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Protein

54S ribosomal protein L9, mitochondrial

Gene

MRPL9

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Component of the mitochondrial ribosome (mitoribosome), a dedicated translation machinery responsible for the synthesis of mitochondrial genome-encoded proteins, including at least some of the essential transmembrane subunits of the mitochondrial respiratory chain. The mitoribosomes are attached to the mitochondrial inner membrane and translation products are cotranslationally integrated into the membrane.2 Publications

Miscellaneous

Present with 3200 molecules/cell in log phase SD medium.1 Publication

GO - Molecular functioni

  • structural constituent of ribosome Source: SGD

GO - Biological processi

  • mitochondrial translation Source: SGD

Keywordsi

Molecular functionRibonucleoprotein, Ribosomal protein

Enzyme and pathway databases

BioCyciYEAST:G3O-30901-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
54S ribosomal protein L9, mitochondrial
Alternative name(s):
Mitochondrial large ribosomal subunit protein uL3m1 Publication
YmL9
Gene namesi
Name:MRPL9
Ordered Locus Names:YGR220C
ORF Names:G8520
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VII

Organism-specific databases

EuPathDBiFungiDB:YGR220C.
SGDiS000003452. MRPL9.

Subcellular locationi

GO - Cellular componenti

  • mitochondrial large ribosomal subunit Source: SGD

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 19Mitochondrion2 PublicationsAdd BLAST19
ChainiPRO_000003053620 – 26954S ribosomal protein L9, mitochondrialAdd BLAST250

Proteomic databases

MaxQBiP31334.
PRIDEiP31334.

Interactioni

Subunit structurei

Component of the mitochondrial large ribosomal subunit (mt-LSU). Mature yeast 74S mitochondrial ribosomes consist of a small (37S) and a large (54S) subunit. The 37S small subunit contains a 15S ribosomal RNA (15S mt-rRNA) and 34 different proteins. The 54S large subunit contains a 21S rRNA (21S mt-rRNA) and 46 different proteins.2 Publications

Protein-protein interaction databases

BioGridi33473. 131 interactors.
DIPiDIP-6806N.
IntActiP31334. 46 interactors.
MINTiMINT-613241.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20C1-269[»]
5MRCelectron microscopy3.25C21-269[»]
5MREelectron microscopy3.75C21-269[»]
5MRFelectron microscopy4.97C21-269[»]
ProteinModelPortaliP31334.
SMRiP31334.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

GeneTreeiENSGT00390000011422.
HOGENOMiHOG000100368.
InParanoidiP31334.
KOiK02906.
OMAiGHMGQVR.
OrthoDBiEOG092C45IZ.

Family and domain databases

HAMAPiMF_01325_B. Ribosomal_L3_B. 1 hit.
InterProiView protein in InterPro
IPR000597. Ribosomal_L3.
IPR019927. Ribosomal_L3_bac/org-type.
IPR019926. Ribosomal_L3_CS.
IPR009000. Transl_B-barrel.
PANTHERiPTHR11229. PTHR11229. 1 hit.
PfamiView protein in Pfam
PF00297. Ribosomal_L3. 1 hit.
SUPFAMiSSF50447. SSF50447. 1 hit.
TIGRFAMsiTIGR03625. L3_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00474. RIBOSOMAL_L3. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31334-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKFLQGSIF SISKLHVRYS STRPFLVAPS IANSITTEAP AINHSPELAN
60 70 80 90 100
ARKWLPKRCG LITRKKGMMP YFDKSTGERS AATILEVNNV EVIMHRTSEV
110 120 130 140 150
NGYFACQVGY GSRHLSKVSR QMLGHFASKV VNPKEHVAEF RVKDEKGLIP
160 170 180 190 200
PGTLLKPSFF KEGQYVDVRS VSKGKGFTGV MKRYGFKGLR ASHGTSIMHR
210 220 230 240 250
HGGSYGQNQD PGRVLPGRKM PGHMGNEHVT IQNVKVLKVD DENNVIWVKG
260
SVAGPKNSFV KIQDAIKKT
Length:269
Mass (Da):29,790
Last modified:October 1, 1996 - v3
Checksum:iA09A4DA5CE9337E5
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti21S → V AA sequence (PubMed:3060376).Curated1
Sequence conflicti139E → Q in CAA46148 (PubMed:1597181).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65014 Genomic DNA. Translation: CAA46148.1.
X87941 Genomic DNA. Translation: CAA61168.1.
Z73004 Genomic DNA. Translation: CAA97248.1.
AY558274 Genomic DNA. Translation: AAS56600.1.
BK006941 Genomic DNA. Translation: DAA08313.1.
PIRiS23455. R5BYL3.
RefSeqiNP_011736.1. NM_001181349.1.

Genome annotation databases

EnsemblFungiiYGR220C; YGR220C; YGR220C.
GeneIDi853135.
KEGGisce:YGR220C.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X65014 Genomic DNA. Translation: CAA46148.1.
X87941 Genomic DNA. Translation: CAA61168.1.
Z73004 Genomic DNA. Translation: CAA97248.1.
AY558274 Genomic DNA. Translation: AAS56600.1.
BK006941 Genomic DNA. Translation: DAA08313.1.
PIRiS23455. R5BYL3.
RefSeqiNP_011736.1. NM_001181349.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3J6Belectron microscopy3.20C1-269[»]
5MRCelectron microscopy3.25C21-269[»]
5MREelectron microscopy3.75C21-269[»]
5MRFelectron microscopy4.97C21-269[»]
ProteinModelPortaliP31334.
SMRiP31334.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi33473. 131 interactors.
DIPiDIP-6806N.
IntActiP31334. 46 interactors.
MINTiMINT-613241.

Proteomic databases

MaxQBiP31334.
PRIDEiP31334.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiYGR220C; YGR220C; YGR220C.
GeneIDi853135.
KEGGisce:YGR220C.

Organism-specific databases

EuPathDBiFungiDB:YGR220C.
SGDiS000003452. MRPL9.

Phylogenomic databases

GeneTreeiENSGT00390000011422.
HOGENOMiHOG000100368.
InParanoidiP31334.
KOiK02906.
OMAiGHMGQVR.
OrthoDBiEOG092C45IZ.

Enzyme and pathway databases

BioCyciYEAST:G3O-30901-MONOMER.

Miscellaneous databases

PROiPR:P31334.

Family and domain databases

HAMAPiMF_01325_B. Ribosomal_L3_B. 1 hit.
InterProiView protein in InterPro
IPR000597. Ribosomal_L3.
IPR019927. Ribosomal_L3_bac/org-type.
IPR019926. Ribosomal_L3_CS.
IPR009000. Transl_B-barrel.
PANTHERiPTHR11229. PTHR11229. 1 hit.
PfamiView protein in Pfam
PF00297. Ribosomal_L3. 1 hit.
SUPFAMiSSF50447. SSF50447. 1 hit.
TIGRFAMsiTIGR03625. L3_bact. 1 hit.
PROSITEiView protein in PROSITE
PS00474. RIBOSOMAL_L3. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiRM09_YEAST
AccessioniPrimary (citable) accession number: P31334
Secondary accession number(s): D6VV02
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 1, 1996
Last modified: April 12, 2017
This is version 144 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. Ribosomal proteins
    Ribosomal proteins families and list of entries
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome VII
    Yeast (Saccharomyces cerevisiae) chromosome VII: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.