P31231 (CASQ1_RANES) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: Calsequestrin-1 Alternative name(s): Calsequestrin, skeletal muscle isoform
Organism	Rana esculenta (Edible frog) (Pelophylax esculentus)
Taxonomic identifier	8401 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Pelophylax

Protein attributes

Sequence length	420 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calsequestrin is a high-capacity, moderate affinity, calcium-binding protein and thus acts as an internal calcium store in muscle. The release of calcium bound to calsequestrin through a calcium release channel triggers muscle contraction. The skeletal muscle CASQ1) binds around 80 Ca2+ ions, while the cardiac CASQ2) binds approximately 60 Ca2+ ions By similarity.
Subcellular location	Sarcoplasmic reticulum lumen. Note: This isoform of calsequestrin occurs in the sarcoplasmic reticulum's terminal cisternae luminal spaces of fast skeletal muscle cells.
Sequence similarities	Belongs to the calsequestrin family.

Ontologies

Keywords
Cellular component	Sarcoplasmic reticulum
Domain	Signal
Ligand	Calcium
Molecular function	Muscle protein
PTM	Glycoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Cellular_component	sarcoplasmic reticulum lumen Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 22	22	Ref.2
	Chain	23 – 420	398	Calsequestrin-1		PRO_0000004216
Regions
	Compositional bias	376 – 420	45	Asp/Glu-rich (acidic)
Amino acid modifications
	Glycosylation	338	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P31231 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 5589676478E09DF0	FASTA	420	48,205
	10 20 30 40 50 60 MKGPWLVLAA LCLSLANLGP RGEDGLDFPE YDGEDRVIHI SLKNYKAALK KYEVLALLYH 70 80 90 100 110 120 EPIGDDKASQ RQFEMEELIL ELAAQVLEDK GVGFGLVDSE DDAAVAKKLG LDEEDSIYVF 130 140 150 160 170 180 KDDEMIEYDG EFSADTLVEF LLDVLEDPVE FIDGSHELAA FENLDDEPKL IGYFKNEDSE 190 200 210 220 230 240 HYKAYEDAAE EFHPYIPFFA TFDAKVAKTL TLKLNEIDYY EPFHDEPITI PSKPNSEKEI 250 260 270 280 290 300 VDFLHQHKRP TLRKLRPDSM YETWEDDLNG IHIVAFAEED DPDGYEFLQI IKEVAEDNTD 310 320 330 340 350 360 NPDLSIIWID PEDFPLLIPY WEEKFGIDLS RPHIGVVNVT DADSVWMDMD DEEDLPTVDE 370 380 390 400 410 420 LEDWIEDVLE GEVNTEDDDD DDDDDDDDDD DDDDDDDDDD DDDDDDDDDD DDDDDDDDDD

References

[1]	"Molecular cloning, functional expression and tissue distribution of the cDNA encoding frog skeletal muscle calsequestrin." Treves S., Vilsen B., Chiozzi P., Andersen J.P., Zorzato F. Biochem. J. 283:767-772(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Muscle.
[2]	"Frog brain expresses a 60 KDa Ca2+ binding protein similar to mammalian calreticulin." Treveso S., Zorzato F., Chiozzi P., Melandri P., Volpe P., Pozzan T. Biochem. Biophys. Res. Commun. 175:444-450(1991) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 23-57. Tissue: Skeletal muscle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X64324 mRNA. Translation: CAA45609.1.
PIR	S22418.
3D structure databases
ProteinModelPortal	P31231.
SMR	P31231. Positions 25-369.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG050805.
Family and domain databases
Gene3D	3.40.30.10. 3 hits.
InterPro	IPR001393. Calsequestrin. IPR018233. Calsequestrin_CS. IPR012336. Thioredoxin-like_fold. [Graphical view]
PANTHER	PTHR10033. PTHR10033. 1 hit.
Pfam	PF01216. Calsequestrin. 1 hit. [Graphical view]
PRINTS	PR00312. CALSEQUESTRN.
SUPFAM	SSF52833. Thiordxn-like_fd. 3 hits.
PROSITE	PS00863. CALSEQUESTRIN_1. 1 hit. PS00864. CALSEQUESTRIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CASQ1_RANES

Accession

Primary (citable) accession number: P31231

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 66 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program