activity of an enhancer active
RT   at the embryonic met-/mesencephalic junction.";
RL   Mech. Dev. 84:55-67(1999).
RN   [4]
RP   FUNCTION, DEVELOPMENTAL STAGE, AND DISRUPTION PHENOTYPE.
RX   PubMed=12130536; DOI=10.1101/gad.978002;
RA   Sugitani Y., Nakai S., Minowa O., Nishi M., Jishage K., Kawano H., Mori K.,
RA   Ogawa M., Noda T.;
RT   "Brn-1 and Brn-2 share crucial roles in the production and positioning of
RT   mouse neocortical neurons.";
RL   Genes Dev. 16:1760-1765(2002).
RN   [5]
RP   DISRUPTION PHENOTYPE.
RX   PubMed=11859196; DOI=10.1126/science.1067132;
RA   McEvilly R.J., de Diaz M.O., Schonemann M.D., Hooshmand F., Rosenfeld M.G.;
RT   "Transcriptional regulation of cortical neuron migration by POU domain
RT   factors.";
RL   Science 295:1528-1532(2002).
RN   [6]
RP   FUNCTION.
RX   PubMed=19527706; DOI=10.1016/j.ydbio.2009.06.011;
RA   Wolf M., Lommes P., Sock E., Reiprich S., Friedrich R.P., Kriesch J.,
RA   Stolt C.C., Bermingham J.R. Jr., Wegner M.;
RT   "Replacement of related POU transcription factors leads to severe defects
RT   in mouse forebrain development.";
RL   Dev. Biol. 332:418-428(2009).
CC   -!- FUNCTION: Transcription factor that acts synergistically with SOX11 and
CC       SOX4 (By similarity). Plays a role in neuronal development. Is
CC       implicated in an enhancer activity at the embryonic met-mesencephalic
CC       junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-
CC       3'). {ECO:0000250|UniProtKB:Q63262, ECO:0000269|PubMed:10473120,
CC       ECO:0000269|PubMed:12130536, ECO:0000269|PubMed:19527706}.
CC   -!- SUBUNIT: Homodimer. {ECO:0000250|UniProtKB:P20264}.
CC   -!- SUBCELLULAR LOCATION: Nucleus {ECO:0000255|PROSITE-ProRule:PRU00108,
CC       ECO:0000255|PROSITE-ProRule:PRU00530, ECO:0000269|PubMed:10473120}.
CC   -!- TISSUE SPECIFICITY: Brain.
CC   -!- DEVELOPMENTAL STAGE: Expressed in the developing neocortex, both in the
CC       late precursor cells and in the migrating neurons.
CC       {ECO:0000269|PubMed:12130536}.
CC   -!- DISRUPTION PHENOTYPE: Neonatal death at least 36 hours postpartum. Loss
CC       of both Pou3f3 and Pou3f2 leads to abnormal formation of the neocortex
CC       with dramatically reduced production of layer IV-II neurons and
CC       defective migration of neurons. {ECO:0000269|PubMed:11859196,
CC       ECO:0000269|PubMed:12130536}.
CC   -!- MISCELLANEOUS: Can fully replace Pou3f1 in Schwann cells of embryonic
CC       mice and rescue peripheral nervous system development in these animals,
CC       although severe defects in forebrain development are still observed.
CC   -!- SIMILARITY: Belongs to the POU transcription factor family. Class-3
CC       subfamily. {ECO:0000305}.
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DR   EMBL; M88299; AAA39960.1; -; Genomic_DNA.
DR   EMBL; AC122898; -; NOT_ANNOTATED_CDS; Genomic_DNA.
DR   CCDS; CCDS14917.1; -.
DR   PIR; S31223; S31223.
DR   RefSeq; NP_032926.2; NM_008900.2.
DR   AlphaFoldDB; P31361; -.
DR   SMR; P31361; -.
DR   BioGRID; 202307; 6.
DR   STRING; 10090.ENSMUSP00000052088; -.
DR   iPTMnet; P31361; -.
DR   PhosphoSitePlus; P31361; -.
DR   MaxQB; P31361; -.
DR   PaxDb; 10090-ENSMUSP00000052088; -.
DR   PeptideAtlas; P31361; -.
DR   ProteomicsDB; 289355; -.
DR   Antibodypedia; 32979; 178 antibodies from 33 providers.
DR   DNASU; 18993; -.
DR   Ensembl; ENSMUST00000054883.4; ENSMUSP00000052088.3; ENSMUSG00000045515.5.
DR   Ensembl; ENSMUST00000239323.2; ENSMUSP00000159226.2; ENSMUSG00000045515.5.
DR   GeneID; 18993; -.
DR   KEGG; mmu:18993; -.
DR   UCSC; uc007auw.1; mouse.
DR   AGR; MGI:102564; -.
DR   MGI; MGI:102564; Pou3f3.
DR   VEuPathDB; HostDB:ENSMUSG00000045515; -.
DR   eggNOG; KOG3802; Eukaryota.
DR   GeneTree; ENSGT00940000163665; -.
DR   HOGENOM; CLU_013065_1_2_1; -.
DR   InParanoid; P31361; -.
DR   OMA; PPHQGHW; -.
DR   OrthoDB; 4250502at2759; -.
DR   PhylomeDB; P31361; -.
DR   TreeFam; TF316413; -.
DR   BioGRID-ORCS; 18993; 3 hits in 77 CRISPR screens.
DR   ChiTaRS; Pou3f3; mouse.
DR   PRO; PR:P31361; -.
DR   Proteomes; UP000000589; Chromosome 1.
DR   RNAct; P31361; Protein.
DR   Bgee; ENSMUSG00000045515; Expressed in medial ganglionic eminence and 198 other cell types or tissues.
DR   GO; GO:0005654; C:nucleoplasm; ISO:MGI.
DR   GO; GO:0005634; C:nucleus; IDA:MGI.
DR   GO; GO:0003677; F:DNA binding; IDA:MGI.
DR   GO; GO:0003700; F:DNA-binding transcription factor activity; ISO:MGI.
DR   GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; ISO:MGI.
DR   GO; GO:0071837; F:HMG box domain binding; IPI:UniProtKB.
DR   GO; GO:0042803; F:protein homodimerization activity; ISS:UniProtKB.
DR   GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IBA:GO_Central.
DR   GO; GO:0043565; F:sequence-specific DNA binding; IDA:UniProtKB.
DR   GO; GO:0021799; P:cerebral cortex radially oriented cell migration; IGI:MGI.
DR   GO; GO:0048878; P:chemical homeostasis; IMP:MGI.
DR   GO; GO:0021869; P:forebrain ventricular zone progenitor cell division; IGI:MGI.
DR   GO; GO:0001822; P:kidney development; IMP:MGI.
DR   GO; GO:0072218; P:metanephric ascending thin limb development; IMP:UniProtKB.
DR   GO; GO:0072240; P:metanephric DCT cell differentiation; IMP:UniProtKB.
DR   GO; GO:0072236; P:metanephric loop of Henle development; IMP:UniProtKB.
DR   GO; GO:0072227; P:metanephric macula densa development; IMP:UniProtKB.
DR   GO; GO:0072233; P:metanephric thick ascending limb development; IMP:UniProtKB.
DR   GO; GO:0043066; P:negative regulation of apoptotic process; IMP:UniProtKB.
DR   GO; GO:0045892; P:negative regulation of DNA-templated transcription; IDA:UniProtKB.
DR   GO; GO:0000122; P:negative regulation of transcription by RNA polymerase II; ISO:MGI.
DR   GO; GO:0008284; P:positive regulation of cell population proliferation; IMP:UniProtKB.
DR   GO; GO:0045893; P:positive regulation of DNA-templated transcription; IMP:UniProtKB.
DR   GO; GO:0010628; P:positive regulation of gene expression; ISO:MGI.
DR   GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IGI:MGI.
DR   GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IBA:GO_Central.
DR   GO; GO:0071918; P:urea transmembrane transport; IMP:MGI.
DR   CDD; cd00086; homeodomain; 1.
DR   Gene3D; 1.10.10.60; Homeodomain-like; 1.
DR   Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR017970; Homeobox_CS.
DR   InterPro; IPR001356; Homeobox_dom.
DR   InterPro; IPR010982; Lambda_DNA-bd_dom_sf.
DR   InterPro; IPR013847; POU.
DR   InterPro; IPR000327; POU_dom.
DR   InterPro; IPR016362; TF_POU_3.
DR   PANTHER; PTHR11636; POU DOMAIN; 1.
DR   PANTHER; PTHR11636:SF125; POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 3; 1.
DR   Pfam; PF00046; Homeodomain; 1.
DR   Pfam; PF00157; Pou; 1.
DR   PIRSF; PIRSF002629; Transcription_factor_POU; 1.
DR   PRINTS; PR00028; POUDOMAIN.
DR   SMART; SM00389; HOX; 1.
DR   SMART; SM00352; POU; 1.
DR   SUPFAM; SSF46689; Homeodomain-like; 1.
DR   SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1.
DR   PROSITE; PS00027; HOMEOBOX_1; 1.
DR   PROSITE; PS50071; HOMEOBOX_2; 1.
DR   PROSITE; PS00035; POU_1; 1.
DR   PROSITE; PS00465; POU_2; 1.
DR   PROSITE; PS51179; POU_3; 1.
DR   Genevisible; P31361; MM.
PE   2: Evidence at transcript level;
KW   Developmental protein; DNA-binding; Homeobox; Neurogenesis; Nucleus;
KW   Reference proteome; Transcription; Transcription regulation.
FT   CHAIN           1..497
FT                   /note="POU domain, class 3, transcription factor 3"
FT                   /id="PRO_0000100728"
FT   DOMAIN          311..385
FT                   /note="POU-specific"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00530"
FT   DNA_BIND        403..462
FT                   /note="Homeobox"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00108"
FT   REGION          32..62
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          121..189
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          230..316
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          458..497
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        132..147
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        168..182
FT                   /note="Pro residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        270..287
FT                   /note="Basic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        462..497
FT                   /note="Polar residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   CONFLICT        10
FT                   /note="L -> Q (in Ref. 1; AAA39960)"
FT                   /evidence="ECO:0000305"
FT   CONFLICT        81..82
FT                   /note="Missing (in Ref. 1; AAA39960)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   497 AA;  50200 MW;  4B1860BEED53F8B0 CRC64;
     MATAASNPYL PGNSLLTAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM QPGSAAVTSG
     AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL PHAAAAAAAA AAAAVEASSP
     WSGSAVGMAG SPQQPPQPPP PPPQGPDVKG GAGREDLHAG TALHHRGPPH LGPPPPPPHQ
     GHPGGWGAAA AAAAAAAAAA AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG
     GGGGGAGGGA QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP
     GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN VFSQTTICRF
     EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ GRKRKKRTSI EVSVKGALES
     HFLKCPKPSA QEITNLADSL QLEKEVVRVW FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT
     VSADTPPPHH GLQTSVQ
//