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P31214

- S5A2_RAT

UniProt

P31214 - S5A2_RAT

Protein

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Gene

Srd5a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 104 (01 Oct 2014)
      Sequence version 1 (01 Jul 1993)
      Previous versions | rss
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    Functioni

    Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology By similarity.By similarity

    Catalytic activityi

    A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH.1 Publication

    Kineticsi

    1. KM=74 nM for testosterone1 Publication
    2. KM=42 nM for progesterone1 Publication
    3. KM=170 nM for androstenedione1 Publication
    4. KM=376 nM for corticosterone1 Publication
    5. KM=11.2 µM for cortisol1 Publication

    Vmax=0.5 nmol/min/mg enzyme1 Publication

    pH dependencei

    Optimally active at acidic pHs.1 Publication

    GO - Molecular functioni

    1. 3-oxo-5-alpha-steroid 4-dehydrogenase activity Source: RGD
    2. amide binding Source: RGD
    3. cholestenone 5-alpha-reductase activity Source: UniProtKB-EC

    GO - Biological processi

    1. androgen biosynthetic process Source: RGD
    2. androgen metabolic process Source: RGD
    3. biphenyl metabolic process Source: RGD
    4. bone development Source: RGD
    5. cell differentiation Source: UniProtKB-KW
    6. dibenzo-p-dioxin metabolic process Source: RGD
    7. female genitalia development Source: RGD
    8. hippocampus development Source: RGD
    9. hypothalamus development Source: RGD
    10. male genitalia development Source: RGD
    11. male gonad development Source: RGD
    12. phthalate metabolic process Source: RGD
    13. response to drug Source: RGD
    14. response to follicle-stimulating hormone Source: RGD
    15. response to nutrient levels Source: RGD
    16. response to organic cyclic compound Source: RGD
    17. response to organic substance Source: RGD
    18. response to peptide hormone Source: RGD
    19. response to steroid hormone Source: RGD
    20. response to testosterone Source: RGD
    21. sex differentiation Source: RGD
    22. steroid biosynthetic process Source: RGD
    23. steroid catabolic process Source: RGD

    Keywords - Molecular functioni

    Oxidoreductase

    Keywords - Biological processi

    Differentiation, Sexual differentiation

    Keywords - Ligandi

    NADP

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxo-5-alpha-steroid 4-dehydrogenase 2 (EC:1.3.1.22)
    Alternative name(s):
    5 alpha-SR2
    SR type 2
    Steroid 5-alpha-reductase 2
    Short name:
    S5AR 2
    Gene namesi
    Name:Srd5a2
    OrganismiRattus norvegicus (Rat)
    Taxonomic identifieri10116 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
    ProteomesiUP000002494: Chromosome 6

    Organism-specific databases

    RGDi621480. Srd5a2.

    Subcellular locationi

    GO - Cellular componenti

    1. cell body fiber Source: RGD
    2. cytoplasm Source: RGD
    3. endoplasmic reticulum membrane Source: UniProtKB-SubCell
    4. integral component of membrane Source: UniProtKB-KW
    5. neuronal cell body Source: RGD

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane, Microsome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 2542543-oxo-5-alpha-steroid 4-dehydrogenase 2PRO_0000213680Add
    BLAST

    Proteomic databases

    PRIDEiP31214.

    Expressioni

    Tissue specificityi

    Expressed in high levels in the prostate and many other androgen-sensitive tissues.1 Publication

    Gene expression databases

    GenevestigatoriP31214.

    Interactioni

    Protein-protein interaction databases

    STRINGi10116.ENSRNOP00000009254.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei8 – 2821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei72 – 9221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei146 – 16621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei206 – 22621HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the steroid 5-alpha reductase family.Curated

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiNOG282706.
    GeneTreeiENSGT00510000046634.
    HOGENOMiHOG000050133.
    HOVERGENiHBG003402.
    InParanoidiP31214.
    KOiK12344.
    OMAiHHRYYLK.
    OrthoDBiEOG75QR56.
    PhylomeDBiP31214.
    TreeFamiTF314668.

    Family and domain databases

    InterProiIPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view]
    PfamiPF02544. Steroid_dh. 1 hit.
    [Graphical view]
    PIRSFiPIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEiPS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31214-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQIVCHQVPV LAGSATLATM GTLILCLGKP ASYGKHTESV SSGVPFLPAR    50
    IAWFLQELPS FVVSVGMLAW QPRSLFGPPG NVLLALFSAH YFHRTFIYSL 100
    LTRGRPFPAV LFLRATAFCI GNGLLQAYYL VYCAEYPEEW YTDVRFSFGV 150
    FLFILGMGIN IHSDYTLRQL RKPGEVIYRI PRGGLFTYVS GANFLGEIIE 200
    WIGYALATWS VPAFAFAFFT LCFLGMQAFY HHRFYLKMFK DYPKSRKALI 250
    PFIF 254
    Length:254
    Mass (Da):28,772
    Last modified:July 1, 1993 - v1
    Checksum:i8874C533DF3BD123
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95058 mRNA. Translation: AAA42182.1.
    PIRiA44104.
    RefSeqiNP_073202.1. NM_022711.4.
    UniGeneiRn.9938.

    Genome annotation databases

    EnsembliENSRNOT00000008983; ENSRNOP00000009254; ENSRNOG00000027042.
    GeneIDi64677.
    KEGGirno:64677.
    UCSCiRGD:621480. rat.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M95058 mRNA. Translation: AAA42182.1 .
    PIRi A44104.
    RefSeqi NP_073202.1. NM_022711.4.
    UniGenei Rn.9938.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 10116.ENSRNOP00000009254.

    Chemistry

    BindingDBi P31214.
    ChEMBLi CHEMBL5099.

    Proteomic databases

    PRIDEi P31214.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSRNOT00000008983 ; ENSRNOP00000009254 ; ENSRNOG00000027042 .
    GeneIDi 64677.
    KEGGi rno:64677.
    UCSCi RGD:621480. rat.

    Organism-specific databases

    CTDi 6716.
    RGDi 621480. Srd5a2.

    Phylogenomic databases

    eggNOGi NOG282706.
    GeneTreei ENSGT00510000046634.
    HOGENOMi HOG000050133.
    HOVERGENi HBG003402.
    InParanoidi P31214.
    KOi K12344.
    OMAi HHRYYLK.
    OrthoDBi EOG75QR56.
    PhylomeDBi P31214.
    TreeFami TF314668.

    Miscellaneous databases

    NextBioi 613680.
    PROi P31214.

    Gene expression databases

    Genevestigatori P31214.

    Family and domain databases

    InterProi IPR016636. 3-oxo-5-alpha-steroid_4-DH.
    IPR001104. 3-oxo-5_a-steroid_4-DH_C.
    [Graphical view ]
    Pfami PF02544. Steroid_dh. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF015596. 5_alpha-SR2. 1 hit.
    PROSITEi PS50244. S5A_REDUCTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Tissue distribution and kinetic characteristics of rat steroid 5 alpha-reductase isozymes. Evidence for distinct physiological functions."
      Normington K., Russell D.W.
      J. Biol. Chem. 267:19548-19554(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY.
      Tissue: Testis.

    Entry informationi

    Entry nameiS5A2_RAT
    AccessioniPrimary (citable) accession number: P31214
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: July 1, 1993
    Last modified: October 1, 2014
    This is version 104 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3