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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Victoria/3/1975 H3N2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with the host mitochondrial antiviral signaling protein MAVS.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2
Alternative name(s):
RNA-directed RNA polymerase subunit P3
Gene namesi
Name:PB2
OrganismiInfluenza A virus (strain A/Victoria/3/1975 H3N2)
Taxonomic identifieri392809 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Cetacea (whales) [TaxID: 9721]
Homo sapiens (Human) [TaxID: 9606]
Phocidae (true seals) [TaxID: 9709]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Virion
  • Host nucleus By similarity
  • Host mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000788401 – 759Polymerase basic protein 2Add BLAST759

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.By similarity

Protein-protein interaction databases

DIPiDIP-29297N.
IntActiP31345. 3 interactors.

Structurei

Secondary structure

1759
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi322 – 325Combined sources4
Beta strandi328 – 335Combined sources8
Beta strandi338 – 345Combined sources8
Beta strandi351 – 359Combined sources9
Beta strandi361 – 366Combined sources6
Beta strandi368 – 377Combined sources10
Beta strandi380 – 390Combined sources11
Helixi391 – 404Combined sources14
Helixi408 – 411Combined sources4
Helixi423 – 425Combined sources3
Helixi430 – 440Combined sources11
Helixi443 – 448Combined sources6
Beta strandi451 – 453Combined sources3
Beta strandi460 – 463Combined sources4
Beta strandi469 – 475Combined sources7
Beta strandi478 – 481Combined sources4
Helixi541 – 555Combined sources15
Helixi557 – 566Combined sources10
Helixi568 – 572Combined sources5
Helixi575 – 577Combined sources3
Helixi578 – 583Combined sources6
Turni586 – 588Combined sources3
Helixi589 – 607Combined sources19
Helixi612 – 618Combined sources7
Helixi619 – 622Combined sources4
Beta strandi634 – 638Combined sources5
Beta strandi645 – 651Combined sources7
Beta strandi654 – 659Combined sources6
Turni660 – 663Combined sources4
Beta strandi664 – 667Combined sources4
Beta strandi670 – 674Combined sources5
Helixi688 – 691Combined sources4
Beta strandi694 – 699Combined sources6
Helixi702 – 704Combined sources3
Helixi710 – 713Combined sources4
Beta strandi721 – 726Combined sources6
Turni727 – 729Combined sources3
Beta strandi730 – 738Combined sources9
Turni745 – 747Combined sources3
Beta strandi751 – 753Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GMONMR-A678-759[»]
2JDQX-ray2.20D/E678-759[»]
2VQZX-ray2.30A/B/D/E/F318-483[»]
2VY6X-ray1.95A538-753[»]
2VY7X-ray1.53A538-693[»]
2VY8X-ray1.20A538-693[»]
4NCEX-ray2.30A318-483[»]
4NCMX-ray2.82A318-483[»]
4P1UX-ray2.52A318-483[»]
4UADX-ray2.42E683-759[»]
4UAEX-ray2.70F678-759[»]
4UAFX-ray1.70E678-759[»]
ProteinModelPortaliP31345.
SMRiP31345.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31345.

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi736 – 739Nuclear localization signalBy similarity4

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.Curated

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P31345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPSLRMKWM
60 70 80 90 100
MAMKYPITAD KRITEMVPER NEQGQTLWSK MSDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPVTSTVH YPKVYKTYFD KVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV
260 270 280 290 300
RNDDIDQSLI IAARNIVRRA SVSADPLASL LEMCHSTQIG GTRMVDILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSIKR EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGKRA TAILRKATRR LVQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG
460 470 480 490 500
IEHIDNVMGM VGVLPDMTPS TEMSMRGIRV SKMGVDEYSS TERVVVSIDR
510 520 530 540 550
FLRVRDQRGN VLLSPEEVSE THGTERLTIT YSSSMMWEIN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTTQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILVR
660 670 680 690 700
GNSPVFNYNK TTKRLTILGK DAGTLIEDPD ESTSGVESAV LRGFLILGKE
710 720 730 740 750
DRRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):86,125
Last modified:July 1, 1993 - v1
Checksum:i22DD5C3A573DA870
GO

Sequence databases

PIRiB60008.

Cross-referencesi

Sequence databases

PIRiB60008.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2GMONMR-A678-759[»]
2JDQX-ray2.20D/E678-759[»]
2VQZX-ray2.30A/B/D/E/F318-483[»]
2VY6X-ray1.95A538-753[»]
2VY7X-ray1.53A538-693[»]
2VY8X-ray1.20A538-693[»]
4NCEX-ray2.30A318-483[»]
4NCMX-ray2.82A318-483[»]
4P1UX-ray2.52A318-483[»]
4UADX-ray2.42E683-759[»]
4UAEX-ray2.70F678-759[»]
4UAFX-ray1.70E678-759[»]
ProteinModelPortaliP31345.
SMRiP31345.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29297N.
IntActiP31345. 3 interactors.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP31345.

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiPB2_I75A3
AccessioniPrimary (citable) accession number: P31345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.