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P31323

- KAP3_HUMAN

UniProt

P31323 - KAP3_HUMAN

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Protein
cAMP-dependent protein kinase type II-beta regulatory subunit
Gene
PRKAR2B
Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells. Type II regulatory chains mediate membrane association by binding to anchoring proteins, including the MAP2 kinase.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei223 – 2231cAMP 1
Binding sitei232 – 2321cAMP 1
Binding sitei352 – 3521cAMP 2
Binding sitei361 – 3611cAMP 2

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi154 – 275122cAMP 1
Add
BLAST
Nucleotide bindingi276 – 418143cAMP 2
Add
BLAST

GO - Molecular functioni

  1. cAMP binding Source: UniProtKB-KW
  2. cAMP-dependent protein kinase inhibitor activity Source: BHF-UCL
  3. cAMP-dependent protein kinase regulator activity Source: BHF-UCL
  4. protein kinase A catalytic subunit binding Source: BHF-UCL
  5. ubiquitin protein ligase binding Source: UniProtKB

GO - Biological processi

  1. G2/M transition of mitotic cell cycle Source: Reactome
  2. activation of phospholipase C activity Source: Reactome
  3. activation of protein kinase A activity Source: Reactome
  4. blood coagulation Source: Reactome
  5. cellular response to glucagon stimulus Source: Reactome
  6. energy reserve metabolic process Source: Reactome
  7. epidermal growth factor receptor signaling pathway Source: Reactome
  8. fatty acid metabolic process Source: Ensembl
  9. fibroblast growth factor receptor signaling pathway Source: Reactome
  10. innate immune response Source: Reactome
  11. intracellular signal transduction Source: ProtInc
  12. learning Source: Ensembl
  13. mitotic cell cycle Source: Reactome
  14. negative regulation of cAMP-dependent protein kinase activity Source: BHF-UCL
  15. neurotrophin TRK receptor signaling pathway Source: Reactome
  16. regulation of insulin secretion Source: Reactome
  17. signal transduction Source: Reactome
  18. small molecule metabolic process Source: Reactome
  19. transmembrane transport Source: Reactome
  20. water transport Source: Reactome
Complete GO annotation...

Keywords - Ligandi

cAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_15296. Recruitment of mitotic centrosome proteins and complexes.
REACT_15334. DARPP-32 events.
REACT_15364. Loss of Nlp from mitotic centrosomes.
REACT_15451. Loss of proteins required for interphase microtubule organization from the centrosome.
REACT_15530. PKA activation.
REACT_160315. Regulation of PLK1 Activity at G2/M Transition.
REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_1946. PKA activation in glucagon signalling.
REACT_24023. Regulation of water balance by renal Aquaporins.
REACT_24970. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type II-beta regulatory subunit
Gene namesi
Name:PRKAR2B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
ProteomesiUP000005640: Chromosome 7

Organism-specific databases

HGNCiHGNC:9392. PRKAR2B.

Subcellular locationi

Cytoplasm. Cell membrane
Note: Colocalizes with PJA2 in the cytoplasm and at the cell membrane.1 Publication

GO - Cellular componenti

  1. cAMP-dependent protein kinase complex Source: InterPro
  2. centrosome Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. cytosol Source: Reactome
  5. plasma membrane Source: UniProtKB
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Cytoplasm, Membrane

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA33758.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed Inferred
Chaini2 – 418417cAMP-dependent protein kinase type II-beta regulatory subunit
PRO_0000205390Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine Inferred
Modified residuei83 – 831Phosphoserine1 Publication
Modified residuei85 – 851Phosphoserine1 Publication
Modified residuei114 – 1141Phosphoserine3 Publications

Post-translational modificationi

Phosphorylated by the activated catalytic chain.

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP31323.
PaxDbiP31323.
PRIDEiP31323.

2D gel databases

OGPiP31323.

PTM databases

PhosphoSiteiP31323.

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

ArrayExpressiP31323.
BgeeiP31323.
CleanExiHS_PRKAR2B.
GenevestigatoriP31323.

Organism-specific databases

HPAiHPA008421.

Interactioni

Subunit structurei

The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. Interacts with the phosphorylated form of PJA2.1 Publication

Protein-protein interaction databases

BioGridi111563. 12 interactions.
DIPiDIP-554N.
IntActiP31323. 6 interactions.
MINTiMINT-2782562.
STRINGi9606.ENSP00000265717.

Structurei

3D structure databases

ProteinModelPortaliP31323.
SMRiP31323. Positions 8-410.

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni2 – 153152Dimerization and phosphorylation
Add
BLAST

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiCOG0664.
HOGENOMiHOG000196668.
HOVERGENiHBG002025.
InParanoidiP31323.
KOiK04739.
OMAiPMHSDSE.
OrthoDBiEOG76T9RR.
PhylomeDBiP31323.
TreeFamiTF314920.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiIPR002373. cAMP/cGMP_kin.
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view]
PfamiPF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view]
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
PRINTSiPR00103. CAMPKINASE.
SMARTiSM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view]
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiPS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31323-1 [UniParc]FASTAAdd to Basket

« Hide

MSIEIPAGLT ELLQGFTVEV LRHQPADLLE FALQHFTRLQ QENERKGTAR    50
FGHEGRTWGD LGAAAGGGTP SKGVNFAEEP MQSDSEDGEE EEAAPADAGA 100
FNAPVINRFT RRASVCAEAY NPDEEEDDAE SRIIHPKTDD QRNRLQEACK 150
DILLFKNLDP EQMSQVLDAM FEKLVKDGEH VIDQGDDGDN FYVIDRGTFD 200
IYVKCDGVGR CVGNYDNRGS FGELALMYNT PRAATITATS PGALWGLDRV 250
TFRRIIVKNN AKKRKMYESF IESLPFLKSL EFSERLKVVD VIGTKVYNDG 300
EQIIAQGDSA DSFFIVESGE VKITMKRKGK SEVEENGAVE IARCSRGQYF 350
GELALVTNKP RAASAHAIGT VKCLAMDVQA FERLLGPCME IMKRNIATYE 400
EQLVALFGTN MDIVEPTA 418
Length:418
Mass (Da):46,302
Last modified:September 23, 2008 - v3
Checksum:i83F402CCFFEFD186
GO

Natural variant

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Natural varianti335 – 3351E → D.
Corresponds to variant rs3729881 [ dbSNP | Ensembl ].
VAR_046549

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti341 – 3422IA → MP in AAA60099. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31158 mRNA. Translation: AAA60099.1.
AK291441 mRNA. Translation: BAF84130.1.
CH236947 Genomic DNA. Translation: EAL24395.1.
CH471070 Genomic DNA. Translation: EAW83390.1.
CCDSiCCDS5740.1.
PIRiA40915. OKHUR2.
RefSeqiNP_002727.2. NM_002736.2.
UniGeneiHs.433068.

Genome annotation databases

EnsembliENST00000265717; ENSP00000265717; ENSG00000005249.
GeneIDi5577.
KEGGihsa:5577.
UCSCiuc003vdx.3. human.

Polymorphism databases

DMDMi206729918.

Keywords - Coding sequence diversityi

Polymorphism

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M31158 mRNA. Translation: AAA60099.1 .
AK291441 mRNA. Translation: BAF84130.1 .
CH236947 Genomic DNA. Translation: EAL24395.1 .
CH471070 Genomic DNA. Translation: EAW83390.1 .
CCDSi CCDS5740.1.
PIRi A40915. OKHUR2.
RefSeqi NP_002727.2. NM_002736.2.
UniGenei Hs.433068.

3D structure databases

ProteinModelPortali P31323.
SMRi P31323. Positions 8-410.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 111563. 12 interactions.
DIPi DIP-554N.
IntActi P31323. 6 interactions.
MINTi MINT-2782562.
STRINGi 9606.ENSP00000265717.

Chemistry

BindingDBi P31323.

PTM databases

PhosphoSitei P31323.

Polymorphism databases

DMDMi 206729918.

2D gel databases

OGPi P31323.

Proteomic databases

MaxQBi P31323.
PaxDbi P31323.
PRIDEi P31323.

Protocols and materials databases

DNASUi 5577.
Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENST00000265717 ; ENSP00000265717 ; ENSG00000005249 .
GeneIDi 5577.
KEGGi hsa:5577.
UCSCi uc003vdx.3. human.

Organism-specific databases

CTDi 5577.
GeneCardsi GC07P106685.
H-InvDB HIX0025309.
HGNCi HGNC:9392. PRKAR2B.
HPAi HPA008421.
MIMi 176912. gene.
neXtProti NX_P31323.
PharmGKBi PA33758.
GenAtlasi Search...

Phylogenomic databases

eggNOGi COG0664.
HOGENOMi HOG000196668.
HOVERGENi HBG002025.
InParanoidi P31323.
KOi K04739.
OMAi PMHSDSE.
OrthoDBi EOG76T9RR.
PhylomeDBi P31323.
TreeFami TF314920.

Enzyme and pathway databases

Reactomei REACT_15296. Recruitment of mitotic centrosome proteins and complexes.
REACT_15334. DARPP-32 events.
REACT_15364. Loss of Nlp from mitotic centrosomes.
REACT_15451. Loss of proteins required for interphase microtubule organization from the centrosome.
REACT_15530. PKA activation.
REACT_160315. Regulation of PLK1 Activity at G2/M Transition.
REACT_18274. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
REACT_1946. PKA activation in glucagon signalling.
REACT_24023. Regulation of water balance by renal Aquaporins.
REACT_24970. Factors involved in megakaryocyte development and platelet production.

Miscellaneous databases

GeneWikii PRKAR2B.
GenomeRNAii 5577.
NextBioi 21624.
PROi P31323.
SOURCEi Search...

Gene expression databases

ArrayExpressi P31323.
Bgeei P31323.
CleanExi HS_PRKAR2B.
Genevestigatori P31323.

Family and domain databases

Gene3Di 2.60.120.10. 2 hits.
InterProi IPR002373. cAMP/cGMP_kin.
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
[Graphical view ]
Pfami PF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
[Graphical view ]
PIRSFi PIRSF000548. PK_regulatory. 1 hit.
PRINTSi PR00103. CAMPKINASE.
SMARTi SM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
[Graphical view ]
SUPFAMi SSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEi PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular cloning, complementary deoxyribonucleic acid structure and predicted full-length amino acid sequence of the hormone-inducible regulatory subunit of 3'-5'-cyclic adenosine monophosphate-dependent protein kinase from human testis."
    Levy F.O., Oeyen O., Sandberg M., Tasken K., Eskild W., Hansson V., Jahnsen T.
    Mol. Endocrinol. 2:1364-1373(1988) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Testis.
  2. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Brain.
  3. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  5. "Large-scale phosphoproteome analysis of human liver tissue by enrichment and fractionation of phosphopeptides with strong anion exchange chromatography."
    Han G., Ye M., Zhou H., Jiang X., Feng S., Jiang X., Tian R., Wan D., Zou H., Gu J.
    Proteomics 8:1346-1361(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Liver.
  6. "Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach."
    Gauci S., Helbig A.O., Slijper M., Krijgsveld J., Heck A.J., Mohammed S.
    Anal. Chem. 81:4493-4501(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  7. "Quantitative phosphoproteomic analysis of T cell receptor signaling reveals system-wide modulation of protein-protein interactions."
    Mayya V., Lundgren D.H., Hwang S.-I., Rezaul K., Wu L., Eng J.K., Rodionov V., Han D.K.
    Sci. Signal. 2:RA46-RA46(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Leukemic T-cell.
  8. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  9. Cited for: SUBCELLULAR LOCATION, INTERACTION WITH PJA2.
  10. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-83; SER-85 AND SER-114, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKAP3_HUMAN
AccessioniPrimary (citable) accession number: P31323
Secondary accession number(s): A4D0R9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 23, 2008
Last modified: September 3, 2014
This is version 138 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. Human entries with polymorphisms or disease mutations
    List of human entries with polymorphisms or disease mutations
  3. Human polymorphisms and disease mutations
    Index of human polymorphisms and disease mutations
  4. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  5. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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