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P31240 (PDGFB_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 121. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Platelet-derived growth factor subunit B

Short name=PDGF subunit B
Alternative name(s):
PDGF-2
Platelet-derived growth factor B chain
Platelet-derived growth factor beta polypeptide
Proto-oncogene c-Sis
Gene names
Name:Pdgfb
Synonyms:Sis
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length241 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA. Ref.3 Ref.5 Ref.8 Ref.11

Subunit structure

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 By similarity.

Subcellular location

Secreted. Note: Released by platelets upon wounding By similarity.

Tissue specificity

Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4. Ref.7

Disruption phenotype

Perinatal lethality, due to severe hemorrhages shortly before birth. Kidney glomerular tufts do not form, apparently because of absence of mesangial cells. The heart and some large arteries are dilated in late-stage embryos. Mice lack microvascular pericytes, which normally form part of the capillary wall, and develop numerous capillary microaneurysms, leading to hemorrhages. Mice display erythroblastosis, macrocytic anemia, and thrombocytopenia. Ref.3 Ref.4 Ref.6 Ref.10

Sequence similarities

Belongs to the PDGF/VEGF growth factor family.

Ontologies

Keywords
   Cellular componentSecreted
   DiseaseProto-oncogene
   DomainSignal
   Molecular functionDevelopmental protein
Growth factor
Mitogen
   PTMCleavage on pair of basic residues
Disulfide bond
Glycoprotein
   Technical term3D-structure
Complete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA replication

Inferred from electronic annotation. Source: Ensembl

actin cytoskeleton organization

Inferred from direct assay PubMed 12176888. Source: MGI

activation of protein kinase B activity

Inferred from sequence or structural similarity. Source: UniProtKB

activation of protein kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

blood coagulation

Inferred from Biological aspect of Ancestor. Source: RefGenome

blood vessel development

Inferred from expression pattern PubMed 10375497. Source: UniProtKB

blood vessel morphogenesis

Inferred from direct assay PubMed 15919073. Source: MGI

branching involved in salivary gland morphogenesis

Inferred from mutant phenotype PubMed 18559345. Source: MGI

cardiac vascular smooth muscle cell differentiation

Traceable author statement PubMed 20299672. Source: DFLAT

cell chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

cell growth

Inferred from electronic annotation. Source: Ensembl

cell projection assembly

Inferred from direct assay PubMed 12176888. Source: MGI

cellular response to mycophenolic acid

Inferred from sequence or structural similarity. Source: UniProtKB

embryonic placenta development

Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB

epithelial cell proliferation involved in salivary gland morphogenesis

Inferred from mutant phenotype PubMed 18559345. Source: MGI

heart development

Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB

metanephric glomerular endothelium development

Inferred from expression pattern PubMed 9693135. Source: UniProtKB

metanephric glomerular mesangial cell development

Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB

metanephric glomerular mesangial cell proliferation involved in metanephros development

Inferred from mutant phenotype PubMed 9693135. Source: UniProtKB

monocyte chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of cell migration

Inferred from genetic interaction PubMed 16644864. Source: MGI

negative regulation of phosphatidylinositol biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of platelet activation

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

negative regulation of vasodilation

Traceable author statement PubMed 20299672. Source: DFLAT

paracrine signaling

Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB

peptidyl-serine phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

peptidyl-tyrosine phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

platelet-derived growth factor receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of DNA biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of DNA replication

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of ERK1 and ERK2 cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of MAP kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of MAPK cascade

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of blood vessel endothelial cell migration

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of calcium ion import

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cell division

Inferred from electronic annotation. Source: UniProtKB-KW

positive regulation of cell migration

Inferred from genetic interaction PubMed 16456542. Source: MGI

positive regulation of cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of chemotaxis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of cyclin-dependent protein kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of endothelial cell chemotaxis by VEGF-activated vascular endothelial growth factor receptor signaling pathway

Traceable author statement PubMed 18663143. Source: BHF-UCL

positive regulation of endothelial cell proliferation

Inferred from genetic interaction PubMed 16644864. Source: MGI

positive regulation of fibroblast growth factor receptor signaling pathway

Inferred from direct assay PubMed 18559345. Source: MGI

positive regulation of fibroblast proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of glomerular filtration

Inferred from mutant phenotype PubMed 15084468. Source: UniProtKB

positive regulation of glomerular mesangial cell proliferation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of hyaluronan biosynthetic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of metanephric mesenchymal cell migration by platelet-derived growth factor receptor-beta signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of mitosis

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of phosphatidylinositol 3-kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of phosphatidylinositol 3-kinase signaling

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of protein autophosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of protein tyrosine kinase activity

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of reactive oxygen species metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of smooth muscle cell migration

Inferred from mutant phenotype PubMed 10375497. Source: UniProtKB

positive regulation of smooth muscle cell proliferation

Inferred from mutant phenotype PubMed 10375497. Source: UniProtKB

positive regulation of transcription, DNA-templated

Inferred from sequence or structural similarity. Source: UniProtKB

positive regulation of vasoconstriction

Traceable author statement PubMed 20299672. Source: DFLAT

protein phosphorylation

Inferred from sequence or structural similarity. Source: UniProtKB

reactive oxygen species metabolic process

Inferred from sequence or structural similarity. Source: UniProtKB

regulation of peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 11940581. Source: MGI

response to drug

Inferred from electronic annotation. Source: Ensembl

response to estradiol

Inferred from electronic annotation. Source: Ensembl

response to hypoxia

Inferred from electronic annotation. Source: Ensembl

response to insulin

Inferred from electronic annotation. Source: Ensembl

response to wounding

Inferred from sequence or structural similarity. Source: UniProtKB

substrate-dependent cell migration

Inferred from direct assay PubMed 15183724. Source: MGI

transforming growth factor beta receptor signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

   Cellular_componentbasolateral plasma membrane

Inferred from sequence or structural similarity. Source: UniProtKB

cell surface

Inferred from sequence or structural similarity. Source: UniProtKB

cytoplasm

Inferred from sequence or structural similarity. Source: UniProtKB

extracellular space

Inferred from Biological aspect of Ancestor. Source: RefGenome

   Molecular_functionchemoattractant activity

Inferred from electronic annotation. Source: Ensembl

growth factor activity

Inferred from sequence or structural similarity. Source: UniProtKB

platelet-derived growth factor receptor binding

Inferred from sequence or structural similarity. Source: UniProtKB

protein homodimerization activity

Inferred from sequence or structural similarity. Source: UniProtKB

superoxide-generating NADPH oxidase activator activity

Inferred from sequence or structural similarity. Source: UniProtKB

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2020 By similarity
Propeptide21 – 8161Removed in mature form By similarity
PRO_0000023374
Chain82 – 190109Platelet-derived growth factor subunit B
PRO_0000023375
Propeptide191 – 24151Removed in mature form By similarity
PRO_0000023376

Sites

Site1081Involved in receptor binding
Site1111Involved in receptor binding

Amino acid modifications

Glycosylation631N-linked (GlcNAc...) Potential
Disulfide bond97 ↔ 141 By similarity
Disulfide bond124Interchain By similarity
Disulfide bond130 ↔ 178 By similarity
Disulfide bond133Interchain By similarity
Disulfide bond134 ↔ 180 By similarity

Experimental info

Sequence conflict1831I → V in AAH53430. Ref.2
Sequence conflict1831I → V in AAH64056. Ref.2

Sequences

Sequence LengthMass (Da)Tools
P31240 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: 3C5EB7A2DAD64178

FASTA24127,382
        10         20         30         40         50         60 
MNRCWALFLP LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHR DSVDEDGAEL 

        70         80         90        100        110        120 
DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR TEVFQISRNL IDRTNANFLV 

       130        140        150        160        170        180 
WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP VQVRKIEIVR KKPIFKKATV TLEDHLACKC 

       190        200        210        220        230        240 
ETIVTPRPVT RSPGTSREQR AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG 


A 

« Hide

References

« Hide 'large scale' references
[1]"Structure of the murine c-sis proto-oncogene (Sis, PDGFB) encoding the B chain of platelet-derived growth factor."
Bonthron D.T., Sultan P., Collins T.
Genomics 10:287-292(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: C57BL/6J.
Tissue: Embryo and Mammary gland.
[3]"Mice deficient for PDGF B show renal, cardiovascular, and hematological abnormalities."
Leveen P., Pekny M., Gebre-Medhin S., Swolin B., Larsson E., Betsholtz C.
Genes Dev. 8:1875-1887(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE, FUNCTION.
[4]"Pericyte loss and microaneurysm formation in PDGF-B-deficient mice."
Lindahl P., Johansson B.R., Leveen P., Betsholtz C.
Science 277:242-245(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[5]"Platelet-derived growth factor receptor beta regulates migration and DNA synthesis in metanephric mesenchymal cells."
Arar M., Xu Y.C., Elshihabi I., Barnes J.L., Choudhury G.G., Abboud H.E.
J. Biol. Chem. 275:9527-9533(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[6]"Basis of hematopoietic defects in platelet-derived growth factor (PDGF)-B and PDGF beta-receptor null mice."
Kaminski W.E., Lindahl P., Lin N.L., Broudy V.C., Crosby J.R., Hellstrom M., Swolin B., Bowen-Pope D.F., Martin P.J., Ross R., Betsholtz C., Raines E.W.
Blood 97:1990-1998(2001) [PubMed] [Europe PMC] [Abstract]
Cited for: DISRUPTION PHENOTYPE.
[7]"Obstructive uropathy in mice and humans: potential role for PDGF-D in the progression of tubulointerstitial injury."
Taneda S., Hudkins K.L., Topouzis S., Gilbertson D.G., Ophascharoensuk V., Truong L., Johnson R.J., Alpers C.E.
J. Am. Soc. Nephrol. 14:2544-2555(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: TISSUE SPECIFICITY.
[8]"Endothelial and nonendothelial sources of PDGF-B regulate pericyte recruitment and influence vascular pattern formation in tumors."
Abramsson A., Lindblom P., Betsholtz C.
J. Clin. Invest. 112:1142-1151(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
[9]"PDGF signaling in cells and mice."
Tallquist M., Kazlauskas A.
Cytokine Growth Factor Rev. 15:205-213(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW.
[10]"Insight into the physiological functions of PDGF through genetic studies in mice."
Betsholtz C.
Cytokine Growth Factor Rev. 15:215-228(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: REVIEW ON FUNCTION, DISRUPTION PHENOTYPE.
[11]"Comprehensive dissection of PDGF-PDGFR signaling pathways in PDGFR genetically defined cells."
Wu E., Palmer N., Tian Z., Moseman A.P., Galdzicki M., Wang X., Berger B., Zhang H., Kohane I.S.
PLoS ONE 3:E3794-E3794(2008) [PubMed] [Europe PMC] [Abstract]
Cited for: FUNCTION.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M84453 expand/collapse EMBL AC list , M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
M64849 expand/collapse EMBL AC list , M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
BC053430 mRNA. Translation: AAH53430.1.
BC064056 mRNA. Translation: AAH64056.1.
CCDSCCDS27656.1.
PIRPFMSGB. A39073.
RefSeqNP_035187.2. NM_011057.3.
UniGeneMm.144089.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2DZ4model-A90-185[»]
2DZ5model-A90-185[»]
ProteinModelPortalP31240.
SMRP31240. Positions 25-182.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteP31240.

Proteomic databases

PRIDEP31240.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000000500; ENSMUSP00000000500; ENSMUSG00000000489.
GeneID18591.
KEGGmmu:18591.
UCSCuc007wuz.1. mouse.

Organism-specific databases

CTD5155.
MGIMGI:97528. Pdgfb.

Phylogenomic databases

eggNOGNOG72843.
GeneTreeENSGT00510000046755.
HOGENOMHOG000286027.
HOVERGENHBG053546.
InParanoidP31240.
KOK17386.
OrthoDBEOG78H3VV.
PhylomeDBP31240.
TreeFamTF319554.

Gene expression databases

BgeeP31240.
CleanExMM_PDGFB.
GenevestigatorP31240.

Family and domain databases

Gene3D2.10.90.10. 1 hit.
InterProIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMSSF57501. SSF57501. 1 hit.
PROSITEPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio294476.
PROP31240.
SOURCESearch...

Entry information

Entry namePDGFB_MOUSE
AccessionPrimary (citable) accession number: P31240
Secondary accession number(s): Q6P3C4
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: July 9, 2014
This is version 121 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot