Reviewed, UniProtKB/Swiss-Prot P31300 (CYSKP_CAPAN)

Names and origin

Protein names	Recommended name:     Cysteine synthase, chloroplastic/chromoplastic     EC=2.5.1.47 Alternative name(s):     O-acetylserine sulfhydrylase     CSase B       Short name=CS-B     O-acetylserine (thiol)-lyase     OAS-TL B
Organism	Capsicum annuum (Bell pepper)
Taxonomic identifier	4072 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Capsiceae › Capsicum

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	O(3)-acetyl-L-serine + H2S = L-cysteine + acetate.
Cofactor	Pyridoxal phosphate.
Pathway	Amino-acid biosynthesis; L-cysteine biosynthesis; L-cysteine from L-serine: step 2/2.
Subunit structure	Homodimer.
Subcellular location	Plastid › chloroplast stroma. Plastid › chromoplast.
Sequence similarities	Belongs to the cysteine synthase/cystathionine beta-synthase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Cysteine biosynthesis
Cellular component	Chloroplast Chromoplast Plastid
Domain	Transit peptide
Ligand	Pyridoxal phosphate
Molecular function	Transferase
Gene Ontology (GO)
Biological process	cysteine biosynthetic process from serine Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast stroma Inferred from electronic annotation. Source: UniProtKB-SubCell chromoplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cysteine synthase activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro transferase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Chloroplast and chromoplast By similarity
	Chain	51 – 374	324	Cysteine synthase, chloroplastic/chromoplastic		PRO_0000006350
Regions
	Region	243 – 247	5	Pyridoxal phosphate binding By similarity
Sites
	Binding site	139	1	Pyridoxal phosphate By similarity
	Binding site	321	1	Pyridoxal phosphate By similarity
Amino acid modifications
	Modified residue	108	1	N6-(pyridoxal phosphate)lysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31300-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 36A6D0CDCCEA23F1	FASTA	374	39,978
	10 20 30 40 50 60 MASIINNPFT SLCCNTNKCE PNRICSLRSQ QSLVFDNVNR KVGFPSVVCK AVSVQTKSPT 70 80 90 100 110 120 EIEGLNIAED VTQLIGNTPM VYLNTIVKGC VANIAAKLEI MEPCCSVKDR IGFSMISDAE 130 140 150 160 170 180 EKGLISPGKT VLVEPTSGNT GIGLAFIAAS RGYKLILTMP ASMSLERRVI LKAFGAELVL 190 200 210 220 230 240 TDPAKGMKGA VSKAEEILNN TPDAYILQQF DNPANPKIHY ETTGPEIWED TKGKIDILVA 250 260 270 280 290 300 GIGTGGTISG TGRYLKEKNP NIKIIGVEPT ESNVLSGGKP GFIPGNLDQD VMDEVIEISS 310 320 330 340 350 360 DEAVETAKQL ALQEGLLVGI SSGAAALAAI QVAKRPENAG KLIAVVFPSF GERYLSSILF 370 QSIREECEKM KPEL

References

[1]

"Cysteine synthase from Capsicum annuum chromoplasts. Characterization and cDNA cloning of an up-regulated enzyme during fruit development." Roemer S., D'Harlingue A., Camara B., Schantz R., Kuntz M. J. Biol. Chem. 267:17966-17970(1992) [PubMed: 1381358] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Lamuyo. Tissue: Fruit.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X64874 mRNA. Translation: CAA46086.1.
PIR	A43407.
3D structure databases
HSSP	HSSP built from PDB template 1J6N based on UniProtKB Q9WZD3.
SMR	P31300. Positions 67-373.
ModBase	Search...
Enzyme and pathway databases
BRENDA	2.5.1.47. 1261.
Family and domain databases
InterPro	IPR001216. Cys_synth_BS. IPR005856. Cys_synthKM. IPR005859. CysK. IPR001926. PyrdxlP-dep_enz_bsu. [Graphical view]
Pfam	PF00291. PALP. 1 hit. [Graphical view]
TIGRFAMs	TIGR01139. cysK. 1 hit. TIGR01136. cysKM. 1 hit.
PROSITE	PS00901. CYS_SYNTHASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CYSKP_CAPAN

Accession

Primary (citable) accession number: P31300

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 68 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)