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Protein

Aminoacyl tRNA synthase complex-interacting multifunctional protein 1

Gene

Aimp1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-catalytic component of the multisynthase complex (PubMed:12060739). Stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase (By similarity). Binds tRNA. Possesses inflammatory cytokine activity (PubMed:1400342, PubMed:7545917). Negatively regulates TGF-beta signaling through stabilization of SMURF2 by binding to SMURF2 and inhibiting its SMAD7-mediated degradation (PubMed:18448069). Involved in glucose homeostasis through induction of glucagon secretion at low glucose levels (PubMed:17001013). Promotes dermal fibroblast proliferation and wound repair (PubMed:15681823). Regulates KDELR1-mediated retention of HSP90B1/gp96 in the endoplasmic reticulum (PubMed:17525271). Plays a role in angiogenesis by inducing endothelial cell migration at low concentrations and endothelian cell apoptosis at high concentrations (By similarity). Induces maturation of dendritic cells and monocyte cell adhesion (PubMed:18292511). Modulates endothelial cell responses by degrading HIF-1A through interaction with PSMA7 (By similarity).By similarity8 Publications

GO - Molecular functioni

  • cytokine activity Source: HGNC
  • GTPase binding Source: MGI
  • protein homodimerization activity Source: HGNC
  • tRNA binding Source: HGNC

GO - Biological processi

Keywordsi

Molecular functionCytokine, RNA-binding, tRNA-binding
Biological processAngiogenesis, Apoptosis, Cell adhesion, Inflammatory response, Protein biosynthesis

Protein family/group databases

MoonProtiP31230

Names & Taxonomyi

Protein namesi
Recommended name:
Aminoacyl tRNA synthase complex-interacting multifunctional protein 1
Alternative name(s):
Multisynthase complex auxiliary component p43
Cleaved into the following chain:
Alternative name(s):
Endothelial monocyte-activating polypeptide II1 Publication
Short name:
EMAP-II
Small inducible cytokine subfamily E member 1
Gene namesi
Name:Aimp1
Synonyms:Emap2, Scye1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:102774 Aimp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Endoplasmic reticulum, Golgi apparatus, Nucleus, Secreted

Pathology & Biotechi

Disruption phenotypei

Increased Hsp90b1 surface expression, dendritic cell hyperactivation and development of lupus-like autoimmune phenotypes (PubMed:17525271). Retarded growth after birth, reduced food intake, reduced plasma levels of glucose, free fatty acid, glucagon and insulin, increased glycogen content in the liver, and rapid decrease in blood glucose concentration upon fasting (PubMed:17001013).2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00002233952 – 310Aminoacyl tRNA synthase complex-interacting multifunctional protein 1Add BLAST309
ChainiPRO_0000019244145 – 310Endothelial monocyte-activating polypeptide 2Add BLAST166

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei138PhosphoserineBy similarity1
Modified residuei267N6-succinyllysineCombined sources1

Post-translational modificationi

Cleaved by caspase-7 in response to apoptosis to produce EMAP-II.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP31230
MaxQBiP31230
PaxDbiP31230
PeptideAtlasiP31230
PRIDEiP31230

PTM databases

iPTMnetiP31230
PhosphoSitePlusiP31230
SwissPalmiP31230

Expressioni

Tissue specificityi

Highly expressed in salivary glands and pancreatic alpha cells in the adult (at protein level) (PubMed:17001013). In the embryo, expressed primarily at sites of tissue remodeling such as ganglia, developing bones and teeth (PubMed:9770485).2 Publications

Inductioni

By wounding.1 Publication

Gene expression databases

CleanExiMM_SCYE1

Interactioni

Subunit structurei

Homodimer. Part of a multisubunit complex that groups tRNA ligases for Arg (RARS), Asp (DARS), Gln (QARS), Ile (IARS), Leu (LARS), Lys (KARS), Met (MARS) the bifunctional ligase for Glu and Pro (EPRS) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18 (PubMed:12060739). Interacts (via N-terminus) with RARS (via N-terminus). Part of a complex composed of RARS, QARS and AIMP1. Interacts (via C-terminus) with SMURF2 (PubMed:18448069). Interacts (via N-terminus) with HSP90B1/gp96 (via C-terminus) (PubMed:17525271). Interacts with PSMA7 (By similarity).By similarity3 Publications

GO - Molecular functioni

  • cytokine activity Source: HGNC
  • GTPase binding Source: MGI
  • protein homodimerization activity Source: HGNC

Protein-protein interaction databases

IntActiP31230, 3 interactors
MINTiP31230
STRINGi10090.ENSMUSP00000029663

Structurei

3D structure databases

ProteinModelPortaliP31230
SMRiP31230
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini149 – 250tRNA-bindingPROSITE-ProRule annotationAdd BLAST102

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni6 – 46Required for fibroblast proliferationBy similarityAdd BLAST41
Regioni54 – 192Interaction with HSP90B11 PublicationAdd BLAST139
Regioni101 – 115Required for endothelial cell deathBy similarityAdd BLAST15
Regioni115 – 190Required for endothelial cell migrationBy similarityAdd BLAST76

Phylogenomic databases

eggNOGiKOG2241 Eukaryota
COG0073 LUCA
InParanoidiP31230
PhylomeDBiP31230

Family and domain databases

InterProiView protein in InterPro
IPR012340 NA-bd_OB-fold
IPR002547 tRNA-bd_dom
PfamiView protein in Pfam
PF01588 tRNA_bind, 1 hit
SUPFAMiSSF50249 SSF50249, 1 hit
PROSITEiView protein in PROSITE
PS50886 TRBD, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31230-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATNDAVLKR LEQKGAEADQ IIEYLKQQVA LLKEKAILQA TMREEKKLRV
60 70 80 90 100
ENAKLKKEIE ELKQELILAE IHNGVEQVRV RLSTPLQTNC TASESVVQSP
110 120 130 140 150
SVATTASPAT KEQIKAGEEK KVKEKTEKKG EKKEKQQSAA ASTDSKPIDA
160 170 180 190 200
SRLDLRIGCI VTAKKHPDAD SLYVEEVDVG EAAPRTVVSG LVNHVPLEQM
210 220 230 240 250
QNRMVVLLCN LKPAKMRGVL SQAMVMCASS PEKVEILAPP NGSVPGDRIT
260 270 280 290 300
FDAFPGEPDK ELNPKKKIWE QIQPDLHTNA ECVATYKGAP FEVKGKGVCR
310
AQTMANSGIK
Length:310
Mass (Da):33,997
Last modified:October 18, 2001 - v2
Checksum:iA2F8FF52A33D03A0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U10118 mRNA Translation: AAA62203.1
BC002054 mRNA Translation: AAH02054.1
PIRiA55053
UniGeneiMm.235137

Similar proteinsi

Entry informationi

Entry nameiAIMP1_MOUSE
AccessioniPrimary (citable) accession number: P31230
Secondary accession number(s): Q60659
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: October 18, 2001
Last modified: April 25, 2018
This is version 134 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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