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P31361 (PO3F3_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 120. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
POU domain, class 3, transcription factor 3
Alternative name(s):
Brain-specific homeobox/POU domain protein 1
Short name=Brain-1
Short name=Brn-1
Octamer-binding protein 8
Short name=Oct-8
Octamer-binding transcription factor 8
Short name=OTF-8
Gene names
Name:Pou3f3
Synonyms:Brn-1, Brn1, Otf8
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length497 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Transcription factor that acts synergistically with SOX11 and SOX4 By similarity. Plays a role in neuronal development. Is implicated in an enhancer activity at the embryonic met-mesencephalic junction; the enhancer element contains the octamer motif (5'-ATTTGCAT-3'). Ref.3 Ref.4 Ref.6

Subcellular location

Nucleus Ref.3.

Tissue specificity

Brain.

Developmental stage

Expressed in the developing neocortex, both in the late precursor cells and in the migrating neurons. Ref.4

Disruption phenotype

Neonatal death at least 36 hours postpartum. Loss of both Pou3f3 and Pou3f2 leads to abnormal formation of the neocortex with dramatically reduced production of layer IV-II neurons and defective migration of neurons. Ref.4 Ref.5

Miscellaneous

Can fully replace Pou3f1 in Schwann cells of embryonic mice and rescue peripheral nervous system development in these animals, although severe defects in forebrain development are still observed.

Sequence similarities

Belongs to the POU transcription factor family. Class-3 subfamily.

Contains 1 homeobox DNA-binding domain.

Contains 1 POU-specific domain.

Ontologies

Keywords
   Biological processNeurogenesis
Transcription
Transcription regulation
   Cellular componentNucleus
   DomainHomeobox
   LigandDNA-binding
   Molecular functionDevelopmental protein
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processcerebral cortex radially oriented cell migration

Inferred from genetic interaction Ref.4. Source: MGI

forebrain ventricular zone progenitor cell division

Inferred from genetic interaction Ref.4. Source: MGI

kidney development

Inferred from mutant phenotype PubMed 12925600. Source: MGI

metanephric DCT cell differentiation

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

metanephric ascending thin limb development

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

metanephric loop of Henle development

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

metanephric macula densa development

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

metanephric thick ascending limb development

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

negative regulation of apoptotic process

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

negative regulation of transcription from RNA polymerase II promoter

Inferred from sequence orthology PubMed 9632656. Source: MGI

negative regulation of transcription, DNA-templated

Inferred from direct assay PubMed 11997177. Source: UniProtKB

positive regulation of cell proliferation

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

positive regulation of transcription from RNA polymerase II promoter

Inferred from genetic interaction PubMed 17141158. Source: MGI

positive regulation of transcription, DNA-templated

Inferred from mutant phenotype PubMed 12925600. Source: UniProtKB

transcription from RNA polymerase II promoter

Inferred from sequence orthology PubMed 9632656. Source: GOC

   Cellular_componentnucleus

Inferred from sequence or structural similarity. Source: UniProtKB

   Molecular_functionDNA binding

Inferred from direct assay PubMed 15465489. Source: MGI

HMG box domain binding

Inferred from physical interaction PubMed 16582099. Source: UniProtKB

RNA polymerase II distal enhancer sequence-specific DNA binding transcription factor activity

Inferred from sequence orthology PubMed 9632656. Source: MGI

sequence-specific DNA binding

Inferred from direct assay PubMed 11997177. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 497497POU domain, class 3, transcription factor 3
PRO_0000100728

Regions

Domain311 – 38575POU-specific
DNA binding403 – 46260Homeobox
Compositional bias28 – 301274Gly-rich
Compositional bias80 – 203124Ala-rich
Compositional bias132 – 18352Pro-rich
Compositional bias269 – 30840His-rich

Experimental info

Sequence conflict101L → Q in AAA39960. Ref.1
Sequence conflict81 – 822Missing in AAA39960. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P31361 [UniParc].

Last modified July 27, 2011. Version 2.
Checksum: 4B1860BEED53F8B0

FASTA49750,200
        10         20         30         40         50         60 
MATAASNPYL PGNSLLTAGS IVHSDAAGAG GGGGGGGGGG GGAGGGGGGM QPGSAAVTSG 

        70         80         90        100        110        120 
AYRGDPSSVK MVQSDFMQGA MAASNGGHML SHAHQWVTAL PHAAAAAAAA AAAAVEASSP 

       130        140        150        160        170        180 
WSGSAVGMAG SPQQPPQPPP PPPQGPDVKG GAGREDLHAG TALHHRGPPH LGPPPPPPHQ 

       190        200        210        220        230        240 
GHPGGWGAAA AAAAAAAAAA AAAHLPSMAG GQQPPPQSLL YSQPGGFTVN GMLSAPPGPG 

       250        260        270        280        290        300 
GGGGGAGGGA QSLVHPGLVR GDTPELAEHH HHHHHHAHPH PPHPHHAQGP PHHGGGGAGP 

       310        320        330        340        350        360 
GLNSHDPHSD EDTPTSDDLE QFAKQFKQRR IKLGFTQADV GLALGTLYGN VFSQTTICRF 

       370        380        390        400        410        420 
EALQLSFKNM CKLKPLLNKW LEEADSSTGS PTSIDKIAAQ GRKRKKRTSI EVSVKGALES 

       430        440        450        460        470        480 
HFLKCPKPSA QEITNLADSL QLEKEVVRVW FCNRRQKEKR MTPPGIQQQT PDDVYSQVGT 

       490 
VSADTPPPHH GLQTSVQ 

« Hide

References

« Hide 'large scale' references
[1]"Structure and evolution of four POU domain genes expressed in mouse brain."
Hara Y., Rovescalli C., Kim Y., Nirenberg M.
Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Lineage-specific biology revealed by a finished genome assembly of the mouse."
Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S. expand/collapse author list , Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., Eichler E.E., Ponting C.P.
PLoS Biol. 7:E1000112-E1000112(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: C57BL/6J.
[3]"An octamer-binding site is crucial for the activity of an enhancer active at the embryonic met-/mesencephalic junction."
Mihailescu D., Kuery P., Monard D.
Mech. Dev. 84:55-67(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, SUBCELLULAR LOCATION.
[4]"Brn-1 and Brn-2 share crucial roles in the production and positioning of mouse neocortical neurons."
Sugitani Y., Nakai S., Minowa O., Nishi M., Jishage K., Kawano H., Mori K., Ogawa M., Noda T.
Genes Dev. 16:1760-1765(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, DISRUPTION PHENOTYPE.
[5]"Transcriptional regulation of cortical neuron migration by POU domain factors."
McEvilly R.J., de Diaz M.O., Schonemann M.D., Hooshmand F., Rosenfeld M.G.
Science 295:1528-1532(2002) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[6]"Replacement of related POU transcription factors leads to severe defects in mouse forebrain development."
Wolf M., Lommes P., Sock E., Reiprich S., Friedrich R.P., Kriesch J., Stolt C.C., Bermingham J.R. Jr., Wegner M.
Dev. Biol. 332:418-428(2009) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M88299 Genomic DNA. Translation: AAA39960.1.
AC122898 Genomic DNA. No translation available.
PIRS31223.
RefSeqNP_032926.2. NM_008900.2.
UniGeneMm.489878.

3D structure databases

ProteinModelPortalP31361.
SMRP31361. Positions 314-463.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid202307. 3 interactions.
STRING10090.ENSMUSP00000052088.

PTM databases

PhosphoSiteP31361.

Proteomic databases

PRIDEP31361.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000054883; ENSMUSP00000052088; ENSMUSG00000045515.
GeneID18993.
KEGGmmu:18993.
UCSCuc007auw.1. mouse.

Organism-specific databases

CTD5455.
MGIMGI:102564. Pou3f3.

Phylogenomic databases

eggNOGNOG261729.
GeneTreeENSGT00730000110809.
HOGENOMHOG000116303.
HOVERGENHBG053120.
InParanoidP31361.
KOK09365.
OMAAHLPSMA.
OrthoDBEOG7DJSMG.
TreeFamTF316413.

Gene expression databases

BgeeP31361.
CleanExMM_POU3F3.
GenevestigatorP31361.

Family and domain databases

Gene3D1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_specific.
IPR016362. Transcription_factor_POU.
[Graphical view]
PfamPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PIRSFPIRSF002629. Transcription_factor_POU. 1 hit.
PRINTSPR00028. POUDOMAIN.
SMARTSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio295390.
PROP31361.
SOURCESearch...

Entry information

Entry namePO3F3_MOUSE
AccessionPrimary (citable) accession number: P31361
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 27, 2011
Last modified: April 16, 2014
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot