Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P31240

- PDGFB_MOUSE

UniProt

P31240 - PDGFB_MOUSE

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Platelet-derived growth factor subunit B

Gene

Pdgfb

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli

Functioni

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.4 Publications

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei108 – 1081Involved in receptor binding
Sitei111 – 1111Involved in receptor binding

GO - Molecular functioni

  1. chemoattractant activity Source: Ensembl
  2. growth factor activity Source: UniProtKB
  3. platelet-derived growth factor receptor binding Source: UniProtKB
  4. protein homodimerization activity Source: UniProtKB
  5. superoxide-generating NADPH oxidase activator activity Source: UniProtKB

GO - Biological processi

  1. actin cytoskeleton organization Source: MGI
  2. activation of protein kinase activity Source: UniProtKB
  3. activation of protein kinase B activity Source: UniProtKB
  4. blood coagulation Source: RefGenome
  5. blood vessel development Source: UniProtKB
  6. blood vessel morphogenesis Source: MGI
  7. branching involved in salivary gland morphogenesis Source: MGI
  8. cardiac vascular smooth muscle cell differentiation Source: DFLAT
  9. cell chemotaxis Source: UniProtKB
  10. cell growth Source: Ensembl
  11. cell projection assembly Source: MGI
  12. cellular response to mycophenolic acid Source: UniProtKB
  13. DNA replication Source: Ensembl
  14. embryonic placenta development Source: UniProtKB
  15. epithelial cell proliferation involved in salivary gland morphogenesis Source: MGI
  16. heart development Source: UniProtKB
  17. metanephric glomerular endothelium development Source: UniProtKB
  18. metanephric glomerular mesangial cell development Source: UniProtKB
  19. metanephric glomerular mesangial cell proliferation involved in metanephros development Source: UniProtKB
  20. monocyte chemotaxis Source: UniProtKB
  21. negative regulation of cell migration Source: MGI
  22. negative regulation of phosphatidylinositol biosynthetic process Source: UniProtKB
  23. negative regulation of platelet activation Source: UniProtKB
  24. negative regulation of transcription, DNA-templated Source: UniProtKB
  25. negative regulation of vasodilation Source: DFLAT
  26. paracrine signaling Source: UniProtKB
  27. peptidyl-serine phosphorylation Source: UniProtKB
  28. peptidyl-tyrosine phosphorylation Source: UniProtKB
  29. platelet-derived growth factor receptor signaling pathway Source: UniProtKB
  30. positive regulation of blood vessel endothelial cell migration Source: UniProtKB
  31. positive regulation of calcium ion import Source: UniProtKB
  32. positive regulation of cell division Source: UniProtKB-KW
  33. positive regulation of cell migration Source: MGI
  34. positive regulation of cell proliferation Source: UniProtKB
  35. positive regulation of chemotaxis Source: UniProtKB
  36. positive regulation of cyclin-dependent protein serine/threonine kinase activity Source: UniProtKB
  37. positive regulation of DNA biosynthetic process Source: UniProtKB
  38. positive regulation of DNA replication Source: UniProtKB
  39. positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway Source: BHF-UCL
  40. positive regulation of endothelial cell proliferation Source: MGI
  41. positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
  42. positive regulation of fibroblast growth factor receptor signaling pathway Source: MGI
  43. positive regulation of fibroblast proliferation Source: UniProtKB
  44. positive regulation of glomerular filtration Source: UniProtKB
  45. positive regulation of glomerular mesangial cell proliferation Source: UniProtKB
  46. positive regulation of hyaluronan biosynthetic process Source: UniProtKB
  47. positive regulation of MAPK cascade Source: UniProtKB
  48. positive regulation of MAP kinase activity Source: UniProtKB
  49. positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway Source: UniProtKB
  50. positive regulation of mitosis Source: UniProtKB
  51. positive regulation of mitotic cell cycle, embryonic Source: MGI
  52. positive regulation of phosphatidylinositol 3-kinase activity Source: UniProtKB
  53. positive regulation of phosphatidylinositol 3-kinase signaling Source: UniProtKB
  54. positive regulation of protein autophosphorylation Source: UniProtKB
  55. positive regulation of protein tyrosine kinase activity Source: UniProtKB
  56. positive regulation of reactive oxygen species metabolic process Source: UniProtKB
  57. positive regulation of smooth muscle cell migration Source: UniProtKB
  58. positive regulation of smooth muscle cell proliferation Source: UniProtKB
  59. positive regulation of transcription, DNA-templated Source: UniProtKB
  60. positive regulation of vasoconstriction Source: DFLAT
  61. protein phosphorylation Source: UniProtKB
  62. reactive oxygen species metabolic process Source: UniProtKB
  63. regulation of peptidyl-tyrosine phosphorylation Source: MGI
  64. response to drug Source: Ensembl
  65. response to estradiol Source: Ensembl
  66. response to hypoxia Source: Ensembl
  67. response to insulin Source: Ensembl
  68. response to wounding Source: UniProtKB
  69. substrate-dependent cell migration Source: MGI
  70. transforming growth factor beta receptor signaling pathway Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Enzyme and pathway databases

ReactomeiREACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_196607. Non-integrin membrane-ECM interactions.
REACT_226341. PIP3 activates AKT signaling.

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-derived growth factor subunit B
Short name:
PDGF subunit B
Alternative name(s):
PDGF-2
Platelet-derived growth factor B chain
Platelet-derived growth factor beta polypeptide
Proto-oncogene c-Sis
Gene namesi
Name:Pdgfb
Synonyms:Sis
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 15

Organism-specific databases

MGIiMGI:97528. Pdgfb.

Subcellular locationi

Secreted
Note: Released by platelets upon wounding.By similarity

GO - Cellular componenti

  1. basolateral plasma membrane Source: UniProtKB
  2. cell surface Source: UniProtKB
  3. cytoplasm Source: UniProtKB
  4. extracellular space Source: RefGenome
Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Perinatal lethality, due to severe hemorrhages shortly before birth. Kidney glomerular tufts do not form, apparently because of absence of mesangial cells. The heart and some large arteries are dilated in late-stage embryos. Mice lack microvascular pericytes, which normally form part of the capillary wall, and develop numerous capillary microaneurysms, leading to hemorrhages. Mice display erythroblastosis, macrocytic anemia, and thrombocytopenia.4 Publications

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2020By similarityAdd
BLAST
Propeptidei21 – 8161Removed in mature formBy similarityPRO_0000023374Add
BLAST
Chaini82 – 190109Platelet-derived growth factor subunit BPRO_0000023375Add
BLAST
Propeptidei191 – 24151Removed in mature formBy similarityPRO_0000023376Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi63 – 631N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi97 ↔ 141By similarity
Disulfide bondi124 – 124InterchainBy similarity
Disulfide bondi130 ↔ 178By similarity
Disulfide bondi133 – 133InterchainBy similarity
Disulfide bondi134 ↔ 180By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

PRIDEiP31240.

PTM databases

PhosphoSiteiP31240.

Expressioni

Tissue specificityi

Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4.1 Publication

Gene expression databases

BgeeiP31240.
CleanExiMM_PDGFB.
GenevestigatoriP31240.

Interactioni

Subunit structurei

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 By similarity.By similarity

Structurei

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
2DZ4model-A90-185[»]
2DZ5model-A90-185[»]
ProteinModelPortaliP31240.
SMRiP31240. Positions 25-182.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG72843.
GeneTreeiENSGT00510000046755.
HOGENOMiHOG000286027.
HOVERGENiHBG053546.
InParanoidiP31240.
KOiK17386.
OrthoDBiEOG78H3VV.
PhylomeDBiP31240.
TreeFamiTF319554.

Family and domain databases

Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31240-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRCWALFLP LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHR
60 70 80 90 100
DSVDEDGAEL DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR
110 120 130 140 150
TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP
160 170 180 190 200
VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT RSPGTSREQR
210 220 230 240
AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A
Length:241
Mass (Da):27,382
Last modified:July 1, 1993 - v1
Checksum:i3C5EB7A2DAD64178
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti183 – 1831I → V in AAH53430. (PubMed:15489334)Curated
Sequence conflicti183 – 1831I → V in AAH64056. (PubMed:15489334)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84453
, M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
M64849
, M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
BC053430 mRNA. Translation: AAH53430.1.
BC064056 mRNA. Translation: AAH64056.1.
CCDSiCCDS27656.1.
PIRiA39073. PFMSGB.
RefSeqiNP_035187.2. NM_011057.3.
UniGeneiMm.144089.

Genome annotation databases

EnsembliENSMUST00000000500; ENSMUSP00000000500; ENSMUSG00000000489.
GeneIDi18591.
KEGGimmu:18591.
UCSCiuc007wuz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M84453
, M84448 , M84449 , M84450 , M84451 , M84452 Genomic DNA. Translation: AAA40113.1 .
M64849
, M64844 , M64845 , M64846 , M64847 , M64848 Genomic DNA. Translation: AAA37485.1 .
BC053430 mRNA. Translation: AAH53430.1 .
BC064056 mRNA. Translation: AAH64056.1 .
CCDSi CCDS27656.1.
PIRi A39073. PFMSGB.
RefSeqi NP_035187.2. NM_011057.3.
UniGenei Mm.144089.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
2DZ4 model - A 90-185 [» ]
2DZ5 model - A 90-185 [» ]
ProteinModelPortali P31240.
SMRi P31240. Positions 25-182.
ModBasei Search...
MobiDBi Search...

PTM databases

PhosphoSitei P31240.

Proteomic databases

PRIDEi P31240.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000000500 ; ENSMUSP00000000500 ; ENSMUSG00000000489 .
GeneIDi 18591.
KEGGi mmu:18591.
UCSCi uc007wuz.1. mouse.

Organism-specific databases

CTDi 5155.
MGIi MGI:97528. Pdgfb.

Phylogenomic databases

eggNOGi NOG72843.
GeneTreei ENSGT00510000046755.
HOGENOMi HOG000286027.
HOVERGENi HBG053546.
InParanoidi P31240.
KOi K17386.
OrthoDBi EOG78H3VV.
PhylomeDBi P31240.
TreeFami TF319554.

Enzyme and pathway databases

Reactomei REACT_196588. Constitutive PI3K/AKT Signaling in Cancer.
REACT_196607. Non-integrin membrane-ECM interactions.
REACT_226341. PIP3 activates AKT signaling.

Miscellaneous databases

NextBioi 294476.
PROi P31240.
SOURCEi Search...

Gene expression databases

Bgeei P31240.
CleanExi MM_PDGFB.
Genevestigatori P31240.

Family and domain databases

Gene3Di 2.10.90.10. 1 hit.
InterProi IPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view ]
Pfami PF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view ]
SMARTi SM00141. PDGF. 1 hit.
[Graphical view ]
SUPFAMi SSF57501. SSF57501. 1 hit.
PROSITEi PS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Structure of the murine c-sis proto-oncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor."
    Bonthron D.T., Sultan P., Collins T.
    Genomics 10:287-292(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J.
    Tissue: Embryo and Mammary gland.
  3. "Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities."
    Leveen P., Pekny M., Gebre-Medhin S., Swolin B., Larsson E., Betsholtz C.
    Genes Dev. 8:1875-1887(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE, FUNCTION.
  4. "Pericyte loss and microaneurysm formation in PDGF-B-deficient mice."
    Lindahl P., Johansson B.R., Leveen P., Betsholtz C.
    Science 277:242-245(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  5. "Platelet-derived growth factor receptor beta regulates migration and DNA synthesis in metanephric mesenchymal cells."
    Arar M., Xu Y.C., Elshihabi I., Barnes J.L., Choudhury G.G., Abboud H.E.
    J. Biol. Chem. 275:9527-9533(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  6. "Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF beta-receptor null mice."
    Kaminski W.E., Lindahl P., Lin N.L., Broudy V.C., Crosby J.R., Hellstrom M., Swolin B., Bowen-Pope D.F., Martin P.J., Ross R., Betsholtz C., Raines E.W.
    Blood 97:1990-1998(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: DISRUPTION PHENOTYPE.
  7. "Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury."
    Taneda S., Hudkins K.L., Topouzis S., Gilbertson D.G., Ophascharoensuk V., Truong L., Johnson R.J., Alpers C.E.
    J. Am. Soc. Nephrol. 14:2544-2555(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: TISSUE SPECIFICITY.
  8. "Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors."
    Abramsson A., Lindblom P., Betsholtz C.
    J. Clin. Invest. 112:1142-1151(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.
  9. Cited for: REVIEW.
  10. "Insight into the physiological functions of PDGF through genetic studies in mice."
    Betsholtz C.
    Cytokine Growth Factor Rev. 15:215-228(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: REVIEW ON FUNCTION, DISRUPTION PHENOTYPE.
  11. "Comprehensive dissection of PDGF-PDGFR signaling pathways in PDGFR genetically defined cells."
    Wu E., Palmer N., Tian Z., Moseman A.P., Galdzicki M., Wang X., Berger B., Zhang H., Kohane I.S.
    PLoS ONE 3:E3794-E3794(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION.

Entry informationi

Entry nameiPDGFB_MOUSE
AccessioniPrimary (citable) accession number: P31240
Secondary accession number(s): Q6P3C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: October 29, 2014
This is version 124 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3