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Protein

Putrescine transport system permease protein PotH

Gene

potH

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine.

GO - Molecular functioni

  • putrescine-importing ATPase activity Source: EcoCyc

GO - Biological processi

  • putrescine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTH-MONOMER.
ECOL316407:JW0840-MONOMER.
MetaCyc:POTH-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.2. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine transport system permease protein PotH
Gene namesi
Name:potH
Ordered Locus Names:b0856, JW0840
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11631. potH.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 3131CytoplasmicSequence analysisAdd
BLAST
Transmembranei32 – 5120HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini52 – 10453PeriplasmicSequence analysisAdd
BLAST
Transmembranei105 – 12420HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini125 – 1339CytoplasmicSequence analysis
Transmembranei134 – 15320HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini154 – 18431PeriplasmicSequence analysisAdd
BLAST
Transmembranei185 – 20420HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini205 – 23935CytoplasmicSequence analysisAdd
BLAST
Transmembranei240 – 25920HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini260 – 28425PeriplasmicSequence analysisAdd
BLAST
Transmembranei285 – 30420HelicalPROSITE-ProRule annotationAdd
BLAST
Topological domaini305 – 31713CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: UniProtKB-KW
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 317317Putrescine transport system permease protein PotHPRO_0000060188Add
BLAST

Proteomic databases

PaxDbiP31135.
PRIDEiP31135.

Interactioni

Protein-protein interaction databases

BioGridi4259995. 14 interactions.
IntActiP31135. 1 interaction.
STRINGi511145.b0856.

Structurei

3D structure databases

ProteinModelPortaliP31135.
SMRiP31135. Positions 81-297.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini99 – 305207ABC transmembrane type-1PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

Contains 1 ABC transmembrane type-1 domain.PROSITE-ProRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105F38. Bacteria.
COG1176. LUCA.
HOGENOMiHOG000263582.
InParanoidiP31135.
KOiK11075.
OMAiSNRDWPL.
OrthoDBiEOG6HMXBZ.
PhylomeDBiP31135.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31135-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSTLEPAAQS KPPGGFKLWL SQLQMKHGRK LVIALPYIWL ILLFLLPFLI
60 70 80 90 100
VFKISLAEMA RAIPPYTELM EWADGQLSIT LNLGNFLQLT DDPLYFDAYL
110 120 130 140 150
QSLQVAAIST FCCLLIGYPL AWAVAHSKPS TRNILLLLVI LPSWTSFLIR
160 170 180 190 200
VYAWMGILKN NGVLNNFLLW LGVIDQPLTI LHTNLAVYIG IVYAYVPFMV
210 220 230 240 250
LPIYTALIRI DYSLVEAALD LGARPLKTFF TVIVPLTKGG IIAGSMLVFI
260 270 280 290 300
PAVGEFVIPE LLGGPDSIMI GRVLWQEFFN NRDWPVASAV AIIMLLLLIV
310
PIMWFHKHQQ KSVGEHG
Length:317
Mass (Da):35,490
Last modified:July 1, 1993 - v1
Checksum:i5085C43C311F490A
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93239 Genomic DNA. Translation: AAA24411.1.
U00096 Genomic DNA. Translation: AAC73943.1.
AP009048 Genomic DNA. Translation: BAA35567.1.
PIRiC45313.
RefSeqiNP_415377.1. NC_000913.3.
WP_000105430.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73943; AAC73943; b0856.
BAA35567; BAA35567; BAA35567.
GeneIDi945475.
KEGGiecj:JW0840.
eco:b0856.
PATRICi32116917. VBIEscCol129921_0884.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93239 Genomic DNA. Translation: AAA24411.1.
U00096 Genomic DNA. Translation: AAC73943.1.
AP009048 Genomic DNA. Translation: BAA35567.1.
PIRiC45313.
RefSeqiNP_415377.1. NC_000913.3.
WP_000105430.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31135.
SMRiP31135. Positions 81-297.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259995. 14 interactions.
IntActiP31135. 1 interaction.
STRINGi511145.b0856.

Protein family/group databases

TCDBi3.A.1.11.2. the atp-binding cassette (abc) superfamily.

Proteomic databases

PaxDbiP31135.
PRIDEiP31135.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73943; AAC73943; b0856.
BAA35567; BAA35567; BAA35567.
GeneIDi945475.
KEGGiecj:JW0840.
eco:b0856.
PATRICi32116917. VBIEscCol129921_0884.

Organism-specific databases

EchoBASEiEB1588.
EcoGeneiEG11631. potH.

Phylogenomic databases

eggNOGiENOG4105F38. Bacteria.
COG1176. LUCA.
HOGENOMiHOG000263582.
InParanoidiP31135.
KOiK11075.
OMAiSNRDWPL.
OrthoDBiEOG6HMXBZ.
PhylomeDBiP31135.

Enzyme and pathway databases

BioCyciEcoCyc:POTH-MONOMER.
ECOL316407:JW0840-MONOMER.
MetaCyc:POTH-MONOMER.

Miscellaneous databases

PROiP31135.

Family and domain databases

Gene3Di1.10.3720.10. 1 hit.
InterProiIPR000515. MetI-like.
[Graphical view]
PfamiPF00528. BPD_transp_1. 1 hit.
[Graphical view]
SUPFAMiSSF161098. SSF161098. 1 hit.
PROSITEiPS50928. ABC_TM1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characteristics of the operon for a putrescine transport system that maps at 19 minutes on the Escherichia coli chromosome."
    Pistocchi R., Kashiwagi K., Miyamoto S., Nukui E., Sadakata Y., Kobayashi H., Igarashi K.
    J. Biol. Chem. 268:146-152(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiPOTH_ECOLI
AccessioniPrimary (citable) accession number: P31135
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 20, 2016
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.