P31332 (L_SYNV) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
RNA-directed RNA polymerase L
Short name=Protein L

Alternative name(s):
Large structural protein
Replicase
Transcriptase

Including the following 3 domains:

1. RNA-directed RNA polymerase
   EC=2.7.7.48
2. mRNA (guanine-N(7)-)-methyltransferase
   EC=2.1.1.56
3. mRNA guanylyltransferase
   EC=2.7.7.-

Gene names

Name:

L

Organism

Sonchus yellow net virus (SYNV) [Complete proteome]

Taxonomic identifier

11307 [NCBI]

Taxonomic lineage

Viruses › ssRNA negative-strand viruses › Mononegavirales › Rhabdoviridae › Nucleorhabdovirus

Virus host

Lactuca sativa (Garden lettuce) [TaxID: 4236]
Bidens pilosa (Hairy beggarticks) (Cobbler's pegs) [TaxID: 42337]
Sonchus oleraceus (Common sowthistle) [TaxID: 50207]
Aphis [TaxID: 80764]

Protein attributes

Sequence length	2116 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Displays RNA-directed RNA polymerase, mRNA guanylyl transferase, mRNA (guanine-N(7)-)-methyltransferase and poly(A) synthetase activities. The viral mRNA guanylyl transferase displays a different biochemical reaction than the cellular enzyme. The template is composed of the viral RNA tightly encapsidated by the nucleoprotein (N). Functions either as transcriptase or as replicase. The transcriptase synthesizes subsequently five subgenomic RNAs, assuring their capping and polyadenylation by a stuttering mechanism. The replicase mode is dependent on intracellular N protein concentration. In this mode, the polymerase replicates the whole viral genome without recognizing the transcriptional signals By similarity.
Catalytic activity	Nucleoside triphosphate + RNA(n) = diphosphate + RNA(n+1). S-adenosyl-L-methionine + G(5')pppR-RNA = S-adenosyl-L-homocysteine + m7G(5')pppR-RNA.
Subunit structure	Interacts with the P protein By similarity.
Subcellular location	Virion Potential. Host cytoplasm By similarity.
Sequence similarities	Belongs to the rhabdoviridae protein L family. Contains 1 RdRp catalytic domain.

Ontologies

Keywords
Biological process	RNA replication mRNA capping mRNA processing
Cellular component	Host cytoplasm Virion
Ligand	ATP-binding Nucleotide-binding S-adenosyl-L-methionine
Molecular function	Methyltransferase Nucleotidyltransferase RNA-directed RNA polymerase Transferase
Technical term	Complete proteome Multifunctional enzyme
Gene Ontology (GO)
Biological process	RNA biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW mRNA capping Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro host cell cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell virion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW RNA-directed RNA polymerase activity Inferred from electronic annotation. Source: UniProtKB-KW mRNA (guanine-N7-)-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 2116	2116	RNA-directed RNA polymerase L		PRO_0000222842
Regions
	Domain	634 – 818	185	RdRp catalytic

Sequences

	Sequence		Length	Mass (Da)
	P31332 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 74D188F3D4CF1D80	FASTA	2,116	241,538
	10 20 30 40 50 60 MEGMDHWENA KYFQGIEDIE EDTRQPTVDS MSSGTYHCKS ALRSHKDNMK LFLYRRDFLI 70 80 90 100 110 120 FSHRFNGLPY DEQYLGVLPK LWSCFYDKTH DLSGFLDQYA SREHCTPSDS FSRWADPTVL 130 140 150 160 170 180 HLYDDPIIRN LLASENKVLN FLEGGISDIL DKYQICIKRN IRLIYLHLFL NLALIVLNHT 190 200 210 220 230 240 DADSMPDRRV ELNGVTFKLE EGVILCEYNE YLKIYVLKGA VIWDMPAYRQ VLQKDLFLTI 250 260 270 280 290 300 CDKISERINI VIGATIITAL SHKTNLDDPD SHLYDACINM IKIGDNILVN HGNRGFDLLG 310 320 330 340 350 360 KFEAYCVACI LTYDDQRIWN PLEFLNNLIE DDRINQPDLY NDANNLVAFL RKQPIVILAE 370 380 390 400 410 420 LHGLWRIWGH PIIDLEGGMK KMEATCTKQS PVSVEETRVC ERTMKLTFFT NYYDKHHHYP 430 440 450 460 470 480 LSTLTHPDHF NLYSQYLSER DKIEYLANKD IAFEHSYIMR CIRRNKKIFQ RSSLYNHKDW 490 500 510 520 530 540 DQVVILQSFQ IPKSVNLATM IKDKAISMTR SELIESVNTK NSVFDSTKRR GILKWLNEQS 550 560 570 580 590 600 DKIYNFLMRI DDKGLDEDDC IIGLYPKERE MKTKARFFSL MSYKLRMYVT STEELLGKYV 610 620 630 640 650 660 LKYFPMITMS DNLLSMVIRL FDMTTLIGDK GVAVTYSMNI DFSKWNQNMR ERTNAGIFDN 670 680 690 700 710 720 LDRILGFRSL ISRTHSIFKA CYLYLCSGEY VPVISNNQLT AQSPWSRTGD ESGKEGLRQK 730 740 750 760 770 780 GWTITTVCDI LSLAFKYNAR IQLIGGGDNQ VLTVTMLPSE SMQSQGRDSQ LLKVRERMTS 790 800 810 820 830 840 FRNALAKKMV KRGLPLKLEE TWISHNLLMY NKIMYYSGVP LRGRLKVISR LFSNSNVGVT 850 860 870 880 890 900 SLGGITSTLG TGFQSISTKD YTPTLAWLIS RVFTDIYIST YHLLNPISGT QRLDKQVLMS 910 920 930 940 950 960 RGNIRQGRNE LGGETSVPII NKIRNHAALA TDHTLDLDSL LICVLYYHKI LGGPGIGPPT 970 980 990 1000 1010 1020 AYVMKGFPDP LSEGLTFNYL VITNVLNERT KRKIISVTKV MKNRNQHWEH LLEDPVSVNH 1030 1040 1050 1060 1070 1080 DAPPHGIAAL RAQAEAVMRS AKITNIGFKN LIDIGDNQYL RDLSEKLCSP NDLEPRLLHD 1090 1100 1110 1120 1130 1140 IVGSTIPGFV NSVLSRVDQS TTINKIAGNS DVVTSIYLSE MSYYLYLSKK VNTQDGHAIG 1150 1160 1170 1180 1190 1200 SCPTRDSKML RNWTWGKNII GVTTPHPLGY LKRERHSESS SCDNNYIRVL TKRIGNSWEL 1210 1220 1230 1240 1250 1260 RRGQFRPYFG SYTEEKFKMT TLASAYGDES ILKRAIKIQK LLGWRYHQGS SLYNLIQKIL 1270 1280 1290 1300 1310 1320 TCVTDADPNK FLPLPDEITG DVEHRYHDMA TKHGGIPSNL IHLYTHASCN TSTFINHSKG 1330 1340 1350 1360 1370 1380 AANESLHFQA AIIWTCMQSI CRTSASSSVS DISHYHEACN QCIVKLEDPI ESDYSTSDIS 1390 1400 1410 1420 1430 1440 LMSCPANDLM YVKEDDIPVH FHTTMEFYRA SSSSTVLKKI KKIEDAEVIS SRMTWVVTLC 1450 1460 1470 1480 1490 1500 SHLLNQDTIK HSTWKLISED LSKEEVMFIV MSITMYIMSE QDIPVHSASL SDFRTLYEKN 1510 1520 1530 1540 1550 1560 KDIIDRVLGI EALNDAVSGV SFYNNRCSDD QCLRWKETSD QILSHYKTTG TCAVKYQAPH 1570 1580 1590 1600 1610 1620 FRICTRLVYL MTNPSCQSCP CCLGVIKDDS NDGPIMCQLH GELAGPCGYH LCSLDKLNKT 1630 1640 1650 1660 1670 1680 KKGLNMSKVF YTDGTISAQH DAKNRPTKKR PHGENSLTEM FKRAKTSREN RILKQNKESY 1690 1700 1710 1720 1730 1740 LFIQPRLMVD LFIDMGSMWE KTQISDQGSH IIPAHTNPIV LSKKSDLYVP AAISKFVSNG 1750 1760 1770 1780 1790 1800 FLIMDAVERA LGKPSKPITE HSQLSVNISY GIEYHPEIKR ETVQLLRFVN ELAYTGYGGG 1810 1820 1830 1840 1850 1860 VTICITLFPI FISDIEAVDP RLISDIIYRY RADSSDYACI RLTDMGDMCF DINNILSDCD 1870 1880 1890 1900 1910 1920 ACLSYDPGCW SQDANLVYII SDTSDIMIKA KEFETLHSFN KFYSVECLAP RFFMSSSTVS 1930 1940 1950 1960 1970 1980 ALVISKSSSI NSIDYDRLAE IHLDRRGTQM WDLSRLFANM TMRDGLTEMI KCIYNNVSGE 1990 2000 2010 2020 2030 2040 ATILTSQSLV DAAISKIKVD ILRGLIDMVR GERMNWRTQY MIQILLGVMI LTSDNPEDYR 2050 2060 2070 2080 2090 2100 REISKYNNAV VLLQKPRRIK LIRDHLGGAD RKFYHATTNN GLLGSSLNDV THILEGILYI 2110 THRTSRKLCT SISLEV

References

[1]	"Structure of the L (polymerase) protein gene of sonchus yellow net virus." Choi T.-J., Kuwata S., Koonin E.V., Heaton L.A., Jackson A.O. Virology 189:31-39(1992) [PubMed: 1604816] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: ATCC PV-263.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L32603 mRNA. Translation: AAA50385.1. M87829 mRNA. Translation: AAA47896.1.
PIR	ZLVNSY. A40230.
RefSeq	NP_042286.1. NC_001615.2.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	1489882.
Family and domain databases
InterPro	IPR014023. RNA-dir_pol_cat. [Graphical view]
Pfam	PF00946. Paramyx_RNA_pol. 1 hit. [Graphical view]
PROSITE	PS50526. RDRP_SSRNA_NEG_NONSEG. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

L_SYNV

Accession

Primary (citable) accession number: P31332

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	December 14, 2011
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program