Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P31361

- PO3F3_MOUSE

UniProt

P31361 - PO3F3_MOUSE

Protein

POU domain, class 3, transcription factor 3

Gene

Pou3f3

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 124 (01 Oct 2014)
      Sequence version 2 (27 Jul 2011)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Transcription factor that acts synergistically with SOX11 and SOX4 By similarity. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3').By similarity3 Publications

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    DNA bindingi403 – 46260HomeoboxPROSITE-ProRule annotationAdd
    BLAST

    GO - Molecular functioni

    1. DNA binding Source: MGI
    2. HMG box domain binding Source: UniProtKB
    3. protein binding Source: UniProtKB
    4. RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity Source: MGI
    5. sequence-specific DNA binding Source: UniProtKB

    GO - Biological processi

    1. cerebral cortex radially oriented cell migration Source: MGI
    2. forebrain ventricular zone progenitor cell division Source: MGI
    3. kidney development Source: MGI
    4. metanephric ascending thin limb development Source: UniProtKB
    5. metanephric DCT cell differentiation Source: UniProtKB
    6. metanephric loop of Henle development Source: UniProtKB
    7. metanephric macula densa development Source: UniProtKB
    8. metanephric thick ascending limb development Source: UniProtKB
    9. negative regulation of apoptotic process Source: UniProtKB
    10. negative regulation of transcription, DNA-templated Source: UniProtKB
    11. negative regulation of transcription from RNA polymerase II promoter Source: MGI
    12. positive regulation of cell proliferation Source: UniProtKB
    13. positive regulation of transcription, DNA-templated Source: UniProtKB
    14. positive regulation of transcription from RNA polymerase II promoter Source: MGI
    15. transcription from RNA polymerase II promoter Source: GOC

    Keywords - Molecular functioni

    Developmental protein

    Keywords - Biological processi

    Neurogenesis, Transcription, Transcription regulation

    Keywords - Ligandi

    DNA-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    POU domain, class 3, transcription factor 3
    Alternative name(s):
    Brain-specific homeobox/POU domain protein 1
    Short name:
    Brain-1
    Short name:
    Brn-1
    Octamer-binding protein 8
    Short name:
    Oct-8
    Octamer-binding transcription factor 8
    Short name:
    OTF-8
    Gene namesi
    Name:Pou3f3
    Synonyms:Brn-1, Brn1, Otf8
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 1

    Organism-specific databases

    MGIiMGI:102564. Pou3f3.

    Subcellular locationi

    Nucleus 1 PublicationPROSITE-ProRule annotation

    GO - Cellular componenti

    1. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    Nucleus

    Pathology & Biotechi

    Disruption phenotypei

    Neonatal death at least 36 hours postpartum. Loss of both Pou3f3 and Pou3f2 leads to abnormal formation of the neocortex with dramatically reduced production of layer IV-II neurons and defective migration of neurons.2 Publications

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 497497POU domain, class 3, transcription factor 3PRO_0000100728Add
    BLAST

    Proteomic databases

    MaxQBiP31361.
    PRIDEiP31361.

    PTM databases

    PhosphoSiteiP31361.

    Expressioni

    Tissue specificityi

    Brain.

    Developmental stagei

    Expressed in the developing neocortex, both in the late precursor cells and in the migrating neurons.1 Publication

    Gene expression databases

    BgeeiP31361.
    CleanExiMM_POU3F3.
    GenevestigatoriP31361.

    Interactioni

    Protein-protein interaction databases

    BioGridi202307. 3 interactions.
    STRINGi10090.ENSMUSP00000052088.

    Structurei

    3D structure databases

    ProteinModelPortaliP31361.
    SMRiP31361. Positions 314-463.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini311 – 38575POU-specificPROSITE-ProRule annotationAdd
    BLAST

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi28 – 301274Gly-richAdd
    BLAST
    Compositional biasi80 – 203124Ala-richAdd
    BLAST
    Compositional biasi132 – 18352Pro-richAdd
    BLAST
    Compositional biasi269 – 30840His-richAdd
    BLAST

    Sequence similaritiesi

    Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
    Contains 1 POU-specific domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Homeobox

    Phylogenomic databases

    eggNOGiNOG261729.
    GeneTreeiENSGT00730000110809.
    HOGENOMiHOG000116303.
    HOVERGENiHBG053120.
    InParanoidiP31361.
    KOiK09365.
    OMAiAHLPSMA.
    OrthoDBiEOG7DJSMG.
    TreeFamiTF316413.

    Family and domain databases

    Gene3Di1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProiIPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view]
    PfamiPF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view]
    PIRSFiPIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSiPR00028. POUDOMAIN.
    SMARTiSM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view]
    SUPFAMiSSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEiPS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31361-1 [UniParc]FASTAAdd to Basket

    « Hide

    MATAASNPYL PGNSLLTAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM    50
    QPGSAAVTSG AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL 100
    PHAAAAAAAA AAAAVEASSP WSGSAVGMAG SPQQPPQPPP PPPQGPDVKG 150
    GAGREDLHAG TALHHRGPPH LGPPPPPPHQ GHPGGWGAAA AAAAAAAAAA 200
    AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG GGGGGAGGGA 250
    QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP 300
    GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN 350
    VFSQTTICRF EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ 400
    GRKRKKRTSI EVSVKGALES HFLKCPKPSA QEITNLADSL QLEKEVVRVW 450
    FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT VSADTPPPHH GLQTSVQ 497
    Length:497
    Mass (Da):50,200
    Last modified:July 27, 2011 - v2
    Checksum:i4B1860BEED53F8B0
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti10 – 101L → Q in AAA39960. (PubMed:1565620)Curated
    Sequence conflicti81 – 822Missing in AAA39960. (PubMed:1565620)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M88299 Genomic DNA. Translation: AAA39960.1.
    AC122898 Genomic DNA. No translation available.
    CCDSiCCDS14917.1.
    PIRiS31223.
    RefSeqiNP_032926.2. NM_008900.2.
    UniGeneiMm.489878.

    Genome annotation databases

    EnsembliENSMUST00000054883; ENSMUSP00000052088; ENSMUSG00000045515.
    GeneIDi18993.
    KEGGimmu:18993.
    UCSCiuc007auw.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M88299 Genomic DNA. Translation: AAA39960.1 .
    AC122898 Genomic DNA. No translation available.
    CCDSi CCDS14917.1.
    PIRi S31223.
    RefSeqi NP_032926.2. NM_008900.2.
    UniGenei Mm.489878.

    3D structure databases

    ProteinModelPortali P31361.
    SMRi P31361. Positions 314-463.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 202307. 3 interactions.
    STRINGi 10090.ENSMUSP00000052088.

    PTM databases

    PhosphoSitei P31361.

    Proteomic databases

    MaxQBi P31361.
    PRIDEi P31361.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000054883 ; ENSMUSP00000052088 ; ENSMUSG00000045515 .
    GeneIDi 18993.
    KEGGi mmu:18993.
    UCSCi uc007auw.1. mouse.

    Organism-specific databases

    CTDi 5455.
    MGIi MGI:102564. Pou3f3.

    Phylogenomic databases

    eggNOGi NOG261729.
    GeneTreei ENSGT00730000110809.
    HOGENOMi HOG000116303.
    HOVERGENi HBG053120.
    InParanoidi P31361.
    KOi K09365.
    OMAi AHLPSMA.
    OrthoDBi EOG7DJSMG.
    TreeFami TF316413.

    Miscellaneous databases

    NextBioi 295390.
    PROi P31361.
    SOURCEi Search...

    Gene expression databases

    Bgeei P31361.
    CleanExi MM_POU3F3.
    Genevestigatori P31361.

    Family and domain databases

    Gene3Di 1.10.10.60. 1 hit.
    1.10.260.40. 1 hit.
    InterProi IPR017970. Homeobox_CS.
    IPR001356. Homeobox_dom.
    IPR009057. Homeodomain-like.
    IPR010982. Lambda_DNA-bd_dom.
    IPR013847. POU.
    IPR000327. POU_specific.
    IPR016362. Transcription_factor_POU.
    [Graphical view ]
    Pfami PF00046. Homeobox. 1 hit.
    PF00157. Pou. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF002629. Transcription_factor_POU. 1 hit.
    PRINTSi PR00028. POUDOMAIN.
    SMARTi SM00389. HOX. 1 hit.
    SM00352. POU. 1 hit.
    [Graphical view ]
    SUPFAMi SSF46689. SSF46689. 1 hit.
    SSF47413. SSF47413. 1 hit.
    PROSITEi PS00027. HOMEOBOX_1. 1 hit.
    PS50071. HOMEOBOX_2. 1 hit.
    PS00035. POU_1. 1 hit.
    PS00465. POU_2. 1 hit.
    PS51179. POU_3. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure and evolution of four POU domain genes expressed in mouse brain."
      Hara Y., Rovescalli C., Kim Y., Nirenberg M.
      Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "An octamer-binding site is crucial for the activity of an enhancer active at the embryonic met-/mesencephalic junction."
      Mihailescu D., Kuery P., Monard D.
      Mech. Dev. 84:55-67(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, SUBCELLULAR LOCATION.
    4. "Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons."
      Sugitani Y., Nakai S., Minowa O., Nishi M., Jishage K., Kawano H., Mori K., Ogawa M., Noda T.
      Genes Dev. 16:1760-1765(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
    5. "Transcriptional regulation of cortical neuron migration by POU domain factors."
      McEvilly R.J., de Diaz M.O., Schonemann M.D., Hooshmand F., Rosenfeld M.G.
      Science 295:1528-1532(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    6. "Replacement of related POU transcription factors leads to severe defects in mouse forebrain development."
      Wolf M., Lommes P., Sock E., Reiprich S., Friedrich R.P., Kriesch J., Stolt C.C., Bermingham J.R. Jr., Wegner M.
      Dev. Biol. 332:418-428(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPO3F3_MOUSE
    AccessioniPrimary (citable) accession number: P31361
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 27, 2011
    Last modified: October 1, 2014
    This is version 124 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    Can fully replace Pou3f1 in Schwann cells of embryonic mice and rescue peripheral nervous system development in these animals, although severe defects in forebrain development are still observed.

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3