Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphorylase b kinase gamma catalytic chain, liver/testis isoform

Gene

Phkg2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic subunit of the phosphorylase b kinase (PHK), which mediates the neural and hormonal regulation of glycogen breakdown (glycogenolysis) by phosphorylating and thereby activating glycogen phosphorylase. May regulate glycogeneolysis in the testis. In vitro, phosphorylates PYGM.1 Publication

Catalytic activityi

2 ATP + phosphorylase b = 2 ADP + phosphorylase a.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei53ATPPROSITE-ProRule annotation1
Active sitei153Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi30 – 38ATPPROSITE-ProRule annotation9

GO - Molecular functioni

  • ATP binding Source: RGD
  • calmodulin binding Source: RGD
  • enzyme binding Source: RGD
  • phosphorylase kinase activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionCalmodulin-binding, Kinase, Serine/threonine-protein kinase, Transferase
Biological processCarbohydrate metabolism, Glycogen metabolism
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.11.19 5301
ReactomeiR-RNO-70221 Glycogen breakdown (glycogenolysis)

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphorylase b kinase gamma catalytic chain, liver/testis isoform (EC:2.7.11.19)
Short name:
PHK-gamma-LT
Short name:
PHK-gamma-T
Alternative name(s):
Phosphorylase kinase subunit gamma-2
Gene namesi
Name:Phkg2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi

Organism-specific databases

RGDi620024 Phkg2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000865141 – 406Phosphorylase b kinase gamma catalytic chain, liver/testis isoformAdd BLAST406

Proteomic databases

PaxDbiP31325
PRIDEiP31325

Expressioni

Gene expression databases

BgeeiENSRNOG00000018725
GenevisibleiP31325 RN

Interactioni

Subunit structurei

Hexadecamer of 4 heterotetramers, each composed of alpha, beta, gamma, and delta subunits. Alpha (PHKA1 or PHKA2) and beta (PHKB) are regulatory subunits, gamma (PHKG1 or PHKG2) is the catalytic subunit, and delta is calmodulin.

GO - Molecular functioni

  • calmodulin binding Source: RGD
  • enzyme binding Source: RGD

Protein-protein interaction databases

BioGridi250831, 1 interactor
STRINGi10116.ENSRNOP00000067209

Structurei

3D structure databases

ProteinModelPortaliP31325
SMRiP31325
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini24 – 291Protein kinasePROSITE-ProRule annotationAdd BLAST268

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni306 – 330Calmodulin-binding (domain-N)By similarityAdd BLAST25
Regioni346 – 370Calmodulin-binding (domain-C)By similarityAdd BLAST25

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG0599 Eukaryota
ENOG410Y7VX LUCA
GeneTreeiENSGT00840000129744
HOGENOMiHOG000233016
HOVERGENiHBG106193
InParanoidiP31325
KOiK00871
OMAiWHRRQIL
OrthoDBiEOG091G07XZ
PhylomeDBiP31325
TreeFamiTF320349

Family and domain databases

InterProiView protein in InterPro
IPR011009 Kinase-like_dom_sf
IPR002291 Phosph_kin_gamma
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR008271 Ser/Thr_kinase_AS
PfamiView protein in Pfam
PF00069 Pkinase, 1 hit
PRINTSiPR01049 PHOSPHBKNASE
SMARTiView protein in SMART
SM00220 S_TKc, 1 hit
SUPFAMiSSF56112 SSF56112, 1 hit
PROSITEiView protein in PROSITE
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 1 hit
PS00108 PROTEIN_KINASE_ST, 1 hit

Sequencei

Sequence statusi: Complete.

P31325-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLDVGPEDE LPDWAAAKEF YQKYDPKDII GRGVSSVVRR CVHRATGDEF
60 70 80 90 100
AVKIMEVSAE RLSLEQLEEV RDATRREMHI LRQVAGHPHI ITLIDSYESS
110 120 130 140 150
SFMFLVFDLM RKGELFDYLT EKVALSEKET RSIMRSLLEA VNFLHVNNIV
160 170 180 190 200
HRDLKPENIL LDDNMQIRLS DFGFSCHLEP GEKLRELCGT PGYLAPEILK
210 220 230 240 250
CSMDETHPGY GKEVDLWACG VILFTLLAGS PPFWHRRQIL MLRMIMEGQY
260 270 280 290 300
QFSSPEWDDR SNTVKDLIAK LLQVDPNARL TAEQALQHPF FERCKGSQPW
310 320 330 340 350
NLTPRQRFRV AVWTILAAGR VALSSHRLRP LTKNALLRDP YALRPVRRLI
360 370 380 390 400
DNCAFRLYGH WVKKGEQQNR AALFQHQPPR PFPIIATDLE GDSSAITEDE

VTLVRS
Length:406
Mass (Da):46,678
Last modified:July 1, 1993 - v1
Checksum:i7D5DB86B8E8D5BDA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M73808 mRNA Translation: AAA41863.1
BC128715 mRNA Translation: AAI28716.1
PIRiA42034
RefSeqiNP_542151.1, NM_080584.4
XP_008758077.1, XM_008759855.2
UniGeneiRn.11153

Genome annotation databases

EnsembliENSRNOT00000025419; ENSRNOP00000025419; ENSRNOG00000018725
ENSRNOT00000087142; ENSRNOP00000072734; ENSRNOG00000018725
GeneIDi140671
KEGGirno:140671
UCSCiRGD:620024 rat

Entry informationi

Entry nameiPHKG2_RAT
AccessioniPrimary (citable) accession number: P31325
Secondary accession number(s): A1A5L8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 139 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Cookie policy

We would like to use anonymized google analytics cookies to gather statistics on how uniprot.org is used in aggregate. Learn more

UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health