Reviewed, UniProtKB/Swiss-Prot P31214 (S5A2_RAT)

Names and origin

Protein names

Recommended name:
    3-oxo-5-alpha-steroid 4-dehydrogenase 2
    EC=1.3.99.5
Alternative name(s):
    Steroid 5-alpha-reductase 2
    5 alpha-SR2
    SR type 2

Gene names

Name:

Srd5a2

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	254 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Converts testosterone (T) into 5-alpha-dihydrotestosterone (DHT) and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology By similarity.
Catalytic activity	A 3-oxo-5-alpha-steroid + acceptor = a 3-oxo-Delta(4)-steroid + reduced acceptor.
Subcellular location	Microsome membrane; Multi-pass membrane protein. Endoplasmic reticulum membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed in high levels in the prostate and many other androgen-sensitive tissues. Ref.1
Sequence similarities	Belongs to the steroid 5-alpha reductase family.
biophysicochemical properties	pH dependence: Optimally active at acidic pHs.

Ontologies

Keywords
Biological process	Differentiation Sexual differentiation
Cellular component	Endoplasmic reticulum Membrane Microsome
Domain	Transmembrane
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	cell differentiation Inferred from electronic annotation. Source: UniProtKB-KW male genitalia development Ref.1 Traceable author statement. Source: RGD oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW response to drug Inferred from direct assay. Source: RGD response to organic substance Inferred from direct assay. Source: RGD response to peptide hormone stimulus Inferred from direct assay. Source: RGD response to steroid hormone stimulus Inferred from expression pattern. Source: RGD sex determination Inferred from electronic annotation. Source: UniProtKB-KW steroid catabolic process Ref.1 Inferred from direct assay. Source: RGD
Cellular component	endoplasmic reticulum membrane Inferred from electronic annotation. Source: UniProtKB-SubCell integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW microsome Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxo-5-alpha-steroid 4-dehydrogenase activity Ref.1 Inferred from direct assay. Source: RGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 254	254	3-oxo-5-alpha-steroid 4-dehydrogenase 2		PRO_0000213680
Regions
	Transmembrane	8 – 28	21	Potential
	Transmembrane	72 – 92	21	Potential
	Transmembrane	146 – 166	21	Potential
	Transmembrane	206 – 226	21	Potential

Sequences

	Sequence		Length	Mass (Da)
	P31214-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 8874C533DF3BD123	FASTA	254	28,772
	10 20 30 40 50 60 MQIVCHQVPV LAGSATLATM GTLILCLGKP ASYGKHTESV SSGVPFLPAR IAWFLQELPS 70 80 90 100 110 120 FVVSVGMLAW QPRSLFGPPG NVLLALFSAH YFHRTFIYSL LTRGRPFPAV LFLRATAFCI 130 140 150 160 170 180 GNGLLQAYYL VYCAEYPEEW YTDVRFSFGV FLFILGMGIN IHSDYTLRQL RKPGEVIYRI 190 200 210 220 230 240 PRGGLFTYVS GANFLGEIIE WIGYALATWS VPAFAFAFFT LCFLGMQAFY HHRFYLKMFK 250 DYPKSRKALI PFIF

References

[1]

"Tissue distribution and kinetic characteristics of rat steroid 5 alpha-reductase isozymes. Evidence for distinct physiological functions." Normington K., Russell D.W. J. Biol. Chem. 267:19548-19554(1992) [PubMed: 1527072] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], BIOPHYSICOCHEMICAL PROPERTIES, TISSUE SPECIFICITY. Tissue: Testis.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M95058 mRNA. Translation: AAA42182.1.
IPI	IPI00210825.
PIR	A44104.
RefSeq	NP_073202.1.
UniGene	Rn.9938
3D structure databases
ModBase	Search...
Genome annotation databases
Ensembl	ENSRNOG00000027042. Rattus norvegicus. [Contig view]
GeneID	64677.
KEGG	rno:64677.
Organism-specific databases
RGD	621480. Srd5a2.
Phylogenomic databases
HOVERGEN	P31214.
OMA	P31214. EITYRIP.
Enzyme and pathway databases
BRENDA	1.3.99.5. 248.
Gene expression databases
ArrayExpress	P31214.
GermOnline	ENSRNOG00000027042. Rattus norvegicus.
Family and domain databases
InterPro	IPR016636. 3-oxo-5-alpha-steroid_4-DH. IPR001104. 3-oxo-5_a-steroid_4-DH_C. [Graphical view]
Pfam	PF02544. Steroid_dh. 1 hit. [Graphical view]
PIRSF	PIRSF015596. 5_alpha-SR2. 1 hit.
PROSITE	PS50244. S5A_REDUCTASE. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	613680.

Entry information

Entry name

S5A2_RAT

Accession

Primary (citable) accession number: P31214

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 64 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)