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Protein

DNA repair protein RAD16

Gene

RAD16

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Component of the global genome repair (GGR) complex which promotes global genome nucleotide excision repair (GG-NER) which removes DNA damage from nontranscribing DNA. Involved in differential repair of DNA after UV damage. Will repair preferentially the MAT-alpha locus compared with the HML-alpha locus.2 Publications

Miscellaneous

Present with 358 molecules/cell in log phase SD medium.1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi210 – 217ATPPROSITE-ProRule annotation8
Zinc fingeri537 – 581RING-typePROSITE-ProRule annotationAdd BLAST45

GO - Molecular functioni

GO - Biological processi

  • cellular protein localization Source: SGD
  • nucleotide-excision repair, DNA damage recognition Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD

Keywordsi

Molecular functionDNA-binding, Helicase, Hydrolase
Biological processDNA damage, DNA repair
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciYEAST:G3O-29074-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA repair protein RAD16 (EC:3.6.4.-)
Alternative name(s):
ATP-dependent helicase RAD16
Gene namesi
Name:RAD16
Ordered Locus Names:YBR114W
ORF Names:YBR0909
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome II

Organism-specific databases

EuPathDBiFungiDB:YBR114W.
SGDiS000000318. RAD16.

Subcellular locationi

GO - Cellular componenti

  • Cul3-RING ubiquitin ligase complex Source: SGD
  • nucleotide-excision repair factor 4 complex Source: SGD

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000561321 – 790DNA repair protein RAD16Add BLAST790

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei25PhosphoserineCombined sources1
Modified residuei109PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31244.
PRIDEiP31244.

PTM databases

iPTMnetiP31244.

Interactioni

Subunit structurei

Component of the global genome repair (GGR) complex composed of at least ABF1, RAD7 and RAD16.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
RAD7P067794EBI-14645,EBI-14780

Protein-protein interaction databases

BioGridi32817. 108 interactors.
DIPiDIP-697N.
IntActiP31244. 26 interactors.
MINTiMINT-616306.
STRINGi4932.YBR114W.

Structurei

3D structure databases

ProteinModelPortaliP31244.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini197 – 371Helicase ATP-bindingPROSITE-ProRule annotationAdd BLAST175
Domaini623 – 777Helicase C-terminalPROSITE-ProRule annotationAdd BLAST155

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi322 – 325DEAH box4

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi8 – 13Poly-Arg6
Compositional biasi65 – 70Poly-Asp6
Compositional biasi125 – 129Poly-Lys5

Sequence similaritiesi

Belongs to the SNF2/RAD54 helicase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri537 – 581RING-typePROSITE-ProRule annotationAdd BLAST45

Keywords - Domaini

Zinc-finger

Phylogenomic databases

GeneTreeiENSGT00880000137978.
HOGENOMiHOG000179829.
InParanoidiP31244.
KOiK15083.
OMAiIFDRECI.
OrthoDBiEOG092C0L0F.

Family and domain databases

Gene3Di3.30.40.10. 1 hit.
InterProiView protein in InterPro
IPR014001. Helicase_ATP-bd.
IPR001650. Helicase_C.
IPR027417. P-loop_NTPase.
IPR000330. SNF2_N.
IPR018957. Znf_C3HC4_RING-type.
IPR001841. Znf_RING.
IPR013083. Znf_RING/FYVE/PHD.
IPR017907. Znf_RING_CS.
PfamiView protein in Pfam
PF00271. Helicase_C. 1 hit.
PF00176. SNF2_N. 1 hit.
PF00097. zf-C3HC4. 1 hit.
SMARTiView protein in SMART
SM00487. DEXDc. 1 hit.
SM00490. HELICc. 1 hit.
SM00184. RING. 1 hit.
SUPFAMiSSF52540. SSF52540. 4 hits.
PROSITEiView protein in PROSITE
PS51192. HELICASE_ATP_BIND_1. 1 hit.
PS51194. HELICASE_CTER. 1 hit.
PS00518. ZF_RING_1. 1 hit.
PS50089. ZF_RING_2. 1 hit.

Sequencei

Sequence statusi: Complete.

P31244-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQEGGFIRRR RTRSTKKSVN YNELSDDDTA VKNSKTLQLK GNSENVNDSQ
60 70 80 90 100
DEEYRDDATL VKSPDDDDKD FIIDLTGSDK ERTATDENTH AIKNDNDEII
110 120 130 140 150
EIKEERDVSD DDEPLTKKRK TTARKKKKKT STKKKSPKVT PYERNTLRLY
160 170 180 190 200
EHHPELRNVF TDLKNAPPYV PQRSKQPDGM TIKLLPFQLE GLHWLISQEE
210 220 230 240 250
SIYAGGVLAD EMGMGKTIQT IALLMNDLTK SPSLVVAPTV ALMQWKNEIE
260 270 280 290 300
QHTKGQLKIY IYHGASRTTD IKDLQGYDVV LTTYAVLESV FRKQNYGFRR
310 320 330 340 350
KNGLFKQPSV LHNIDFYRVI LDEAHNIKDR QSNTARAVNN LKTQKRWCLS
360 370 380 390 400
GTPLQNRIGE MYSLIRFLNI NPFTKYFCTK CDCASKDWKF TDRMHCDHCS
410 420 430 440 450
HVIMQHTNFF NHFMLKNIQK FGVEGPGLES FNNIQTLLKN IMLRRTKVER
460 470 480 490 500
ADDLGLPPRI VTVRRDFFNE EEKDLYRSLY TDSKRKYNSF VEEGVVLNNY
510 520 530 540 550
ANIFTLITRM RQLADHPDLV LKRLNNFPGD DIGVVICQLC NDEAEEPIES
560 570 580 590 600
KCHHKFCRLC IKEYVESFME NNNKLTCPVC HIGLSIDLSQ PALEVDLDSF
610 620 630 640 650
KKQSIVSRLN MSGKWQSSTK IEALVEELYK LRSNKRTIKS IVFSQFTSML
660 670 680 690 700
DLVEWRLKRA GFQTVKLQGS MSPTQRDETI KYFMNNIQCE VFLVSLKAGG
710 720 730 740 750
VALNLCEASQ VFILDPWWNP SVEWQSGDRV HRIGQYRPVK ITRFCIEDSI
760 770 780 790
EARIIELQEK KANMIHATIN QDEAAISRLT PADLQFLFNN
Length:790
Mass (Da):91,430
Last modified:July 1, 1993 - v1
Checksum:i954317B5E85C6306
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86929 Genomic DNA. Translation: AAA34931.1.
X66247 Genomic DNA. Translation: CAA46974.1.
X78993 Genomic DNA. Translation: CAA55616.1.
Z35983 Genomic DNA. Translation: CAA85071.1.
M83553 Genomic DNA. Translation: AAA34930.1.
BK006936 Genomic DNA. Translation: DAA07233.1.
PIRiS25366.
RefSeqiNP_009672.1. NM_001178462.1.

Genome annotation databases

EnsemblFungiiYBR114W; YBR114W; YBR114W.
GeneIDi852411.
KEGGisce:YBR114W.

Similar proteinsi

Entry informationi

Entry nameiRAD16_YEAST
AccessioniPrimary (citable) accession number: P31244
Secondary accession number(s): D6VQB3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: August 30, 2017
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome II
    Yeast (Saccharomyces cerevisiae) chromosome II: entries and gene names