Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

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P31325 (PHKG2_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 107. History...

Clusters with 100%, 90%, 50% identity |

Documents (1) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform
Short name=PHK-gamma-LT
Short name=PHK-gamma-T
EC=2.7.11.19

Alternative name(s):
Phosphorylase kinase subunit gamma-2

Gene names

Name:

Phkg2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	406 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM. Ref.1
Catalytic activity	2 ATP + phosphorylase b = 2 ADP + phosphorylase a.
Subunit structure	Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.
Sequence similarities	Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. Contains 1 protein kinase domain.

Ontologies

Keywords
Biological process	Carbohydrate metabolism Glycogen metabolism
Ligand	ATP-binding Calmodulin-binding Nucleotide-binding
Molecular function	Kinase Serine/threonine-protein kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	glycogen biosynthetic process Inferred from electronic annotation. Source: InterPro glycogen metabolic process Inferred from direct assay Ref.1 PubMed 6281247. Source: RGD
Cellular_component	phosphorylase kinase complex Inferred from direct assay PubMed 6809757. Source: RGD
Molecular_function	ATP binding Inferred from direct assay PubMed 6281247 PubMed 6809757. Source: RGD calmodulin binding Inferred from direct assay Ref.1. Source: RGD enzyme binding Inferred from direct assay Ref.1 PubMed 6281247 PubMed 6809757. Source: RGD phosphorylase kinase activity Inferred from direct assay Ref.1 PubMed 6281247 PubMed 6809757. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 406

406

Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

PRO_0000086514

Regions

Domain

24 – 291

268

Protein kinase

Nucleotide binding

30 – 38

ATP By similarity

Region

306 – 330

Calmodulin-binding (domain-N) By similarity

Region

346 – 370

Calmodulin-binding (domain-C) By similarity

Sites

Active site

153

Proton acceptor By similarity

Binding site

ATP By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P31325 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 7D5DB86B8E8D5BDA
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FASTA

406

46,678

        10         20         30         40         50         60 
MTLDVGPEDE LPDWAAAKEF YQKYDPKDII GRGVSSVVRR CVHRATGDEF AVKIMEVSAE 

        70         80         90        100        110        120 
RLSLEQLEEV RDATRREMHI LRQVAGHPHI ITLIDSYESS SFMFLVFDLM RKGELFDYLT 

       130        140        150        160        170        180 
EKVALSEKET RSIMRSLLEA VNFLHVNNIV HRDLKPENIL LDDNMQIRLS DFGFSCHLEP 

       190        200        210        220        230        240 
GEKLRELCGT PGYLAPEILK CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL 

       250        260        270        280        290        300 
MLRMIMEGQY QFSSPEWDDR SNTVKDLIAK LLQVDPNARL TAEQALQHPF FERCKGSQPW 

       310        320        330        340        350        360 
NLTPRQRFRV AVWTILAAGR VALSSHRLRP LTKNALLRDP YALRPVRRLI DNCAFRLYGH 

       370        380        390        400 
WVKKGEQQNR AALFQHQPPR PFPIIATDLE GDSSAITEDE VTLVRS

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning and enzymatic analysis of the rat homolog of 'PhK-gamma T,' an isoform of phosphorylase kinase catalytic subunit." Calalb M.B., Fox D.T., Hanks S.K. J. Biol. Chem. 267:1455-1463(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN PHOSPHORYLATION OF PYGM.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M73808 mRNA. Translation: AAA41863.1. BC128715 mRNA. Translation: AAI28716.1.
IPI	IPI00211673.
PIR	A42034.
RefSeq	NP_542151.1. NM_080584.4. XP_003749039.1. XM_003748991.1. XP_003749040.1. XM_003748992.1.
UniGene	Rn.11153.
3D structure databases
ProteinModelPortal	P31325.
SMR	P31325. Positions 20-293.
ModBase	Search...
Proteomic databases
PaxDb	P31325.
PRIDE	P31325.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000025419; ENSRNOP00000025419; ENSRNOG00000018725. ENSRNOT00000071284; ENSRNOP00000067209; ENSRNOG00000050421.
GeneID	100909429. 140671.
KEGG	rno:100909429. rno:140671.
UCSC	RGD:620024. rat.
Organism-specific databases
CTD	5261.
RGD	620024. Phkg2.
Phylogenomic databases
eggNOG	COG0515.
GeneTree	ENSGT00680000100008.
HOGENOM	HOG000233016.
HOVERGEN	HBG106193.
InParanoid	P31325.
KO	K00871.
OMA	HQPPGPF.
OrthoDB	EOG4CNQR5.
Enzyme and pathway databases
BRENDA	2.7.11.19. 5301.
Gene expression databases
Genevestigator	P31325.
GermOnline	ENSRNOG00000018725. Rattus norvegicus.
Family and domain databases
InterPro	IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase. IPR011009. Kinase-like_dom. IPR002291. Phosph_kin_gamma. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR002290. Ser/Thr_dual-sp_kinase_dom. IPR008271. Ser/Thr_kinase_AS. [Graphical view]
PANTHER	PTHR24347. PTHR24347. 1 hit.
Pfam	PF00069. Pkinase. 1 hit. [Graphical view]
PRINTS	PR01049. PHOSPHBKNASE.
SMART	SM00220. S_TKc. 1 hit. [Graphical view]
SUPFAM	SSF56112. Kinase_like. 1 hit.
PROSITE	PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 1 hit. PS00108. PROTEIN_KINASE_ST. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	620659.

Entry information

Entry name

PHKG2_RAT

Accession

Primary (citable) accession number: P31325
Secondary accession number(s): A1A5L8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents