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Protein

Polyadenylate-binding protein, cytoplasmic and nuclear

Gene

pab1

Organism
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds the poly(A) tail of mRNA. Appears to be an important mediator of the multiple roles of the poly(A) tail in mRNA biogenesis, stability and translation. In the nucleus, involved in both mRNA cleavage and polyadenylation. Is also required for efficient mRNA export to the cytoplasm. Acts in concert with a poly(A)-specific nuclease (PAN) to affect poly(A) tail shortening, which may occur concomitantly with either nucleocytoplasmic mRNA transport or translational initiation. In the cytoplasm, stimulates translation initiation and regulates mRNA decay through translation termination-coupled poly(A) shortening, probably mediated by PAN (By similarity).By similarity1 Publication

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) binding Source: PomBase

GO - Biological processi

  • mRNA export from nucleus Source: PomBase
  • mRNA processing Source: UniProtKB-KW
  • regulation of translation Source: UniProtKB-KW
Complete GO annotation...

Keywords - Biological processi

mRNA processing, mRNA transport, Translation regulation, Transport

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-429947. Deadenylation of mRNA.
R-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Names & Taxonomyi

Protein namesi
Recommended name:
Polyadenylate-binding protein, cytoplasmic and nuclear
Short name:
PABP
Short name:
Poly(A)-binding protein
Alternative name(s):
Polyadenylate tail-binding protein
Gene namesi
Name:pab1
Synonyms:pabp
ORF Names:SPAC57A7.04c
OrganismiSchizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Taxonomic identifieri284812 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaTaphrinomycotinaSchizosaccharomycetesSchizosaccharomycetalesSchizosaccharomycetaceaeSchizosaccharomyces
Proteomesi
  • UP000002485 Componenti: Chromosome I

Organism-specific databases

EuPathDBiFungiDB:SPAC57A7.04c.
PomBaseiSPAC57A7.04c.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: PomBase
  • cytoplasmic stress granule Source: PomBase
  • cytosol Source: PomBase
  • nucleus Source: PomBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 653653Polyadenylate-binding protein, cytoplasmic and nuclearPRO_0000081718Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei167 – 1671Phosphothreonine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP31209.
PRIDEiP31209.

PTM databases

iPTMnetiP31209.

Interactioni

Subunit structurei

Interacts with cid13.1 Publication

Protein-protein interaction databases

BioGridi278007. 13 interactions.
IntActiP31209. 1 interaction.
MINTiMINT-472058.

Structurei

3D structure databases

ProteinModelPortaliP31209.
SMRiP31209. Positions 80-249, 356-447.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini80 – 15879RRM 1PROSITE-ProRule annotationAdd
BLAST
Domaini168 – 24578RRM 2PROSITE-ProRule annotationAdd
BLAST
Domaini261 – 33878RRM 3PROSITE-ProRule annotationAdd
BLAST
Domaini364 – 44178RRM 4PROSITE-ProRule annotationAdd
BLAST
Domaini569 – 64678PABCPROSITE-ProRule annotationAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi481 – 56989Pro-richAdd
BLAST

Sequence similaritiesi

Contains 1 PABC domain.PROSITE-ProRule annotation
Contains 4 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

HOGENOMiHOG000217922.
InParanoidiP31209.
KOiK13126.
OMAiANHECAQ.
OrthoDBiEOG706125.
PhylomeDBiP31209.

Family and domain databases

Gene3Di1.10.1900.10. 1 hit.
3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR006515. PABP_1234.
IPR002004. PABP_HYD.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00658. PABP. 1 hit.
PF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00517. PolyA. 1 hit.
SM00360. RRM. 4 hits.
SM00361. RRM_1. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
SSF63570. SSF63570. 1 hit.
TIGRFAMsiTIGR01628. PABP-1234. 1 hit.
PROSITEiPS51309. PABC. 1 hit.
PS50102. RRM. 4 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31209-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MPSTDLKKQA DAAVESDVNT NNEAVESSTK EESSNTPSTE TQPEKKAEEP
60 70 80 90 100
EAAAEPSEST STPTNASSVA TPSGTAPTSA SLYVGELDPS VTEAMLFELF
110 120 130 140 150
NSIGPVASIR VCRDAVTRRS LGYAYVNFHN MEDGEKALDE LNYTLIKGRP
160 170 180 190 200
CRIMWSQRDP SLRKMGTGNV FIKNLDPAID NKALHDTFSA FGKILSCKVA
210 220 230 240 250
VDELGNAKGY GFVHFDSVES ANAAIEHVNG MLLNDKKVYV GHHVSRRERQ
260 270 280 290 300
SKVEALKANF TNVYIKNLDT EITEQEFSDL FGQFGEITSL SLVKDQNDKP
310 320 330 340 350
RGFGFVNYAN HECAQKAVDE LNDKEYKGKK LYVGRAQKKH EREEELRKRY
360 370 380 390 400
EQMKLEKMNK YQGVNLFIKN LQDEVDDERL KAEFSAFGTI TSAKIMTDEQ
410 420 430 440 450
GKSKGFGFVC YTTPEEANKA VTEMNQRMLA GKPLYVALAQ RKEVRRSQLE
460 470 480 490 500
AQIQARNQFR LQQQVAAAAG IPAVQYGATG PLIYGPGGYP IPAAVNGRGM
510 520 530 540 550
PMVPGHNGPM PMYPGMPTQF PAGGPAPGYP GMNARGPVPA QGRPMMMPGS
560 570 580 590 600
VPSAGPAEAE AVPAVPGMPE RFTAADLAAV PEESRKQVLG ELLYPKVFVR
610 620 630 640 650
EEKLSGKITG MLLEMPNSEL LELLEDDSAL NERVNEAIGV LQEFVDQEPG

FTE
Length:653
Mass (Da):71,513
Last modified:July 15, 1998 - v2
Checksum:i7F8F5CAD69D0CFE1
GO

Sequence cautioni

The sequence AAA35320.1 differs from that shown. Reason: Frameshift at positions 518 and 526. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 3434MPSTD…KEESS → MSLENSSTLSLCSNNTTHFW in AAA35320 (PubMed:1675426).CuratedAdd
BLAST
Sequence conflicti349 – 3491R → A in AAA35320 (PubMed:1675426).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB08762.1.
M64603 mRNA. Translation: AAA35320.1. Frameshift.
PIRiT38950. DNZPPA.
RefSeqiNP_593377.1. NM_001018809.2.

Genome annotation databases

EnsemblFungiiSPAC57A7.04c.1; SPAC57A7.04c.1:pep; SPAC57A7.04c.
GeneIDi2541505.
KEGGispo:SPAC57A7.04c.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CU329670 Genomic DNA. Translation: CAB08762.1.
M64603 mRNA. Translation: AAA35320.1. Frameshift.
PIRiT38950. DNZPPA.
RefSeqiNP_593377.1. NM_001018809.2.

3D structure databases

ProteinModelPortaliP31209.
SMRiP31209. Positions 80-249, 356-447.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi278007. 13 interactions.
IntActiP31209. 1 interaction.
MINTiMINT-472058.

PTM databases

iPTMnetiP31209.

Proteomic databases

MaxQBiP31209.
PRIDEiP31209.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiSPAC57A7.04c.1; SPAC57A7.04c.1:pep; SPAC57A7.04c.
GeneIDi2541505.
KEGGispo:SPAC57A7.04c.

Organism-specific databases

EuPathDBiFungiDB:SPAC57A7.04c.
PomBaseiSPAC57A7.04c.

Phylogenomic databases

HOGENOMiHOG000217922.
InParanoidiP31209.
KOiK13126.
OMAiANHECAQ.
OrthoDBiEOG706125.
PhylomeDBiP31209.

Enzyme and pathway databases

ReactomeiR-SPO-156827. L13a-mediated translational silencing of Ceruloplasmin expression.
R-SPO-429947. Deadenylation of mRNA.
R-SPO-975956. Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC).
R-SPO-975957. Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC).

Miscellaneous databases

NextBioi20802604.
PROiP31209.

Family and domain databases

Gene3Di1.10.1900.10. 1 hit.
3.30.70.330. 4 hits.
InterProiIPR012677. Nucleotide-bd_a/b_plait.
IPR006515. PABP_1234.
IPR002004. PABP_HYD.
IPR000504. RRM_dom.
IPR003954. RRM_dom_euk.
[Graphical view]
PfamiPF00658. PABP. 1 hit.
PF00076. RRM_1. 4 hits.
[Graphical view]
SMARTiSM00517. PolyA. 1 hit.
SM00360. RRM. 4 hits.
SM00361. RRM_1. 4 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 2 hits.
SSF63570. SSF63570. 1 hit.
TIGRFAMsiTIGR01628. PABP-1234. 1 hit.
PROSITEiPS51309. PABC. 1 hit.
PS50102. RRM. 4 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "The genome sequence of Schizosaccharomyces pombe."
    Wood V., Gwilliam R., Rajandream M.A., Lyne M.H., Lyne R., Stewart A., Sgouros J.G., Peat N., Hayles J., Baker S.G., Basham D., Bowman S., Brooks K., Brown D., Brown S., Chillingworth T., Churcher C.M., Collins M.
    , Connor R., Cronin A., Davis P., Feltwell T., Fraser A., Gentles S., Goble A., Hamlin N., Harris D.E., Hidalgo J., Hodgson G., Holroyd S., Hornsby T., Howarth S., Huckle E.J., Hunt S., Jagels K., James K.D., Jones L., Jones M., Leather S., McDonald S., McLean J., Mooney P., Moule S., Mungall K.L., Murphy L.D., Niblett D., Odell C., Oliver K., O'Neil S., Pearson D., Quail M.A., Rabbinowitsch E., Rutherford K.M., Rutter S., Saunders D., Seeger K., Sharp S., Skelton J., Simmonds M.N., Squares R., Squares S., Stevens K., Taylor K., Taylor R.G., Tivey A., Walsh S.V., Warren T., Whitehead S., Woodward J.R., Volckaert G., Aert R., Robben J., Grymonprez B., Weltjens I., Vanstreels E., Rieger M., Schaefer M., Mueller-Auer S., Gabel C., Fuchs M., Duesterhoeft A., Fritzc C., Holzer E., Moestl D., Hilbert H., Borzym K., Langer I., Beck A., Lehrach H., Reinhardt R., Pohl T.M., Eger P., Zimmermann W., Wedler H., Wambutt R., Purnelle B., Goffeau A., Cadieu E., Dreano S., Gloux S., Lelaure V., Mottier S., Galibert F., Aves S.J., Xiang Z., Hunt C., Moore K., Hurst S.M., Lucas M., Rochet M., Gaillardin C., Tallada V.A., Garzon A., Thode G., Daga R.R., Cruzado L., Jimenez J., Sanchez M., del Rey F., Benito J., Dominguez A., Revuelta J.L., Moreno S., Armstrong J., Forsburg S.L., Cerutti L., Lowe T., McCombie W.R., Paulsen I., Potashkin J., Shpakovski G.V., Ussery D., Barrell B.G., Nurse P.
    Nature 415:871-880(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 972 / ATCC 24843.
  2. "The multiple RNA-binding domains of the mRNA poly(A)-binding protein have different RNA-binding activities."
    Burd C.G., Matunis E.L., Dreyfuss G.
    Mol. Cell. Biol. 11:3419-3424(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 1-628.
  3. "Cid13 is a cytoplasmic poly(A) polymerase that regulates ribonucleotide reductase mRNA."
    Saitoh S., Chabes A., McDonald W.H., Thelander L., Yates J.R. III, Russell P.
    Cell 109:563-573(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH CID13.
  4. "Schizosaccharomyces pombe spPABP, a homologue of Saccharomyces cerevisiae Pab1p, is a non-essential, shuttling protein that facilitates mRNA export."
    Thakurta A.G., Ho Yoon J., Dhar R.
    Yeast 19:803-810(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION IN MRNA EXPORT, SUBCELLULAR LOCATION.
  5. "ORFeome cloning and global analysis of protein localization in the fission yeast Schizosaccharomyces pombe."
    Matsuyama A., Arai R., Yashiroda Y., Shirai A., Kamata A., Sekido S., Kobayashi Y., Hashimoto A., Hamamoto M., Hiraoka Y., Horinouchi S., Yoshida M.
    Nat. Biotechnol. 24:841-847(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  6. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT THR-167, IDENTIFICATION BY MASS SPECTROMETRY.

Entry informationi

Entry nameiPABP_SCHPO
AccessioniPrimary (citable) accession number: P31209
Secondary accession number(s): P87135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 15, 1998
Last modified: May 11, 2016
This is version 133 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Schizosaccharomyces pombe
    Schizosaccharomyces pombe: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.