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P31325

- PHKG2_RAT

UniProt

P31325 - PHKG2_RAT

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Protein

Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

Gene
Phkg2
Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM.1 Publication

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei53 – 531ATP By similarity
Active sitei153 – 1531Proton acceptor By similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi30 – 389ATP By similarity

GO - Molecular functioni

  1. ATP binding Source: RGD
  2. calmodulin binding Source: RGD
  3. enzyme binding Source: RGD
  4. phosphorylase kinase activity Source: RGD

GO - Biological processi

  1. glycogen biosynthetic process Source: InterPro
  2. glycogen metabolic process Source: RGD
  3. protein phosphorylation Source: RGD
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Serine/threonine-protein kinase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen metabolism

Keywords - Ligandi

ATP-binding, Calmodulin-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.19. 5301.
ReactomeiREACT_227999. Glycogen breakdown (glycogenolysis).

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (EC:2.7.11.19)
Short name:
PHK-gamma-LT
Short name:
PHK-gamma-T
Alternative name(s):
Phosphorylase kinase subunit gamma-2
Gene namesi
Name:Phkg2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494: Chromosome 1

Organism-specific databases

RGDi620024. Phkg2.

Subcellular locationi

GO - Cellular componenti

  1. phosphorylase kinase complex Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 406406Phosphorylase b kinase gamma catalytic chain, liver/testis isoformPRO_0000086514Add
BLAST

Proteomic databases

PaxDbiP31325.
PRIDEiP31325.

Expressioni

Gene expression databases

GenevestigatoriP31325.

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

Structurei

3D structure databases

ProteinModelPortaliP31325.
SMRiP31325. Positions 20-293.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini24 – 291268Protein kinaseAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni306 – 33025Calmodulin-binding (domain-N) By similarityAdd
BLAST
Regioni346 – 37025Calmodulin-binding (domain-C) By similarityAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0515.
GeneTreeiENSGT00750000117716.
HOGENOMiHOG000233016.
HOVERGENiHBG106193.
InParanoidiP31325.
KOiK00871.
OMAiHRPPGPF.
OrthoDBiEOG7JMGDM.
PhylomeDBiP31325.
TreeFamiTF320349.

Family and domain databases

InterProiIPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view]
PANTHERiPTHR24347. PTHR24347. 1 hit.
PfamiPF00069. Pkinase. 1 hit.
[Graphical view]
PRINTSiPR01049. PHOSPHBKNASE.
SMARTiSM00220. S_TKc. 1 hit.
[Graphical view]
SUPFAMiSSF56112. SSF56112. 1 hit.
PROSITEiPS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31325-1 [UniParc]FASTAAdd to Basket

« Hide

MTLDVGPEDE LPDWAAAKEF YQKYDPKDII GRGVSSVVRR CVHRATGDEF    50
AVKIMEVSAE RLSLEQLEEV RDATRREMHI LRQVAGHPHI ITLIDSYESS 100
SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VNFLHVNNIV 150
HRDLKPENIL LDDNMQIRLS DFGFSCHLEP GEKLRELCGT PGYLAPEILK 200
CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY 250
QFSSPEWDDR SNTVKDLIAK LLQVDPNARL TAEQALQHPF FERCKGSQPW 300
NLTPRQRFRV AVWTILAAGR VALSSHRLRP LTKNALLRDP YALRPVRRLI 350
DNCAFRLYGH WVKKGEQQNR AALFQHQPPR PFPIIATDLE GDSSAITEDE 400
VTLVRS 406
Length:406
Mass (Da):46,678
Last modified:July 1, 1993 - v1
Checksum:i7D5DB86B8E8D5BDA
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73808 mRNA. Translation: AAA41863.1.
BC128715 mRNA. Translation: AAI28716.1.
PIRiA42034.
RefSeqiNP_542151.1. NM_080584.4.
XP_003749039.1. XM_003748991.2.
XP_006230264.1. XM_006230202.1.
UniGeneiRn.11153.

Genome annotation databases

EnsembliENSRNOT00000025419; ENSRNOP00000025419; ENSRNOG00000018725.
ENSRNOT00000071284; ENSRNOP00000067209; ENSRNOG00000050421.
GeneIDi100909429.
140671.
KEGGirno:100909429.
rno:140671.
UCSCiRGD:620024. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M73808 mRNA. Translation: AAA41863.1 .
BC128715 mRNA. Translation: AAI28716.1 .
PIRi A42034.
RefSeqi NP_542151.1. NM_080584.4.
XP_003749039.1. XM_003748991.2.
XP_006230264.1. XM_006230202.1.
UniGenei Rn.11153.

3D structure databases

ProteinModelPortali P31325.
SMRi P31325. Positions 20-293.
ModBasei Search...
MobiDBi Search...

Proteomic databases

PaxDbi P31325.
PRIDEi P31325.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSRNOT00000025419 ; ENSRNOP00000025419 ; ENSRNOG00000018725 .
ENSRNOT00000071284 ; ENSRNOP00000067209 ; ENSRNOG00000050421 .
GeneIDi 100909429.
140671.
KEGGi rno:100909429.
rno:140671.
UCSCi RGD:620024. rat.

Organism-specific databases

CTDi 5261.
RGDi 620024. Phkg2.

Phylogenomic databases

eggNOGi COG0515.
GeneTreei ENSGT00750000117716.
HOGENOMi HOG000233016.
HOVERGENi HBG106193.
InParanoidi P31325.
KOi K00871.
OMAi HRPPGPF.
OrthoDBi EOG7JMGDM.
PhylomeDBi P31325.
TreeFami TF320349.

Enzyme and pathway databases

BRENDAi 2.7.11.19. 5301.
Reactomei REACT_227999. Glycogen breakdown (glycogenolysis).

Miscellaneous databases

NextBioi 620659.
PROi P31325.

Gene expression databases

Genevestigatori P31325.

Family and domain databases

InterProi IPR020636. Ca/CaM-dep_Ca-dep_prot_Kinase.
IPR011009. Kinase-like_dom.
IPR002291. Phosph_kin_gamma.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR002290. Ser/Thr_dual-sp_kinase_dom.
IPR008271. Ser/Thr_kinase_AS.
[Graphical view ]
PANTHERi PTHR24347. PTHR24347. 1 hit.
Pfami PF00069. Pkinase. 1 hit.
[Graphical view ]
PRINTSi PR01049. PHOSPHBKNASE.
SMARTi SM00220. S_TKc. 1 hit.
[Graphical view ]
SUPFAMi SSF56112. SSF56112. 1 hit.
PROSITEi PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 1 hit.
PS00108. PROTEIN_KINASE_ST. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning and enzymatic analysis of the rat homolog of 'PhK-gamma T,' an isoform of phosphorylase kinase catalytic subunit."
    Calalb M.B., Fox D.T., Hanks S.K.
    J. Biol. Chem. 267:1455-1463(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION IN PHOSPHORYLATION OF PYGM.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Testis.

Entry informationi

Entry nameiPHKG2_RAT
AccessioniPrimary (citable) accession number: P31325
Secondary accession number(s): A1A5L8
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 3, 2014
This is version 116 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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