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Protein

3-oxo-5-alpha-steroid 4-dehydrogenase 2

Gene

Srd5a2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids (PubMed:1527072). It plays a central role in sexual differentiation and androgen physiology (By similarity).By similarity1 Publication

Catalytic activityi

A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta4-steroid + NADPH.1 Publication

Kineticsi

  1. KM=74 nM for testosterone1 Publication
  2. KM=42 nM for progesterone1 Publication
  3. KM=170 nM for androstenedione1 Publication
  4. KM=376 nM for corticosterone1 Publication
  5. KM=11.2 µM for cortisol1 Publication
  1. Vmax=0.5 nmol/min/mg enzyme1 Publication

pH dependencei

Optimally active at acidic pHs.1 Publication

GO - Molecular functioni

GO - Biological processi

  • androgen biosynthetic process Source: RGD
  • androgen metabolic process Source: RGD
  • biphenyl metabolic process Source: RGD
  • bone development Source: RGD
  • cell differentiation Source: UniProtKB-KW
  • dibenzo-p-dioxin metabolic process Source: RGD
  • female genitalia development Source: RGD
  • hippocampus development Source: RGD
  • hypothalamus development Source: RGD
  • male genitalia development Source: RGD
  • male gonad development Source: RGD
  • phthalate metabolic process Source: RGD
  • progesterone metabolic process Source: GO_Central
  • response to drug Source: RGD
  • response to follicle-stimulating hormone Source: RGD
  • response to nutrient levels Source: RGD
  • response to organic cyclic compound Source: RGD
  • response to organic substance Source: RGD
  • response to peptide hormone Source: RGD
  • response to steroid hormone Source: RGD
  • response to testosterone Source: RGD
  • sex differentiation Source: RGD
  • steroid biosynthetic process Source: RGD
  • steroid catabolic process Source: RGD
  • testosterone biosynthetic process Source: UniProtKB

Keywordsi

Molecular functionOxidoreductase
Biological processDifferentiation, Sexual differentiation
LigandNADP

Enzyme and pathway databases

BRENDAi1.3.1.22 5301
ReactomeiR-RNO-193048 Androgen biosynthesis
SABIO-RKiP31214

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 2 (EC:1.3.1.221 Publication)
Alternative name(s):
5 alpha-SR2
SR type 2
Steroid 5-alpha-reductase 2
Short name:
S5AR 2
Gene namesi
Name:Srd5a2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 6

Organism-specific databases

RGDi621480 Srd5a2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei8 – 28HelicalSequence analysisAdd BLAST21
Transmembranei72 – 92HelicalSequence analysisAdd BLAST21
Transmembranei146 – 166HelicalSequence analysisAdd BLAST21
Transmembranei206 – 226HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Endoplasmic reticulum, Membrane, Microsome

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5099

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002136801 – 2543-oxo-5-alpha-steroid 4-dehydrogenase 2Add BLAST254

Proteomic databases

PaxDbiP31214
PRIDEiP31214

Expressioni

Tissue specificityi

Expressed in high levels in the prostate and many other androgen-sensitive tissues.1 Publication

Gene expression databases

BgeeiENSRNOG00000027042
GenevisibleiP31214 RN

Interactioni

Protein-protein interaction databases

STRINGi10116.ENSRNOP00000009254

Chemistry databases

BindingDBiP31214

Family & Domainsi

Sequence similaritiesi

Belongs to the steroid 5-alpha reductase family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG1638 Eukaryota
ENOG410XS6P LUCA
GeneTreeiENSGT00510000046634
HOGENOMiHOG000050133
HOVERGENiHBG003402
InParanoidiP31214
KOiK12344
OMAiEYPAEWY
OrthoDBiEOG091G0KU4
PhylomeDBiP31214
TreeFamiTF314668

Family and domain databases

InterProiView protein in InterPro
IPR016636 3-oxo-5-alpha-steroid_4-DH
IPR001104 3-oxo-5_a-steroid_4-DH_C
PfamiView protein in Pfam
PF02544 Steroid_dh, 1 hit
PIRSFiPIRSF015596 5_alpha-SR2, 1 hit
PROSITEiView protein in PROSITE
PS50244 S5A_REDUCTASE, 1 hit

Sequencei

Sequence statusi: Complete.

P31214-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQIVCHQVPV LAGSATLATM GTLILCLGKP ASYGKHTESV SSGVPFLPAR
60 70 80 90 100
IAWFLQELPS FVVSVGMLAW QPRSLFGPPG NVLLALFSAH YFHRTFIYSL
110 120 130 140 150
LTRGRPFPAV LFLRATAFCI GNGLLQAYYL VYCAEYPEEW YTDVRFSFGV
160 170 180 190 200
FLFILGMGIN IHSDYTLRQL RKPGEVIYRI PRGGLFTYVS GANFLGEIIE
210 220 230 240 250
WIGYALATWS VPAFAFAFFT LCFLGMQAFY HHRFYLKMFK DYPKSRKALI

PFIF
Length:254
Mass (Da):28,772
Last modified:July 1, 1993 - v1
Checksum:i8874C533DF3BD123
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M95058 mRNA Translation: AAA42182.1
PIRiA44104
RefSeqiNP_073202.1, NM_022711.4
UniGeneiRn.9938

Genome annotation databases

EnsembliENSRNOT00000008983; ENSRNOP00000009254; ENSRNOG00000027042
GeneIDi64677
KEGGirno:64677
UCSCiRGD:621480 rat

Similar proteinsi

Entry informationi

Entry nameiS5A2_RAT
AccessioniPrimary (citable) accession number: P31214
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 23, 2018
This is version 125 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

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