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Protein

cAMP-dependent protein kinase type II-beta regulatory subunit

Gene

Prkar2b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei221cAMP 1By similarity1
Binding sitei230cAMP 1By similarity1
Binding sitei350cAMP 2By similarity1
Binding sitei359cAMP 2By similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 273cAMP 1Add BLAST122
Nucleotide bindingi274 – 416cAMP 2Add BLAST143

GO - Molecular functioni

GO - Biological processi

  • fatty acid metabolic process Source: MGI
  • learning Source: MGI
  • negative regulation of cAMP-dependent protein kinase activity Source: MGI
  • response to clozapine Source: Ensembl

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-163615 PKA activation
R-MMU-164378 PKA activation in glucagon signalling
R-MMU-180024 DARPP-32 events
R-MMU-2565942 Regulation of PLK1 Activity at G2/M Transition
R-MMU-380259 Loss of Nlp from mitotic centrosomes
R-MMU-380270 Recruitment of mitotic centrosome proteins and complexes
R-MMU-380320 Recruitment of NuMA to mitotic centrosomes
R-MMU-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-MMU-5610787 Hedgehog 'off' state
R-MMU-5620912 Anchoring of the basal body to the plasma membrane
R-MMU-8854518 AURKA Activation by TPX2
R-MMU-983231 Factors involved in megakaryocyte development and platelet production

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type II-beta regulatory subunit
Gene namesi
Name:Prkar2b
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:97760 Prkar2b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002053911 – 416cAMP-dependent protein kinase type II-beta regulatory subunitAdd BLAST416

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei83PhosphoserineCombined sources1
Modified residuei85PhosphoserineBy similarity1
Modified residuei112PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the activated catalytic chain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP31324
MaxQBiP31324
PaxDbiP31324
PeptideAtlasiP31324
PRIDEiP31324

PTM databases

iPTMnetiP31324
PhosphoSitePlusiP31324
SwissPalmiP31324

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiENSMUSG00000002997
ExpressionAtlasiP31324 baseline and differential
GenevisibleiP31324 MM

Interactioni

Subunit structurei

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with the phosphorylated form of PJA2 (By similarity). Forms a complex composed of PRKAR2B, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity (By similarity).By similarity

Binary interactionsi

Show more details

GO - Molecular functioni

Protein-protein interaction databases

BioGridi20236915 interactors.
CORUMiP31324
DIPiDIP-31571N
IntActiP31324 19 interactors.
MINTiP31324
STRINGi10090.ENSMUSP00000003079

Structurei

Secondary structure

1416
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi137 – 147Combined sources11
Helixi151 – 154Combined sources4
Helixi158 – 167Combined sources10
Beta strandi169 – 173Combined sources5
Beta strandi178 – 180Combined sources3
Beta strandi188 – 194Combined sources7
Beta strandi196 – 202Combined sources7
Beta strandi207 – 215Combined sources9
Helixi221 – 224Combined sources4
Beta strandi231 – 246Combined sources16
Helixi247 – 261Combined sources15
Beta strandi262 – 268Combined sources7
Helixi269 – 271Combined sources3
Helixi273 – 275Combined sources3
Helixi280 – 289Combined sources10
Beta strandi291 – 295Combined sources5
Beta strandi300 – 302Combined sources3
Beta strandi310 – 322Combined sources13
Beta strandi338 – 342Combined sources5
Beta strandi347 – 349Combined sources3
Helixi350 – 353Combined sources4
Beta strandi360 – 375Combined sources16
Helixi376 – 383Combined sources8
Helixi386 – 390Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3TNPX-ray2.30B/E1-416[»]
3TNQX-ray3.10A1-416[»]
4WBBX-ray2.80A1-416[»]
4X6QX-ray2.52B1-416[»]
ProteinModelPortaliP31324
SMRiP31324
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 151Dimerization and phosphorylationAdd BLAST151

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196668
HOVERGENiHBG002025
InParanoidiP31324
KOiK04739
OMAiILMESTF
OrthoDBiEOG091G0F1K
PhylomeDBiP31324
TreeFamiTF314920

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.102 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits

Sequencei

Sequence statusi: Complete.

P31324-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEIPAGLT ELLQGFTVEV LRHQPADLLE FALQHFTRLQ QENERKGAAR
60 70 80 90 100
FGHEGRTWGD AGAAAGGGIP SKGVNFAEEP MRSDSENGEE EEAAEAGAFN
110 120 130 140 150
APVINRFTRR ASVCAEAYNP DEEEDDAESR IIHPKTDDQR NRLQEACKDI
160 170 180 190 200
LLFKNLDPEQ MSQVLDAMFE KLVKEGEHVI DQGDDGDNFY VIDRGTFDIY
210 220 230 240 250
VKCDGVGRCV GNYDNRGSFG ELALMYNTPR AATITATSPG ALWGLDRVTF
260 270 280 290 300
RRIIVKNNAK KRKMYESFIE SLPFLKSLEV SERLKVVDVI GTKVYNDGEQ
310 320 330 340 350
IIAQGDLADS FFIVESGEVK ITMKRKGKSE VEENGAVEIA RCFRGQYFGE
360 370 380 390 400
LALVTNKPRA ASAHAIGTVK CLAMDVQAFE RLLGPCMEIM KRNIATYEEQ
410
LVALFGTNMD IVEPTA
Length:416
Mass (Da):46,167
Last modified:January 23, 2007 - v3
Checksum:i24196C3037789827
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti69I → T in AAH48710 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK041013 mRNA Translation: BAC30779.1
AK138963 mRNA Translation: BAE23838.1
CT010463 Genomic DNA Translation: CAM17140.1
BC048710 mRNA Translation: AAH48710.1
M68861 Genomic DNA Translation: AAA40057.1
CCDSiCCDS25869.1
PIRiPQ0161
RefSeqiNP_035288.2, NM_011158.3
XP_006515077.1, XM_006515014.3
UniGeneiMm.25594

Genome annotation databases

EnsembliENSMUST00000003079; ENSMUSP00000003079; ENSMUSG00000002997
ENSMUST00000036497; ENSMUSP00000039797; ENSMUSG00000002997
GeneIDi19088
KEGGimmu:19088
UCSCiuc007nhx.2 mouse

Similar proteinsi

Entry informationi

Entry nameiKAP3_MOUSE
AccessioniPrimary (citable) accession number: P31324
Secondary accession number(s): B1B199
, Q3UTZ1, Q80ZM4, Q8BRZ7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: January 23, 2007
Last modified: March 28, 2018
This is version 164 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome