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P31211 (CBG_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 108. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Corticosteroid-binding globulin

Short name=CBG
Alternative name(s):
Serpin A6
Transcortin
Gene names
Name:Serpina6
Synonyms:Cbg
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Major transport protein for glucocorticoids and progestins in the blood of almost all vertebrate species. Ref.5

Subcellular location

Secreted.

Tissue specificity

Expressed by the liver; secreted in plasma. Ref.1

Domain

Proteolytic cleavage leads to an important conformation change. This reduces the affinity for steroids By similarity.

Sequence similarities

Belongs to the serpin family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Ref.4
Chain23 – 396374Corticosteroid-binding globulin
PRO_0000032432

Sites

Binding site2461Corticosteroid
Binding site2781Corticosteroid
Binding site3841Corticosteroid
Site2421Conserved cysteine within steroid binding domain

Amino acid modifications

Glycosylation881N-linked (GlcNAc...) Potential
Glycosylation2161N-linked (GlcNAc...) Potential
Glycosylation2521N-linked (GlcNAc...) Potential
Glycosylation3191N-linked (GlcNAc...) Potential
Glycosylation3521N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis321R → A: Loss of corticosteroid binding. Ref.5
Mutagenesis361P → A: Increased affinity for corticosteroids. Ref.5
Mutagenesis2461Q → A: Loss of corticosteroid binding. Ref.5
Mutagenesis2561F → A: Loss of corticosteroid binding. Ref.5
Mutagenesis2781D → A: Loss of corticosteroid binding. Ref.5
Sequence conflict47 – 482QR → EC AA sequence Ref.4
Sequence conflict541P → C AA sequence Ref.4
Sequence conflict163 – 1642NQ → TR Ref.1
Sequence conflict2981I → M Ref.1
Sequence conflict3071M → V Ref.1

Secondary structure

............................................................ 396
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P31211 [UniParc].

Last modified September 2, 2008. Version 2.
Checksum: A3D12AEC876D2BA2

FASTA39644,671
        10         20         30         40         50         60 
MSLALYTCLL WLCTSGLWTA QASTNESSNS HRGLAPTNVD FAFNLYQRLV ALNPDKNTLI 

        70         80         90        100        110        120 
SPVSISMALA MVSLGSAQTQ SLQSLGFNLT ETSEAEIHQS FQYLNYLLKQ SDTGLEMNMG 

       130        140        150        160        170        180 
NAMFLLQKLK LKDSFLADVK QYYESEALAI DFEDWTKASQ QINQHVKDKT QGKIEHVFSD 

       190        200        210        220        230        240 
LDSPASFILV NYIFLRGIWE LPFSPENTRE EDFYVNETST VKVPMMVQSG SIGYFRDSVF 

       250        260        270        280        290        300 
PCQLIQMDYV GNGTAFFILP DQGQMDTVIA ALSRDTIDRW GKLMTPRQVN LYIPKFSISD 

       310        320        330        340        350        360 
TYDLKDMLED LNIKDLLTNQ SDFSGNTKDV PLTLTMVHKA MLQLDEGNVL PNSTNGAPLH 

       370        380        390 
LRSEPLDIKF NKPFILLLFD KFTWSSLMMS QVVNPA 

« Hide

References

« Hide 'large scale' references
[1]"Rat corticosteroid binding globulin: primary structure and messenger ribonucleic acid levels in the liver under different physiological conditions."
Smith C.L., Hammond G.L.
Mol. Endocrinol. 3:420-426(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], TISSUE SPECIFICITY.
Tissue: Liver.
[2]Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Brown Norway.
[3]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Liver.
[4]"Comparative structural analyses of corticosteroid binding globulin."
Kato E.A., Hsu B.R.-S., Kuhn R.W.
J. Steroid Biochem. 29:213-220(1988) [PubMed] [Europe PMC] [Abstract]
Cited for: PROTEIN SEQUENCE OF 23-60.
[5]"Corticosteroid-binding globulin, a structural basis for steroid transport and proteinase-triggered release."
Klieber M.A., Underhill C., Hammond G.L., Muller Y.A.
J. Biol. Chem. 282:29594-29603(2007) [PubMed] [Europe PMC] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 27-396 IN COMPLEX WITH CORTICOSTEROID, FUNCTION, MUTAGENESIS OF ARG-32; PRO-36; GLN-246; PHE-256 AND ASP-278.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CH473982 Genomic DNA. Translation: EDL81783.1.
BC088300 mRNA. Translation: AAH88300.1.
PIRA40066.
RefSeqNP_001009663.1. NM_001009663.1.
UniGeneRn.2374.

3D structure databases

PDBe
RCSB-PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
2V95X-ray1.93A27-396[»]
ProteinModelPortalP31211.
SMRP31211. Positions 29-396.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING10116.ENSRNOP00000012500.

Proteomic databases

PaxDbP31211.
PRIDEP31211.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSRNOT00000012500; ENSRNOP00000012500; ENSRNOG00000009438.
GeneID299270.
KEGGrno:299270.
UCSCRGD:1595901. rat.

Organism-specific databases

CTD866.
RGD1595901. Serpina6.

Phylogenomic databases

eggNOGCOG4826.
GeneTreeENSGT00740000115120.
HOGENOMHOG000238521.
HOVERGENHBG005957.
InParanoidQ5M822.
OMASTREENF.
OrthoDBEOG7J446W.
PhylomeDBP31211.
TreeFamTF343201.

Gene expression databases

GenevestigatorP31211.

Family and domain databases

InterProIPR023795. Serpin_CS.
IPR023796. Serpin_dom.
IPR000215. Serpin_fam.
[Graphical view]
PANTHERPTHR11461. PTHR11461. 1 hit.
PfamPF00079. Serpin. 1 hit.
[Graphical view]
SMARTSM00093. SERPIN. 1 hit.
[Graphical view]
SUPFAMSSF56574. SSF56574. 1 hit.
PROSITEPS00284. SERPIN. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP31211.
NextBio645130.
PROP31211.

Entry information

Entry nameCBG_RAT
AccessionPrimary (citable) accession number: P31211
Secondary accession number(s): Q5M822
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: September 2, 2008
Last modified: July 9, 2014
This is version 108 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PDB cross-references

Index of Protein Data Bank (PDB) cross-references