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P31125 (EAMA_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 107. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Probable amino-acid metabolite efflux pump
Gene names
Name:eamA
Synonyms:ydeD
Ordered Locus Names:b1533, JW5250
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length299 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

May be an export pump for cysteine and other metabolites of the cysteine pathway (such as N-acetyl-L-serine (NAS) and O-acetyl-L-serine (OAS)), and for other amino acids and their metabolites. Ref.5 Ref.6

Subcellular location

Cell inner membrane; Multi-pass membrane protein.

Sequence similarities

Belongs to the EamA transporter family.

Contains 2 EamA domains.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 299299Probable amino-acid metabolite efflux pump
PRO_0000108148

Regions

Topological domain1 – 66Cytoplasmic Potential
Transmembrane7 – 2721Helical; Potential
Topological domain28 – 314Periplasmic Potential
Transmembrane32 – 5221Helical; Potential
Topological domain53 – 597Cytoplasmic Potential
Transmembrane60 – 8021Helical; Potential
Topological domain81 – 877Periplasmic Potential
Transmembrane88 – 10821Helical; Potential
Topological domain109 – 1168Cytoplasmic Potential
Transmembrane117 – 13721Helical; Potential
Topological domain138 – 1403Periplasmic Potential
Transmembrane141 – 16121Helical; Potential
Topological domain162 – 17514Cytoplasmic Potential
Transmembrane176 – 19621Helical; Potential
Topological domain197 – 21620Periplasmic Potential
Transmembrane217 – 23721Helical; Potential
Topological domain238 – 2436Cytoplasmic Potential
Transmembrane244 – 26421Helical; Potential
Topological domain265 – 2684Periplasmic Potential
Transmembrane269 – 28921Helical; Potential
Topological domain290 – 29910Cytoplasmic Potential
Domain14 – 132119EamA 1
Domain153 – 287135EamA 2

Sequences

Sequence LengthMass (Da)Tools
P31125 [UniParc].

Last modified December 1, 2000. Version 2.
Checksum: 5FC73EFD2C08D565

FASTA29932,324
        10         20         30         40         50         60 
MSRKDGVLAL LVVVVWGLNF VVIKVGLHNM PPLMLAGLRF MLVAFPAIFF VARPKVPLNL 

        70         80         90        100        110        120 
LLGYGLTISF AQFAFLFCAI NFGMPAGLAS LVLQAQAFFT IMLGAFTFGE RLHGKQLAGI 

       130        140        150        160        170        180 
ALAIFGVLVL IEDSLNGQHV AMLGFMLTLA AAFSWACGNI FNKKIMSHST RPAVMSLVIW 

       190        200        210        220        230        240 
SALIPIIPFF VASLILDGSA TMIHSLVTID MTTILSLMYL AFVATIVGYG IWGTLLGRYE 

       250        260        270        280        290 
TWRVAPLSLL VPVVGLASAA LLLDERLTGL QFLGAVLIMT GLYINVFGLR WRKAVKVGS 

« Hide

References

« Hide 'large scale' references
[1]"Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
Cohen S.P., Haechler H., Levy S.B.
J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[3]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[4]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[5]"Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway."
Dassler T., Maier T., Winterhalter C., Boeck A.
Mol. Microbiol. 36:1101-1112(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: IDENTIFICATION OF START CODON, FUNCTION.
Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
[6]Dassler T.
Unpublished observations (JAN-2001)
Cited for: FUNCTION.
[7]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M96235 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74606.2.
AP009048 Genomic DNA. Translation: BAA15223.2.
PIRH64907.
RefSeqNP_416050.4. NC_000913.3.
YP_489796.1. NC_007779.1.

3D structure databases

ProteinModelPortalP31125.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b1533.

Protein family/group databases

TCDB2.A.7.3.2. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PRIDEP31125.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74606; AAC74606; b1533.
BAA15223; BAA15223; BAA15223.
GeneID12930118.
946081.
KEGGecj:Y75_p1508.
eco:b1533.
PATRIC32118364. VBIEscCol129921_1602.

Organism-specific databases

EchoBASEEB1594.
EcoGeneEG11639. eamA.

Phylogenomic databases

eggNOGCOG0697.
HOGENOMHOG000184107.
KOK15268.
OMAEGNDTTI.
OrthoDBEOG6QK4TK.
PhylomeDBP31125.

Enzyme and pathway databases

BioCycEcoCyc:EG11639-MONOMER.
ECOL316407:JW5250-MONOMER.
MetaCyc:EG11639-MONOMER.

Gene expression databases

GenevestigatorP31125.

Family and domain databases

InterProIPR000620. DMT.
[Graphical view]
PfamPF00892. EamA. 2 hits.
[Graphical view]
ProtoNetSearch...

Other

PROP31125.

Entry information

Entry nameEAMA_ECOLI
AccessionPrimary (citable) accession number: P31125
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 1, 2000
Last modified: July 9, 2014
This is version 107 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Uncharacterized protein families (UPF)

List of uncharacterized protein family (UPF) entries

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene