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P31206 (NANH_BACFR) Reviewed, UniProtKB/Swiss-Prot

Last modified November 13, 2013. Version 94. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sialidase

EC=3.2.1.18
Alternative name(s):
Neuraminidase
Gene names
Name:nanH
Ordered Locus Names:BF1729
OrganismBacteroides fragilis (strain YCH46) [Complete proteome] [HAMAP]
Taxonomic identifier295405 [NCBI]
Taxonomic lineageBacteriaBacteroidetesBacteroidiaBacteroidalesBacteroidaceaeBacteroides

Protein attributes

Sequence length544 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceInferred from homology

General annotation (Comments)

Function

Sialidases have been suggested to be pathogenic factors in microbial infections.

Catalytic activity

Hydrolysis of alpha-(2->3)-, alpha-(2->6)-, alpha-(2->8)- glycosidic linkages of terminal sialic acid residues in oligosaccharides, glycoproteins, glycolipids, colominic acid and synthetic substrates.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the glycosyl hydrolase 33 family.

Contains 5 BNR repeats.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainRepeat
Signal
   Molecular functionGlycosidase
Hydrolase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processpathogenesis

Inferred from electronic annotation. Source: InterPro

   Cellular_componentperiplasmic space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionexo-alpha-(2->3)-sialidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

exo-alpha-(2->6)-sialidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

exo-alpha-(2->8)-sialidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2222 Potential
Chain23 – 544522Sialidase
PRO_0000012029

Regions

Repeat239 – 25012BNR 1
Repeat318 – 32912BNR 2
Repeat378 – 38912BNR 3
Repeat425 – 43612BNR 4
Repeat485 – 49612BNR 5

Sites

Active site3991 Potential
Binding site4151Substrate By similarity
Binding site4791Substrate By similarity

Experimental info

Sequence conflict1971D → A in BAA05853. Ref.1
Sequence conflict4051V → A in AAA22912. Ref.4

Sequences

Sequence LengthMass (Da)Tools
P31206 [UniParc].

Last modified November 23, 2004. Version 2.
Checksum: 6D1D185AB15FA822

FASTA54459,556
        10         20         30         40         50         60 
MKKAVILFSL FCFLCAIPVV QAADTIFVRE TRIPILIERQ DNVLFYLRLD AKESQTLNDV 

        70         80         90        100        110        120 
VLNLGEGVNL SEIQSIKLYY GGTEALQDSG KKRFAPVGYI SSNTPGKTLA ANPSYSIKKS 

       130        140        150        160        170        180 
EVTNPGNQVV LKGDQKLFPG INYFWISLQM KPGTSLTSKV TADIASITLD GKKALLDVVS 

       190        200        210        220        230        240 
ENGIEHRMGV GVRHAGDDNS AAFRIPGLVT TNKGTLLGVY DVRYNSSVDL QEHVDVGLSR 

       250        260        270        280        290        300 
STDGGKTWEK MRLPLAFGEF GGLPAGQNGV GDPSILVDTK TNNVWVVAAW THGMGNQRAW 

       310        320        330        340        350        360 
WSSHPGMDMN HTAQLVLAKS TDDGKTWSAP INITEQVKDP SWYFLLQGPG RGITMSDGTL 

       370        380        390        400        410        420 
VFPTQFIDST RVPNAGIMYS KDGGKNWKMH NYARTNTTEA QVAEVEPGVL MLNMRDNRGG 

       430        440        450        460        470        480 
SRAVAITKDL GKTWTEHESS RKALPESVCM ASLISVKAKD NVLGKDLLIF SNPNTTKGRY 

       490        500        510        520        530        540 
NTTIKISLDG GVTWSPEHQL LLDEGNNWGY SCLSMIDKET IGILYESSVA HMTFQAVKLK 


DIIK 

« Hide

References

« Hide 'large scale' references
[1]"Complete sequence of the Bacteroides fragilis YCH46 neuraminidase-encoding gene."
Akimoto S., Ono T., Tsutsui H., Kinouchi T., Kataoka K., Ohnishi Y.
Biochem. Biophys. Res. Commun. 203:914-921(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YCH46.
[2]"Characterization of a gene cluster for degradation of sialoglycoconjugates in Bacteroides fragilis strain YCH46."
Nakayama H., Kuwahara T., Iwasa T., Okamoto S., Tsuchihashi Y., Nakanishi K., Kataoka K., Arimochi H., Ohnishi Y.
Submitted (JAN-2005) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YCH46.
[3]"Genomic analysis of Bacteroides fragilis reveals extensive DNA inversions regulating cell surface adaptation."
Kuwahara T., Yamashita A., Hirakawa H., Nakayama H., Toh H., Okada N., Kuhara S., Hattori M., Hayashi T., Ohnishi Y.
Proc. Natl. Acad. Sci. U.S.A. 101:14919-14924(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: YCH46.
[4]"Cloning and expression of the Bacteroides fragilis TAL2480 neuraminidase gene, nanH, in Escherichia coli."
Russo T.A., Thompson J.S., Godoy V.G., Malamy M.H.
J. Bacteriol. 172:2594-2600(1990) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 235-500.
Strain: TAL2480.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D28493 Genomic DNA. Translation: BAA05853.1.
AB102772 Genomic DNA. Translation: BAC56895.2.
AP006841 Genomic DNA. Translation: BAD48476.1.
M31663 Genomic DNA. Translation: AAA22912.1.
PIRJC2500.
RefSeqYP_099010.1. NC_006347.1.

3D structure databases

ProteinModelPortalP31206.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING295405.BF1729.

Protein family/group databases

CAZyGH33. Glycoside Hydrolase Family 33.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAD48476; BAD48476; BF1729.
GeneID3082956.
KEGGbfr:BF1729.
PATRIC21049123. VBIBacFra17906_1678.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG4409.
HOGENOMHOG000252336.
KOK01186.
OMAINITEQV.
OrthoDBEOG67DPK3.
ProtClustDBCLSK822190.

Family and domain databases

Gene3D2.120.10.10. 1 hit.
InterProIPR002860. BNR_rpt.
IPR026948. Sialidase/anhydrosialidase.
IPR026856. Sialidase_fam.
IPR008377. Sialidase_trypan.
IPR011040. Sialidases.
[Graphical view]
PANTHERPTHR10628. PTHR10628. 1 hit.
PTHR10628:SF5. PTHR10628:SF5. 1 hit.
PfamPF02012. BNR. 3 hits.
[Graphical view]
PRINTSPR01803. TCSIALIDASE.
SUPFAMSSF50939. SSF50939. 1 hit.
ProtoNetSearch...

Entry information

Entry nameNANH_BACFR
AccessionPrimary (citable) accession number: P31206
Secondary accession number(s): Q45145, Q64VK0
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 23, 2004
Last modified: November 13, 2013
This is version 94 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries