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Protein

Platelet-derived growth factor subunit B

Gene

Pdgfb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at transcript leveli

Functioni

Growth factor that plays an essential role in the regulation of embryonic development, cell proliferation, cell migration, survival and chemotaxis. Potent mitogen for cells of mesenchymal origin. Required for normal proliferation and recruitment of pericytes and vascular smooth muscle cells in the central nervous system, skin, lung, heart and placenta. Required for normal blood vessel development, and for normal development of kidney glomeruli. Plays an important role in wound healing. Signaling is modulated by the formation of heterodimers with PDGFA.4 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei108Involved in receptor binding1
Sitei111Involved in receptor binding1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Developmental protein, Growth factor, Mitogen

Names & Taxonomyi

Protein namesi
Recommended name:
Platelet-derived growth factor subunit B
Short name:
PDGF subunit B
Alternative name(s):
PDGF-2
Platelet-derived growth factor B chain
Platelet-derived growth factor beta polypeptide
Proto-oncogene c-Sis
Gene namesi
Name:Pdgfb
Synonyms:Sis
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:97528. Pdgfb.

Subcellular locationi

  • Secreted

  • Note: Released by platelets upon wounding.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Perinatal lethality, due to severe hemorrhages shortly before birth. Kidney glomerular tufts do not form, apparently because of absence of mesangial cells. The heart and some large arteries are dilated in late-stage embryos. Mice lack microvascular pericytes, which normally form part of the capillary wall, and develop numerous capillary microaneurysms, leading to hemorrhages. Mice display erythroblastosis, macrocytic anemia, and thrombocytopenia.4 Publications

Keywords - Diseasei

Proto-oncogene

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 20By similarityAdd BLAST20
PropeptideiPRO_000002337421 – 81Removed in mature formBy similarityAdd BLAST61
ChainiPRO_000002337582 – 190Platelet-derived growth factor subunit BAdd BLAST109
PropeptideiPRO_0000023376191 – 241Removed in mature formBy similarityAdd BLAST51

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi63N-linked (GlcNAc...)Sequence analysis1
Disulfide bondi97 ↔ 141By similarity
Disulfide bondi124InterchainBy similarity
Disulfide bondi130 ↔ 178By similarity
Disulfide bondi133InterchainBy similarity
Disulfide bondi134 ↔ 180By similarity

Keywords - PTMi

Cleavage on pair of basic residues, Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP31240.
PaxDbiP31240.
PeptideAtlasiP31240.
PRIDEiP31240.

PTM databases

iPTMnetiP31240.
PhosphoSitePlusiP31240.

Expressioni

Tissue specificityi

Localized to vascular smooth muscle cells. Also weakly expressed by cortical interstitial cells but absent in tubules. Up-regulated in areas of renal fibrosis. In mice with unilateral ureteral obstruction, an increased expression in interstitial cells and in some tubules observed after day 4.1 Publication

Gene expression databases

BgeeiENSMUSG00000000489.
CleanExiMM_PDGFB.

Interactioni

Subunit structurei

Homodimer; antiparallel disulfide-linked dimer. Heterodimer with PDGFA; antiparallel disulfide-linked dimer. The PDGFB homodimer interacts with PDGFRA and PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. The heterodimer composed of PDGFA and PDGFB interacts with PDGFRB homodimers, and with heterodimers formed by PDGFRA and PDGFRB. Interacts with XLKD1 (By similarity).By similarity

GO - Molecular functioni

Protein-protein interaction databases

IntActiP31240. 1 interactor.
STRINGi10090.ENSMUSP00000000500.

Structurei

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DZ4model-A90-185[»]
2DZ5model-A90-185[»]
ProteinModelPortaliP31240.
SMRiP31240.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the PDGF/VEGF growth factor family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IJ45. Eukaryota.
ENOG410XWTB. LUCA.
HOGENOMiHOG000286027.
HOVERGENiHBG053546.
InParanoidiP31240.
KOiK17386.
PhylomeDBiP31240.
TreeFamiTF319554.

Family and domain databases

CDDicd00135. PDGF. 1 hit.
Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31240-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNRCWALFLP LCCYLRLVSA EGDPIPEELY EMLSDHSIRS FDDLQRLLHR
60 70 80 90 100
DSVDEDGAEL DLNMTRAHSG VELESSSRGR RSLGSLAAAE PAVIAECKTR
110 120 130 140 150
TEVFQISRNL IDRTNANFLV WPPCVEVQRC SGCCNNRNVQ CRASQVQMRP
160 170 180 190 200
VQVRKIEIVR KKPIFKKATV TLEDHLACKC ETIVTPRPVT RSPGTSREQR
210 220 230 240
AKTPQARVTI RTVRIRRPPK GKHRKFKHTH DKAALKETLG A
Length:241
Mass (Da):27,382
Last modified:July 1, 1993 - v1
Checksum:i3C5EB7A2DAD64178
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti183I → V in AAH53430 (PubMed:15489334).Curated1
Sequence conflicti183I → V in AAH64056 (PubMed:15489334).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84453
, M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
M64849
, M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
BC053430 mRNA. Translation: AAH53430.1.
BC064056 mRNA. Translation: AAH64056.1.
CCDSiCCDS27656.1.
PIRiA39073. PFMSGB.
RefSeqiNP_035187.2. NM_011057.3.
XP_017171991.1. XM_017316502.1.
UniGeneiMm.144089.

Genome annotation databases

GeneIDi18591.
KEGGimmu:18591.
UCSCiuc007wuz.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M84453
, M84448, M84449, M84450, M84451, M84452 Genomic DNA. Translation: AAA40113.1.
M64849
, M64844, M64845, M64846, M64847, M64848 Genomic DNA. Translation: AAA37485.1.
BC053430 mRNA. Translation: AAH53430.1.
BC064056 mRNA. Translation: AAH64056.1.
CCDSiCCDS27656.1.
PIRiA39073. PFMSGB.
RefSeqiNP_035187.2. NM_011057.3.
XP_017171991.1. XM_017316502.1.
UniGeneiMm.144089.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DZ4model-A90-185[»]
2DZ5model-A90-185[»]
ProteinModelPortaliP31240.
SMRiP31240.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP31240. 1 interactor.
STRINGi10090.ENSMUSP00000000500.

PTM databases

iPTMnetiP31240.
PhosphoSitePlusiP31240.

Proteomic databases

MaxQBiP31240.
PaxDbiP31240.
PeptideAtlasiP31240.
PRIDEiP31240.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi18591.
KEGGimmu:18591.
UCSCiuc007wuz.1. mouse.

Organism-specific databases

CTDi5155.
MGIiMGI:97528. Pdgfb.

Phylogenomic databases

eggNOGiENOG410IJ45. Eukaryota.
ENOG410XWTB. LUCA.
HOGENOMiHOG000286027.
HOVERGENiHBG053546.
InParanoidiP31240.
KOiK17386.
PhylomeDBiP31240.
TreeFamiTF319554.

Miscellaneous databases

PROiP31240.
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000000489.
CleanExiMM_PDGFB.

Family and domain databases

CDDicd00135. PDGF. 1 hit.
Gene3Di2.10.90.10. 1 hit.
InterProiIPR029034. Cystine-knot_cytokine.
IPR023581. PD_growth_factor_CS.
IPR000072. PDGF/VEGF_dom.
IPR006782. PDGF_N.
[Graphical view]
PfamiPF00341. PDGF. 1 hit.
PF04692. PDGF_N. 1 hit.
[Graphical view]
SMARTiSM00141. PDGF. 1 hit.
[Graphical view]
SUPFAMiSSF57501. SSF57501. 1 hit.
PROSITEiPS00249. PDGF_1. 1 hit.
PS50278. PDGF_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPDGFB_MOUSE
AccessioniPrimary (citable) accession number: P31240
Secondary accession number(s): Q6P3C4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 30, 2016
This is version 144 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.