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Protein

Putrescine-binding periplasmic protein

Gene

potF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei38Putrescine1 Publication1
Binding sitei247Putrescine1 Publication1
Binding sitei278Putrescine1 Publication1

GO - Molecular functioni

  • putrescine binding Source: EcoCyc

GO - Biological processi

  • putrescine transport Source: EcoCyc

Keywordsi

Biological processTransport

Enzyme and pathway databases

BioCyciEcoCyc:POTF-MONOMER
MetaCyc:POTF-MONOMER

Protein family/group databases

TCDBi3.A.1.11.2 the atp-binding cassette (abc) superfamily

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine-binding periplasmic protein
Gene namesi
Name:potF
Ordered Locus Names:b0854, JW0838
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11629 potF

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc

Keywords - Cellular componenti

Periplasm

Pathology & Biotechi

Chemistry databases

DrugBankiDB01917 Putrescine

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 262 PublicationsAdd BLAST26
ChainiPRO_000003184227 – 370Putrescine-binding periplasmic proteinAdd BLAST344

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi175 ↔ 2391 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP31133
PaxDbiP31133
PRIDEiP31133

2D gel databases

SWISS-2DPAGEP31133

Interactioni

Protein-protein interaction databases

BioGridi4259994, 13 interactors
DIPiDIP-10531N
IntActiP31133, 2 interactors
STRINGi316385.ECDH10B_0924

Structurei

Secondary structure

1370
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi31 – 37Combined sources7
Helixi45 – 53Combined sources9
Beta strandi56 – 63Combined sources8
Helixi65 – 74Combined sources10
Beta strandi80 – 82Combined sources3
Helixi86 – 92Combined sources7
Turni93 – 96Combined sources4
Helixi103 – 105Combined sources3
Helixi107 – 111Combined sources5
Helixi114 – 120Combined sources7
Turni121 – 123Combined sources3
Helixi125 – 127Combined sources3
Beta strandi129 – 143Combined sources15
Helixi144 – 151Combined sources8
Helixi161 – 164Combined sources4
Helixi166 – 172Combined sources7
Helixi173 – 175Combined sources3
Beta strandi177 – 179Combined sources3
Helixi183 – 193Combined sources11
Helixi203 – 207Combined sources5
Helixi209 – 217Combined sources9
Helixi218 – 220Combined sources3
Helixi228 – 234Combined sources7
Beta strandi239 – 244Combined sources6
Helixi245 – 258Combined sources14
Beta strandi264 – 267Combined sources4
Beta strandi273 – 281Combined sources9
Helixi289 – 300Combined sources12
Helixi302 – 312Combined sources11
Beta strandi315 – 318Combined sources4
Turni319 – 321Combined sources3
Helixi322 – 324Combined sources3
Helixi327 – 330Combined sources4
Turni333 – 335Combined sources3
Helixi339 – 342Combined sources4
Helixi353 – 368Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1A99X-ray2.20A/B/C/D27-370[»]
4JDFX-ray1.69A27-370[»]
ProteinModelPortaliP31133
SMRiP31133
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31133

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107RUE Bacteria
COG0687 LUCA
HOGENOMiHOG000263815
InParanoidiP31133
KOiK11073
OMAiNWAEYID
PhylomeDBiP31133

Family and domain databases

InterProiView protein in InterPro
IPR006059 SBP
IPR001188 Sperm_putr-bd
PfamiView protein in Pfam
PF13416 SBP_bac_8, 1 hit
PIRSFiPIRSF019574 Periplasmic_polyamine_BP, 1 hit
PRINTSiPR00909 SPERMDNBNDNG

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALNKKWLS GLVAGALMAV SVGTLAAEQK TLHIYNWSDY IAPDTVANFE
60 70 80 90 100
KETGIKVVYD VFDSNEVLEG KLMAGSTGFD LVVPSASFLE RQLTAGVFQP
110 120 130 140 150
LDKSKLPEWK NLDPELLKLV AKHDPDNKFA MPYMWATTGI GYNVDKVKAV
160 170 180 190 200
LGENAPVDSW DLILKPENLE KLKSCGVSFL DAPEEVFATV LNYLGKDPNS
210 220 230 240 250
TKADDYTGPA TDLLLKLRPN IRYFHSSQYI NDLANGDICV AIGWAGDVWQ
260 270 280 290 300
ASNRAKEAKN GVNVSFSIPK EGAMAFFDVF AMPADAKNKD EAYQFLNYLL
310 320 330 340 350
RPDVVAHISD HVFYANANKA ATPLVSAEVR ENPGIYPPAD VRAKLFTLKV
360 370
QDPKIDRVRT RAWTKVKSGK
Length:370
Mass (Da):40,840
Last modified:November 1, 1997 - v3
Checksum:iE7AA745A35BFDAD1
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti29Q → N AA sequence (PubMed:9298646).Curated1
Sequence conflicti216 – 217KL → NV in AAA24409 (PubMed:8416922).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93239 Genomic DNA Translation: AAA24409.1
U00096 Genomic DNA Translation: AAC73941.1
AP009048 Genomic DNA Translation: BAA35565.1
PIRiF64823
RefSeqiNP_415375.1, NC_000913.3
WP_000126072.1, NZ_LN832404.1

Genome annotation databases

EnsemblBacteriaiAAC73941; AAC73941; b0854
BAA35565; BAA35565; BAA35565
GeneIDi945480
KEGGiecj:JW0838
eco:b0854
PATRICifig|1411691.4.peg.1423

Entry informationi

Entry nameiPOTF_ECOLI
AccessioniPrimary (citable) accession number: P31133
Secondary accession number(s): P77289
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: March 28, 2018
This is version 142 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

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