P31352 (L_MABVM) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Lake Victoria marburgvirus (strain Musoke-80) (MARV) (Marburg virus (strain Kenya/Musoke/1980))

Taxonomic identifier

33727 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Filoviridae › Marburgvirus

Virus host

Rousettus aegyptiacus (Egyptian rousette) (Egyptian fruit bat) [TaxID: 9407]
Chlorocebus aethiops (Green monkey) (Cercopithecus aethiops) [TaxID: 9534]
Homo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length	2331 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subcellular location	Host cytoplasm. Virion By similarity.
Sequence similarities	Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme Reference proteome
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2331	2331	RNA-directed RNA polymerase L		PRO_0000222169
Regions
	Domain	628 – 812	185	RdRp catalytic
Natural variations
	Natural variant	489	1	L → A in strain: pp3/guinea pig lethal, pp4/guinea pig nonlethal and Isolate Enterlein.
	Natural variant	741	1	S → C in strain: pp3/guinea pig lethal.
	Natural variant	979	1	R → G in strain: pp3/guinea pig lethal, pp4/guinea pig nonlethal and Isolate Enterlein.
	Natural variant	1428	1	S → T in strain: Isolate Enterlein.

Sequences

	Sequence		Length	Mass (Da)
	P31352 [UniParc]. Last modified February 1, 1996. Version 2. Checksum: 1D54C60DA1BED3CF	FASTA	2,331	267,093
	10 20 30 40 50 60 MQHPTQYPDA RLSSPIILDQ CDLLARSLGL YSHYSHNPKL RNCRIPHHIY RLRNSTALKT 70 80 90 100 110 120 FLQNCSILTV PFHSIWDHIL TSIQYDAINH VDDFKYLLPS ELVKYANWDN EFLKAYLNKI 130 140 150 160 170 180 LGLDHVFSAS ARSQCEDFSP KENPYYWGML LLVHLSQLAR RIKGQRGSLR SNWKFIGTDL 190 200 210 220 230 240 ELFGIADFVI FKVPVKTIIR NAVSLQASKP GLRIWYRDQN LTPYLCDDEF IVSVASYECF 250 260 270 280 290 300 IMIKDVFIER YNTWEICARA WLEDSDGADY PPLDVLGELY NQGDQIIAMY LEDGFKLIKH 310 320 330 340 350 360 LEPLCVSCIQ THGIFTPRKY WFQSQMIKSY YDELHDLNLK LQISDNKAEC AQNFIKTIVQ 370 380 390 400 410 420 AKLTPQQYCE LFSLQKHWGH PVLYNDVALD KVKKHAQSTK ILKPKVMFET FCVFKFIVAK 430 440 450 460 470 480 NHYHSQGSWY KTTHDLHLTP YLRQHIVSNS FPSQAEIYQH LWEWYFVEHE PLFSTKIISD 490 500 510 520 530 540 LSIFIKDRLT AVNQECWDSV FDRSVLGYNP PVRFQSKRVP EQFLGQADFS LNQILEFAEK 550 560 570 580 590 600 LEYLAPSYRN FSFSLKEKEL NIGRTFGKLP YRVRNVQTLA EALLADGLAK AFPSNMMVVT 610 620 630 640 650 660 EREQKEALLH QASWHHNSAS IGENAIVRGA SFVTDLEKYN LAFRYEFTRH FIDYCNRCYG 670 680 690 700 710 720 VKNLFDWMHF LIPLCYMHVS DFYSPPHCVT EDNRNNPPDC ANAYHYHLGG IEGLQQKLWT 730 740 750 760 770 780 CISCAQITLV ELKTKLKLKS SVMGDNQCIT TLSLFPIDAP NDYQENEAEL NAARVAVELA 790 800 810 820 830 840 ITTGYSGIFL KPEETFVHSG FIYFGKKQYL NGVQLPQSLK TMARCGPLSD SIFDDLQGSL 850 860 870 880 890 900 ASIGTSFERG TSETRHIFPS RWIASFHSML AINLLNQNHL GFPLGFNIDI SCFKKPLTFS 910 920 930 940 950 960 EKLIALITPQ VLGGLSFLNP EKLFYRNISD PLTSGLFQLK NALEFLEKEE LFYILISKKP 970 980 990 1000 1010 1020 GLADASDFVM NPLGLNVPRS KEIITFLRQT VRENITITSQ NRIINSLFHI GSDLEDQRVC 1030 1040 1050 1060 1070 1080 EWLLSSNPVM SRFAADIFSR TPSGKRLQVL GYLEGTRTLL ASRTISLTTE GTMLMKLREL 1090 1100 1110 1120 1130 1140 TRNRWKSWFS YIDALDDDLS ESLEKFTCTV DVANFLRAYS WSDVLKGKRL IGATLPCLLE 1150 1160 1170 1180 1190 1200 QFEVKWINLS EDLREQFNLS SDSKSTINLL PYDCKELRLE GSNDTELNYV SCALDRKVVQ 1210 1220 1230 1240 1250 1260 KHPSVNRLAW TIGNRAPYIG SRTEDKIGYP PLRVNCPSAA LKEAIEMVSR LLWVTQGTAD 1270 1280 1290 1300 1310 1320 REKLLIPLLN SRVNLDYQTV LNFLPTHYSG NIVHRYNDQY GQHSFMANRM SNTSTRAIIS 1330 1340 1350 1360 1370 1380 TNTLGKYAGG GQAAIDSNII FQNTINLGVA VLDIALSLAK LSSASNVTFR LMLNKCCTRH 1390 1400 1410 1420 1430 1440 VPSEYLYFDK PLDVDLNKYM DNELVYDNDP LCSGIKGRLG RVSRSTLSLS LNVSDIGSYD 1450 1460 1470 1480 1490 1500 FPTIAAWTLG ETIVGSIFSD ESSQSTDPIS SGCTKTFVTH FLVYPVESIF YAFGANLIVE 1510 1520 1530 1540 1550 1560 SLSLSRIKSI KNLSDLTFLI SSTIRNLSHR SLRILQSTFR HELVLTRLAH HIPLISLMLG 1570 1580 1590 1600 1610 1620 GSAGEKSSSD AVRLFLTASY QNFINNFSCL MKKGQSSLPV WLYFPSEGQQ LKPILKILQR 1630 1640 1650 1660 1670 1680 LSDLLSPDKI QKRKILADTC CPIGSFWVYP SKSTRTNHYY ASLNYWRDKA NKVKNTPFSH 1690 1700 1710 1720 1730 1740 LINCSFPEFS SHTSSVSSNQ QVTNSKYIVY PENITEINAR TRLINYGSTA LQGMDTKMPL 1750 1760 1770 1780 1790 1800 SEQNLVENCR PSEGIRFKDN QKITKHDQRC EREESSPQQM FPEDNMQTPA HIHSSSPFQI 1810 1820 1830 1840 1850 1860 LIKSLDAHED FDASKIILNS EINNLNLTEY TLNTKLLTTP TRTEILDTSP LQSSRYSSTS 1870 1880 1890 1900 1910 1920 RERSLLSREQ ASYLYVDCSN IPSISLDPGF RSMSDQNQVQ MLINTYKRDL HACFDSNQFC 1930 1940 1950 1960 1970 1980 RFTGVVSSMH YKLYDLLPPG KLKKAICLAE GEGSGARLLL KWKETDYLFF NTLATDSQQE 1990 2000 2010 2020 2030 2040 AEILSGRVIP RMLYNIDRLS ALLESRRLIL NNLTIQITDI TNPLWLDSVI QYLPEDSDIL 2050 2060 2070 2080 2090 2100 TMDAETTKDE TREQLYKTIV NIWTRTSPNI PKISIIKVFL LDYEGTLFLM KNAIQYYGQV 2110 2120 2130 2140 2150 2160 QLKKPYSSNA KNSEWYLCCG KRRIQRLQID FSDQVGIFLI CKAMSRQRQA IPYWLKHIEK 2170 2180 2190 2200 2210 2220 NYPASLHEFF LTLGFPSLES SFCHRYTIPF SEGKALFHKV QSYVRQGKQH LHSLMLDYEN 2230 2240 2250 2260 2270 2280 NSPLLDLRNH FICSLRGKIT KYYNDILKLN LVIKAVEKGK NWSQLVEILP NMHSVCIVHV 2290 2300 2310 2320 2330 DHECSGCEKR LLLKLDFIRN TKIAEQKLLN RVIGYILFFP FGLFKSGSLR A

References

[1]	"The nucleotide sequence of the L gene of Marburg virus, a filovirus: homologies with paramyxoviruses and rhabdoviruses." Muehlberger E., Sanchez A., Randolf A., Will C., Kiley M.P., Klenk H.-D., Feldmann H. Virology 187:534-547(1992) [PubMed: 1546452] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA].
[2]	Feldmann H. Submitted (SEP-1994) to the EMBL/GenBank/DDBJ databases Cited for: SEQUENCE REVISION.
[3]	Chain P.S.G., Malfatti S.A., Hajjaj A., Vergez L.M., Do L.H., Smith K.L., McCready P.M. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
[4]	Ichou M.A., Paragas J., Jahrling P.B., Ibrahim M.S., Lofts L., Hevey M., Schmaljohn A. Submitted (OCT-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: pp3/guinea pig lethal and pp4/guinea pig nonlethal.
[5]	"Rescue of recombinant Marburg virus from cDNA is dependent on nucleocapsid protein VP30." Enterlein S., Volchkov V., Weik M., Kolesnikova L., Volchkova V., Klenk H.-D., Muehlberger E. J. Virol. 80:1038-1043(2006) [PubMed: 16379005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC RNA]. Strain: Isolate Enterlein.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M92834 Genomic RNA. Translation: AAA46562.1. Sequence problems. Z12132 Genomic DNA. Translation: CAA78120.1. AY430365 Genomic RNA. Translation: AAR85466.1. AY430366 Genomic RNA. Translation: AAR85459.1. DQ217792 Genomic RNA. Translation: ABA87130.1.
PIR	RRIWMV. A42450.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. IPR017235. RNA-dir_pol_filovirus. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PIRSF	PIRSF037548. RNA_pol_Filoviridae. 1 hit.
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_MABVM

Accession

Primary (citable) accession number: P31352
Secondary accession number(s): Q38L39, Q6T6T7, Q6T6U4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	February 1, 1996
Last modified:	December 14, 2011
This is version 69 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program