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Protein

3-oxo-5-beta-steroid 4-dehydrogenase

Gene

Akr1d1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.By similarity

Catalytic activityi

5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.By similarity
17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei26 – 261SubstrateBy similarity
Binding sitei52 – 521NADPBy similarity
Active sitei57 – 571Proton donorBy similarity
Binding sitei57 – 571SubstrateBy similarity
Binding sitei88 – 881SubstrateBy similarity
Binding sitei119 – 1191SubstrateBy similarity
Binding sitei131 – 1311SubstrateBy similarity
Binding sitei192 – 1921NADPBy similarity
Binding sitei230 – 2301SubstrateBy similarity

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi22 – 265NADPBy similarity
Nucleotide bindingi168 – 1692NADPBy similarity
Nucleotide bindingi219 – 2246NADPBy similarity
Nucleotide bindingi273 – 28311NADPBy similarityAdd
BLAST

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14304.
SABIO-RKP31210.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-beta-steroid 4-dehydrogenase (EC:1.3.1.3)
Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase
Gene namesi
Name:Akr1d1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620752. Akr1d1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry

ChEMBLiCHEMBL5760.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 3263263-oxo-5-beta-steroid 4-dehydrogenasePRO_0000124672Add
BLAST

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP31210.
PRIDEiP31210.

PTM databases

iPTMnetiP31210.
PhosphoSiteiP31210.

Interactioni

Protein-protein interaction databases

MINTiMINT-4564981.
STRINGi10116.ENSRNOP00000017675.

Structurei

3D structure databases

ProteinModelPortaliP31210.
SMRiP31210. Positions 2-326.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP31210.
KOiK00251.
PhylomeDBiP31210.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSTANHHI PLNDGNSIPI IGLGTYSDPR PVPGKTFIAV KTAIDEGYRH
60 70 80 90 100
IDGAYVYRNE HEVGEAIREK VAEGKVKREE IFYCGKLWST DHDPEMVRPA
110 120 130 140 150
LERTLQTLKL DYIDLYIIEM PMAFKPGEEF YPKDENGRVI YHKSNLCATW
160 170 180 190 200
EALEACKDAG LVKSLGVSNF NRRQLEVILN KPGLKYKPVT NQVECHPYFT
210 220 230 240 250
QTKLLEVSAS SMTSFIVAYS PLGTCRNPLW VNVSSPPLLK DELLTSLGKK
260 270 280 290 300
YNKTQAQIVL RFDIQRGLVV IPKSTTPERI KENFQIFDFS LTKEEMKDIE
310 320
ALNKNVRFVE MLMWSDHPEY PFHDEY
Length:326
Mass (Da):37,378
Last modified:July 1, 1993 - v1
Checksum:i32D8266BFA62CE99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17309 mRNA. Translation: BAA04131.1.
S80431 mRNA. Translation: AAB35916.2.
PIRiS15835.
RefSeqiNP_620239.1. NM_138884.1.
UniGeneiRn.25716.

Genome annotation databases

GeneIDi192242.
KEGGirno:192242.
UCSCiRGD:620752. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17309 mRNA. Translation: BAA04131.1.
S80431 mRNA. Translation: AAB35916.2.
PIRiS15835.
RefSeqiNP_620239.1. NM_138884.1.
UniGeneiRn.25716.

3D structure databases

ProteinModelPortaliP31210.
SMRiP31210. Positions 2-326.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4564981.
STRINGi10116.ENSRNOP00000017675.

Chemistry

ChEMBLiCHEMBL5760.

PTM databases

iPTMnetiP31210.
PhosphoSiteiP31210.

Proteomic databases

PaxDbiP31210.
PRIDEiP31210.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192242.
KEGGirno:192242.
UCSCiRGD:620752. rat.

Organism-specific databases

CTDi6718.
RGDi620752. Akr1d1.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP31210.
KOiK00251.
PhylomeDBiP31210.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14304.
SABIO-RKP31210.

Miscellaneous databases

PROiP31210.

Family and domain databases

Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and sequence analysis of cDNA encoding delta 4-3-ketosteroid 5 beta-reductase of rat liver."
    Onishi Y., Noshiro M., Shimosato T., Okuda K.
    FEBS Lett. 283:215-218(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.
  2. "Delta 4-3-Oxosteroid 5 beta-reductase. Structure and function."
    Onishi Y., Noshiro M., Shimosato T., Okuda K.
    Biol. Chem. Hoppe-Seyler 372:1039-1049(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Liver.
  3. "Identification of the 37-kd rat liver protein that forms an acetaldehyde adduct in vivo as delta 4-3-ketosteroid 5 beta-reductase."
    Zhu Y., Fillenwarth M.J., Crabb D., Lumeng L., Lin R.C.
    Hepatology 23:115-122(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Liver.

Entry informationi

Entry nameiAK1D1_RAT
AccessioniPrimary (citable) accession number: P31210
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: June 8, 2016
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.