P31334 (RM09_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
54S ribosomal protein L9, mitochondrial

Alternative name(s):
YmL9

Gene names

Name:	MRPL9
Ordered Locus Names:	YGR220C
ORF Names:	G8520

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	269 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Subunit structure	Component of the mitochondrial large ribosomal subunit. Mature mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S subunit contains at least 33 different proteins and 1 molecule of RNA (15S). The 54S subunit contains at least 45 different proteins and 1 molecule of RNA (21S). Ref.6
Subcellular location	Mitochondrion Ref.7 Ref.9.
Miscellaneous	Present with 3200 molecules/cell in log phase SD medium. Ref.8
Sequence similarities	Belongs to the ribosomal protein L3P family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Molecular function	Ribonucleoprotein Ribosomal protein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	translation Inferred from electronic annotation. Source: InterPro
Cellular component	mitochondrion Inferred from direct assay. Source: SGD ribosome Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	structural constituent of ribosome Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 19	19	Mitochondrion Ref.1 Ref.6
	Chain	20 – 269	250	54S ribosomal protein L9, mitochondrial		PRO_0000030536
Experimental info
	Sequence conflict	21	1	S → V AA sequence Ref.6
	Sequence conflict	139	1	E → Q in CAA46148. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31334 [UniParc]. Last modified October 1, 1996. Version 3. Checksum: A09A4DA5CE9337E5	FASTA	269	29,790
	10 20 30 40 50 60 MSKFLQGSIF SISKLHVRYS STRPFLVAPS IANSITTEAP AINHSPELAN ARKWLPKRCG 70 80 90 100 110 120 LITRKKGMMP YFDKSTGERS AATILEVNNV EVIMHRTSEV NGYFACQVGY GSRHLSKVSR 130 140 150 160 170 180 QMLGHFASKV VNPKEHVAEF RVKDEKGLIP PGTLLKPSFF KEGQYVDVRS VSKGKGFTGV 190 200 210 220 230 240 MKRYGFKGLR ASHGTSIMHR HGGSYGQNQD PGRVLPGRKM PGHMGNEHVT IQNVKVLKVD 250 260 DENNVIWVKG SVAGPKNSFV KIQDAIKKT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"YmL9, a nucleus-encoded mitochondrial ribosomal protein of yeast, is homologous to L3 ribosomal proteins from all natural kingdoms and photosynthetic organelles." Graack H.-R., Grohmann L., Kitakawa M., Schaefer K.L., Kruft V. Eur. J. Biochem. 206:373-380(1992) [PubMed: 1597181] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 20-53; 176-182 AND 257-261. Strain: 07173.
[2]	"Sequence analysis of the 43 kb CRM1-YLM9-PET54-DIE2-SMI1-PHO81-YHB4-PFK1 region from the right arm of Saccharomyces cerevisiae chromosome VII." van der Aart Q.J.M., Kleine K., Steensma H.Y. Yeast 12:385-390(1996) [PubMed: 8701610] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[3]	"The nucleotide sequence of Saccharomyces cerevisiae chromosome VII." Tettelin H., Agostoni-Carbone M.L., Albermann K., Albers M., Arroyo J., Backes U., Barreiros T., Bertani I., Bjourson A.J., Brueckner M., Bruschi C.V., Carignani G., Castagnoli L., Cerdan E., Clemente M.L., Coblenz A., Coglievina M., Coissac E. , Defoor E., Del Bino S., Delius H., Delneri D., de Wergifosse P., Dujon B., Durand P., Entian K.-D., Eraso P., Escribano V., Fabiani L., Fartmann B., Feroli F., Feuermann M., Frontali L., Garcia-Gonzalez M., Garcia-Saez M.I., Goffeau A., Guerreiro P., Hani J., Hansen M., Hebling U., Hernandez K., Heumann K., Hilger F., Hofmann B., Indge K.J., James C.M., Klima R., Koetter P., Kramer B., Kramer W., Lauquin G., Leuther H., Louis E.J., Maillier E., Marconi A., Martegani E., Mazon M.J., Mazzoni C., McReynolds A.D.K., Melchioretto P., Mewes H.-W., Minenkova O., Mueller-Auer S., Nawrocki A., Netter P., Neu R., Nombela C., Oliver S.G., Panzeri L., Paoluzi S., Plevani P., Portetelle D., Portillo F., Potier S., Purnelle B., Rieger M., Riles L., Rinaldi T., Robben J., Rodrigues-Pousada C., Rodriguez-Belmonte E., Rodriguez-Torres A.M., Rose M., Ruzzi M., Saliola M., Sanchez-Perez M., Schaefer B., Schaefer M., Scharfe M., Schmidheini T., Schreer A., Skala J., Souciet J.-L., Steensma H.Y., Talla E., Thierry A., Vandenbol M., van der Aart Q.J.M., Van Dyck L., Vanoni M., Verhasselt P., Voet M., Volckaert G., Wambutt R., Watson M.D., Weber N., Wedler E., Wedler H., Wipfli P., Wolf K., Wright L.F., Zaccaria P., Zimmermann M., Zollner A., Kleine K. Nature 387:81-84(1997) [PubMed: 9169869] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 96604 / S288c / FY1679.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Mitochondrial ribosomes of yeast: isolation of individual proteins and N-terminal sequencing." Graack H.-R., Grohmann L., Choli T. FEBS Lett. 242:4-8(1988) [PubMed: 3060376] [Abstract] Cited for: PROTEIN SEQUENCE OF 20-53, SUBUNIT. Strain: 07173.
[7]	"Global analysis of protein localization in budding yeast." Huh W.-K., Falvo J.V., Gerke L.C., Carroll A.S., Howson R.W., Weissman J.S., O'Shea E.K. Nature 425:686-691(2003) [PubMed: 14562095] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
[8]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[9]	"The proteome of Saccharomyces cerevisiae mitochondria." Sickmann A., Reinders J., Wagner Y., Joppich C., Zahedi R.P., Meyer H.E., Schoenfisch B., Perschil I., Chacinska A., Guiard B., Rehling P., Pfanner N., Meisinger C. Proc. Natl. Acad. Sci. U.S.A. 100:13207-13212(2003) [PubMed: 14576278] [Abstract] Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY. Strain: ATCC 76625 / YPH499.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X65014 Genomic DNA. Translation: CAA46148.1. X87941 Genomic DNA. Translation: CAA61168.1. Z73004 Genomic DNA. Translation: CAA97248.1. AY558274 Genomic DNA. Translation: AAS56600.1. BK006941 Genomic DNA. Translation: DAA08313.1.
PIR	R5BYL3. S23455.
RefSeq	NP_011736.1. NM_001181349.1.
3D structure databases
ProteinModelPortal	P31334.
SMR	P31334. Positions 59-268.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-6806N.
IntAct	P31334. 46 interactions.
MINT	MINT-613241.
STRING	P31334.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YGR220C; YGR220C; YGR220C.
GeneID	853135.
KEGG	sce:YGR220C.
NMPDR	fig|4932.3.peg.2864.
Organism-specific databases
CYGD	YGR220c.
SGD	S000003452. MRPL9.
Phylogenomic databases
eggNOG	fuNOG08127.
GeneTree	EFGT00050000005833.
HOGENOM	HBG734653.
OMA	VGMTRLF.
OrthoDB	EOG4KM2CG.
Gene expression databases
ArrayExpress	P31334.
Genevestigator	P31334.
GermOnline	YGR220C. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR000597. Ribosomal_L3. IPR019927. Ribosomal_L3_bac/org-type. IPR019926. Ribosomal_L3_CS. IPR009000. Transl_elong_init/rib_B-barrel. [Graphical view]
PANTHER	PTHR11229. PTHR11229. 1 hit.
Pfam	PF00297. Ribosomal_L3. 1 hit. [Graphical view]
SUPFAM	SSF50447. Translat_factor. 1 hit.
TIGRFAMs	TIGR03625. L3_bact. 1 hit.
PROSITE	PS00474. RIBOSOMAL_L3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	973195.

Entry information

Entry name

RM09_YEAST

Accession

Primary (citable) accession number: P31334
Secondary accession number(s): D6VV02

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 107 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program