P31227 (SMOD_LITCT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names	Recommended name: S-modulin Alternative name(s): Sensitivity-modulating protein
Organism	Lithobates catesbeiana (American bullfrog) (Rana catesbeiana)
Taxonomic identifier	8400 [NCBI]
Taxonomic lineage	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amphibia › Batrachia › Anura › Neobatrachia › Ranoidea › Ranidae › Rana › Aquarana

Protein attributes

Sequence length	202 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Calcium-dependent regulator of light sensitivity of cGMP phosphodiesterase in rod outer segments. Control rhodopsin phosphorylation in a Ca2+-dependent manner.
Subcellular location	Note: The binding target of the Ca2+/S-modulin complex is possibly ROS membrane lipid(s).
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the recoverin family. Contains 4 EF-hand domains.

Ontologies

Keywords
Biological process	Sensory transduction Vision
Domain	Repeat
Ligand	Calcium Metal-binding
PTM	Lipoprotein Myristate
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological_process	response to stimulus Inferred from electronic annotation. Source: UniProtKB-KW visual perception Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	calcium ion binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 202	201	S-modulin		PRO_0000073799
Regions
	Domain	25 – 60	36	EF-hand 1
	Domain	61 – 96	36	EF-hand 2
	Domain	97 – 132	36	EF-hand 3
	Domain	147 – 182	36	EF-hand 4
	Calcium binding	74 – 85	12	1 Potential
	Calcium binding	110 – 121	12	2 Potential
Amino acid modifications
	Lipidation	2	1	N-myristoyl glycine By similarity
Experimental info
	Sequence conflict	91	1	M → H in BAA19728. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31227 [UniParc]. Last modified January 23, 2007. Version 4. Checksum: 5FFE379649904A2B	FASTA	202	23,527
	10 20 30 40 50 60 MGNTKSGALS KEILEELQLN TKFTQEELCT WYQSFLKECP SGRISKKQFE SIYSKFFPDA 70 80 90 100 110 120 DPKAYAQHVF RSFDANNDGT LDFKEYMIAL MMTSSGKANQ KLEWAFCLYD VDGNGTINKK 130 140 150 160 170 180 EVLEIITAIF KMINAEDQKH LPEDENTPEK RTNKIWVYFG KKDDDKLTEG EFIQGIVKNK 190 200 EILRLIQYEP QKVKDKLKEK KH

References

[1]	"Recoverin has S-modulin activity in frog rods." Kawamura S., Hisatomi O., Kayada S., Tokunaga F., Kuo C.-H. J. Biol. Chem. 268:14579-14582(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Retina.
[2]	"Purification and characterization of S-modulin, a calcium-dependent regulator on cGMP phosphodiesterase in frog rod photoreceptors." Kawamura S., Takamatsu K., Kitamura K. Biochem. Biophys. Res. Commun. 186:411-417(1992) [PubMed] [Europe PMC] [Abstract] Cited for: PROTEIN SEQUENCE OF 22-28; 47-55; 63-97; 131-150; 154-162; 167-177 AND 180-192. Tissue: Retina.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D83641 mRNA. Translation: BAA19728.1.
PIR	JC1107. T10531.
3D structure databases
ProteinModelPortal	P31227.
SMR	P31227. Positions 2-191.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
HOVERGEN	HBG108179.
Family and domain databases
Gene3D	1.10.238.10. 3 hits.
InterPro	IPR011992. EF-hand-like_dom. IPR018247. EF_Hand_1_Ca_BS. IPR002048. EF_hand_dom. IPR001125. Recoverin. [Graphical view]
Pfam	PF13202. EF_hand_3. 1 hit. PF13499. EF_hand_5. 1 hit. [Graphical view]
PRINTS	PR00450. RECOVERIN.
SMART	SM00054. EFh. 2 hits. [Graphical view]
PROSITE	PS00018. EF_HAND_1. 2 hits. PS50222. EF_HAND_2. 3 hits. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SMOD_LITCT

Accession

Primary (citable) accession number: P31227

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	January 23, 2007
Last modified:	May 1, 2013
This is version 76 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program