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Protein

POU domain, class 3, transcription factor 3

Gene

Pou3f3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that acts synergistically with SOX11 and SOX4 (By similarity). Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3').By similarity3 Publications

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi403 – 46260HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

  1. DNA binding Source: MGI
  2. HMG box domain binding Source: UniProtKB
  3. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
  4. sequence-specific DNA binding Source: UniProtKB

GO - Biological processi

  1. cerebral cortex radially oriented cell migration Source: MGI
  2. forebrain ventricular zone progenitor cell division Source: MGI
  3. kidney development Source: MGI
  4. metanephric ascending thin limb development Source: UniProtKB
  5. metanephric DCT cell differentiation Source: UniProtKB
  6. metanephric loop of Henle development Source: UniProtKB
  7. metanephric macula densa development Source: UniProtKB
  8. metanephric thick ascending limb development Source: UniProtKB
  9. negative regulation of apoptotic process Source: UniProtKB
  10. negative regulation of transcription, DNA-templated Source: UniProtKB
  11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
  12. positive regulation of cell proliferation Source: UniProtKB
  13. positive regulation of transcription, DNA-templated Source: UniProtKB
  14. positive regulation of transcription from RNA polymerase II promoter Source: MGI
  15. transcription from RNA polymerase II promoter Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Neurogenesis, Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 3
Alternative name(s):
Brain-specific homeobox/POU domain protein 1
Short name:
Brain-1
Short name:
Brn-1
Octamer-binding protein 8
Short name:
Oct-8
Octamer-binding transcription factor 8
Short name:
OTF-8
Gene namesi
Name:Pou3f3
Synonyms:Brn-1, Brn1, Otf8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 1

Organism-specific databases

MGIiMGI:102564. Pou3f3.

Subcellular locationi

Nucleus PROSITE-ProRule annotation1 Publication

GO - Cellular componenti

  1. nucleus Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

Pathology & Biotechi

Disruption phenotypei

Neonatal death at least 36 hours postpartum. Loss of both Pou3f3 and Pou3f2 leads to abnormal formation of the neocortex with dramatically reduced production of layer IV-II neurons and defective migration of neurons.2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 497497POU domain, class 3, transcription factor 3PRO_0000100728Add
BLAST

Proteomic databases

MaxQBiP31361.
PRIDEiP31361.

PTM databases

PhosphoSiteiP31361.

Expressioni

Tissue specificityi

Brain.

Developmental stagei

Expressed in the developing neocortex, both in the late precursor cells and in the migrating neurons.1 Publication

Gene expression databases

BgeeiP31361.
CleanExiMM_POU3F3.
GenevestigatoriP31361.

Interactioni

Protein-protein interaction databases

BioGridi202307. 3 interactions.
STRINGi10090.ENSMUSP00000052088.

Structurei

3D structure databases

ProteinModelPortaliP31361.
SMRiP31361. Positions 314-463.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini311 – 38575POU-specificPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi28 – 301274Gly-richAdd
BLAST
Compositional biasi80 – 203124Ala-richAdd
BLAST
Compositional biasi132 – 18352Pro-richAdd
BLAST
Compositional biasi269 – 30840His-richAdd
BLAST

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP31361.
KOiK09365.
OMAiAHLPSMA.
OrthoDBiEOG7DJSMG.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31361-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAASNPYL PGNSLLTAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM
60 70 80 90 100
QPGSAAVTSG AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL
110 120 130 140 150
PHAAAAAAAA AAAAVEASSP WSGSAVGMAG SPQQPPQPPP PPPQGPDVKG
160 170 180 190 200
GAGREDLHAG TALHHRGPPH LGPPPPPPHQ GHPGGWGAAA AAAAAAAAAA
210 220 230 240 250
AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG GGGGGAGGGA
260 270 280 290 300
QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP
310 320 330 340 350
GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN
360 370 380 390 400
VFSQTTICRF EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ
410 420 430 440 450
GRKRKKRTSI EVSVKGALES HFLKCPKPSA QEITNLADSL QLEKEVVRVW
460 470 480 490
FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT VSADTPPPHH GLQTSVQ
Length:497
Mass (Da):50,200
Last modified:July 27, 2011 - v2
Checksum:i4B1860BEED53F8B0
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti10 – 101L → Q in AAA39960 (PubMed:1565620).Curated
Sequence conflicti81 – 822Missing in AAA39960 (PubMed:1565620).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88299 Genomic DNA. Translation: AAA39960.1.
AC122898 Genomic DNA. No translation available.
CCDSiCCDS14917.1.
PIRiS31223.
RefSeqiNP_032926.2. NM_008900.2.
UniGeneiMm.489878.

Genome annotation databases

EnsembliENSMUST00000054883; ENSMUSP00000052088; ENSMUSG00000045515.
GeneIDi18993.
KEGGimmu:18993.
UCSCiuc007auw.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88299 Genomic DNA. Translation: AAA39960.1.
AC122898 Genomic DNA. No translation available.
CCDSiCCDS14917.1.
PIRiS31223.
RefSeqiNP_032926.2. NM_008900.2.
UniGeneiMm.489878.

3D structure databases

ProteinModelPortaliP31361.
SMRiP31361. Positions 314-463.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202307. 3 interactions.
STRINGi10090.ENSMUSP00000052088.

PTM databases

PhosphoSiteiP31361.

Proteomic databases

MaxQBiP31361.
PRIDEiP31361.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000054883; ENSMUSP00000052088; ENSMUSG00000045515.
GeneIDi18993.
KEGGimmu:18993.
UCSCiuc007auw.1. mouse.

Organism-specific databases

CTDi5455.
MGIiMGI:102564. Pou3f3.

Phylogenomic databases

eggNOGiNOG261729.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP31361.
KOiK09365.
OMAiAHLPSMA.
OrthoDBiEOG7DJSMG.
TreeFamiTF316413.

Miscellaneous databases

NextBioi295390.
PROiP31361.
SOURCEiSearch...

Gene expression databases

BgeeiP31361.
CleanExiMM_POU3F3.
GenevestigatoriP31361.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure and evolution of four POU domain genes expressed in mouse brain."
    Hara Y., Rovescalli C., Kim Y., Nirenberg M.
    Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: C57BL/6J.
  3. "An octamer-binding site is crucial for the activity of an enhancer active at the embryonic met-/mesencephalic junction."
    Mihailescu D., Kuery P., Monard D.
    Mech. Dev. 84:55-67(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, SUBCELLULAR LOCATION.
  4. "Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons."
    Sugitani Y., Nakai S., Minowa O., Nishi M., Jishage K., Kawano H., Mori K., Ogawa M., Noda T.
    Genes Dev. 16:1760-1765(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
  5. "Transcriptional regulation of cortical neuron migration by POU domain factors."
    McEvilly R.J., de Diaz M.O., Schonemann M.D., Hooshmand F., Rosenfeld M.G.
    Science 295:1528-1532(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  6. "Replacement of related POU transcription factors leads to severe defects in mouse forebrain development."
    Wolf M., Lommes P., Sock E., Reiprich S., Friedrich R.P., Kriesch J., Stolt C.C., Bermingham J.R. Jr., Wegner M.
    Dev. Biol. 332:418-428(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPO3F3_MOUSE
AccessioniPrimary (citable) accession number: P31361
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 27, 2011
Last modified: January 7, 2015
This is version 126 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

Can fully replace Pou3f1 in Schwann cells of embryonic mice and rescue peripheral nervous system development in these animals, although severe defects in forebrain development are still observed.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.