Reviewed,
UniProtKB/Swiss-Prot P31321 (KAP1_HUMAN)
Last modified
November 25, 2008.
Version 80.
History...
Clusters with 100%,
90%,
50% identity |
Documents (3) |
Third-party data |
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Names and origin
| Protein names | Recommended name: cAMP-dependent protein kinase type I-beta regulatory subunit | ||
| Gene names |
| ||
| Organism | Homo sapiens (Human) | ||
| Taxonomic identifier | 9606 [NCBI] | ||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo |
Protein attributes
| Sequence length | 381 AA. |
| Sequence status | Complete. |
| Sequence processing | The displayed sequence is further processed into a mature form. |
| Protein existence | Evidence at protein level. |
General annotation (Comments)
| Subunit structure | The inactive form of the enzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP produces two active catalytic monomers and a regulatory dimer that binds four cAMP molecules. |
| Tissue specificity | Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible. |
| Post-translational modification | The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity. |
| Sequence similarities | Belongs to the cAMP-dependent kinase regulatory chain family. Contains 2 cyclic nucleotide-binding domains. |
Ontologies
Keywords | |
|---|---|
| Domain | Repeat |
| Ligand | Nucleotide-binding cAMP cAMP-binding |
| PTM | Acetylation Nitration Phosphoprotein |
Gene Ontology (GO) | |
| Biological process | hormone-mediated signaling Inferred from Experiment. Source: Reactome regulation of protein amino acid phosphorylationInferred from electronic annotation. Source: InterPro |
| Cellular component | cAMP-dependent protein kinase complex Ref.1 Non-traceable author statement. Source: UniProtKB |
| Molecular function | cAMP binding Inferred from electronic annotation. Source: UniProtKB-KW cAMP-dependent protein kinase regulator activity Ref.1Non-traceable author statement. Source: UniProtKB |
| Complete GO annotation... | |
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Initiator methionine | 1 | 1 | Removed By similarity | ||||||
| Chain | 2 – 381 | 380 | cAMP-dependent protein kinase type I-beta regulatory subunit | PRO_0000205381 | |||||
Regions | |||||||||
| Nucleotide binding | 137 – 254 | 118 | cAMP 1 | ||||||
| Nucleotide binding | 255 – 381 | 127 | cAMP 2 | ||||||
| Region | 2 – 136 | 135 | Dimerization and phosphorylation | ||||||
| Motif | 96 – 100 | 5 | Pseudophosphorylation motif | ||||||
Sites | |||||||||
| Binding site | 202 | 1 | cAMP 1 | ||||||
| Binding site | 211 | 1 | cAMP 1 | ||||||
| Binding site | 326 | 1 | cAMP 2 | ||||||
| Binding site | 335 | 1 | cAMP 2 | ||||||
Amino acid modifications | |||||||||
| Modified residue | 2 | 1 | N-acetylalanine Probable | ||||||
| Modified residue | 21 | 1 | Nitrated tyrosine | ||||||
| Modified residue | 77 | 1 | Phosphoserine | ||||||
| Modified residue | 83 | 1 | Phosphoserine | ||||||
| Disulfide bond | 18 | Interchain (with C-39) By similarity | |||||||
| Disulfide bond | 39 | Interchain (with C-18) By similarity | |||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Molecular cloning, cDNA structure and tissue-specific expression of the human regulatory subunit RI beta of cAMP-dependent protein kinases." Solberg R., Tasken K., Keiserud A., Jahnsen T. Biochem. Biophys. Res. Commun. 176:166-172(1991) [PubMed: 1708242] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. |
| [2] | "Human regulatory subunit RI beta of cAMP-dependent protein kinases: expression, holoenzyme formation and microinjection into living cells." Solberg R., Tasken K., Wen W., Coghlan V.M., Meinkoth J.L., Scott J.D., Jahnsen T., Taylor S.S. Exp. Cell Res. 214:595-605(1994) [PubMed: 7925653] [Abstract] Cited for: SEQUENCE REVISION TO 98-100. Tissue: Testis. |
| [3] | "Nitroproteins from a human pituitary adenoma tissue discovered with a nitrotyrosine affinity column and tandem mass spectrometry." Zhan X., Desiderio D.M. Anal. Biochem. 354:279-289(2006) [PubMed: 16777052] [Abstract] Cited for: NITRATION [LARGE SCALE ANALYSIS] AT TYR-21, MASS SPECTROMETRY. Tissue: Pituitary adenoma. |
| [4] | "A quantitative atlas of mitotic phosphorylation." Dephoure N., Zhou C., Villen J., Beausoleil S.A., Bakalarski C.E., Elledge S.J., Gygi S.P. Proc. Natl. Acad. Sci. U.S.A. 105:10762-10767(2008) [PubMed: 18669648] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-77 AND SER-83, MASS SPECTROMETRY. |
Cross-references
Sequence databases | |
|---|---|
| M65066 mRNA. Translation: AAC37564.1. | |
| PIR | OKHUR1. JH0392. |
| UniGene | Hs.520851 |
3D structure databases | |
| HSSP | HSSP built from PDB template 1RGS based on UniProtKB P00514. |
| SMR | P31321. Positions 14-63, 112-378. |
| ModBase | Search... |
PTM databases | |
| PhosphoSite | P31321. |
Genome annotation databases | |
| Ensembl | ENSG00000188191. Homo sapiens. [Contig view] |
Organism-specific databases | |
| H-InvDB | HIX0017594. |
| HGNC | HGNC:9390. PRKAR1B. |
| MIM | 176911. gene. |
| PharmGKB | PA33756. |
| GenAtlas | Search... |
| GeneCards | Search... |
Phylogenomic databases | |
| HOGENOM | P31321. |
| HOVERGEN | P31321. |
Enzyme and pathway databases | |
| Reactome | REACT_1505. Integration of energy metabolism. |
Gene expression databases | |
| ArrayExpress | P31321. |
| CleanEx | HS_PRKAR1B. |
| GermOnline | ENSG00000188191. Homo sapiens. |
Family and domain databases | |
| InterPro | IPR003117. cAMP-dep_prot_kin_reg_I/II_a/b. IPR002373. cAMP/cGMP_kin. IPR000595. cNMP_bd. IPR012198. PK_regulatory. IPR014710. RmlC-like_jellyroll. [Graphical view] |
| Gene3D | G3DSA:2.60.120.10. RmlC-like_jellyroll. 2 hits. |
| Pfam | PF00027. cNMP_binding. 2 hits. PF02197. RIIa. 1 hit. [Graphical view] |
| PIRSF | PIRSF000548. PK_regulatory. 1 hit. |
| PRINTS | PR00103. CAMPKINASE. |
| SMART | SM00100. cNMP. 2 hits. SM00394. RIIa. 1 hit. [Graphical view] |
| PROSITE | PS00888. CNMP_BINDING_1. 2 hits. PS00889. CNMP_BINDING_2. 2 hits. PS50042. CNMP_BINDING_3. 2 hits. [Graphical view] |
| ProtoNet | Search... |
Other Resources | |
| LinkHub | P31321. |
| SOURCE | Search... |
Entry information
| Entry name | KAP1_HUMAN | ||||||||
| Accession | Primary (citable) accession number: P31321 | ||||||||
| Entry history |
| ||||||||
| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation project | HPI (Human Proteome Initiative) | ||||||||
Relevant documents
| Human chromosome 7 Human chromosome 7: entries, gene names and cross-references to MIM |
| MIM cross-references Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot |
| SIMILARITY comments Index of protein domains and families |

Clusters with


