P31240 (PDGFB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Platelet-derived growth factor subunit B
Short name=PDGF subunit B

Alternative name(s):
PDGF-2
Platelet-derived growth factor B chain
Platelet-derived growth factor beta polypeptide
Proto-oncogene c-Sis

Gene names

Name:	Pdgfb
Synonyms:	Sis

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	241 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. Ref.3 Ref.5 Ref.8 Ref.11
Subunit structure	Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 By similarity.
Subcellular location	Secreted. Note: Released by platelets upon wounding By similarity.
Tissue specificity	Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4. Ref.7
Disruption phenotype	Perinatal lethality, due to severe hemorrhages shortly before birth. Kidney glomerular tufts do not form, apparently because of absence of mesangial cells. The heart and some large arteries are dilated in late-stage embryos. Mice lack microvascular pericytes, which normally form part of the capillary wall, and develop numerous capillary microaneurysms, leading to hemorrhages. Mice display erythroblastosis, macrocytic anemia, and thrombocytopenia. Ref.3 Ref.4 Ref.6 Ref.10
Sequence similarities	Belongs to the PDGF/VEGF growth factor family.

Ontologies

Keywords
Cellular component	Secreted
Disease	Proto-oncogene
Domain	Signal
Molecular function	Developmental protein Growth factor Mitogen
PTM	Cleavage on pair of basic residues Disulfide bond Glycoprotein
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA replication Inferred from electronic annotation. Source: Compara actin cytoskeleton organization Inferred from direct assay PubMed 12176888. Source: MGI activation of protein kinase B activity Inferred from sequence or structural similarity. Source: UniProtKB blood vessel morphogenesis Inferred from direct assay PubMed 15919073. Source: MGI branching involved in salivary gland morphogenesis Inferred from mutant phenotype PubMed 18559345. Source: MGI cardiac vascular smooth muscle cell differentiation Traceable author statement PubMed 20299672. Source: DFLAT cell growth Inferred from electronic annotation. Source: Compara cell projection assembly Inferred from direct assay PubMed 12176888. Source: MGI cellular response to mycophenolic acid Inferred from sequence or structural similarity. Source: UniProtKB embryonic placenta development Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB epithelial cell proliferation involved in salivary gland morphogenesis Inferred from mutant phenotype PubMed 18559345. Source: MGI metanephric glomerular endothelium development Inferred from expression pattern PubMed 9693135. Source: UniProtKB metanephric glomerular mesangial cell development Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB metanephric glomerular mesangial cell proliferation involved in metanephros development Inferred from mutant phenotype PubMed 9693135. Source: UniProtKB monocyte chemotaxis Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of cell migration Inferred from genetic interaction PubMed 16644864. Source: MGI negative regulation of phosphatidylinositol biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of platelet activation Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB negative regulation of vasodilation Traceable author statement PubMed 20299672. Source: DFLAT paracrine signaling Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB peptidyl-serine phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB peptidyl-tyrosine phosphorylation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of DNA biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of DNA replication Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of ERK1 and ERK2 cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of MAP kinase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of blood vessel endothelial cell migration Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of calcium ion import Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of cell division Inferred from electronic annotation. Source: UniProtKB-KW positive regulation of cyclin-dependent protein kinase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway Traceable author statement PubMed 18663143. Source: BHF-UCL positive regulation of endothelial cell proliferation Inferred from genetic interaction PubMed 16644864. Source: MGI positive regulation of fibroblast growth factor receptor signaling pathway Inferred from direct assay PubMed 18559345. Source: MGI positive regulation of fibroblast proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of glomerular filtration Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB positive regulation of glomerular mesangial cell proliferation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of hyaluronan biosynthetic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of mitosis Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of phosphatidylinositol 3-kinase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of phosphatidylinositol 3-kinase cascade Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein autophosphorylation Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of protein tyrosine kinase activity Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of reactive oxygen species metabolic process Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of smooth muscle cell migration Inferred from mutant phenotype PubMed 10375497. Source: UniProtKB positive regulation of smooth muscle cell proliferation Inferred from mutant phenotype PubMed 10375497. Source: UniProtKB positive regulation of transcription, DNA-dependent Inferred from sequence or structural similarity. Source: UniProtKB positive regulation of vasoconstriction Traceable author statement PubMed 20299672. Source: DFLAT reactive oxygen species metabolic process Inferred from sequence or structural similarity. Source: UniProtKB response to drug Inferred from electronic annotation. Source: Compara response to estradiol stimulus Inferred from electronic annotation. Source: Compara response to hypoxia Inferred from electronic annotation. Source: Compara response to insulin stimulus Inferred from electronic annotation. Source: Compara response to wounding Inferred from sequence or structural similarity. Source: UniProtKB substrate-dependent cell migration Inferred from direct assay PubMed 15183724. Source: MGI transforming growth factor beta receptor signaling pathway Inferred from sequence or structural similarity. Source: UniProtKB wound healing Inferred from electronic annotation. Source: Compara
Cellular_component	basolateral plasma membrane Inferred from sequence or structural similarity. Source: UniProtKB cell surface Inferred from sequence or structural similarity. Source: UniProtKB cytoplasm Inferred from sequence or structural similarity. Source: UniProtKB extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	eukaryotic cell surface binding Inferred from sequence or structural similarity. Source: UniProtKB platelet-derived growth factor receptor binding Inferred from sequence or structural similarity. Source: UniProtKB protein homodimerization activity Inferred from sequence or structural similarity. Source: UniProtKB superoxide-generating NADPH oxidase activator activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 20	20	By similarity
	Propeptide	21 – 81	61	Removed in mature form By similarity		PRO_0000023374
	Chain	82 – 190	109	Platelet-derived growth factor subunit B		PRO_0000023375
	Propeptide	191 – 241	51	Removed in mature form By similarity		PRO_0000023376
Sites
	Site	108	1	Involved in receptor binding
	Site	111	1	Involved in receptor binding
Amino acid modifications
	Glycosylation	63	1	N-linked (GlcNAc...) Potential
	Disulfide bond	97 ↔ 141		By similarity
	Disulfide bond	124		Interchain By similarity
	Disulfide bond	130 ↔ 178		By similarity
	Disulfide bond	133		Interchain By similarity
	Disulfide bond	134 ↔ 180		By similarity
Experimental info
	Sequence conflict	183	1	I → V in AAH53430. Ref.2
	Sequence conflict	183	1	I → V in AAH64056. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P31240 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 3C5EB7A2DAD64178	FASTA	241	27,382
	10 20 30 40 50 60 MNRCWALFLP LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHR DSVDEDGAEL 70 80 90 100 110 120 DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV 130 140 150 160 170 180 WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC 190 200 210 220 230 240 ETIVTPRPVT RSPGTSREQR AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Structure of the murine c-sis proto-oncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor." Bonthron D.T., Sultan P., Collins T. Genomics 10:287-292(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J. Tissue: Embryo and Mammary gland.
[3]	"Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities." Leveen P., Pekny M., Gebre-Medhin S., Swolin B., Larsson E., Betsholtz C. Genes Dev. 8:1875-1887(1994) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[4]	"Pericyte loss and microaneurysm formation in PDGF-B-deficient mice." Lindahl P., Johansson B.R., Leveen P., Betsholtz C. Science 277:242-245(1997) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[5]	"Platelet-derived growth factor receptor beta regulates migration and DNA synthesis in metanephric mesenchymal cells." Arar M., Xu Y.C., Elshihabi I., Barnes J.L., Choudhury G.G., Abboud H.E. J. Biol. Chem. 275:9527-9533(2000) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[6]	"Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF beta-receptor null mice." Kaminski W.E., Lindahl P., Lin N.L., Broudy V.C., Crosby J.R., Hellstrom M., Swolin B., Bowen-Pope D.F., Martin P.J., Ross R., Betsholtz C., Raines E.W. Blood 97:1990-1998(2001) [PubMed] [Europe PMC] [Abstract] Cited for: DISRUPTION PHENOTYPE.
[7]	"Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury." Taneda S., Hudkins K.L., Topouzis S., Gilbertson D.G., Ophascharoensuk V., Truong L., Johnson R.J., Alpers C.E. J. Am. Soc. Nephrol. 14:2544-2555(2003) [PubMed] [Europe PMC] [Abstract] Cited for: TISSUE SPECIFICITY.
[8]	"Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors." Abramsson A., Lindblom P., Betsholtz C. J. Clin. Invest. 112:1142-1151(2003) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
[9]	"PDGF signaling in cells and mice." Tallquist M., Kazlauskas A. Cytokine Growth Factor Rev. 15:205-213(2004) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW.
[10]	"Insight into the physiological functions of PDGF through genetic studies in mice." Betsholtz C. Cytokine Growth Factor Rev. 15:215-228(2004) [PubMed] [Europe PMC] [Abstract] Cited for: REVIEW ON FUNCTION, DISRUPTION PHENOTYPE.
[11]	"Comprehensive dissection of PDGF-PDGFR signaling pathways in PDGFR genetically defined cells." Wu E., Palmer N., Tian Z., Moseman A.P., Galdzicki M., Wang X., Berger B., Zhang H., Kohane I.S. PLoS ONE 3:E3794-E3794(2008) [PubMed] [Europe PMC] [Abstract] Cited for: FUNCTION.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M84453 , M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
M64849 , M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
BC053430 mRNA. Translation: AAH53430.1.
BC064056 mRNA. Translation: AAH64056.1.

IPI

IPI00132238.

PIR

PFMSGB. A39073.

RefSeq

NP_035187.2. NM_011057.3.

UniGene

Mm.144089.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
2DZ4	model	-	A	90-185	[»]
2DZ5	model	-	A	90-185	[»]

ProteinModelPortal

P31240.

SMR

P31240. Positions 25-182.

ModBase

Search...

PTM databases

PhosphoSite

P31240.

Proteomic databases

PRIDE

P31240.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

Ensembl

ENSMUST00000000500; ENSMUSP00000000500; ENSMUSG00000000489.

GeneID

18591.

KEGG

mmu:18591.

Organism-specific databases

CTD

5155.

MGI

MGI:97528. Pdgfb.

Phylogenomic databases

eggNOG

NOG72843.

GeneTree

ENSGT00510000046755.

HOGENOM

HOG000286027.

HOVERGEN

HBG053546.

InParanoid

P31240.

KO

K04359.

OrthoDB

EOG4F4SCF.

Gene expression databases

Bgee

P31240.

CleanEx

MM_PDGFB.

Genevestigator

P31240.

GermOnline

ENSMUSG00000000489. Mus musculus.

Family and domain databases

InterPro

IPR000072. PD_growth_factor.
IPR023581. PD_growth_factor_CS.
IPR006782. PDGF_N.
[Graphical view]

Pfam

PF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]

SMART

SM00141. PDGF. 1 hit.
[Graphical view]

PROSITE

PS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

294476.

SOURCE

Search...

Entry information

Entry name

PDGFB_MOUSE

Accession

Primary (citable) accession number: P31240
Secondary accession number(s): Q6P3C4

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	May 1, 2013
This is version 110 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program