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Protein

Homeobox protein Hox-A10

Gene

Hoxa10

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Sequence-specific transcription factor which is part of a developmental regulatory system that provides cells with specific positional identities on the anterior-posterior axis. Binds to the DNA sequence 5'-AA[AT]TTTTATTAC-3'.

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
DNA bindingi342 – 40160HomeoboxPROSITE-ProRule annotationAdd
BLAST

GO - Molecular functioni

GO - Biological processi

  • anterior/posterior pattern specification Source: MGI
  • embryonic limb morphogenesis Source: MGI
  • male gonad development Source: MGI
  • multicellular organism development Source: MGI
  • positive regulation of transcription from RNA polymerase II promoter Source: MGI
  • proximal/distal pattern formation Source: MGI
  • regulation of gene expression Source: MGI
  • single fertilization Source: MGI
  • skeletal system development Source: MGI
  • spermatogenesis Source: MGI
  • transcription from RNA polymerase II promoter Source: MGI
  • uterus development Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein

Keywords - Biological processi

Transcription, Transcription regulation

Keywords - Ligandi

DNA-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Homeobox protein Hox-A10
Alternative name(s):
Homeobox protein Hox-1.8
Gene namesi
Name:Hoxa10
Synonyms:Hox-1.8, Hoxa-10
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:96171. Hoxa10.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: UniProtKB
  • nucleus Source: UniProtKB
  • transcription factor complex Source: MGI
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 416416Homeobox protein Hox-A10PRO_0000200091Add
BLAST

Proteomic databases

MaxQBiP31310.
PaxDbiP31310.
PRIDEiP31310.

PTM databases

PhosphoSiteiP31310.

Expressioni

Tissue specificityi

Expressed in the developing limb bud where it is restricted to the mesenchyme along the proximal-distal axis. Also found in developing gut and urogenital tract. In adult tissue, both forms found in kidney but only isoform 1 is expressed in skeletal muscle.

Developmental stagei

Embryonic expression increases from day 9 to day 12 and then declines to day 15.

Gene expression databases

BgeeiP31310.
CleanExiMM_HOXA10.
GenevisibleiP31310. MM.

Interactioni

Subunit structurei

Interacts with SIRT2; the interaction is direct.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi200363. 6 interactions.
IntActiP31310. 3 interactions.
MINTiMINT-1172417.
STRINGi10090.ENSMUSP00000120276.

Structurei

3D structure databases

ProteinModelPortaliP31310.
SMRiP31310. Positions 343-401.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi43 – 5513Gly-richAdd
BLAST
Compositional biasi94 – 10714Poly-GlyAdd
BLAST
Compositional biasi130 – 16132Gln/Pro-richAdd
BLAST
Compositional biasi232 – 2398Poly-Gly
Compositional biasi288 – 2914Poly-Gly

Sequence similaritiesi

Belongs to the Abd-B homeobox family.Curated
Contains 1 homeobox DNA-binding domain.PROSITE-ProRule annotation

Keywords - Domaini

Homeobox

Phylogenomic databases

eggNOGiKOG0487. Eukaryota.
ENOG4111FJP. LUCA.
GeneTreeiENSGT00760000118834.
HOGENOMiHOG000231182.
HOVERGENiHBG052934.
InParanoidiP31310.
KOiK17443.
OMAiCRMEPPE.
OrthoDBiEOG72JWGM.
PhylomeDBiP31310.
TreeFamiTF317819.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P31310-1) [UniParc]FASTAAdd to basket

Also known as: a10-1

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MSARKGYLLP SPNYPTTMSC SESPAANSFL VDSLISSGRG EAGVGGGSAG
60 70 80 90 100
GGGGGYYAHG GVYLPPASDL PYGLQSCGLF PALGSKRNEA PSPGGGGGGG
110 120 130 140 150
SGGLGPGTHG YAPAPLDLWL DAPRSCRMEP PDGPPPPQPQ PQQQQQQPPP
160 170 180 190 200
PPPQPPQPQP QATSCSFAQN IKEESSYCLY DAADKCPKGS AAADLAPFPR
210 220 230 240 250
GPPPDGCALG ASSGVPVPGY FRLSQAYGTA KGFGSGGGGT QQLASPFPAQ
260 270 280 290 300
PPGRGFDPPP ALASGSTEAA GKERVLDSTP PPTLVCTGGG GSQGDEEAHA
310 320 330 340 350
SSSAAEELSP APSENSKASP EKDSLGSSKG ENAANWLTAK SGRKKRCPYT
360 370 380 390 400
KHQTLELEKE FLFNMYLTRE RRLEISRSVH LTDRQVKIWF QNRRMKLKKM
410
NRENRIRELT ANFNFS
Length:416
Mass (Da):43,292
Last modified:April 20, 2010 - v4
Checksum:iA088014205877F3E
GO
Isoform 2 (identifier: P31310-2) [UniParc]FASTAAdd to basket

Also known as: a10-2

The sequence of this isoform differs from the canonical sequence as follows:
     1-322: Missing.
     323-325: DSL → MSQ

Show »
Length:94
Mass (Da):11,409
Checksum:i045A4859DBED931E
GO

Sequence cautioni

The sequence AAA67125.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated
The sequence AAH50839.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally extended.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti44 – 441V → G in AAH50839 (PubMed:15489334).Curated
Sequence conflicti241 – 2411Q → E in AAA67125 (PubMed:7862151).Curated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei1 – 322322Missing in isoform 2. 1 PublicationVSP_002386Add
BLAST
Alternative sequencei323 – 3253DSL → MSQ in isoform 2. 1 PublicationVSP_002387

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08757 mRNA. Translation: AAA67125.1. Different initiation.
L08758 mRNA. Translation: AAD15230.1.
BC050839 mRNA. Translation: AAH50839.1. Different initiation.
M81659 Genomic DNA. Translation: AAA63312.1.
CCDSiCCDS20147.2. [P31310-1]
CCDS51775.1. [P31310-2]
PIRiI49754.
RefSeqiNP_001116422.1. NM_001122950.2. [P31310-2]
NP_032289.2. NM_008263.3. [P31310-1]
UniGeneiMm.5.

Genome annotation databases

EnsembliENSMUST00000121043; ENSMUSP00000112872; ENSMUSG00000000938. [P31310-2]
ENSMUST00000125581; ENSMUSP00000120276; ENSMUSG00000000938. [P31310-1]
GeneIDi15395.
KEGGimmu:15395.
UCSCiuc009bym.1. mouse. [P31310-1]
uc012ema.1. mouse. [P31310-2]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L08757 mRNA. Translation: AAA67125.1. Different initiation.
L08758 mRNA. Translation: AAD15230.1.
BC050839 mRNA. Translation: AAH50839.1. Different initiation.
M81659 Genomic DNA. Translation: AAA63312.1.
CCDSiCCDS20147.2. [P31310-1]
CCDS51775.1. [P31310-2]
PIRiI49754.
RefSeqiNP_001116422.1. NM_001122950.2. [P31310-2]
NP_032289.2. NM_008263.3. [P31310-1]
UniGeneiMm.5.

3D structure databases

ProteinModelPortaliP31310.
SMRiP31310. Positions 343-401.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi200363. 6 interactions.
IntActiP31310. 3 interactions.
MINTiMINT-1172417.
STRINGi10090.ENSMUSP00000120276.

PTM databases

PhosphoSiteiP31310.

Proteomic databases

MaxQBiP31310.
PaxDbiP31310.
PRIDEiP31310.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000121043; ENSMUSP00000112872; ENSMUSG00000000938. [P31310-2]
ENSMUST00000125581; ENSMUSP00000120276; ENSMUSG00000000938. [P31310-1]
GeneIDi15395.
KEGGimmu:15395.
UCSCiuc009bym.1. mouse. [P31310-1]
uc012ema.1. mouse. [P31310-2]

Organism-specific databases

CTDi3206.
MGIiMGI:96171. Hoxa10.

Phylogenomic databases

eggNOGiKOG0487. Eukaryota.
ENOG4111FJP. LUCA.
GeneTreeiENSGT00760000118834.
HOGENOMiHOG000231182.
HOVERGENiHBG052934.
InParanoidiP31310.
KOiK17443.
OMAiCRMEPPE.
OrthoDBiEOG72JWGM.
PhylomeDBiP31310.
TreeFamiTF317819.

Miscellaneous databases

NextBioi288076.
PROiP31310.
SOURCEiSearch...

Gene expression databases

BgeeiP31310.
CleanExiMM_HOXA10.
GenevisibleiP31310. MM.

Family and domain databases

Gene3Di1.10.10.60. 1 hit.
InterProiIPR017970. Homeobox_CS.
IPR001356. Homeobox_dom.
IPR020479. Homeobox_metazoa.
IPR009057. Homeodomain-like.
[Graphical view]
PfamiPF00046. Homeobox. 1 hit.
[Graphical view]
PRINTSiPR00024. HOMEOBOX.
SMARTiSM00389. HOX. 1 hit.
[Graphical view]
SUPFAMiSSF46689. SSF46689. 1 hit.
PROSITEiPS00027. HOMEOBOX_1. 1 hit.
PS50071. HOMEOBOX_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The expression pattern of the murine Hoxa-10 gene and the sequence recognition of its homeodomain reveal specific properties of Abdominal B-like genes."
    Benson G.V., Nguyen T.-H.E., Maas R.L.
    Mol. Cell. Biol. 15:1591-1601(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORMS 1 AND 2).
    Strain: CD-1.
    Tissue: Kidney.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 9-416 (ISOFORM 1).
    Tissue: Limb.
  3. Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 342-401.
  4. "Detection of homeobox genes in development and evolution."
    Murtha M.T., Leckman J.F., Ruddle F.H.
    Proc. Natl. Acad. Sci. U.S.A. 88:10711-10715(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 363-387.
    Strain: C57BL/6J.
    Tissue: Spleen.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Kidney.

Entry informationi

Entry nameiHXA10_MOUSE
AccessioniPrimary (citable) accession number: P31310
Secondary accession number(s): Q80Y22
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: April 20, 2010
Last modified: May 11, 2016
This is version 143 of the entry and version 4 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Vertebrate homeotic Hox proteins
    Nomenclature of vertebrate homeotic Hox proteins and list of entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.