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P31325

- PHKG2_RAT

UniProt

P31325 - PHKG2_RAT

Protein

Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

Gene

Phkg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM.1 Publication

    Catalytic activityi

    2 ATP + phosphorylase b = 2 ADP + phosphorylase a.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei53 – 531ATPPROSITE-ProRule annotation
    Active sitei153 – 1531Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi30 – 389ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: RGD
    2. calmodulin binding Source: RGD
    3. enzyme binding Source: RGD
    4. phosphorylase kinase activity Source: RGD

    GO - Biological processi

    1. glycogen biosynthetic process Source: InterPro
    2. glycogen metabolic process Source: RGD
    3. protein phosphorylation Source: RGD

    Keywords - Molecular functioni

    Kinase, Serine/threonine-protein kinase, Transferase

    Keywords - Biological processi

    Carbohydrate metabolism, Glycogen metabolism

    Keywords - Ligandi

    ATP-binding, Calmodulin-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.11.19. 5301.
    ReactomeiREACT_227999. Glycogen breakdown (glycogenolysis).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (EC:2.7.11.19)
    Short name:
    PHK-gamma-LT
    Short name:
    PHK-gamma-T
    Alternative name(s):
    Phosphorylase kinase subunit gamma-2
    Gene namesi
    Name:Phkg2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 1

    Organism-specific databases

    RGDi620024. Phkg2.

    Subcellular locationi

    GO - Cellular componenti

    1. phosphorylase kinase complex Source: RGD

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 406406Phosphorylase b kinase gamma catalytic chain, liver/testis isoformPRO_0000086514Add
    BLAST

    Proteomic databases

    PaxDbiP31325.
    PRIDEiP31325.

    Expressioni

    Gene expression databases

    GenevestigatoriP31325.

    Interactioni

    Subunit structurei

    Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

    Structurei

    3D structure databases

    ProteinModelPortaliP31325.
    SMRiP31325. Positions 20-293.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini24 – 291268Protein kinasePROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni306 – 33025Calmodulin-binding (domain-N)By similarityAdd
    BLAST
    Regioni346 – 37025Calmodulin-binding (domain-C)By similarityAdd
    BLAST

    Sequence similaritiesi

    Contains 1 protein kinase domain.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG0515.
    GeneTreeiENSGT00750000117716.
    HOGENOMiHOG000233016.
    HOVERGENiHBG106193.
    InParanoidiP31325.
    KOiK00871.
    OMAiHRPPGPF.
    OrthoDBiEOG7JMGDM.
    PhylomeDBiP31325.
    TreeFamiTF320349.

    Family and domain databases

    InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view]
    PANTHERiPTHR24347. PTHR24347. 1 hit.
    PfamiPF00069. Pkinase. 1 hit.
    [Graphical view]
    PRINTSiPR01049. PHOSPHBKNASE.
    SMARTiSM00220. S_TKc. 1 hit.
    [Graphical view]
    SUPFAMiSSF56112. SSF56112. 1 hit.
    PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31325-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTLDVGPEDE LPDWAAAKEF YQKYDPKDII GRGVSSVVRR CVHRATGDEF    50
    AVKIMEVSAE RLSLEQLEEV RDATRREMHI LRQVAGHPHI ITLIDSYESS 100
    SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VNFLHVNNIV 150
    HRDLKPENIL LDDNMQIRLS DFGFSCHLEP GEKLRELCGT PGYLAPEILK 200
    CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY 250
    QFSSPEWDDR SNTVKDLIAK LLQVDPNARL TAEQALQHPF FERCKGSQPW 300
    NLTPRQRFRV AVWTILAAGR VALSSHRLRP LTKNALLRDP YALRPVRRLI 350
    DNCAFRLYGH WVKKGEQQNR AALFQHQPPR PFPIIATDLE GDSSAITEDE 400
    VTLVRS 406
    Length:406
    Mass (Da):46,678
    Last modified:July 1, 1993 - v1
    Checksum:i7D5DB86B8E8D5BDA
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73808 mRNA. Translation: AAA41863.1.
    BC128715 mRNA. Translation: AAI28716.1.
    PIRiA42034.
    RefSeqiNP_542151.1. NM_080584.4.
    XP_003749039.1. XM_003748991.2.
    XP_006230264.1. XM_006230202.1.
    UniGeneiRn.11153.

    Genome annotation databases

    EnsembliENSRNOT00000025419; ENSRNOP00000025419; ENSRNOG00000018725.
    ENSRNOT00000071284; ENSRNOP00000067209; ENSRNOG00000050421.
    GeneIDi100909429.
    140671.
    KEGGirno:100909429.
    rno:140671.
    UCSCiRGD:620024. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M73808 mRNA. Translation: AAA41863.1 .
    BC128715 mRNA. Translation: AAI28716.1 .
    PIRi A42034.
    RefSeqi NP_542151.1. NM_080584.4.
    XP_003749039.1. XM_003748991.2.
    XP_006230264.1. XM_006230202.1.
    UniGenei Rn.11153.

    3D structure databases

    ProteinModelPortali P31325.
    SMRi P31325. Positions 20-293.
    ModBasei Search...
    MobiDBi Search...

    Proteomic databases

    PaxDbi P31325.
    PRIDEi P31325.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000025419 ; ENSRNOP00000025419 ; ENSRNOG00000018725 .
    ENSRNOT00000071284 ; ENSRNOP00000067209 ; ENSRNOG00000050421 .
    GeneIDi 100909429.
    140671.
    KEGGi rno:100909429.
    rno:140671.
    UCSCi RGD:620024. rat.

    Organism-specific databases

    CTDi 5261.
    RGDi 620024. Phkg2.

    Phylogenomic databases

    eggNOGi COG0515.
    GeneTreei ENSGT00750000117716.
    HOGENOMi HOG000233016.
    HOVERGENi HBG106193.
    InParanoidi P31325.
    KOi K00871.
    OMAi HRPPGPF.
    OrthoDBi EOG7JMGDM.
    PhylomeDBi P31325.
    TreeFami TF320349.

    Enzyme and pathway databases

    BRENDAi 2.7.11.19. 5301.
    Reactomei REACT_227999. Glycogen breakdown (glycogenolysis).

    Miscellaneous databases

    NextBioi 620659.
    PROi P31325.

    Gene expression databases

    Genevestigatori P31325.

    Family and domain databases

    InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
    IPR011009. Kinase-like_dom.
    IPR002291. Phosph_kin_gamma.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR002290. Ser/Thr_dual-sp_kinase_dom.
    IPR008271. Ser/Thr_kinase_AS.
    [Graphical view ]
    PANTHERi PTHR24347. PTHR24347. 1 hit.
    Pfami PF00069. Pkinase. 1 hit.
    [Graphical view ]
    PRINTSi PR01049. PHOSPHBKNASE.
    SMARTi SM00220. S_TKc. 1 hit.
    [Graphical view ]
    SUPFAMi SSF56112. SSF56112. 1 hit.
    PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 1 hit.
    PS00108. PROTEIN_KINASE_ST. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning and enzymatic analysis of the rat homolog of 'PhK-gamma T,' an isoform of phosphorylase kinase catalytic subunit."
      Calalb M.B., Fox D.T., Hanks S.K.
      J. Biol. Chem. 267:1455-1463(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN PHOSPHORYLATION OF PYGM.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Testis.

    Entry informationi

    Entry nameiPHKG2_RAT
    AccessioniPrimary (citable) accession number: P31325
    Secondary accession number(s): A1A5L8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 117 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3