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Protein

Ornithine carbamoyltransferase, mitochondrial

Gene

arg3

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ornithine carbamoyltransferase involved in the synthesis of arginine from glutamate via ornithine and the urea cycle.1 Publication

Catalytic activityi

Carbamoyl phosphate + L-ornithine = phosphate + L-citrulline.

Pathwayi: L-arginine biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate.
Proteins known to be involved in the 3 steps of the subpathway in this organism are:
  1. Ornithine carbamoyltransferase, mitochondrial (arg3)
  2. Argininosuccinate synthase (arg12)
  3. Probable argininosuccinate lyase (argx), Argininosuccinate lyase (arg7)
This subpathway is part of the pathway L-arginine biosynthesis, which is itself part of Amino-acid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes L-arginine from L-ornithine and carbamoyl phosphate, the pathway L-arginine biosynthesis and in Amino-acid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei114Carbamoyl phosphateBy similarity1
Binding sitei114OrnithineBy similarity1
Binding sitei141Carbamoyl phosphateBy similarity1
Binding sitei172OrnithineBy similarity1
Active sitei276By similarity1
Binding sitei303Carbamoyl phosphateBy similarity1
Binding sitei303OrnithineBy similarity1

GO - Molecular functioni

  • amino acid binding Source: InterPro
  • ornithine carbamoyltransferase activity Source: PomBase

GO - Biological processi

  • arginine biosynthetic process via ornithine Source: PomBase
  • urea cycle Source: GO_Central

Keywordsi

Molecular functionTransferase
Biological processAmino-acid biosynthesis, Arginine biosynthesis

Enzyme and pathway databases

ReactomeiR-SPO-1268020 Mitochondrial protein import
R-SPO-70635 Urea cycle
UniPathwayiUPA00068; UER00112

Names & Taxonomyi

Protein namesi
Recommended name:
Ornithine carbamoyltransferase, mitochondrial (EC:2.1.3.3)
Alternative name(s):
Ornithine transcarbamylase
Short name:
OTCase
Gene namesi
Name:arg3
ORF Names:SPAC4G9.10
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC4G9.10
PomBaseiSPAC4G9.10 arg3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000020346? – 327Ornithine carbamoyltransferase, mitochondrial
Transit peptidei1 – ?Mitochondrion

Proteomic databases

MaxQBiP31317
PaxDbiP31317
PRIDEiP31317

Interactioni

Subunit structurei

Interacts with trx2.1 Publication

Protein-protein interaction databases

BioGridi280008, 44 interactors
STRINGi4896.SPAC4G9.10.1

Structurei

3D structure databases

ProteinModelPortaliP31317
SMRiP31317
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni63 – 67Ornithine and carbamoyl phosphate bindingBy similarity5
Regioni141 – 144Ornithine and carbamoyl phosphate bindingBy similarity4
Regioni236 – 240Ornithine bindingBy similarity5
Regioni275 – 278Ornithine bindingBy similarity4

Sequence similaritiesi

Belongs to the ATCase/OTCase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

HOGENOMiHOG000022686
InParanoidiP31317
KOiK00611
OMAiDGNNVCN
OrthoDBiEOG092C3SF9
PhylomeDBiP31317

Family and domain databases

Gene3Di3.40.50.1370, 3 hits
InterProiView protein in InterPro
IPR006132 Asp/Orn_carbamoyltranf_P-bd
IPR006130 Asp/Orn_carbamoylTrfase
IPR036901 Asp/Orn_carbamoylTrfase_sf
IPR006131 Asp_carbamoyltransf_Asp/Orn-bd
IPR002292 Orn/put_carbamltrans
PfamiView protein in Pfam
PF00185 OTCace, 1 hit
PF02729 OTCace_N, 1 hit
PRINTSiPR00100 AOTCASE
PR00102 OTCASE
SUPFAMiSSF53671 SSF53671, 1 hit
TIGRFAMsiTIGR00658 orni_carb_tr, 1 hit
PROSITEiView protein in PROSITE
PS00097 CARBAMOYLTRANSFERASE, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31317-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSFKKFPRHL LSIRDLSRGE IVKLIDRSSE IKQAYKQNFQ NRRSVQMSGL
60 70 80 90 100
SSQNVAMIFS KRSTRTRVSV ESAVSCLGGN AMFLGKDDIQ LGVNESLYDT
110 120 130 140 150
SKVISSMVSG IVARVNKYSD VATLAKHASC PVINGLCDTF HPLQALADLL
160 170 180 190 200
TIKETFKSFD GLKVAWVGDA NNVLHDLMIA NAKVGIHTSV AKPKDVNVRD
210 220 230 240 250
DILSIVNEAA NENGSTFEIV NDPKVAVKNA DIVVTDTWIS MGQEAEKEQR
260 270 280 290 300
LKQFTGFQVT GEIMKLAKPS CKFMHCLPRH PEEVSDEVFY GENSLVFQEA
310 320
ENRKWTTVAV LEALLVNRGE ILPPASA
Length:327
Mass (Da):36,119
Last modified:July 1, 1993 - v1
Checksum:iBF0E83E3C0051884
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X63577 Genomic DNA Translation: CAA45133.1
CU329670 Genomic DNA Translation: CAA93560.1
PIRiS22390 OWZP
RefSeqiNP_593692.1, NM_001019124.2

Genome annotation databases

EnsemblFungiiSPAC4G9.10.1; SPAC4G9.10.1:pep; SPAC4G9.10
GeneIDi2543593
KEGGispo:SPAC4G9.10

Similar proteinsi

Entry informationi

Entry nameiOTC_SCHPO
AccessioniPrimary (citable) accession number: P31317
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 141 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health