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P31206

- NANH_BACFR

UniProt

P31206 - NANH_BACFR

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Protein

Sialidase

Gene

nanH

Organism
Bacteroides fragilis (strain YCH46)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi

Functioni

Sialidases have been suggested to be pathogenic factors in microbial infections.

Catalytic activityi

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei399 – 3991Sequence Analysis
Binding sitei415 – 4151SubstrateBy similarity
Binding sitei479 – 4791SubstrateBy similarity

GO - Molecular functioni

  1. exo-alpha-(2->3)-sialidase activity Source: UniProtKB-EC
  2. exo-alpha-(2->6)-sialidase activity Source: UniProtKB-EC
  3. exo-alpha-(2->8)-sialidase activity Source: UniProtKB-EC

GO - Biological processi

  1. pathogenesis Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Protein family/group databases

CAZyiGH33. Glycoside Hydrolase Family 33.

Names & Taxonomyi

Protein namesi
Recommended name:
Sialidase (EC:3.2.1.18)
Alternative name(s):
Neuraminidase
Gene namesi
Name:nanH
Ordered Locus Names:BF1729
OrganismiBacteroides fragilis (strain YCH46)
Taxonomic identifieri295405 [NCBI]
Taxonomic lineageiBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides
ProteomesiUP000002197: Chromosome

Subcellular locationi

GO - Cellular componenti

  1. periplasmic space Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 2222Sequence AnalysisAdd
BLAST
Chaini23 – 544522SialidasePRO_0000012029Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi295405.BF1729.

Structurei

3D structure databases

ProteinModelPortaliP31206.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati239 – 25012BNR 1Add
BLAST
Repeati318 – 32912BNR 2Add
BLAST
Repeati378 – 38912BNR 3Add
BLAST
Repeati425 – 43612BNR 4Add
BLAST
Repeati485 – 49612BNR 5Add
BLAST

Sequence similaritiesi

Belongs to the glycosyl hydrolase 33 family.Curated
Contains 5 BNR repeats.Curated

Keywords - Domaini

Repeat, Signal

Phylogenomic databases

eggNOGiCOG4409.
HOGENOMiHOG000252336.
KOiK01186.
OMAiINITEQV.
OrthoDBiEOG67DPK3.

Family and domain databases

Gene3Di2.120.10.10. 1 hit.
InterProiIPR002860. BNR_rpt.
IPR026856. Sialidase_fam.
IPR029456. Sialidase_N.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERiPTHR10628. PTHR10628. 1 hit.
PfamiPF02012. BNR. 3 hits.
PF14873. BNR_assoc_N. 1 hit.
[Graphical view]
PRINTSiPR01803. TCSIALIDASE.
SUPFAMiSSF50939. SSF50939. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31206-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKKAVILFSL FCFLCAIPVV QAADTIFVRE TRIPILIERQ DNVLFYLRLD
60 70 80 90 100
AKESQTLNDV VLNLGEGVNL SEIQSIKLYY GGTEALQDSG KKRFAPVGYI
110 120 130 140 150
SSNTPGKTLA ANPSYSIKKS EVTNPGNQVV LKGDQKLFPG INYFWISLQM
160 170 180 190 200
KPGTSLTSKV TADIASITLD GKKALLDVVS ENGIEHRMGV GVRHAGDDNS
210 220 230 240 250
AAFRIPGLVT TNKGTLLGVY DVRYNSSVDL QEHVDVGLSR STDGGKTWEK
260 270 280 290 300
MRLPLAFGEF GGLPAGQNGV GDPSILVDTK TNNVWVVAAW THGMGNQRAW
310 320 330 340 350
WSSHPGMDMN HTAQLVLAKS TDDGKTWSAP INITEQVKDP SWYFLLQGPG
360 370 380 390 400
RGITMSDGTL VFPTQFIDST RVPNAGIMYS KDGGKNWKMH NYARTNTTEA
410 420 430 440 450
QVAEVEPGVL MLNMRDNRGG SRAVAITKDL GKTWTEHESS RKALPESVCM
460 470 480 490 500
ASLISVKAKD NVLGKDLLIF SNPNTTKGRY NTTIKISLDG GVTWSPEHQL
510 520 530 540
LLDEGNNWGY SCLSMIDKET IGILYESSVA HMTFQAVKLK DIIK
Length:544
Mass (Da):59,556
Last modified:November 23, 2004 - v2
Checksum:i6D1D185AB15FA822
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti197 – 1971D → A in BAA05853. (PubMed:8093075)Curated
Sequence conflicti405 – 4051V → A in AAA22912. (PubMed:2158974)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28493 Genomic DNA. Translation: BAA05853.1.
AB102772 Genomic DNA. Translation: BAC56895.2.
AP006841 Genomic DNA. Translation: BAD48476.1.
M31663 Genomic DNA. Translation: AAA22912.1.
PIRiJC2500.
RefSeqiYP_099010.1. NC_006347.1.

Genome annotation databases

EnsemblBacteriaiBAD48476; BAD48476; BF1729.
GeneIDi3082956.
KEGGibfr:BF1729.
PATRICi21049123. VBIBacFra17906_1678.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
D28493 Genomic DNA. Translation: BAA05853.1 .
AB102772 Genomic DNA. Translation: BAC56895.2 .
AP006841 Genomic DNA. Translation: BAD48476.1 .
M31663 Genomic DNA. Translation: AAA22912.1 .
PIRi JC2500.
RefSeqi YP_099010.1. NC_006347.1.

3D structure databases

ProteinModelPortali P31206.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 295405.BF1729.

Protein family/group databases

CAZyi GH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai BAD48476 ; BAD48476 ; BF1729 .
GeneIDi 3082956.
KEGGi bfr:BF1729.
PATRICi 21049123. VBIBacFra17906_1678.

Phylogenomic databases

eggNOGi COG4409.
HOGENOMi HOG000252336.
KOi K01186.
OMAi INITEQV.
OrthoDBi EOG67DPK3.

Family and domain databases

Gene3Di 2.120.10.10. 1 hit.
InterProi IPR002860. BNR_rpt.
IPR026856. Sialidase_fam.
IPR029456. Sialidase_N.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view ]
PANTHERi PTHR10628. PTHR10628. 1 hit.
Pfami PF02012. BNR. 3 hits.
PF14873. BNR_assoc_N. 1 hit.
[Graphical view ]
PRINTSi PR01803. TCSIALIDASE.
SUPFAMi SSF50939. SSF50939. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequence of the Bacteroides fragilis YCH46 neuraminidase-encoding gene."
    Akimoto S., Ono T., Tsutsui H., Kinouchi T., Kataoka K., Ohnishi Y.
    Biochem. Biophys. Res. Commun. 203:914-921(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YCH46.
  2. "Characterization of a gene cluster for degradation of sialoglycoconjugates in Bacteroides fragilis strain YCH46."
    Nakayama H., Kuwahara T., Iwasa T., Okamoto S., Tsuchihashi Y., Nakanishi K., Kataoka K., Arimochi H., Ohnishi Y.
    Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: YCH46.
  3. "Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."
    Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.
    Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: YCH46.
  4. "Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli."
    Russo T.A., Thompson J.S., Godoy V.G., Malamy M.H.
    J. Bacteriol. 172:2594-2600(1990) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 235-500.
    Strain: TAL2480.

Entry informationi

Entry nameiNANH_BACFR
AccessioniPrimary (citable) accession number: P31206
Secondary accession number(s): Q45145, Q64VK0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 23, 2004
Last modified: October 29, 2014
This is version 98 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3