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Protein

U2-agatoxin-Aop1a

Gene
N/A
Organism
Allagelena opulenta (Funnel weaving spider) (Agelena opulenta)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Insect-selective toxin causing rapid but reversible paralysis in crickets. Suppresses the excitatory postsynaptic potentials evoked in lobster neuromuscular synaptic preparations, possibly by blocking the presynaptic calcium channel (Cav). Induces instantaneous reversible paralysis when injected into crickets.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei9Critical for insecticidal activityBy similarity1
Sitei28Critical for insecticidal activityBy similarity1
Sitei33Critical for insecticidal activityBy similarity1

Keywordsi

Molecular functionCalcium channel impairing toxin, Ion channel impairing toxin, Neurotoxin, Presynaptic neurotoxin, Toxin, Voltage-gated calcium channel impairing toxin

Names & Taxonomyi

Protein namesi
Recommended name:
U2-agatoxin-Aop1a
Short name:
U2-AGTX-Aop1a
Alternative name(s):
Agelenin
OrganismiAllagelena opulenta (Funnel weaving spider) (Agelena opulenta)
Taxonomic identifieri29934 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAraneaeAraneomorphaeEntelegynaeAgelenidaeAllagelena

Organism-specific databases

ArachnoServeriAS000286. U2-agatoxin-Aop1a.

Subcellular locationi

GO - Cellular componenti

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Toxic dosei

PD50 is 291 pmol/g of crickets.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
PeptideiPRO_00000449851 – 35U2-agatoxin-Aop1aAdd BLAST35

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi3 ↔ 19
Disulfide bondi10 ↔ 24
Disulfide bondi18 ↔ 34
Modified residuei35Leucine amide3 Publications1

Keywords - PTMi

Amidation, Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Structurei

Secondary structure

135
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi12 – 15Combined sources4
Beta strandi24 – 28Combined sources5
Beta strandi31 – 34Combined sources4

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E2SNMR-A1-35[»]
ProteinModelPortaliP31328.
SMRiP31328.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31328.

Family & Domainsi

Domaini

The presence of a 'disulfide through disulfide knot' structurally defines this protein as a knottin.

Keywords - Domaini

Knottin

Sequencei

Sequence statusi: Complete.

P31328-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30 
GGCLPHNRFC NALSGPRCCS GLKCKELSIW DSRCL
Length:35
Mass (Da):3,825
Last modified:July 1, 1993 - v1
Checksum:iCBE6462825350D90
GO

Sequence databases

PIRiA60959.

Cross-referencesi

Sequence databases

PIRiA60959.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2E2SNMR-A1-35[»]
ProteinModelPortaliP31328.
SMRiP31328.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

ArachnoServeriAS000286. U2-agatoxin-Aop1a.

Miscellaneous databases

EvolutionaryTraceiP31328.

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiTXAG_ALLOP
AccessioniPrimary (citable) accession number: P31328
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 69 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Miscellaneous

The primary structure of the mature peptide is identical to that of U2-agatoxin-Ao1a (Agelenin) from Agelena orientalis (AC Q5Y4Y5).Curated
This toxin does not inhibit mammalian Cav2.1/CACNA1A, Cav2.2/CACNA1B and Cav2.3/CACNA1E calcium channels.1 Publication

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.