P31214 (S5A2_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxo-5-alpha-steroid 4-dehydrogenase 2
EC=1.3.1.22

Alternative name(s):
5 alpha-SR2
SR type 2
Steroid 5-alpha-reductase 2
Short name=S5AR 2

Gene names

Name:

Srd5a2

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology By similarity.
Catalytic activity	A 3-oxo-5-alpha-steroid + NADP+ = a 3-oxo-Delta(4)-steroid + NADPH. Ref.1
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed in high levels in the prostate and many other androgen-sensitive tissues. Ref.1
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
Biophysicochemical properties	Kinetic parameters: KM=74 nM for testosterone Ref.1 KM=42 nM for progesterone KM=170 nM for androstenedione KM=376 nM for corticosterone KM=11.2 µM for cortisol Vmax=0.5 nmol/min/mg enzyme pH dependence: Optimally active at acidic pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane Transmembrane helix
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	androgen biosynthetic process Inferred from direct assay PubMed 12606426. Source: RGD biphenyl metabolic process Inferred from expression pattern PubMed 16938428. Source: RGD bone development Inferred from expression pattern PubMed 15012600. Source: RGD cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW dibenzo-p-dioxin metabolic process Inferred from expression pattern PubMed 11222880. Source: RGD female genitalia development Inferred from expression pattern PubMed 9142501. Source: RGD hippocampus development Inferred from expression pattern PubMed 21047951. Source: RGD hypothalamus development Inferred from expression pattern PubMed 15804399. Source: RGD male genitalia development Inferred from expression pattern PubMed 9142501. Source: RGD male gonad development Inferred from expression pattern PubMed 12606426. Source: RGD phthalate metabolic process Inferred from expression pattern PubMed 20954080. Source: RGD response to drug Inferred from expression pattern PubMed 18978642. Source: RGD response to follicle-stimulating hormone stimulus Inferred from expression pattern PubMed 12630924. Source: RGD response to nutrient levels Inferred from expression pattern PubMed 16425201. Source: RGD response to peptide hormone stimulus Inferred from direct assay PubMed 12630924. Source: RGD response to testosterone stimulus Inferred from expression pattern PubMed 16595696. Source: RGD steroid catabolic process Inferred from direct assay Ref.1. Source: RGD
Cellular_component	cell body fiber Inferred from direct assay PubMed 15305365. Source: RGD cytoplasm Inferred from direct assay PubMed 15305365. Source: RGD endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW neuronal cell body Inferred from direct assay PubMed 15305365. Source: RGD
Molecular_function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Inferred from direct assay Ref.1PubMed 7662592. Source: RGD amide binding Inferred from physical interaction PubMed 9328210. Source: RGD cholestenone 5-alpha-reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	3-oxo-5-alpha-steroid 4-dehydrogenase 2		PRO_0000213680
Regions
	Transmembrane	8 – 28	21	Helical; Potential
	Transmembrane	72 – 92	21	Helical; Potential
	Transmembrane	146 – 166	21	Helical; Potential
	Transmembrane	206 – 226	21	Helical; Potential

Sequences

	Sequence		Length	Mass (Da)
	P31214 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 8874C533DF3BD123	FASTA	254	28,772
	10 20 30 40 50 60 MQIVCHQVPV LAGSATLATM GTLILCLGKP ASYGKHTESV SSGVPFLPAR IAWFLQELPS 70 80 90 100 110 120 FVVSVGMLAW QPRSLFGPPG NVLLALFSAH YFHRTFIYSL LTRGRPFPAV LFLRATAFCI 130 140 150 160 170 180 GNGLLQAYYL VYCAEYPEEW YTDVRFSFGV FLFILGMGIN IHSDYTLRQL RKPGEVIYRI 190 200 210 220 230 240 PRGGLFTYVS GANFLGEIIE WIGYALATWS VPAFAFAFFT LCFLGMQAFY HHRFYLKMFK 250 DYPKSRKALI PFIF

References

[1]	"Tissue distribution and kinetic characteristics of rat steroid 5 alpha-reductase isozymes. Evidence for distinct physiological functions." Normington K., Russell D.W. J. Biol. Chem. 267:19548-19554(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], CATALYTIC ACTIVITY, BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Tissue: Testis.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M95058 mRNA. Translation: AAA42182.1.
IPI	IPI00210825.
PIR	A44104.
RefSeq	NP_073202.1. NM_022711.4.
UniGene	Rn.9938.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	10116.ENSRNOP00000009254.
Proteomic databases
PRIDE	P31214.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSRNOT00000008983; ENSRNOP00000009254; ENSRNOG00000027042.
GeneID	64677.
KEGG	rno:64677.
UCSC	RGD:621480. rat.
Organism-specific databases
CTD	6716.
RGD	621480. Srd5a2.
Phylogenomic databases
eggNOG	NOG282706.
GeneTree	ENSGT00510000046634.
HOGENOM	HOG000050133.
HOVERGEN	HBG003402.
InParanoid	P31214.
KO	K12344.
OMA	FAFFTLC.
OrthoDB	EOG469QVK.
Gene expression databases
ArrayExpress	P31214.
Genevestigator	P31214.
GermOnline	ENSRNOG00000027042. Rattus norvegicus.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P31214.
ChEMBL	CHEMBL5099.
NextBio	613680.

Entry information

Entry name

S5A2_RAT

Accession

Primary (citable) accession number: P31214

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 95 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program