Reviewed, UniProtKB/Swiss-Prot P31166 (APT1_ARATH)

Names and origin

Protein names

Recommended name:
    Adenine phosphoribosyltransferase 1
      Short name=APRT 1
    EC=2.4.2.7

Gene names

Name:	APT1
Synonyms:	APT
Ordered Locus Names:	At1g27450
ORF Names:	F17L21.24

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes a salvage reaction resulting in the formation of AMP, that is energically less costly than de novo synthesis.
Catalytic activity	AMP + diphosphate = adenine + 5-phospho-alpha-D-ribose 1-diphosphate.
Pathway	Purine metabolism; AMP biosynthesis via salvage pathway; AMP from adenine: step 1/1.
Subunit structure	Homodimer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the purine/pyrimidine phosphoribosyltransferase family.
Sequence caution	The sequence AAF99737.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Purine salvage
Cellular component	Cytoplasm
Coding sequence diversity	Alternative splicing
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	adenine salvage Traceable author statement. Source: TAIR purine ribonucleoside salvage Inferred from electronic annotation. Source: UniProtKB-KW response to cadmium ion Inferred from expression pattern. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR cytosol Inferred from direct assay. Source: TAIR plant-type cell wall Inferred from direct assay. Source: TAIR plasma membrane Inferred from direct assay. Source: TAIR
Molecular function	adenine phosphoribosyltransferase activity Inferred from direct assay. Source: TAIR
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P31166-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Note: No experimental confirmation available.

Isoform 2 (identifier: P31166-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-60: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	Adenine phosphoribosyltransferase 1		PRO_0000149514
Natural variations
	Alternative sequence	1 – 60	60	Missing in isoform 2.		VSP_009002

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 21, 2003. Version 2. Checksum: 92ABF089CA66C0FD	FASTA	243	26,396
	10 20 30 40 50 60 MQTIIISPLV SHRLCLARAV PCNRLLNNHH RAPPSIRLSN HRSTTSLRLF SSAAASRDSE 70 80 90 100 110 120 MATEDVQDPR IAKIASSIRV IPDFPKPGIM FQDITTLLLD TEAFKDTIAL FVDRYKDKGI 130 140 150 160 170 180 SVVAGVEARG FIFGPPIALA IGAKFVPMRK PKKLPGKVIS EEYSLEYGTD TIEMHVGAVE 190 200 210 220 230 240 PGERAIIIDD LIATGGTLAA AIRLLERVGV KIVECACVIE LPELKGKEKL GETSLFVLVK SAA
	Isoform 2. Checksum: A9D5FDF7FD130254	FASTA	183	19,739
	10 20 30 40 50 60 MATEDVQDPR IAKIASSIRV IPDFPKPGIM FQDITTLLLD TEAFKDTIAL FVDRYKDKGI 70 80 90 100 110 120 SVVAGVEARG FIFGPPIALA IGAKFVPMRK PKKLPGKVIS EEYSLEYGTD TIEMHVGAVE 130 140 150 160 170 180 PGERAIIIDD LIATGGTLAA AIRLLERVGV KIVECACVIE LPELKGKEKL GETSLFVLVK SAA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A complete cDNA for adenine phosphoribosyltransferase from Arabidopsis thaliana." Moffatt B.A., McWhinnie E.A., Burkhart W.E., Pasternak J.J., Rothstein S.J. Plant Mol. Biol. 18:653-662(1992) [PubMed: 1558943] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Strain: cv. Columbia.
[2]	"The adenine phosphoribosyltransferase-encoding gene of Arabidopsis thaliana." Moffatt B.A., McWhinnie E.A., Agarwal S.K., Schaff D.A. Gene 143:211-216(1994) [PubMed: 8206375] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 2). Strain: cv. Columbia.
[3]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2). Strain: cv. Columbia.
[5]	"Full-length cDNA from Arabidopsis thaliana." Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A. Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X58640 mRNA. Translation: CAA41497.1. L19637 Genomic DNA. Translation: AAA20677.1. AC004557 Genomic DNA. Translation: AAF99737.1. Sequence problems. AF325045 mRNA. Translation: AAG40397.1. AY128377 mRNA. Translation: AAM91580.1. BT000370 mRNA. Translation: AAN15689.1. AY084300 mRNA. Translation: AAM60891.1.
IPI	IPI00517864. IPI00542898.
PIR	G86399. S20867.
RefSeq	NP_564284.1. NP_849714.1.
UniGene	At.25013 Rra.8977
3D structure databases
ModBase	Search...
Protein-protein interaction databases
IntAct	P31166. 1 interaction.
STRING	P31166.
Proteomic databases
PRIDE	P31166.
Genome annotation databases
GeneID	839636.
GenomeReviews	Gene locus AT1G27450 in contig CT485782_GR.
KEGG	ath:AT1G27450.
NMPDR	fig|3702.1.peg.3072.
Organism-specific databases
TAIR	At1g27450.
Phylogenomic databases
eggNOG	KOG1712.
HOGENOM	HBG703830.
InParanoid	P31166.
OMA	VIPDFPK.
PhylomeDB	P31166.
Enzyme and pathway databases
BRENDA	2.4.2.7. 302.
Gene expression databases
ArrayExpress	P31166.
Genevestigator	P31166.
GermOnline	AT1G27450. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR005764. Ade_phspho_trans. IPR000836. PRibTrfase. [Graphical view]
Pfam	PF00156. Pribosyltran. 1 hit. [Graphical view]
TIGRFAMs	TIGR01090. apt. 1 hit.
PROSITE	PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

APT1_ARATH

Accession

Primary (citable) accession number: P31166
Secondary accession number(s): Q8LGF6, Q9FZJ0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 21, 2003
Last modified:	February 9, 2010
This is version 82 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)