ID PO3F2_MOUSE Reviewed; 445 AA. AC P31360; A2AJY1; DT 01-JUL-1993, integrated into UniProtKB/Swiss-Prot. DT 01-JUL-1993, sequence version 1. DT 27-MAR-2024, entry version 194. DE RecName: Full=POU domain, class 3, transcription factor 2; DE AltName: Full=Brain-specific homeobox/POU domain protein 2; DE Short=Brain-2; DE Short=Brn-2; DE AltName: Full=Nervous system-specific octamer-binding transcription factor N-Oct-3; DE AltName: Full=Octamer-binding protein 7; DE Short=Oct-7; DE AltName: Full=Octamer-binding transcription factor 7; DE Short=OTF-7; GN Name=Pou3f2; Synonyms=Brn-2, Brn2, Otf7; OS Mus musculus (Mouse). OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia; OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae; OC Murinae; Mus; Mus. OX NCBI_TaxID=10090; RN [1] RP NUCLEOTIDE SEQUENCE [GENOMIC DNA]. RC TISSUE=Brain; RX PubMed=1565620; DOI=10.1073/pnas.89.8.3280; RA Hara Y., Rovescalli C., Kim Y., Nirenberg M.; RT "Structure and evolution of four POU domain genes expressed in mouse RT brain."; RL Proc. Natl. Acad. Sci. U.S.A. 89:3280-3284(1992). RN [2] RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. RC STRAIN=C57BL/6J; RX PubMed=19468303; DOI=10.1371/journal.pbio.1000112; RA Church D.M., Goodstadt L., Hillier L.W., Zody M.C., Goldstein S., She X., RA Bult C.J., Agarwala R., Cherry J.L., DiCuccio M., Hlavina W., Kapustin Y., RA Meric P., Maglott D., Birtle Z., Marques A.C., Graves T., Zhou S., RA Teague B., Potamousis K., Churas C., Place M., Herschleb J., Runnheim R., RA Forrest D., Amos-Landgraf J., Schwartz D.C., Cheng Z., Lindblad-Toh K., RA Eichler E.E., Ponting C.P.; RT "Lineage-specific biology revealed by a finished genome assembly of the RT mouse."; RL PLoS Biol. 7:E1000112-E1000112(2009). RN [3] RP FUNCTION. RX PubMed=18505825; DOI=10.1128/mcb.00338-08; RA Hoser M., Potzner M.R., Koch J.M., Boesl M.R., Wegner M., Sock E.; RT "Sox12 deletion in the mouse reveals nonreciprocal redundancy with the RT related Sox4 and Sox11 transcription factors."; RL Mol. Cell. Biol. 28:4675-4687(2008). RN [4] RP FUNCTION. RX PubMed=18403418; DOI=10.1093/nar/gkn162; RA Dy P., Penzo-Mendez A., Wang H., Pedraza C.E., Macklin W.B., Lefebvre V.; RT "The three SoxC proteins--Sox4, Sox11 and Sox12--exhibit overlapping RT expression patterns and molecular properties."; RL Nucleic Acids Res. 36:3101-3117(2008). RN [5] RP FUNCTION. RX PubMed=20107439; DOI=10.1038/nature08797; RA Vierbuchen T., Ostermeier A., Pang Z.P., Kokubu Y., Suedhof T.C., RA Wernig M.; RT "Direct conversion of fibroblasts to functional neurons by defined RT factors."; RL Nature 463:1035-1041(2010). RN [6] RP FUNCTION. RX PubMed=24243019; DOI=10.1016/j.cell.2013.09.028; RA Wapinski O.L., Vierbuchen T., Qu K., Lee Q.Y., Chanda S., Fuentes D.R., RA Giresi P.G., Ng Y.H., Marro S., Neff N.F., Drechsel D., Martynoga B., RA Castro D.S., Webb A.E., Suedhof T.C., Brunet A., Guillemot F., Chang H.Y., RA Wernig M.; RT "Hierarchical mechanisms for direct reprogramming of fibroblasts to RT neurons."; RL Cell 155:621-635(2013). RN [7] RP INTERACTION WITH ISL1. RX PubMed=24643061; DOI=10.1371/journal.pone.0092105; RA Li R., Wu F., Ruonala R., Sapkota D., Hu Z., Mu X.; RT "Isl1 and Pou4f2 form a complex to regulate target genes in developing RT retinal ganglion cells."; RL PLoS ONE 9:E92105-E92105(2014). RN [8] RP FUNCTION. RX PubMed=27281220; DOI=10.1038/nature18323; RA Treutlein B., Lee Q.Y., Camp J.G., Mall M., Koh W., Shariati S.A., Sim S., RA Neff N.F., Skotheim J.M., Wernig M., Quake S.R.; RT "Dissecting direct reprogramming from fibroblast to neuron using single- RT cell RNA-seq."; RL Nature 534:391-395(2016). CC -!- FUNCTION: Transcription factor that plays a key role in neuronal CC differentiation (PubMed:24243019). Binds preferentially to the CC recognition sequence which consists of two distinct half-sites, CC ('GCAT') and ('TAAT'), separated by a non-conserved spacer region of 0, CC 2, or 3 nucleotides (By similarity). Acts as a transcriptional CC activator when binding cooperatively with SOX4, SOX11, or SOX12 to gene CC promoters (PubMed:18505825, PubMed:18403418). The combination of three CC transcription factors, ASCL1, POU3F2/BRN2 and MYT1L, is sufficient to CC reprogram fibroblasts and other somatic cells into induced neuronal CC (iN) cells in vitro (PubMed:20107439, PubMed:24243019, CC PubMed:27281220). Acts downstream of ASCL1, accessing chromatin that CC has been opened by ASCL1, and promotes transcription of neuronal genes CC (PubMed:24243019). {ECO:0000250|UniProtKB:P56222, CC ECO:0000269|PubMed:18403418, ECO:0000269|PubMed:18505825, CC ECO:0000269|PubMed:20107439, ECO:0000269|PubMed:24243019, CC ECO:0000269|PubMed:27281220}. CC -!- SUBUNIT: Interacts with PQBP1 (By similarity). Interaction with ISL1 CC (PubMed:24643061). {ECO:0000250|UniProtKB:P20265, CC ECO:0000269|PubMed:24643061}. CC -!- SUBCELLULAR LOCATION: Nucleus. CC -!- TISSUE SPECIFICITY: Expressed specifically in the neuroectodermal cell CC lineage. CC -!- SIMILARITY: Belongs to the POU transcription factor family. Class-3 CC subfamily. {ECO:0000305}. CC --------------------------------------------------------------------------- CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms CC Distributed under the Creative Commons Attribution (CC BY 4.0) License CC --------------------------------------------------------------------------- DR EMBL; M88300; AAA39961.1; -; Genomic_DNA. DR EMBL; AL772230; -; NOT_ANNOTATED_CDS; Genomic_DNA. DR CCDS; CCDS18005.1; -. DR PIR; S31224; S31224. DR RefSeq; NP_032925.1; NM_008899.2. DR AlphaFoldDB; P31360; -. DR SMR; P31360; -. DR BioGRID; 202306; 1. DR STRING; 10090.ENSMUSP00000136147; -. DR iPTMnet; P31360; -. DR PhosphoSitePlus; P31360; -. DR MaxQB; P31360; -. DR PaxDb; 10090-ENSMUSP00000136147; -. DR PeptideAtlas; P31360; -. DR ProteomicsDB; 289649; -. DR Antibodypedia; 18892; 417 antibodies from 42 providers. DR DNASU; 18992; -. DR Ensembl; ENSMUST00000178174.3; ENSMUSP00000136147.2; ENSMUSG00000095139.3. DR GeneID; 18992; -. DR KEGG; mmu:18992; -. DR UCSC; uc008sdp.2; mouse. DR AGR; MGI:101895; -. DR CTD; 5454; -. DR MGI; MGI:101895; Pou3f2. DR VEuPathDB; HostDB:ENSMUSG00000095139; -. DR eggNOG; KOG3802; Eukaryota. DR GeneTree; ENSGT00940000162208; -. DR HOGENOM; CLU_013065_1_2_1; -. DR InParanoid; P31360; -. DR OMA; RDAHEEP; -. DR OrthoDB; 4250502at2759; -. DR PhylomeDB; P31360; -. DR TreeFam; TF316413; -. DR BioGRID-ORCS; 18992; 0 hits in 80 CRISPR screens. DR ChiTaRS; Pou3f2; mouse. DR PRO; PR:P31360; -. DR Proteomes; UP000000589; Chromosome 4. DR RNAct; P31360; Protein. DR Bgee; ENSMUSG00000095139; Expressed in ventricular zone and 103 other cell types or tissues. DR GO; GO:0005654; C:nucleoplasm; ISO:MGI. DR GO; GO:0005634; C:nucleus; IDA:MGI. DR GO; GO:0005667; C:transcription regulator complex; IDA:MGI. DR GO; GO:0003677; F:DNA binding; IDA:UniProtKB. DR GO; GO:0001228; F:DNA-binding transcription activator activity, RNA polymerase II-specific; IDA:NTNU_SB. DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:UniProtKB. DR GO; GO:0000981; F:DNA-binding transcription factor activity, RNA polymerase II-specific; IBA:GO_Central. DR GO; GO:0071837; F:HMG box domain binding; ISO:MGI. DR GO; GO:0042802; F:identical protein binding; ISO:MGI. DR GO; GO:0000978; F:RNA polymerase II cis-regulatory region sequence-specific DNA binding; IDA:NTNU_SB. DR GO; GO:0043565; F:sequence-specific DNA binding; IDA:MGI. DR GO; GO:1990837; F:sequence-specific double-stranded DNA binding; ISO:MGI. DR GO; GO:0071310; P:cellular response to organic substance; IDA:MGI. DR GO; GO:0021799; P:cerebral cortex radially oriented cell migration; IGI:MGI. DR GO; GO:0008544; P:epidermis development; IMP:MGI. DR GO; GO:0021897; P:forebrain astrocyte development; IMP:MGI. DR GO; GO:0021869; P:forebrain ventricular zone progenitor cell division; IGI:MGI. DR GO; GO:0021979; P:hypothalamus cell differentiation; IMP:MGI. DR GO; GO:0022011; P:myelination in peripheral nervous system; IGI:MGI. DR GO; GO:0010629; P:negative regulation of gene expression; IMP:CACAO. DR GO; GO:0007399; P:nervous system development; IDA:UniProtKB. DR GO; GO:0061101; P:neuroendocrine cell differentiation; IMP:MGI. DR GO; GO:0021985; P:neurohypophysis development; IMP:MGI. DR GO; GO:0048666; P:neuron development; IDA:UniProtKB. DR GO; GO:0030182; P:neuron differentiation; IDA:UniProtKB. DR GO; GO:0048663; P:neuron fate commitment; IDA:UniProtKB. DR GO; GO:0048665; P:neuron fate specification; IDA:UniProtKB. DR GO; GO:0008284; P:positive regulation of cell population proliferation; ISO:MGI. DR GO; GO:0040018; P:positive regulation of multicellular organism growth; IMP:MGI. DR GO; GO:0045944; P:positive regulation of transcription by RNA polymerase II; IDA:UniProtKB. DR GO; GO:0050770; P:regulation of axonogenesis; IMP:MGI. DR GO; GO:0045595; P:regulation of cell differentiation; IMP:MGI. DR GO; GO:0006355; P:regulation of DNA-templated transcription; ISO:MGI. DR GO; GO:0006357; P:regulation of transcription by RNA polymerase II; IDA:UniProtKB. DR GO; GO:0014044; P:Schwann cell development; IGI:MGI. DR CDD; cd00086; homeodomain; 1. DR Gene3D; 1.10.10.60; Homeodomain-like; 1. DR Gene3D; 1.10.260.40; lambda repressor-like DNA-binding domains; 1. DR InterPro; IPR009057; Homeobox-like_sf. DR InterPro; IPR017970; Homeobox_CS. DR InterPro; IPR001356; Homeobox_dom. DR InterPro; IPR010982; Lambda_DNA-bd_dom_sf. DR InterPro; IPR013847; POU. DR InterPro; IPR000327; POU_dom. DR InterPro; IPR016362; TF_POU_3. DR PANTHER; PTHR11636; POU DOMAIN; 1. DR PANTHER; PTHR11636:SF115; POU DOMAIN, CLASS 3, TRANSCRIPTION FACTOR 2; 1. DR Pfam; PF00046; Homeodomain; 1. DR Pfam; PF00157; Pou; 1. DR PIRSF; PIRSF002629; Transcription_factor_POU; 1. DR PRINTS; PR00028; POUDOMAIN. DR SMART; SM00389; HOX; 1. DR SMART; SM00352; POU; 1. DR SUPFAM; SSF46689; Homeodomain-like; 1. DR SUPFAM; SSF47413; lambda repressor-like DNA-binding domains; 1. DR PROSITE; PS00027; HOMEOBOX_1; 1. DR PROSITE; PS50071; HOMEOBOX_2; 1. DR PROSITE; PS00035; POU_1; 1. DR PROSITE; PS00465; POU_2; 1. DR PROSITE; PS51179; POU_3; 1. DR Genevisible; P31360; MM. PE 1: Evidence at protein level; KW Activator; Differentiation; DNA-binding; Homeobox; Neurogenesis; Nucleus; KW Phosphoprotein; Reference proteome; Transcription; KW Transcription regulation. FT CHAIN 1..445 FT /note="POU domain, class 3, transcription factor 2" FT /id="PRO_0000100723" FT DOMAIN 264..338 FT /note="POU-specific" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00530" FT DNA_BIND 356..415 FT /note="Homeobox" FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00108" FT REGION 64..173 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 203..269 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT REGION 411..445 FT /note="Disordered" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 123..155 FT /note="Polar residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 214..230 FT /note="Basic and acidic residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT COMPBIAS 237..254 FT /note="Pro residues" FT /evidence="ECO:0000256|SAM:MobiDB-lite" FT MOD_RES 343 FT /note="Phosphoserine" FT /evidence="ECO:0000250|UniProtKB:P20265" SQ SEQUENCE 445 AA; 47149 MW; 1A47F10950EECE8A CRC64; MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ QRPPHLVHHA ANHHPGPGAW RSAAAAAHLP PSMGASNGGL LYSQPSFTVN GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ PPPPPPPQGP PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI AAQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV RVWFCNRRQK EKRMTPPGGT LPGAEDVYGG SRDTPPHHGV QTPVQ //