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Protein

cAMP-dependent protein kinase type I-beta regulatory subunit

Gene

PRKAR1B

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Regulatory subunit of the cAMP-dependent protein kinases involved in cAMP signaling in cells.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei202cAMP 11
Binding sitei211cAMP 11
Binding sitei326cAMP 21
Binding sitei335cAMP 21

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi137 – 254cAMP 1Add BLAST118
Nucleotide bindingi255 – 381cAMP 2Add BLAST127

GO - Molecular functioni

  • cAMP binding Source: UniProtKB-KW
  • cAMP-dependent protein kinase inhibitor activity Source: BHF-UCL
  • cAMP-dependent protein kinase regulator activity Source: BHF-UCL
  • protein kinase A catalytic subunit binding Source: BHF-UCL

GO - Biological processi

Keywordsi

LigandcAMP, cAMP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-HSA-163615. PKA activation.
R-HSA-164378. PKA activation in glucagon signalling.
R-HSA-180024. DARPP-32 events.
R-HSA-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-HSA-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-HSA-5610787. Hedgehog 'off' state.
R-HSA-983231. Factors involved in megakaryocyte development and platelet production.

Names & Taxonomyi

Protein namesi
Recommended name:
cAMP-dependent protein kinase type I-beta regulatory subunit
Gene namesi
Name:PRKAR1B
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:9390. PRKAR1B.

Subcellular locationi

GO - Cellular componenti

  • cAMP-dependent protein kinase complex Source: UniProtKB
  • ciliary base Source: Reactome
  • cytosol Source: Reactome
  • plasma membrane Source: UniProtKB-SubCell

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Organism-specific databases

DisGeNETi5575.
MalaCardsiPRKAR1B.
OpenTargetsiENSG00000188191.
PharmGKBiPA33756.

Chemistry databases

GuidetoPHARMACOLOGYi1473.

Polymorphism and mutation databases

BioMutaiPRKAR1B.
DMDMi229463042.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00002053812 – 381cAMP-dependent protein kinase type I-beta regulatory subunitAdd BLAST380

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei3PhosphoserineCombined sources1
Disulfide bondi18Interchain (with C-39)By similarity
Modified residuei21Nitrated tyrosineCombined sources1
Disulfide bondi39Interchain (with C-18)By similarity
Modified residuei77PhosphoserineCombined sources1
Modified residuei83PhosphoserineCombined sources1
Modified residuei85PhosphothreonineBy similarity1
Modified residuei97Omega-N-methylarginineBy similarity1

Post-translational modificationi

The pseudophosphorylation site binds to the substrate-binding region of the catalytic chain, resulting in the inhibition of its activity.

Keywords - PTMi

Disulfide bond, Methylation, Nitration, Phosphoprotein

Proteomic databases

EPDiP31321.
MaxQBiP31321.
PaxDbiP31321.
PeptideAtlasiP31321.
PRIDEiP31321.

PTM databases

iPTMnetiP31321.
PhosphoSitePlusiP31321.

Expressioni

Tissue specificityi

Four types of regulatory chains are found: I-alpha, I-beta, II-alpha, and II-beta. Their expression varies among tissues and is in some cases constitutive and in others inducible.

Gene expression databases

BgeeiENSG00000188191.
CleanExiHS_PRKAR1B.
ExpressionAtlasiP31321. baseline and differential.
GenevisibleiP31321. HS.

Organism-specific databases

HPAiHPA026719.

Interactioni

Subunit structurei

The inactive holoenzyme is composed of two regulatory chains and two catalytic chains. Activation by cAMP releases the two active catalytic monomers and the regulatory dimer. Interacts with PRKX; regulates this cAMP-dependent protein kinase. Interacts with C2orf88/smAKAP; this interaction may target PRKAR1B to the plasma membrane.2 Publications

Binary interactionsi

Show more details

GO - Molecular functioni

  • protein kinase A catalytic subunit binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi111561. 48 interactors.
IntActiP31321. 76 interactors.
MINTiMINT-240609.
STRINGi9606.ENSP00000353415.

Structurei

Secondary structure

1381
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi19 – 24Combined sources6
Turni27 – 31Combined sources5
Helixi32 – 41Combined sources10
Helixi46 – 68Combined sources23

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
4DINX-ray3.70B1-381[»]
4F9KX-ray2.80A/B/C/D11-73[»]
ProteinModelPortaliP31321.
SMRiP31321.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 136Dimerization and phosphorylationAdd BLAST135

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi96 – 100Pseudophosphorylation motif5

Sequence similaritiesi

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1113. Eukaryota.
COG0664. LUCA.
GeneTreeiENSGT00530000062947.
HOGENOMiHOG000196669.
HOVERGENiHBG002025.
InParanoidiP31321.
KOiK04739.
OMAiDCIVHLC.
OrthoDBiEOG091G0F1K.
PhylomeDBiP31321.
TreeFamiTF314920.

Family and domain databases

Gene3Di2.60.120.10. 2 hits.
InterProiView protein in InterPro
IPR012198. cAMP_dep_PK_reg_su.
IPR003117. cAMP_dep_PK_reg_su_I/II_a/b.
IPR018490. cNMP-bd-like.
IPR018488. cNMP-bd_CS.
IPR000595. cNMP-bd_dom.
IPR014710. RmlC-like_jellyroll.
PfamiView protein in Pfam
PF00027. cNMP_binding. 2 hits.
PF02197. RIIa. 1 hit.
PIRSFiPIRSF000548. PK_regulatory. 1 hit.
SMARTiView protein in SMART
SM00100. cNMP. 2 hits.
SM00394. RIIa. 1 hit.
SUPFAMiSSF47391. SSF47391. 1 hit.
SSF51206. SSF51206. 2 hits.
PROSITEiView protein in PROSITE
PS00888. CNMP_BINDING_1. 2 hits.
PS00889. CNMP_BINDING_2. 2 hits.
PS50042. CNMP_BINDING_3. 2 hits.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31321-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MASPPACPSE EDESLKGCEL YVQLHGIQQV LKDCIVHLCI SKPERPMKFL
60 70 80 90 100
REHFEKLEKE ENRQILARQK SNSQSDSHDE EVSPTPPNPV VKARRRRGGV
110 120 130 140 150
SAEVYTEEDA VSYVRKVIPK DYKTMTALAK AISKNVLFAH LDDNERSDIF
160 170 180 190 200
DAMFPVTHIA GETVIQQGNE GDNFYVVDQG EVDVYVNGEW VTNISEGGSF
210 220 230 240 250
GELALIYGTP RAATVKAKTD LKLWGIDRDS YRRILMGSTL RKRKMYEEFL
260 270 280 290 300
SKVSILESLE KWERLTVADA LEPVQFEDGE KIVVQGEPGD DFYIITEGTA
310 320 330 340 350
SVLQRRSPNE EYVEVGRLGP SDYFGEIALL LNRPRAATVV ARGPLKCVKL
360 370 380
DRPRFERVLG PCSEILKRNI QRYNSFISLT V
Length:381
Mass (Da):43,073
Last modified:May 5, 2009 - v4
Checksum:i1ED7BFEC30897191
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti270A → R in AAC37564 (PubMed:1708242).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M65066 mRNA. Translation: AAC37564.1.
AK315951 mRNA. Translation: BAH14322.1.
AK315990 mRNA. Translation: BAH14361.1.
BC026734 mRNA. Translation: AAH26734.1.
BC036828 mRNA. Translation: AAH36828.2.
CCDSiCCDS34579.1.
PIRiJH0392. OKHUR1.
RefSeqiNP_001158230.1. NM_001164758.1.
NP_001158231.1. NM_001164759.1.
NP_001158232.1. NM_001164760.1.
NP_001158233.1. NM_001164761.1.
NP_001158234.1. NM_001164762.1.
NP_002726.1. NM_002735.2.
UniGeneiHs.520851.

Genome annotation databases

EnsembliENST00000360274; ENSP00000353415; ENSG00000188191.
ENST00000403562; ENSP00000385349; ENSG00000188191.
ENST00000406797; ENSP00000385749; ENSG00000188191.
ENST00000537384; ENSP00000440449; ENSG00000188191.
ENST00000544935; ENSP00000444487; ENSG00000188191.
GeneIDi5575.
KEGGihsa:5575.
UCSCiuc003siu.3. human.

Similar proteinsi

Entry informationi

Entry nameiKAP1_HUMAN
AccessioniPrimary (citable) accession number: P31321
Secondary accession number(s): Q8N422
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: May 5, 2009
Last modified: August 30, 2017
This is version 169 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families