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Protein

Polymerase basic protein 2

Gene

PB2

Organism
Influenza A virus (strain A/Victoria/3/1975 H3N2)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Plays an essential role in transcription initiation and cap-stealing mechanism, in which cellular capped pre-mRNAs are used to generate primers for viral transcription. Binds the cap of the target pre-RNA which is subsequently cleaved after 10-13 nucleotides by PA. Plays a role in the initiation of the viral genome replication and modulates the activity of the ribonucleoprotein (RNP) complex. In addition, participates in the inhibition of type I interferon induction through interaction with the host mitochondrial antiviral signaling protein MAVS.By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Cap snatching, Eukaryotic host gene expression shutoff by virus, Eukaryotic host transcription shutoff by virus, Host gene expression shutoff by virus, Host-virus interaction, Inhibition of host innate immune response by virus, Inhibition of host MAVS by virus, Inhibition of host RLR pathway by virus, Inhibition of host RNA polymerase II by virus, mRNA capping, mRNA processing, Viral immunoevasion, Viral transcription

Names & Taxonomyi

Protein namesi
Recommended name:
Polymerase basic protein 2
Alternative name(s):
RNA-directed RNA polymerase subunit P3
Gene namesi
Name:PB2
OrganismiInfluenza A virus (strain A/Victoria/3/1975 H3N2)
Taxonomic identifieri392809 [NCBI]
Taxonomic lineageiVirusesssRNA virusesssRNA negative-strand virusesOrthomyxoviridaeInfluenzavirus A
Virus hostiAves [TaxID: 8782]
Cetacea (whales) [TaxID: 9721]
Homo sapiens (Human) [TaxID: 9606]
Phocidae (true seals) [TaxID: 9709]
Sus scrofa (Pig) [TaxID: 9823]

Subcellular locationi

  • Virion
  • Host nucleus By similarity
  • Host mitochondrion By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host mitochondrion, Host nucleus, Virion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 759759Polymerase basic protein 2PRO_0000078840Add
BLAST

Interactioni

Subunit structurei

Influenza RNA polymerase is composed of three subunits: PB1, PB2 and PA. Interacts (via N-terminus) with PB1 (via C-terminus). Interacts with nucleoprotein NP (via N-terminus). Interacts (via N-terminus) with host MAVS (via N-terminus); this interaction inhibits host innate immune response.By similarity

Protein-protein interaction databases

DIPiDIP-29297N.
IntActiP31345. 3 interactions.

Structurei

Secondary structure

1
759
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi322 – 3254Combined sources
Beta strandi328 – 3358Combined sources
Beta strandi338 – 3458Combined sources
Beta strandi351 – 3599Combined sources
Beta strandi361 – 3666Combined sources
Beta strandi368 – 37710Combined sources
Beta strandi380 – 39011Combined sources
Helixi391 – 40414Combined sources
Helixi408 – 4114Combined sources
Helixi423 – 4253Combined sources
Helixi430 – 44011Combined sources
Helixi443 – 4486Combined sources
Beta strandi451 – 4533Combined sources
Beta strandi460 – 4634Combined sources
Beta strandi469 – 4757Combined sources
Beta strandi478 – 4814Combined sources
Helixi541 – 55515Combined sources
Helixi557 – 56610Combined sources
Helixi568 – 5725Combined sources
Helixi575 – 5773Combined sources
Helixi578 – 5836Combined sources
Turni586 – 5883Combined sources
Helixi589 – 60719Combined sources
Helixi612 – 6187Combined sources
Helixi619 – 6224Combined sources
Beta strandi634 – 6385Combined sources
Beta strandi645 – 6517Combined sources
Beta strandi654 – 6596Combined sources
Turni660 – 6634Combined sources
Beta strandi664 – 6674Combined sources
Beta strandi670 – 6745Combined sources
Helixi688 – 6914Combined sources
Beta strandi694 – 6996Combined sources
Helixi702 – 7043Combined sources
Helixi710 – 7134Combined sources
Beta strandi721 – 7266Combined sources
Turni727 – 7293Combined sources
Beta strandi730 – 7389Combined sources
Turni745 – 7473Combined sources
Beta strandi751 – 7533Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GMONMR-A678-759[»]
2JDQX-ray2.20D/E678-759[»]
2VQZX-ray2.30A/B/D/E/F318-483[»]
2VY6X-ray1.95A538-753[»]
2VY7X-ray1.53A538-693[»]
2VY8X-ray1.20A538-693[»]
4NCEX-ray2.30A318-483[»]
4NCMX-ray2.82A318-483[»]
4P1UX-ray2.52A318-483[»]
4UADX-ray2.42E683-759[»]
4UAEX-ray2.70F678-759[»]
4UAFX-ray1.70E678-759[»]
ProteinModelPortaliP31345.
SMRiP31345. Positions 319-483, 685-759.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31345.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi736 – 7394Nuclear localization signalBy similarity

Sequence similaritiesi

Belongs to the influenza viruses PB2 family.Curated

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]

Sequencei

Sequence statusi: Complete.

P31345-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MERIKELRNL MSQSRTREIL TKTTVDHMAI IKKYTSGRQE KNPSLRMKWM
60 70 80 90 100
MAMKYPITAD KRITEMVPER NEQGQTLWSK MSDAGSDRVM VSPLAVTWWN
110 120 130 140 150
RNGPVTSTVH YPKVYKTYFD KVERLKHGTF GPVHFRNQVK IRRRVDINPG
160 170 180 190 200
HADLSAKEAQ DVIMEVVFPN EVGARILTSE SQLTITKEKK EELQDCKISP
210 220 230 240 250
LMVAYMLERE LVRKTRFLPV AGGTSSVYIE VLHLTQGTCW EQMYTPGGEV
260 270 280 290 300
RNDDIDQSLI IAARNIVRRA SVSADPLASL LEMCHSTQIG GTRMVDILRQ
310 320 330 340 350
NPTEEQAVDI CKAAMGLRIS SSFSFGGFTF KRTSGSSIKR EEEVLTGNLQ
360 370 380 390 400
TLKIRVHEGY EEFTMVGKRA TAILRKATRR LVQLIVSGRD EQSIAEAIIV
410 420 430 440 450
AMVFSQEDCM IKAVRGDLNF VNRANQRLNP MHQLLRHFQK DAKVLFQNWG
460 470 480 490 500
IEHIDNVMGM VGVLPDMTPS TEMSMRGIRV SKMGVDEYSS TERVVVSIDR
510 520 530 540 550
FLRVRDQRGN VLLSPEEVSE THGTERLTIT YSSSMMWEIN GPESVLVNTY
560 570 580 590 600
QWIIRNWETV KIQWSQNPTM LYNKMEFEPF QSLVPKAIRG QYSGFVRTLF
610 620 630 640 650
QQMRDVLGTF DTTQIIKLLP FAAAPPKQSR MQFSSLTVNV RGSGMRILVR
660 670 680 690 700
GNSPVFNYNK TTKRLTILGK DAGTLIEDPD ESTSGVESAV LRGFLILGKE
710 720 730 740 750
DRRYGPALSI NELSNLAKGE KANVLIGQGD VVLVMKRKRD SSILTDSQTA

TKRIRMAIN
Length:759
Mass (Da):86,125
Last modified:July 1, 1993 - v1
Checksum:i22DD5C3A573DA870
GO

Sequence databases

PIRiB60008.

Cross-referencesi

Sequence databases

PIRiB60008.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2GMONMR-A678-759[»]
2JDQX-ray2.20D/E678-759[»]
2VQZX-ray2.30A/B/D/E/F318-483[»]
2VY6X-ray1.95A538-753[»]
2VY7X-ray1.53A538-693[»]
2VY8X-ray1.20A538-693[»]
4NCEX-ray2.30A318-483[»]
4NCMX-ray2.82A318-483[»]
4P1UX-ray2.52A318-483[»]
4UADX-ray2.42E683-759[»]
4UAEX-ray2.70F678-759[»]
4UAFX-ray1.70E678-759[»]
ProteinModelPortaliP31345.
SMRiP31345. Positions 319-483, 685-759.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-29297N.
IntActiP31345. 3 interactions.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Miscellaneous databases

EvolutionaryTraceiP31345.

Family and domain databases

InterProiIPR001591. RNA_pol_PB2_orthomyxovir.
[Graphical view]
PfamiPF00604. Flu_PB2. 1 hit.
[Graphical view]
ProDomiPD001667. RNA_pol_PB2_orthomyxovir. 1 hit.
[Graphical view] [Entries sharing at least one domain]
ProtoNetiSearch...

Entry informationi

Entry nameiPB2_I75A3
AccessioniPrimary (citable) accession number: P31345
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: December 9, 2015
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.