P31210 (AK1D1_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
3-oxo-5-beta-steroid 4-dehydrogenase
EC=1.3.1.3

Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase

Gene names

Name:

Akr1d1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
Catalytic activity	5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH. 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Bile acid catabolism Lipid metabolism Steroid metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological process	bile acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	3-oxo-5-beta-steroid 4-dehydrogenase activity Inferred from direct assay Ref.2. Source: MGI delta4-3-oxosteroid 5beta-reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	3-oxo-5-beta-steroid 4-dehydrogenase		PRO_0000124672
Regions
	Nucleotide binding	168 – 169	2	NADP By similarity
	Nucleotide binding	220 – 224	5	NADP By similarity
	Nucleotide binding	273 – 283	11	NADP By similarity
Sites
	Active site	57	1	Proton donor By similarity
	Binding site	52	1	NADP By similarity
	Binding site	192	1	NADP By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31210 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 32D8266BFA62CE99	FASTA	326	37,378
	10 20 30 40 50 60 MNLSTANHHI PLNDGNSIPI IGLGTYSDPR PVPGKTFIAV KTAIDEGYRH IDGAYVYRNE 70 80 90 100 110 120 HEVGEAIREK VAEGKVKREE IFYCGKLWST DHDPEMVRPA LERTLQTLKL DYIDLYIIEM 130 140 150 160 170 180 PMAFKPGEEF YPKDENGRVI YHKSNLCATW EALEACKDAG LVKSLGVSNF NRRQLEVILN 190 200 210 220 230 240 KPGLKYKPVT NQVECHPYFT QTKLLEVSAS SMTSFIVAYS PLGTCRNPLW VNVSSPPLLK 250 260 270 280 290 300 DELLTSLGKK YNKTQAQIVL RFDIQRGLVV IPKSTTPERI KENFQIFDFS LTKEEMKDIE 310 320 ALNKNVRFVE MLMWSDHPEY PFHDEY

References

[1]	"Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver." Onishi Y., Noshiro M., Shimosato T., Okuda K. FEBS Lett. 283:215-218(1991) [PubMed: 1710579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Delta 4-3-Oxosteroid 5 beta-reductase. Structure and function." Onishi Y., Noshiro M., Shimosato T., Okuda K. Biol. Chem. Hoppe-Seyler 372:1039-1049(1991) [PubMed: 1789929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[3]	"Identification of the 37-kd rat liver protein that forms an acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5 beta-reductase." Zhu Y., Fillenwarth M.J., Crabb D., Lumeng L., Lin R.C. Hepatology 23:115-122(1996) [PubMed: 8550030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D17309 mRNA. Translation: BAA04131.1. S80431 mRNA. Translation: AAB35916.2.
IPI	IPI00210823.
PIR	S15835.
RefSeq	NP_620239.1. NM_138884.1.
UniGene	Rn.25716.
3D structure databases
ProteinModelPortal	P31210.
SMR	P31210. Positions 2-326.
ModBase	Search...
Protein-protein interaction databases
STRING	P31210.
PTM databases
PhosphoSite	P31210.
Proteomic databases
PRIDE	P31210.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	192242.
KEGG	rno:192242.
UCSC	S80431. rat.
Organism-specific databases
CTD	6718.
RGD	620752. Akr1d1.
Phylogenomic databases
eggNOG	roNOG13906.
GeneTree	ENSGT00550000074107.
HOVERGEN	HBG000020.
InParanoid	P31210.
OrthoDB	EOG4Q2DG2.
PhylomeDB	P31210.
Enzyme and pathway databases
BioCyc	MetaCyc:MONOMER-14304.
Gene expression databases
ArrayExpress	P31210.
Genevestigator	P31210.
GermOnline	ENSRNOG00000013004. Rattus norvegicus.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. IPR020471. Aldo/keto_reductase_subgr. IPR023210. NADP_OxRdtase_dom. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
KO	K00251.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
PIRSF	PIRSF000097. AKR. 1 hit.
PRINTS	PR00069. ALDKETRDTASE.
SUPFAM	SSF51430. Aldo/ket_red. 1 hit.
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	622866.

Entry information

Entry name

AK1D1_RAT

Accession

Primary (citable) accession number: P31210

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	November 16, 2011
This is version 89 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program