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Protein

cAMP-dependent protein kinase type II-beta regulatory subunit

Gene

PRKAR2B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei223cAMP 11
Binding sitei232cAMP 11
Binding sitei352cAMP 21
Binding sitei361cAMP 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi154 – 275cAMP 1Add BLAST122
Nucleotide bindingi276 – 418cAMP 2Add BLAST143

GO - Molecular functioni

  • cAMP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase inhibitor activity Source: BHF-UCL
  • cAMP-dependent protein kinase regulator activity Source: BHF-UCL
  • protein domain specific binding Source: Ensembl
  • protein kinase A catalytic subunit binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-163615 PKA activation
R-HSA-164378 PKA activation in glucagon signalling
R-HSA-180024 DARPP-32 events
R-HSA-2565942 Regulation of PLK1 Activity at G2/M Transition
R-HSA-380259 Loss of Nlp from mitotic centrosomes
R-HSA-380270 Recruitment of mitotic centrosome proteins and complexes
R-HSA-380284 Loss of proteins required for interphase microtubule organization from the centrosome
R-HSA-380320 Recruitment of NuMA to mitotic centrosomes
R-HSA-381676 Glucagon-like Peptide-1 (GLP1) regulates insulin secretion
R-HSA-432040 Vasopressin regulates renal water homeostasis via Aquaporins
R-HSA-5610787 Hedgehog 'off' state
R-HSA-5620912 Anchoring of the basal body to the plasma membrane
R-HSA-8854518 AURKA Activation by TPX2
R-HSA-983231 Factors involved in megakaryocyte development and platelet production
SIGNORiP31323

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type II-beta regulatory subunit
Gene namesi
Name:PRKAR2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

EuPathDBiHostDB:ENSG00000005249.12
HGNCiHGNC:9392 PRKAR2B
MIMi176912 gene
neXtProtiNX_P31323

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5577
OpenTargetsiENSG00000005249
PharmGKBiPA33758

Chemistry databases

DrugBankiDB02527 Cyclic Adenosine Monophosphate
GuidetoPHARMACOLOGYi1475

Polymorphism and mutation databases

BioMutaiPRKAR2B
DMDMi206729918

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002053901 – 418cAMP-dependent protein kinase type II-beta regulatory subunitAdd BLAST418

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei69PhosphothreonineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei85PhosphoserineCombined sources1
Modified residuei114PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylated by the activated catalytic chain.

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiP31323
MaxQBiP31323
PaxDbiP31323
PeptideAtlasiP31323
PRIDEiP31323

2D gel databases

OGPiP31323

PTM databases

iPTMnetiP31323
PhosphoSitePlusiP31323

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiENSG00000005249
CleanExiHS_PRKAR2B
ExpressionAtlasiP31323 baseline and differential
GenevisibleiP31323 HS

Organism-specific databases

HPAiHPA008421

Interactioni

Subunit structurei

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with the phosphorylated form of PJA2. Forms a complex composed of PRKAR2B, GSK3B and GSKIP through GSKIP interaction; facilitates PKA-induced phosphorylation and regulates GSK3B activity (PubMed:25920809).2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein domain specific binding Source: Ensembl
  • protein kinase A catalytic subunit binding Source: BHF-UCL
  • ubiquitin protein ligase binding Source: UniProtKB

Protein-protein interaction databases

BioGridi111563, 68 interactors
CORUMiP31323
DIPiDIP-554N
IntActiP31323, 38 interactors
MINTiP31323
STRINGi9606.ENSP00000265717

Chemistry databases

BindingDBiP31323

Structurei

3D structure databases

ProteinModelPortaliP31323
SMRiP31323
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 153Dimerization and phosphorylationAdd BLAST152

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113 Eukaryota
COG0664 LUCA
GeneTreeiENSGT00530000062947
HOGENOMiHOG000196668
HOVERGENiHBG002025
InParanoidiP31323
KOiK04739
OMAiILMESTF
OrthoDBiEOG091G0F1K
PhylomeDBiP31323
TreeFamiTF314920

Family and domain databases

CDDicd00038 CAP_ED, 2 hits
Gene3Di2.60.120.10, 2 hits
InterProiView protein in InterPro
IPR012198 cAMP_dep_PK_reg_su
IPR003117 cAMP_dep_PK_reg_su_I/II_a/b
IPR018490 cNMP-bd-like
IPR018488 cNMP-bd_CS
IPR000595 cNMP-bd_dom
IPR014710 RmlC-like_jellyroll
PfamiView protein in Pfam
PF00027 cNMP_binding, 2 hits
PF02197 RIIa, 1 hit
PIRSFiPIRSF000548 PK_regulatory, 1 hit
SMARTiView protein in SMART
SM00100 cNMP, 2 hits
SM00394 RIIa, 1 hit
SUPFAMiSSF51206 SSF51206, 2 hits
PROSITEiView protein in PROSITE
PS00888 CNMP_BINDING_1, 2 hits
PS00889 CNMP_BINDING_2, 2 hits
PS50042 CNMP_BINDING_3, 2 hits

Sequencei

Sequence statusi: Complete.

P31323-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSIEIPAGLT ELLQGFTVEV LRHQPADLLE FALQHFTRLQ QENERKGTAR
60 70 80 90 100
FGHEGRTWGD LGAAAGGGTP SKGVNFAEEP MQSDSEDGEE EEAAPADAGA
110 120 130 140 150
FNAPVINRFT RRASVCAEAY NPDEEEDDAE SRIIHPKTDD QRNRLQEACK
160 170 180 190 200
DILLFKNLDP EQMSQVLDAM FEKLVKDGEH VIDQGDDGDN FYVIDRGTFD
210 220 230 240 250
IYVKCDGVGR CVGNYDNRGS FGELALMYNT PRAATITATS PGALWGLDRV
260 270 280 290 300
TFRRIIVKNN AKKRKMYESF IESLPFLKSL EFSERLKVVD VIGTKVYNDG
310 320 330 340 350
EQIIAQGDSA DSFFIVESGE VKITMKRKGK SEVEENGAVE IARCSRGQYF
360 370 380 390 400
GELALVTNKP RAASAHAIGT VKCLAMDVQA FERLLGPCME IMKRNIATYE
410
EQLVALFGTN MDIVEPTA
Length:418
Mass (Da):46,302
Last modified:September 23, 2008 - v3
Checksum:i83F402CCFFEFD186
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti341 – 342IA → MP in AAA60099 (PubMed:2851102).Curated2

Natural variant

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Natural variantiVAR_046549335E → D. Corresponds to variant dbSNP:rs3729881Ensembl.1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M31158 mRNA Translation: AAA60099.1
AK291441 mRNA Translation: BAF84130.1
CH236947 Genomic DNA Translation: EAL24395.1
CH471070 Genomic DNA Translation: EAW83390.1
CCDSiCCDS5740.1
PIRiA40915 OKHUR2
RefSeqiNP_002727.2, NM_002736.2
UniGeneiHs.433068

Genome annotation databases

EnsembliENST00000265717; ENSP00000265717; ENSG00000005249
ENST00000639737; ENSP00000491687; ENSG00000284096
GeneIDi5577
KEGGihsa:5577
UCSCiuc003vdx.4 human

Keywords - Coding sequence diversityi

Polymorphism

Similar proteinsi

Entry informationi

Entry nameiKAP3_HUMAN
AccessioniPrimary (citable) accession number: P31323
Secondary accession number(s): A4D0R9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 23, 2008
Last modified: May 23, 2018
This is version 175 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

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