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Protein

Ornithine carbamoyltransferase, mitochondrial

Gene

arg3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Ornithine carbamoyltransferase involved in the synthesis of arginine from glutamate via ornithine and the urea cycle.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase, mitochondrial (arg3)
  2. Argininosuccinate synthase (arg12)
  3. Probable argininosuccinate lyase (argx), Argininosuccinate lyase (arg7)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei114 – 1141Carbamoyl phosphateBy similarity
Binding sitei114 – 1141OrnithineBy similarity
Binding sitei141 – 1411Carbamoyl phosphateBy similarity
Binding sitei172 – 1721OrnithineBy similarity
Active sitei276 – 2761By similarity
Binding sitei303 – 3031Carbamoyl phosphateBy similarity
Binding sitei303 – 3031OrnithineBy similarity

GO - Molecular functioni

  • amino acid binding Source: InterPro
  • ornithine carbamoyltransferase activity Source: PomBase

GO - Biological processi

  • arginine biosynthetic process via ornithine Source: PomBase
  • ornithine metabolic process Source: PomBase
  • urea cycle Source: GO_Central
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Amino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-70635. Urea cycle.
UniPathwayiUPA00068; UER00112.

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase, mitochondrial (EC:2.1.3.3)
Alternative name(s):
Ornithine transcarbamylase
Short name:
OTCase
Gene namesi
Name:arg3
ORF Names:SPAC4G9.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4G9.10.
PomBaseiSPAC4G9.10. arg3.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • mitochondrial matrix Source: UniProtKB-SubCell
  • mitochondrion Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 327Ornithine carbamoyltransferase, mitochondrialPRO_0000020346
Transit peptidei1 – ?Mitochondrion

Proteomic databases

MaxQBiP31317.

Interactioni

Subunit structurei

Interacts with trx2.1 Publication

Protein-protein interaction databases

BioGridi280008. 44 interactions.
MINTiMINT-4688296.

Structurei

3D structure databases

ProteinModelPortaliP31317.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni63 – 675Ornithine and carbamoyl phosphate bindingBy similarity
Regioni141 – 1444Ornithine and carbamoyl phosphate bindingBy similarity
Regioni236 – 2405Ornithine bindingBy similarity
Regioni275 – 2784Ornithine bindingBy similarity

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000022686.
InParanoidiP31317.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiEOG092C3SF9.
PhylomeDBiP31317.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31317-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFKKFPRHL LSIRDLSRGE IVKLIDRSSE IKQAYKQNFQ NRRSVQMSGL
60 70 80 90 100
SSQNVAMIFS KRSTRTRVSV ESAVSCLGGN AMFLGKDDIQ LGVNESLYDT
110 120 130 140 150
SKVISSMVSG IVARVNKYSD VATLAKHASC PVINGLCDTF HPLQALADLL
160 170 180 190 200
TIKETFKSFD GLKVAWVGDA NNVLHDLMIA NAKVGIHTSV AKPKDVNVRD
210 220 230 240 250
DILSIVNEAA NENGSTFEIV NDPKVAVKNA DIVVTDTWIS MGQEAEKEQR
260 270 280 290 300
LKQFTGFQVT GEIMKLAKPS CKFMHCLPRH PEEVSDEVFY GENSLVFQEA
310 320
ENRKWTTVAV LEALLVNRGE ILPPASA
Length:327
Mass (Da):36,119
Last modified:July 1, 1993 - v1
Checksum:iBF0E83E3C0051884
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63577 Genomic DNA. Translation: CAA45133.1.
CU329670 Genomic DNA. Translation: CAA93560.1.
PIRiS22390. OWZP.
RefSeqiNP_593692.1. NM_001019124.2.

Genome annotation databases

EnsemblFungiiSPAC4G9.10.1; SPAC4G9.10.1:pep; SPAC4G9.10.
GeneIDi2543593.
KEGGispo:SPAC4G9.10.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63577 Genomic DNA. Translation: CAA45133.1.
CU329670 Genomic DNA. Translation: CAA93560.1.
PIRiS22390. OWZP.
RefSeqiNP_593692.1. NM_001019124.2.

3D structure databases

ProteinModelPortaliP31317.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi280008. 44 interactions.
MINTiMINT-4688296.

Proteomic databases

MaxQBiP31317.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC4G9.10.1; SPAC4G9.10.1:pep; SPAC4G9.10.
GeneIDi2543593.
KEGGispo:SPAC4G9.10.

Organism-specific databases

EuPathDBiFungiDB:SPAC4G9.10.
PomBaseiSPAC4G9.10. arg3.

Phylogenomic databases

HOGENOMiHOG000022686.
InParanoidiP31317.
KOiK00611.
OMAiEHPCQIL.
OrthoDBiEOG092C3SF9.
PhylomeDBiP31317.

Enzyme and pathway databases

UniPathwayiUPA00068; UER00112.
ReactomeiR-SPO-70635. Urea cycle.

Miscellaneous databases

PROiP31317.

Family and domain databases

Gene3Di3.40.50.1370. 2 hits.
InterProiIPR006132. Asp/Orn_carbamoyltranf_P-bd.
IPR006130. Asp/Orn_carbamoylTrfase.
IPR006131. Asp_carbamoyltransf_Asp/Orn-bd.
IPR002292. Orn/put_carbamltrans.
[Graphical view]
PfamiPF00185. OTCace. 1 hit.
PF02729. OTCace_N. 1 hit.
[Graphical view]
PRINTSiPR00100. AOTCASE.
PR00102. OTCASE.
SUPFAMiSSF53671. SSF53671. 1 hit.
TIGRFAMsiTIGR00658. orni_carb_tr. 1 hit.
PROSITEiPS00097. CARBAMOYLTRANSFERASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiOTC_SCHPO
AccessioniPrimary (citable) accession number: P31317
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: September 7, 2016
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.