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Protein

3-oxo-5-beta-steroid 4-dehydrogenase

Gene

Akr1d1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Efficiently catalyzes the reduction of progesterone, androstenedione, 17-alpha-hydroxyprogesterone and testosterone to 5-beta-reduced metabolites. The bile acid intermediates 7-alpha,12-alpha-dihydroxy-4-cholesten-3-one and 7-alpha-hydroxy-4-cholesten-3-one can also act as substrates.By similarity

Catalytic activityi

5-beta-cholestan-3-one + NADP+ = cholest-4-en-3-one + NADPH.By similarity
17,21-dihydroxy-5-beta-pregnane-3,11,20-trione + NADP+ = cortisone + NADPH.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei26SubstrateBy similarity1
Binding sitei52NADPBy similarity1
Active sitei57Proton donorBy similarity1
Binding sitei57SubstrateBy similarity1
Binding sitei88SubstrateBy similarity1
Binding sitei119SubstrateBy similarity1
Binding sitei131SubstrateBy similarity1
Binding sitei192NADPBy similarity1
Binding sitei230SubstrateBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi22 – 26NADPBy similarity5
Nucleotide bindingi168 – 169NADPBy similarity2
Nucleotide bindingi219 – 224NADPBy similarity6
Nucleotide bindingi273 – 283NADPBy similarityAdd BLAST11

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Oxidoreductase

Keywords - Biological processi

Bile acid catabolism, Lipid degradation, Lipid metabolism, Steroid metabolism

Keywords - Ligandi

NADP

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14304.
SABIO-RKP31210.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxo-5-beta-steroid 4-dehydrogenase (EC:1.3.1.3)
Alternative name(s):
Aldo-keto reductase family 1 member D1
Delta(4)-3-ketosteroid 5-beta-reductase
Delta(4)-3-oxosteroid 5-beta-reductase
Gene namesi
Name:Akr1d1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi620752. Akr1d1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL5760.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001246721 – 3263-oxo-5-beta-steroid 4-dehydrogenaseAdd BLAST326

Post-translational modificationi

The N-terminus is blocked.

Proteomic databases

PaxDbiP31210.
PRIDEiP31210.

PTM databases

iPTMnetiP31210.
PhosphoSitePlusiP31210.

Interactioni

Protein-protein interaction databases

MINTiMINT-4564981.
STRINGi10116.ENSRNOP00000017675.

Structurei

3D structure databases

ProteinModelPortaliP31210.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP31210.
KOiK00251.
PhylomeDBiP31210.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31210-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MNLSTANHHI PLNDGNSIPI IGLGTYSDPR PVPGKTFIAV KTAIDEGYRH
60 70 80 90 100
IDGAYVYRNE HEVGEAIREK VAEGKVKREE IFYCGKLWST DHDPEMVRPA
110 120 130 140 150
LERTLQTLKL DYIDLYIIEM PMAFKPGEEF YPKDENGRVI YHKSNLCATW
160 170 180 190 200
EALEACKDAG LVKSLGVSNF NRRQLEVILN KPGLKYKPVT NQVECHPYFT
210 220 230 240 250
QTKLLEVSAS SMTSFIVAYS PLGTCRNPLW VNVSSPPLLK DELLTSLGKK
260 270 280 290 300
YNKTQAQIVL RFDIQRGLVV IPKSTTPERI KENFQIFDFS LTKEEMKDIE
310 320
ALNKNVRFVE MLMWSDHPEY PFHDEY
Length:326
Mass (Da):37,378
Last modified:July 1, 1993 - v1
Checksum:i32D8266BFA62CE99
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17309 mRNA. Translation: BAA04131.1.
S80431 mRNA. Translation: AAB35916.2.
PIRiS15835.
RefSeqiNP_620239.1. NM_138884.1.
UniGeneiRn.25716.

Genome annotation databases

GeneIDi192242.
KEGGirno:192242.
UCSCiRGD:620752. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D17309 mRNA. Translation: BAA04131.1.
S80431 mRNA. Translation: AAB35916.2.
PIRiS15835.
RefSeqiNP_620239.1. NM_138884.1.
UniGeneiRn.25716.

3D structure databases

ProteinModelPortaliP31210.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-4564981.
STRINGi10116.ENSRNOP00000017675.

Chemistry databases

ChEMBLiCHEMBL5760.

PTM databases

iPTMnetiP31210.
PhosphoSitePlusiP31210.

Proteomic databases

PaxDbiP31210.
PRIDEiP31210.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi192242.
KEGGirno:192242.
UCSCiRGD:620752. rat.

Organism-specific databases

CTDi6718.
RGDi620752. Akr1d1.

Phylogenomic databases

eggNOGiKOG1577. Eukaryota.
COG0656. LUCA.
HOGENOMiHOG000250272.
HOVERGENiHBG000020.
InParanoidiP31210.
KOiK00251.
PhylomeDBiP31210.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-14304.
SABIO-RKP31210.

Miscellaneous databases

PROiP31210.

Family and domain databases

CDDicd06660. Aldo_ket_red. 1 hit.
Gene3Di3.20.20.100. 1 hit.
InterProiIPR001395. Aldo/ket_red/Kv-b.
IPR018170. Aldo/ket_reductase_CS.
IPR020471. Aldo/keto_reductase.
IPR023210. NADP_OxRdtase_dom.
[Graphical view]
PANTHERiPTHR11732. PTHR11732. 2 hits.
PfamiPF00248. Aldo_ket_red. 1 hit.
[Graphical view]
PIRSFiPIRSF000097. AKR. 1 hit.
PRINTSiPR00069. ALDKETRDTASE.
SUPFAMiSSF51430. SSF51430. 1 hit.
PROSITEiPS00798. ALDOKETO_REDUCTASE_1. 1 hit.
PS00062. ALDOKETO_REDUCTASE_2. 1 hit.
PS00063. ALDOKETO_REDUCTASE_3. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAK1D1_RAT
AccessioniPrimary (citable) accession number: P31210
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.