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P31125

- EAMA_ECOLI

UniProt

P31125 - EAMA_ECOLI

Protein

Probable amino-acid metabolite efflux pump

Gene

eamA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 108 (01 Oct 2014)
      Sequence version 2 (01 Dec 2000)
      Previous versions | rss
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    Functioni

    May be an export pump for cysteine and other metabolites of the cysteine pathway (such as N-acetyl-L-serine (NAS) and O-acetyl-L-serine (OAS)), and for other amino acids and their metabolites.2 Publications

    GO - Molecular functioni

    1. efflux transmembrane transporter activity Source: EcoCyc

    GO - Biological processi

    1. cysteine export Source: EcoCyc
    2. transmembrane transport Source: GOC

    Keywords - Biological processi

    Amino-acid transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:EG11639-MONOMER.
    ECOL316407:JW5250-MONOMER.
    MetaCyc:EG11639-MONOMER.

    Protein family/group databases

    TCDBi2.A.7.3.2. the drug/metabolite transporter (dmt) superfamily.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Probable amino-acid metabolite efflux pump
    Gene namesi
    Name:eamA
    Synonyms:ydeD
    Ordered Locus Names:b1533, JW5250
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11639. eamA.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: EcoCyc
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 299299Probable amino-acid metabolite efflux pumpPRO_0000108148Add
    BLAST

    Proteomic databases

    PRIDEiP31125.

    Expressioni

    Gene expression databases

    GenevestigatoriP31125.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b1533.

    Structurei

    3D structure databases

    ProteinModelPortaliP31125.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 66CytoplasmicSequence Analysis
    Topological domaini28 – 314PeriplasmicSequence Analysis
    Topological domaini53 – 597CytoplasmicSequence Analysis
    Topological domaini81 – 877PeriplasmicSequence Analysis
    Topological domaini109 – 1168CytoplasmicSequence Analysis
    Topological domaini138 – 1403PeriplasmicSequence Analysis
    Topological domaini162 – 17514CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini197 – 21620PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini238 – 2436CytoplasmicSequence Analysis
    Topological domaini265 – 2684PeriplasmicSequence Analysis
    Topological domaini290 – 29910CytoplasmicSequence Analysis

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei7 – 2721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei32 – 5221HelicalSequence AnalysisAdd
    BLAST
    Transmembranei60 – 8021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei88 – 10821HelicalSequence AnalysisAdd
    BLAST
    Transmembranei117 – 13721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei141 – 16121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei176 – 19621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei217 – 23721HelicalSequence AnalysisAdd
    BLAST
    Transmembranei244 – 26421HelicalSequence AnalysisAdd
    BLAST
    Transmembranei269 – 28921HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini14 – 132119EamA 1Add
    BLAST
    Domaini153 – 287135EamA 2Add
    BLAST

    Sequence similaritiesi

    Belongs to the EamA transporter family.Curated
    Contains 2 EamA domains.Curated

    Keywords - Domaini

    Repeat, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG0697.
    HOGENOMiHOG000184107.
    KOiK15268.
    OMAiEGNDTTI.
    OrthoDBiEOG6QK4TK.
    PhylomeDBiP31125.

    Family and domain databases

    InterProiIPR000620. DMT.
    [Graphical view]
    PfamiPF00892. EamA. 2 hits.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31125-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSRKDGVLAL LVVVVWGLNF VVIKVGLHNM PPLMLAGLRF MLVAFPAIFF    50
    VARPKVPLNL LLGYGLTISF AQFAFLFCAI NFGMPAGLAS LVLQAQAFFT 100
    IMLGAFTFGE RLHGKQLAGI ALAIFGVLVL IEDSLNGQHV AMLGFMLTLA 150
    AAFSWACGNI FNKKIMSHST RPAVMSLVIW SALIPIIPFF VASLILDGSA 200
    TMIHSLVTID MTTILSLMYL AFVATIVGYG IWGTLLGRYE TWRVAPLSLL 250
    VPVVGLASAA LLLDERLTGL QFLGAVLIMT GLYINVFGLR WRKAVKVGS 299
    Length:299
    Mass (Da):32,324
    Last modified:December 1, 2000 - v2
    Checksum:i5FC73EFD2C08D565
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96235 Genomic DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74606.2.
    AP009048 Genomic DNA. Translation: BAA15223.2.
    PIRiH64907.
    RefSeqiNP_416050.4. NC_000913.3.
    YP_489796.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74606; AAC74606; b1533.
    BAA15223; BAA15223; BAA15223.
    GeneIDi12930118.
    946081.
    KEGGiecj:Y75_p1508.
    eco:b1533.
    PATRICi32118364. VBIEscCol129921_1602.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M96235 Genomic DNA. No translation available.
    U00096 Genomic DNA. Translation: AAC74606.2 .
    AP009048 Genomic DNA. Translation: BAA15223.2 .
    PIRi H64907.
    RefSeqi NP_416050.4. NC_000913.3.
    YP_489796.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P31125.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b1533.

    Protein family/group databases

    TCDBi 2.A.7.3.2. the drug/metabolite transporter (dmt) superfamily.

    Proteomic databases

    PRIDEi P31125.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74606 ; AAC74606 ; b1533 .
    BAA15223 ; BAA15223 ; BAA15223 .
    GeneIDi 12930118.
    946081.
    KEGGi ecj:Y75_p1508.
    eco:b1533.
    PATRICi 32118364. VBIEscCol129921_1602.

    Organism-specific databases

    EchoBASEi EB1594.
    EcoGenei EG11639. eamA.

    Phylogenomic databases

    eggNOGi COG0697.
    HOGENOMi HOG000184107.
    KOi K15268.
    OMAi EGNDTTI.
    OrthoDBi EOG6QK4TK.
    PhylomeDBi P31125.

    Enzyme and pathway databases

    BioCyci EcoCyc:EG11639-MONOMER.
    ECOL316407:JW5250-MONOMER.
    MetaCyc:EG11639-MONOMER.

    Miscellaneous databases

    PROi P31125.

    Gene expression databases

    Genevestigatori P31125.

    Family and domain databases

    InterProi IPR000620. DMT.
    [Graphical view ]
    Pfami PF00892. EamA. 2 hits.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
      Cohen S.P., Haechler H., Levy S.B.
      J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    5. "Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway."
      Dassler T., Maier T., Winterhalter C., Boeck A.
      Mol. Microbiol. 36:1101-1112(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION OF START CODON, FUNCTION.
      Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
    6. Dassler T.
      Unpublished observations (JAN-2001)
      Cited for: FUNCTION.
    7. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiEAMA_ECOLI
    AccessioniPrimary (citable) accession number: P31125
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: December 1, 2000
    Last modified: October 1, 2014
    This is version 108 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families
    3. Uncharacterized protein families (UPF)
      List of uncharacterized protein family (UPF) entries

    External Data

    Dasty 3