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Protein

POU domain, class 3, transcription factor 2

Gene

Pou3f2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Transcription factor that binds preferentially to the recognition sequence which consists of two distinct half-sites, ('GCAT') and ('TAAT'), separated by a nonconserved spacer region of 0, 2, or 3 nucleotides. Positively regulates the genes under the control of corticotropin-releasing hormone (CRH) and CRH II promoters (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
DNA bindingi356 – 415HomeoboxPROSITE-ProRule annotationAdd BLAST60

GO - Molecular functioni

  • DNA binding Source: MGI
  • RNA polymerase II core promoter proximal region sequence-specific DNA binding Source: NTNU_SB
  • RNA polymerase II transcription coactivator activity Source: UniProtKB
  • sequence-specific DNA binding Source: MGI
  • transcriptional activator activity, RNA polymerase II core promoter proximal region sequence-specific binding Source: NTNU_SB

GO - Biological processi

  • astrocyte development Source: MGI
  • cellular response to organic substance Source: MGI
  • cerebral cortex radially oriented cell migration Source: MGI
  • epidermis development Source: MGI
  • forebrain ventricular zone progenitor cell division Source: MGI
  • hypothalamus cell differentiation Source: MGI
  • myelination in peripheral nervous system Source: MGI
  • negative regulation of gene expression Source: CACAO
  • neurohypophysis development Source: MGI
  • neuron differentiation Source: MGI
  • positive regulation of cell proliferation Source: MGI
  • positive regulation of multicellular organism growth Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: NTNU_SB
  • regulation of axonogenesis Source: MGI
  • regulation of cell differentiation Source: MGI
  • regulation of transcription from RNA polymerase II promoter Source: MGI
  • Schwann cell development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Activator

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
POU domain, class 3, transcription factor 2
Alternative name(s):
Brain-specific homeobox/POU domain protein 2
Short name:
Brain-2
Short name:
Brn-2
Nervous system-specific octamer-binding transcription factor N-Oct-3
Octamer-binding protein 7
Short name:
Oct-7
Octamer-binding transcription factor 7
Short name:
OTF-7
Gene namesi
Name:Pou3f2
Synonyms:Brn-2, Brn2, Otf7
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:101895. Pou3f2.

Subcellular locationi

GO - Cellular componenti

  • nucleus Source: MGI
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001007231 – 445POU domain, class 3, transcription factor 2Add BLAST445

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei343PhosphoserineBy similarity1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31360.
PaxDbiP31360.
PeptideAtlasiP31360.
PRIDEiP31360.

PTM databases

iPTMnetiP31360.
PhosphoSitePlusiP31360.

Expressioni

Tissue specificityi

Expressed specifically in the neuroectodermal cell lineage.

Gene expression databases

BgeeiENSMUSG00000095139.
CleanExiMM_POU3F2.
GenevisibleiP31360. MM.

Interactioni

Subunit structurei

Interacts with PQBP1.By similarity

Protein-protein interaction databases

BioGridi202306. 1 interactor.
STRINGi10090.ENSMUSP00000136147.

Structurei

3D structure databases

ProteinModelPortaliP31360.
SMRiP31360.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini264 – 338POU-specificPROSITE-ProRule annotationAdd BLAST75

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi68 – 90Poly-GlyAdd BLAST23
Compositional biasi125 – 149Poly-GlnAdd BLAST25

Sequence similaritiesi

Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation
Contains 1 POU-specific domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP31360.
KOiK09365.
OMAiHPASQHQ.
OrthoDBiEOG091G0U0S.
PhylomeDBiP31360.
TreeFamiTF316413.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31360-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAASNHYS LLTSSASIVH AEPPGGMQQG AGGYREAQSL VQGDYGALQS
60 70 80 90 100
NGHPLSHAHQ WITALSHGGG GGGGGGGGGG GGGGGGGGDG SPWSTSPLGQ
110 120 130 140 150
PDIKPSVVVQ QGGRGDELHG PGALQQQHQQ QQQQQQQQQQ QQQQQQQQQQ
160 170 180 190 200
QRPPHLVHHA ANHHPGPGAW RSAAAAAHLP PSMGASNGGL LYSQPSFTVN
210 220 230 240 250
GMLGAGGQPA GLHHHGLRDA HDEPHHADHH PHPHSHPHQQ PPPPPPPQGP
260 270 280 290 300
PGHPGAHHDP HSDEDTPTSD DLEQFAKQFK QRRIKLGFTQ ADVGLALGTL
310 320 330 340 350
YGNVFSQTTI CRFEALQLSF KNMCKLKPLL NKWLEEADSS SGSPTSIDKI
360 370 380 390 400
AAQGRKRKKR TSIEVSVKGA LESHFLKCPK PSAQEITSLA DSLQLEKEVV
410 420 430 440
RVWFCNRRQK EKRMTPPGGT LPGAEDVYGG SRDTPPHHGV QTPVQ
Length:445
Mass (Da):47,149
Last modified:July 1, 1993 - v1
Checksum:i1A47F10950EECE8A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88300 Genomic DNA. Translation: AAA39961.1.
AL772230 Genomic DNA. Translation: CAM22634.1.
CCDSiCCDS18005.1.
PIRiS31224.
RefSeqiNP_032925.1. NM_008899.2.
UniGeneiMm.129387.
Mm.389520.
Mm.487875.

Genome annotation databases

EnsembliENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139.
GeneIDi18992.
KEGGimmu:18992.
UCSCiuc008sdp.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M88300 Genomic DNA. Translation: AAA39961.1.
AL772230 Genomic DNA. Translation: CAM22634.1.
CCDSiCCDS18005.1.
PIRiS31224.
RefSeqiNP_032925.1. NM_008899.2.
UniGeneiMm.129387.
Mm.389520.
Mm.487875.

3D structure databases

ProteinModelPortaliP31360.
SMRiP31360.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi202306. 1 interactor.
STRINGi10090.ENSMUSP00000136147.

PTM databases

iPTMnetiP31360.
PhosphoSitePlusiP31360.

Proteomic databases

MaxQBiP31360.
PaxDbiP31360.
PeptideAtlasiP31360.
PRIDEiP31360.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000178174; ENSMUSP00000136147; ENSMUSG00000095139.
GeneIDi18992.
KEGGimmu:18992.
UCSCiuc008sdp.2. mouse.

Organism-specific databases

CTDi5454.
MGIiMGI:101895. Pou3f2.

Phylogenomic databases

eggNOGiKOG3802. Eukaryota.
ENOG410XQ7X. LUCA.
GeneTreeiENSGT00760000118935.
HOGENOMiHOG000116303.
HOVERGENiHBG053120.
InParanoidiP31360.
KOiK09365.
OMAiHPASQHQ.
OrthoDBiEOG091G0U0S.
PhylomeDBiP31360.
TreeFamiTF316413.

Miscellaneous databases

PROiP31360.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000095139.
CleanExiMM_POU3F2.
GenevisibleiP31360. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
1.10.260.40. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR009057. Homeodomain-like.
IPR010982. Lambda_DNA-bd_dom.
IPR013847. POU.
IPR000327. POU_dom.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
PF00157. Pou. 1 hit.
[Graphical view]
PRINTSiPR00028. POUDOMAIN.
SMARTiSM00389. HOX. 1 hit.
SM00352. POU. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
SSF47413. SSF47413. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
PS00035. POU_1. 1 hit.
PS00465. POU_2. 1 hit.
PS51179. POU_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPO3F2_MOUSE
AccessioniPrimary (citable) accession number: P31360
Secondary accession number(s): A2AJY1
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 150 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.