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P31356 (OPSD_TODPA) Reviewed, UniProtKB/Swiss-Prot

Last modified October 19, 2011. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Rhodopsin
Gene names
Name:RHO
OrganismTodarodes pacificus (Japanese flying squid) (Ommastrephes pacificus)
Taxonomic identifier6637 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Mollusca › Cephalopoda › Coleoidea › Neocoleoidea › Decapodiformes › Teuthida › Oegopsina › Ommastrephidae › Todarodes

Protein attributes

Sequence length448 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Visual pigments such as rhodopsin and porphyropsin are light-absorbing molecules that mediate vision. Rhodopsin consists of an apoprotein, opsin, covalently linked to 11-cis-retinal. This receptor is coupled to the activation of phospholipase C. Porphyropsin consists of opsin covalently linked to 11-cis 3,4-didehydroretinal.

Subcellular location

Membrane; Multi-pass membrane protein.

Post-translational modification

Phosphorylated on some or all of the serine and threonine residues present in the C-terminal region.

Sequence similarities

Belongs to the G-protein coupled receptor 1 family. Opsin subfamily.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed Ref.1
Chain2 – 448447Rhodopsin
PRO_0000197748

Regions

Topological domain2 – 3332Extracellular Potential
Transmembrane34 – 5825Helical; Name=1; Potential
Topological domain59 – 7012Cytoplasmic Potential
Transmembrane71 – 9727Helical; Name=2; Potential
Topological domain98 – 11114Extracellular Potential
Transmembrane112 – 13120Helical; Name=3; Potential
Topological domain132 – 15120Cytoplasmic Potential
Transmembrane152 – 17524Helical; Name=4; Potential
Topological domain176 – 19924Extracellular Potential
Transmembrane200 – 22728Helical; Name=5; Potential
Topological domain228 – 26134Cytoplasmic Potential
Transmembrane262 – 28524Helical; Name=6; Potential
Topological domain286 – 2938Extracellular Potential
Transmembrane294 – 31825Helical; Name=7; Potential
Topological domain319 – 448130Cytoplasmic Potential
Compositional bias371 – 38111Met-rich
Compositional bias382 – 44867Gln/Pro-rich

Amino acid modifications

Modified residue3051N6-(retinylidene)lysine
Lipidation3361S-palmitoyl cysteine By similarity
Lipidation3371S-palmitoyl cysteine By similarity
Glycosylation81N-linked (GlcNAc...)
Disulfide bond108 ↔ 186 By similarity

Secondary structure

1 .............................................. 448
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P31356 [UniParc].

Last modified January 23, 2007. Version 2.
Checksum: B48D56A1D057492A

FASTA44849,836
        10         20         30         40         50         60 
MGRDLRDNET WWYNPSIVVH PHWREFDQVP DAVYYSLGIF IGICGIIGCG GNGIVIYLFT 

        70         80         90        100        110        120 
KTKSLQTPAN MFIINLAFSD FTFSLVNGFP LMTISCFLKK WIFGFAACKV YGFIGGIFGF 

       130        140        150        160        170        180 
MSIMTMAMIS IDRYNVIGRP MAASKKMSHR RAFIMIIFVW LWSVLWAIGP IFGWGAYTLE 

       190        200        210        220        230        240 
GVLCNCSFDY ISRDSTTRSN ILCMFILGFF GPILIIFFCY FNIVMSVSNH EKEMAAMAKR 

       250        260        270        280        290        300 
LNAKELRKAQ AGANAEMRLA KISIVIVSQF LLSWSPYAVV ALLAQFGPLE WVTPYAAQLP 

       310        320        330        340        350        360 
VMFAKASAIH NPMIYSVSHP KFREAISQTF PWVLTCCQFD DKETEDDKDA ETEIPAGESS 

       370        380        390        400        410        420 
DAAPSADAAQ MKEMMAMMQK MQQQQAAYPP QGYAPPPQGY PPQGYPPQGY PPQGYPPQGY 

       430        440 
PPPPQGAPPQ GAPPAAPPQG VDNQAYQA

« Hide

References

[1]"Cloning and nucleotide sequence of cDNA for rhodopsin of the squid Todarodes pacificus."
Hara-Nishimura I., Kondo M., Nishimura M., Hara R., Hara T.
FEBS Lett. 317:5-11(1993) [PubMed: 8428633] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 2-17; 129-140 AND 303-313.
Tissue: Retina.
[2]"Amino acid sequence of the retinal binding site of squid visual pigment."
Seidou M., Kubota I., Hiraki K., Kito Y.
Biochim. Biophys. Acta 957:318-321(1988) [PubMed: 3191148] [Abstract]
Cited for: PROTEIN SEQUENCE OF 303-313, RETINAL-CHROMOPHORE BINDING AT LYS-305.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		X70498 mRNA. Translation: CAA49906.1.
PIRS29483.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2Z73X-ray2.50A/B1-448[»]
2ZIYX-ray3.70A2-373[»]
3AYMX-ray2.80A/B1-448[»]
3AYNX-ray2.70A/B1-448[»]
ProteinModelPortalP31356.
SMRP31356. Positions 9-358.
ModBaseSearch...

Protein family/group databases

GPCRDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR000276. 7TM_GPCR_Rhodpsn.
IPR017452. GPCR_Rhodpsn_supfam.
IPR001760. Opsin.
IPR006031. XYPPX.
[Graphical view]
PfamPF00001. 7tm_1. 1 hit.
PF02162. XYPPX. 5 hits.
[Graphical view]
PRINTSPR00237. GPCRRHODOPSN.
PR00238. OPSIN.
PROSITEPS00237. G_PROTEIN_RECEP_F1_1. 1 hit.
PS50262. G_PROTEIN_RECEP_F1_2. 1 hit.
PS00238. OPSIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameOPSD_TODPA
AccessionPrimary (citable) accession number: P31356
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: October 19, 2011
This is version 71 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)

Relevant documents

7-transmembrane G-linked receptors

List of 7-transmembrane G-linked receptor entries

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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