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P31240

- PDGFB_MOUSE

UniProt

P31240 - PDGFB_MOUSE

Protein

Platelet-derived growth factor subunit B

Gene

Pdgfb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 123 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.4 Publications

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei108 – 1081Involved in receptor binding
    Sitei111 – 1111Involved in receptor binding

    GO - Molecular functioni

    1. chemoattractant activity Source: Ensembl
    2. growth factor activity Source: UniProtKB
    3. platelet-derived growth factor receptor binding Source: UniProtKB
    4. protein homodimerization activity Source: UniProtKB
    5. superoxide-generating NADPH oxidase activator activity Source: UniProtKB

    GO - Biological processi

    1. actin cytoskeleton organization Source: MGI
    2. activation of protein kinase activity Source: UniProtKB
    3. activation of protein kinase B activity Source: UniProtKB
    4. blood coagulation Source: RefGenome
    5. blood vessel development Source: UniProtKB
    6. blood vessel morphogenesis Source: MGI
    7. branching involved in salivary gland morphogenesis Source: MGI
    8. cardiac vascular smooth muscle cell differentiation Source: DFLAT
    9. cell chemotaxis Source: UniProtKB
    10. cell growth Source: Ensembl
    11. cell projection assembly Source: MGI
    12. cellular response to mycophenolic acid Source: UniProtKB
    13. DNA replication Source: Ensembl
    14. embryonic placenta development Source: UniProtKB
    15. epithelial cell proliferation involved in salivary gland morphogenesis Source: MGI
    16. heart development Source: UniProtKB
    17. metanephric glomerular endothelium development Source: UniProtKB
    18. metanephric glomerular mesangial cell development Source: UniProtKB
    19. metanephric glomerular mesangial cell proliferation involved in metanephros development Source: UniProtKB
    20. monocyte chemotaxis Source: UniProtKB
    21. negative regulation of cell migration Source: MGI
    22. negative regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
    23. negative regulation of platelet activation Source: UniProtKB
    24. negative regulation of transcription, DNA-templated Source: UniProtKB
    25. negative regulation of vasodilation Source: DFLAT
    26. paracrine signaling Source: UniProtKB
    27. peptidyl-serine phosphorylation Source: UniProtKB
    28. peptidyl-tyrosine phosphorylation Source: UniProtKB
    29. platelet-derived growth factor receptor signaling pathway Source: UniProtKB
    30. positive regulation of blood vessel endothelial cell migration Source: UniProtKB
    31. positive regulation of calcium ion import Source: UniProtKB
    32. positive regulation of cell division Source: UniProtKB-KW
    33. positive regulation of cell migration Source: MGI
    34. positive regulation of cell proliferation Source: UniProtKB
    35. positive regulation of chemotaxis Source: UniProtKB
    36. positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
    37. positive regulation of DNA biosynthetic process Source: UniProtKB
    38. positive regulation of DNA replication Source: UniProtKB
    39. positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
    40. positive regulation of endothelial cell proliferation Source: MGI
    41. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
    42. positive regulation of fibroblast growth factor receptor signaling pathway Source: MGI
    43. positive regulation of fibroblast proliferation Source: UniProtKB
    44. positive regulation of glomerular filtration Source: UniProtKB
    45. positive regulation of glomerular mesangial cell proliferation Source: UniProtKB
    46. positive regulation of hyaluronan biosynthetic process Source: UniProtKB
    47. positive regulation of MAPK cascade Source: UniProtKB
    48. positive regulation of MAP kinase activity Source: UniProtKB
    49. positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway Source: UniProtKB
    50. positive regulation of mitosis Source: UniProtKB
    51. positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
    52. positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
    53. positive regulation of protein autophosphorylation Source: UniProtKB
    54. positive regulation of protein tyrosine kinase activity Source: UniProtKB
    55. positive regulation of reactive oxygen species metabolic process Source: UniProtKB
    56. positive regulation of smooth muscle cell migration Source: UniProtKB
    57. positive regulation of smooth muscle cell proliferation Source: UniProtKB
    58. positive regulation of transcription, DNA-templated Source: UniProtKB
    59. positive regulation of vasoconstriction Source: DFLAT
    60. protein phosphorylation Source: UniProtKB
    61. reactive oxygen species metabolic process Source: UniProtKB
    62. regulation of peptidyl-tyrosine phosphorylation Source: MGI
    63. response to drug Source: Ensembl
    64. response to estradiol Source: Ensembl
    65. response to hypoxia Source: Ensembl
    66. response to insulin Source: Ensembl
    67. response to wounding Source: UniProtKB
    68. substrate-dependent cell migration Source: MGI
    69. transforming growth factor beta receptor signaling pathway Source: UniProtKB

    Keywords - Molecular functioni

    Developmental protein, Growth factor, Mitogen

    Enzyme and pathway databases

    ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_196607. Non-integrin membrane-ECM interactions.
    REACT_226341. PIP3 activates AKT signaling.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Platelet-derived growth factor subunit B
    Short name:
    PDGF subunit B
    Alternative name(s):
    PDGF-2
    Platelet-derived growth factor B chain
    Platelet-derived growth factor beta polypeptide
    Proto-oncogene c-Sis
    Gene namesi
    Name:Pdgfb
    Synonyms:Sis
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 15

    Organism-specific databases

    MGIiMGI:97528. Pdgfb.

    Subcellular locationi

    Secreted
    Note: Released by platelets upon wounding.By similarity

    GO - Cellular componenti

    1. basolateral plasma membrane Source: UniProtKB
    2. cell surface Source: UniProtKB
    3. cytoplasm Source: UniProtKB
    4. extracellular space Source: RefGenome

    Keywords - Cellular componenti

    Secreted

    Pathology & Biotechi

    Disruption phenotypei

    Perinatal lethality, due to severe hemorrhages shortly before birth. Kidney glomerular tufts do not form, apparently because of absence of mesangial cells. The heart and some large arteries are dilated in late-stage embryos. Mice lack microvascular pericytes, which normally form part of the capillary wall, and develop numerous capillary microaneurysms, leading to hemorrhages. Mice display erythroblastosis, macrocytic anemia, and thrombocytopenia.4 Publications

    Keywords - Diseasei

    Proto-oncogene

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2020By similarityAdd
    BLAST
    Propeptidei21 – 8161Removed in mature formBy similarityPRO_0000023374Add
    BLAST
    Chaini82 – 190109Platelet-derived growth factor subunit BPRO_0000023375Add
    BLAST
    Propeptidei191 – 24151Removed in mature formBy similarityPRO_0000023376Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
    Disulfide bondi97 ↔ 141By similarity
    Disulfide bondi124 – 124InterchainBy similarity
    Disulfide bondi130 ↔ 178By similarity
    Disulfide bondi133 – 133InterchainBy similarity
    Disulfide bondi134 ↔ 180By similarity

    Keywords - PTMi

    Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

    Proteomic databases

    PRIDEiP31240.

    PTM databases

    PhosphoSiteiP31240.

    Expressioni

    Tissue specificityi

    Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4.1 Publication

    Gene expression databases

    BgeeiP31240.
    CleanExiMM_PDGFB.
    GenevestigatoriP31240.

    Interactioni

    Subunit structurei

    Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 By similarity.By similarity

    Structurei

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    2DZ4model-A90-185[»]
    2DZ5model-A90-185[»]
    ProteinModelPortaliP31240.
    SMRiP31240. Positions 25-182.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the PDGF/VEGF growth factor family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG72843.
    GeneTreeiENSGT00510000046755.
    HOGENOMiHOG000286027.
    HOVERGENiHBG053546.
    InParanoidiP31240.
    KOiK17386.
    OrthoDBiEOG78H3VV.
    PhylomeDBiP31240.
    TreeFamiTF319554.

    Family and domain databases

    Gene3Di2.10.90.10. 1 hit.
    InterProiIPR029034. Cystine-knot_cytokine.
    IPR023581. PD_growth_factor_CS.
    IPR000072. PDGF/VEGF_dom.
    IPR006782. PDGF_N.
    [Graphical view]
    PfamiPF00341. PDGF. 1 hit.
    PF04692. PDGF_N. 1 hit.
    [Graphical view]
    SMARTiSM00141. PDGF. 1 hit.
    [Graphical view]
    SUPFAMiSSF57501. SSF57501. 1 hit.
    PROSITEiPS00249. PDGF_1. 1 hit.
    PS50278. PDGF_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P31240-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRCWALFLP LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHR    50
    DSVDEDGAEL DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR 100
    TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP 150
    VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT RSPGTSREQR 200
    AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A 241
    Length:241
    Mass (Da):27,382
    Last modified:July 1, 1993 - v1
    Checksum:i3C5EB7A2DAD64178
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti183 – 1831I → V in AAH53430. (PubMed:15489334)Curated
    Sequence conflicti183 – 1831I → V in AAH64056. (PubMed:15489334)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84453
    , M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
    M64849
    , M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
    BC053430 mRNA. Translation: AAH53430.1.
    BC064056 mRNA. Translation: AAH64056.1.
    CCDSiCCDS27656.1.
    PIRiA39073. PFMSGB.
    RefSeqiNP_035187.2. NM_011057.3.
    UniGeneiMm.144089.

    Genome annotation databases

    EnsembliENSMUST00000000500; ENSMUSP00000000500; ENSMUSG00000000489.
    GeneIDi18591.
    KEGGimmu:18591.
    UCSCiuc007wuz.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M84453
    , M84448 , M84449 , M84450 , M84451 , M84452 Genomic DNA. Translation: AAA40113.1 .
    M64849
    , M64844 , M64845 , M64846 , M64847 , M64848 Genomic DNA. Translation: AAA37485.1 .
    BC053430 mRNA. Translation: AAH53430.1 .
    BC064056 mRNA. Translation: AAH64056.1 .
    CCDSi CCDS27656.1.
    PIRi A39073. PFMSGB.
    RefSeqi NP_035187.2. NM_011057.3.
    UniGenei Mm.144089.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    2DZ4 model - A 90-185 [» ]
    2DZ5 model - A 90-185 [» ]
    ProteinModelPortali P31240.
    SMRi P31240. Positions 25-182.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei P31240.

    Proteomic databases

    PRIDEi P31240.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000000500 ; ENSMUSP00000000500 ; ENSMUSG00000000489 .
    GeneIDi 18591.
    KEGGi mmu:18591.
    UCSCi uc007wuz.1. mouse.

    Organism-specific databases

    CTDi 5155.
    MGIi MGI:97528. Pdgfb.

    Phylogenomic databases

    eggNOGi NOG72843.
    GeneTreei ENSGT00510000046755.
    HOGENOMi HOG000286027.
    HOVERGENi HBG053546.
    InParanoidi P31240.
    KOi K17386.
    OrthoDBi EOG78H3VV.
    PhylomeDBi P31240.
    TreeFami TF319554.

    Enzyme and pathway databases

    Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
    REACT_196607. Non-integrin membrane-ECM interactions.
    REACT_226341. PIP3 activates AKT signaling.

    Miscellaneous databases

    NextBioi 294476.
    PROi P31240.
    SOURCEi Search...

    Gene expression databases

    Bgeei P31240.
    CleanExi MM_PDGFB.
    Genevestigatori P31240.

    Family and domain databases

    Gene3Di 2.10.90.10. 1 hit.
    InterProi IPR029034. Cystine-knot_cytokine.
    IPR023581. PD_growth_factor_CS.
    IPR000072. PDGF/VEGF_dom.
    IPR006782. PDGF_N.
    [Graphical view ]
    Pfami PF00341. PDGF. 1 hit.
    PF04692. PDGF_N. 1 hit.
    [Graphical view ]
    SMARTi SM00141. PDGF. 1 hit.
    [Graphical view ]
    SUPFAMi SSF57501. SSF57501. 1 hit.
    PROSITEi PS00249. PDGF_1. 1 hit.
    PS50278. PDGF_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Structure of the murine c-sis proto-oncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor."
      Bonthron D.T., Sultan P., Collins T.
      Genomics 10:287-292(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: C57BL/6J.
      Tissue: Embryo and Mammary gland.
    3. "Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities."
      Leveen P., Pekny M., Gebre-Medhin S., Swolin B., Larsson E., Betsholtz C.
      Genes Dev. 8:1875-1887(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE, FUNCTION.
    4. "Pericyte loss and microaneurysm formation in PDGF-B-deficient mice."
      Lindahl P., Johansson B.R., Leveen P., Betsholtz C.
      Science 277:242-245(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    5. "Platelet-derived growth factor receptor beta regulates migration and DNA synthesis in metanephric mesenchymal cells."
      Arar M., Xu Y.C., Elshihabi I., Barnes J.L., Choudhury G.G., Abboud H.E.
      J. Biol. Chem. 275:9527-9533(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    6. "Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF beta-receptor null mice."
      Kaminski W.E., Lindahl P., Lin N.L., Broudy V.C., Crosby J.R., Hellstrom M., Swolin B., Bowen-Pope D.F., Martin P.J., Ross R., Betsholtz C., Raines E.W.
      Blood 97:1990-1998(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: DISRUPTION PHENOTYPE.
    7. "Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury."
      Taneda S., Hudkins K.L., Topouzis S., Gilbertson D.G., Ophascharoensuk V., Truong L., Johnson R.J., Alpers C.E.
      J. Am. Soc. Nephrol. 14:2544-2555(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.
    8. "Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors."
      Abramsson A., Lindblom P., Betsholtz C.
      J. Clin. Invest. 112:1142-1151(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.
    9. Cited for: REVIEW.
    10. "Insight into the physiological functions of PDGF through genetic studies in mice."
      Betsholtz C.
      Cytokine Growth Factor Rev. 15:215-228(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: REVIEW ON FUNCTION, DISRUPTION PHENOTYPE.
    11. "Comprehensive dissection of PDGF-PDGFR signaling pathways in PDGFR genetically defined cells."
      Wu E., Palmer N., Tian Z., Moseman A.P., Galdzicki M., Wang X., Berger B., Zhang H., Kohane I.S.
      PLoS ONE 3:E3794-E3794(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION.

    Entry informationi

    Entry nameiPDGFB_MOUSE
    AccessioniPrimary (citable) accession number: P31240
    Secondary accession number(s): Q6P3C4
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 123 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3