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Protein

Putrescine-binding periplasmic protein

Gene

potF

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei38 – 381Putrescine1 Publication
Binding sitei247 – 2471Putrescine1 Publication
Binding sitei278 – 2781Putrescine1 Publication

GO - Molecular functioni

  • polyamine binding Source: InterPro
  • putrescine-importing ATPase activity Source: EcoCyc

GO - Biological processi

  • putrescine transport Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Transport

Enzyme and pathway databases

BioCyciEcoCyc:POTF-MONOMER.
ECOL316407:JW0838-MONOMER.
MetaCyc:POTF-MONOMER.
RETL1328306-WGS:GSTH-729-MONOMER.

Protein family/group databases

TCDBi3.A.1.11.2. the atp-binding cassette (abc) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Putrescine-binding periplasmic protein
Gene namesi
Name:potF
Ordered Locus Names:b0854, JW0838
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11629. potF.

Subcellular locationi

  • Periplasm 1 Publication

GO - Cellular componenti

  • outer membrane-bounded periplasmic space Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 26262 PublicationsAdd
BLAST
Chaini27 – 370344Putrescine-binding periplasmic proteinPRO_0000031842Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi175 ↔ 2391 Publication

Keywords - PTMi

Disulfide bond

Proteomic databases

EPDiP31133.
PaxDbiP31133.
PRIDEiP31133.

2D gel databases

SWISS-2DPAGEP31133.

Interactioni

Protein-protein interaction databases

BioGridi4259994. 13 interactions.
DIPiDIP-10531N.
IntActiP31133. 2 interactions.
STRINGi511145.b0854.

Structurei

Secondary structure

1
370
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi31 – 377Combined sources
Helixi45 – 539Combined sources
Beta strandi56 – 638Combined sources
Helixi65 – 7410Combined sources
Beta strandi80 – 823Combined sources
Helixi86 – 927Combined sources
Turni93 – 964Combined sources
Helixi103 – 1053Combined sources
Helixi107 – 1115Combined sources
Helixi114 – 1207Combined sources
Turni121 – 1233Combined sources
Helixi125 – 1273Combined sources
Beta strandi129 – 14315Combined sources
Helixi144 – 1518Combined sources
Helixi161 – 1644Combined sources
Helixi166 – 1727Combined sources
Helixi173 – 1753Combined sources
Beta strandi177 – 1793Combined sources
Helixi183 – 19311Combined sources
Helixi203 – 2075Combined sources
Helixi209 – 2179Combined sources
Helixi218 – 2203Combined sources
Helixi228 – 2347Combined sources
Beta strandi239 – 2446Combined sources
Helixi245 – 25814Combined sources
Beta strandi264 – 2674Combined sources
Beta strandi273 – 2819Combined sources
Helixi289 – 30012Combined sources
Helixi302 – 31211Combined sources
Beta strandi315 – 3184Combined sources
Turni319 – 3213Combined sources
Helixi322 – 3243Combined sources
Helixi327 – 3304Combined sources
Turni333 – 3353Combined sources
Helixi339 – 3424Combined sources
Helixi353 – 36816Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A99X-ray2.20A/B/C/D27-370[»]
4JDFX-ray1.69A27-370[»]
ProteinModelPortaliP31133.
SMRiP31133. Positions 29-369.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31133.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG4107RUE. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263815.
InParanoidiP31133.
KOiK11073.
OMAiVEYYIPK.
PhylomeDBiP31133.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31133-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTALNKKWLS GLVAGALMAV SVGTLAAEQK TLHIYNWSDY IAPDTVANFE
60 70 80 90 100
KETGIKVVYD VFDSNEVLEG KLMAGSTGFD LVVPSASFLE RQLTAGVFQP
110 120 130 140 150
LDKSKLPEWK NLDPELLKLV AKHDPDNKFA MPYMWATTGI GYNVDKVKAV
160 170 180 190 200
LGENAPVDSW DLILKPENLE KLKSCGVSFL DAPEEVFATV LNYLGKDPNS
210 220 230 240 250
TKADDYTGPA TDLLLKLRPN IRYFHSSQYI NDLANGDICV AIGWAGDVWQ
260 270 280 290 300
ASNRAKEAKN GVNVSFSIPK EGAMAFFDVF AMPADAKNKD EAYQFLNYLL
310 320 330 340 350
RPDVVAHISD HVFYANANKA ATPLVSAEVR ENPGIYPPAD VRAKLFTLKV
360 370
QDPKIDRVRT RAWTKVKSGK
Length:370
Mass (Da):40,840
Last modified:November 1, 1997 - v3
Checksum:iE7AA745A35BFDAD1
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti29 – 291Q → N AA sequence (PubMed:9298646).Curated
Sequence conflicti216 – 2172KL → NV in AAA24409 (PubMed:8416922).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93239 Genomic DNA. Translation: AAA24409.1.
U00096 Genomic DNA. Translation: AAC73941.1.
AP009048 Genomic DNA. Translation: BAA35565.1.
PIRiF64823.
RefSeqiNP_415375.1. NC_000913.3.
WP_000126072.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC73941; AAC73941; b0854.
BAA35565; BAA35565; BAA35565.
GeneIDi945480.
KEGGiecj:JW0838.
eco:b0854.
PATRICi32116913. VBIEscCol129921_0882.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M93239 Genomic DNA. Translation: AAA24409.1.
U00096 Genomic DNA. Translation: AAC73941.1.
AP009048 Genomic DNA. Translation: BAA35565.1.
PIRiF64823.
RefSeqiNP_415375.1. NC_000913.3.
WP_000126072.1. NZ_LN832404.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1A99X-ray2.20A/B/C/D27-370[»]
4JDFX-ray1.69A27-370[»]
ProteinModelPortaliP31133.
SMRiP31133. Positions 29-369.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259994. 13 interactions.
DIPiDIP-10531N.
IntActiP31133. 2 interactions.
STRINGi511145.b0854.

Protein family/group databases

TCDBi3.A.1.11.2. the atp-binding cassette (abc) superfamily.

2D gel databases

SWISS-2DPAGEP31133.

Proteomic databases

EPDiP31133.
PaxDbiP31133.
PRIDEiP31133.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC73941; AAC73941; b0854.
BAA35565; BAA35565; BAA35565.
GeneIDi945480.
KEGGiecj:JW0838.
eco:b0854.
PATRICi32116913. VBIEscCol129921_0882.

Organism-specific databases

EchoBASEiEB1586.
EcoGeneiEG11629. potF.

Phylogenomic databases

eggNOGiENOG4107RUE. Bacteria.
COG0687. LUCA.
HOGENOMiHOG000263815.
InParanoidiP31133.
KOiK11073.
OMAiVEYYIPK.
PhylomeDBiP31133.

Enzyme and pathway databases

BioCyciEcoCyc:POTF-MONOMER.
ECOL316407:JW0838-MONOMER.
MetaCyc:POTF-MONOMER.
RETL1328306-WGS:GSTH-729-MONOMER.

Miscellaneous databases

EvolutionaryTraceiP31133.
PROiP31133.

Family and domain databases

InterProiIPR001188. Sperm_putr-bd.
[Graphical view]
PIRSFiPIRSF019574. Periplasmic_polyamine_BP. 1 hit.
PRINTSiPR00909. SPERMDNBNDNG.
ProtoNetiSearch...

Entry informationi

Entry nameiPOTF_ECOLI
AccessioniPrimary (citable) accession number: P31133
Secondary accession number(s): P77289
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.