Reviewed, UniProtKB/Swiss-Prot P31210 (AK1D1_RAT)

Names and origin

Protein names

Recommended name:
    3-oxo-5-beta-steroid 4-dehydrogenase
    EC=1.3.1.3
Alternative name(s):
    Delta(4)-3-ketosteroid 5-beta-reductase
    Delta(4)-3-oxosteroid 5-beta-reductase
    Aldo-keto reductase family 1 member D1

Gene names

Name:

Akr1d1

Organism

Rattus norvegicus (Rat)

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.
Catalytic activity	5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH. 17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone.
Subcellular location	Cytoplasm.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the aldo/keto reductase family.

Ontologies

Keywords
Biological process	Bile acid catabolism Lipid metabolism Steroid metabolism
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	bile acid catabolic process Inferred from electronic annotation. Source: UniProtKB-KW oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3-oxo-5-beta-steroid 4-dehydrogenase activity Ref.2 Inferred from direct assay. Source: MGI delta4-3-oxosteroid 5beta-reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	3-oxo-5-beta-steroid 4-dehydrogenase		PRO_0000124672
Regions
	Nucleotide binding	168 – 169	2	NADP By similarity
	Nucleotide binding	220 – 224	5	NADP By similarity
	Nucleotide binding	273 – 283	11	NADP By similarity
Sites
	Active site	57	1	Proton donor By similarity
	Binding site	52	1	NADP By similarity
	Binding site	192	1	NADP By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	P31210-1 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 32D8266BFA62CE99	FASTA	326	37,378
	10 20 30 40 50 60 MNLSTANHHI PLNDGNSIPI IGLGTYSDPR PVPGKTFIAV KTAIDEGYRH IDGAYVYRNE 70 80 90 100 110 120 HEVGEAIREK VAEGKVKREE IFYCGKLWST DHDPEMVRPA LERTLQTLKL DYIDLYIIEM 130 140 150 160 170 180 PMAFKPGEEF YPKDENGRVI YHKSNLCATW EALEACKDAG LVKSLGVSNF NRRQLEVILN 190 200 210 220 230 240 KPGLKYKPVT NQVECHPYFT QTKLLEVSAS SMTSFIVAYS PLGTCRNPLW VNVSSPPLLK 250 260 270 280 290 300 DELLTSLGKK YNKTQAQIVL RFDIQRGLVV IPKSTTPERI KENFQIFDFS LTKEEMKDIE 310 320 ALNKNVRFVE MLMWSDHPEY PFHDEY

References

[1]	"Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver." Onishi Y., Noshiro M., Shimosato T., Okuda K. FEBS Lett. 283:215-218(1991) [PubMed: 1710579] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.
[2]	"Delta 4-3-Oxosteroid 5 beta-reductase. Structure and function." Onishi Y., Noshiro M., Shimosato T., Okuda K. Biol. Chem. Hoppe-Seyler 372:1039-1049(1991) [PubMed: 1789929] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Tissue: Liver.
[3]	"Identification of the 37-kd rat liver protein that forms an acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5 beta-reductase." Zhu Y., Fillenwarth M.J., Crabb D., Lumeng L., Lin R.C. Hepatology 23:115-122(1996) [PubMed: 8550030] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Liver.

Cross-references

Sequence databases
EMBLGenBankDDBJ	D17309 mRNA. Translation: BAA04131.1. S80431 mRNA. Translation: AAB35916.2.
IPI	IPI00210823.
PIR	S15835.
RefSeq	NP_620239.1.
UniGene	Rn.25716
3D structure databases
HSSP	HSSP built from PDB template 1LWI based on UniProtKB P23457.
ModBase	Search...
Proteomic databases
PRIDE	P31210.
Genome annotation databases
Ensembl	ENSRNOG00000013004. Rattus norvegicus. [Contig view]
GeneID	192242.
KEGG	rno:192242.
Organism-specific databases
RGD	620752. Akr1d1.
Phylogenomic databases
HOVERGEN	P31210.
Enzyme and pathway databases
BRENDA	1.3.1.3. 248.
Gene expression databases
ArrayExpress	P31210.
GermOnline	ENSRNOG00000013004. Rattus norvegicus.
Family and domain databases
InterPro	IPR001395. Aldo/ket_red. IPR018170. Aldo/ket_reductase_CS. [Graphical view]
Gene3D	G3DSA:3.20.20.100. Aldo/ket_red. 1 hit.
PANTHER	PTHR11732. Aldo/ket_red. 1 hit.
Pfam	PF00248. Aldo_ket_red. 1 hit. [Graphical view]
ProDom	PD000288. Aldo/ket_red. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00798. ALDOKETO_REDUCTASE_1. 1 hit. PS00062. ALDOKETO_REDUCTASE_2. 1 hit. PS00063. ALDOKETO_REDUCTASE_3. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	622866.

Entry information

Entry name

AK1D1_RAT

Accession

Primary (citable) accession number: P31210

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	June 16, 2009
This is version 72 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)