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Protein

Platelet-derived growth factor subunit B

Gene

Pdgfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei108 – 1081Involved in receptor binding
Sitei111 – 1111Involved in receptor binding

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Enzyme and pathway databases

ReactomeiREACT_278498. Downstream signal transduction.
REACT_280178. Signaling by PDGF.
REACT_307071. Platelet degranulation.
REACT_320075. Non-integrin membrane-ECM interactions.
REACT_333474. PIP3 activates AKT signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-derived growth factor subunit B
Short name:
PDGF subunit B
Alternative name(s):
PDGF-2
Platelet-derived growth factor B chain
Platelet-derived growth factor beta polypeptide
Proto-oncogene c-Sis
Gene namesi
Name:Pdgfb
Synonyms:Sis
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97528. Pdgfb.

Subcellular locationi

  • Secreted

  • Note: Released by platelets upon wounding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Perinatal lethality, due to severe hemorrhages shortly before birth. Kidney glomerular tufts do not form, apparently because of absence of mesangial cells. The heart and some large arteries are dilated in late-stage embryos. Mice lack microvascular pericytes, which normally form part of the capillary wall, and develop numerous capillary microaneurysms, leading to hemorrhages. Mice display erythroblastosis, macrocytic anemia, and thrombocytopenia.4 Publications

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Propeptidei21 – 8161Removed in mature formBy similarityPRO_0000023374Add
BLAST
Chaini82 – 190109Platelet-derived growth factor subunit BPRO_0000023375Add
BLAST
Propeptidei191 – 24151Removed in mature formBy similarityPRO_0000023376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi97 ↔ 141By similarity
Disulfide bondi124 – 124InterchainBy similarity
Disulfide bondi130 ↔ 178By similarity
Disulfide bondi133 – 133InterchainBy similarity
Disulfide bondi134 ↔ 180By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP31240.
PRIDEiP31240.

PTM databases

PhosphoSiteiP31240.

Expressioni

Tissue specificityi

Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4.1 Publication

Gene expression databases

BgeeiP31240.
CleanExiMM_PDGFB.
GenevisibleiP31240. MM.

Interactioni

Subunit structurei

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 (By similarity).By similarity

Protein-protein interaction databases

IntActiP31240. 1 interaction.
STRINGi10090.ENSMUSP00000000500.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DZ4model-A90-185[»]
2DZ5model-A90-185[»]
ProteinModelPortaliP31240.
SMRiP31240. Positions 25-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG72843.
HOGENOMiHOG000286027.
HOVERGENiHBG053546.
InParanoidiP31240.
KOiK17386.
OrthoDBiEOG78H3VV.
PhylomeDBiP31240.
TreeFamiTF319554.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRCWALFLP LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHR
60 70 80 90 100
DSVDEDGAEL DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR
110 120 130 140 150
TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP
160 170 180 190 200
VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT RSPGTSREQR
210 220 230 240
AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A
Length:241
Mass (Da):27,382
Last modified:July 1, 1993 - v1
Checksum:i3C5EB7A2DAD64178
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831I → V in AAH53430 (PubMed:15489334).Curated
Sequence conflicti183 – 1831I → V in AAH64056 (PubMed:15489334).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84453
, M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
M64849
, M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
BC053430 mRNA. Translation: AAH53430.1.
BC064056 mRNA. Translation: AAH64056.1.
CCDSiCCDS27656.1.
PIRiA39073. PFMSGB.
RefSeqiNP_035187.2. NM_011057.3.
UniGeneiMm.144089.

Genome annotation databases

GeneIDi18591.
KEGGimmu:18591.
UCSCiuc007wuz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84453
, M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
M64849
, M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
BC053430 mRNA. Translation: AAH53430.1.
BC064056 mRNA. Translation: AAH64056.1.
CCDSiCCDS27656.1.
PIRiA39073. PFMSGB.
RefSeqiNP_035187.2. NM_011057.3.
UniGeneiMm.144089.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DZ4model-A90-185[»]
2DZ5model-A90-185[»]
ProteinModelPortaliP31240.
SMRiP31240. Positions 25-182.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP31240. 1 interaction.
STRINGi10090.ENSMUSP00000000500.

PTM databases

PhosphoSiteiP31240.

Proteomic databases

MaxQBiP31240.
PRIDEiP31240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18591.
KEGGimmu:18591.
UCSCiuc007wuz.1. mouse.

Organism-specific databases

CTDi5155.
MGIiMGI:97528. Pdgfb.

Phylogenomic databases

eggNOGiNOG72843.
HOGENOMiHOG000286027.
HOVERGENiHBG053546.
InParanoidiP31240.
KOiK17386.
OrthoDBiEOG78H3VV.
PhylomeDBiP31240.
TreeFamiTF319554.

Enzyme and pathway databases

ReactomeiREACT_278498. Downstream signal transduction.
REACT_280178. Signaling by PDGF.
REACT_307071. Platelet degranulation.
REACT_320075. Non-integrin membrane-ECM interactions.
REACT_333474. PIP3 activates AKT signaling.

Miscellaneous databases

NextBioi294476.
PROiP31240.
SOURCEiSearch...

Gene expression databases

BgeeiP31240.
CleanExiMM_PDGFB.
GenevisibleiP31240. MM.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the murine c-sis proto-oncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor."
    Bonthron D.T., Sultan P., Collins T.
    Genomics 10:287-292(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Mammary gland.
  3. "Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities."
    Leveen P., Pekny M., Gebre-Medhin S., Swolin B., Larsson E., Betsholtz C.
    Genes Dev. 8:1875-1887(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  4. "Pericyte loss and microaneurysm formation in PDGF-B-deficient mice."
    Lindahl P., Johansson B.R., Leveen P., Betsholtz C.
    Science 277:242-245(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "Platelet-derived growth factor receptor beta regulates migration and DNA synthesis in metanephric mesenchymal cells."
    Arar M., Xu Y.C., Elshihabi I., Barnes J.L., Choudhury G.G., Abboud H.E.
    J. Biol. Chem. 275:9527-9533(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF beta-receptor null mice."
    Kaminski W.E., Lindahl P., Lin N.L., Broudy V.C., Crosby J.R., Hellstrom M., Swolin B., Bowen-Pope D.F., Martin P.J., Ross R., Betsholtz C., Raines E.W.
    Blood 97:1990-1998(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury."
    Taneda S., Hudkins K.L., Topouzis S., Gilbertson D.G., Ophascharoensuk V., Truong L., Johnson R.J., Alpers C.E.
    J. Am. Soc. Nephrol. 14:2544-2555(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors."
    Abramsson A., Lindblom P., Betsholtz C.
    J. Clin. Invest. 112:1142-1151(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: REVIEW.
  10. "Insight into the physiological functions of PDGF through genetic studies in mice."
    Betsholtz C.
    Cytokine Growth Factor Rev. 15:215-228(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON FUNCTION, DISRUPTION PHENOTYPE.
  11. "Comprehensive dissection of PDGF-PDGFR signaling pathways in PDGFR genetically defined cells."
    Wu E., Palmer N., Tian Z., Moseman A.P., Galdzicki M., Wang X., Berger B., Zhang H., Kohane I.S.
    PLoS ONE 3:E3794-E3794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPDGFB_MOUSE
AccessioniPrimary (citable) accession number: P31240
Secondary accession number(s): Q6P3C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 24, 2015
This is version 132 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.