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Protein

Probable amino-acid metabolite efflux pump

Gene

eamA

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

May be an export pump for cysteine and other metabolites of the cysteine pathway (such as N-acetyl-L-serine (NAS) and O-acetyl-L-serine (OAS)), and for other amino acids and their metabolites.2 Publications

GO - Molecular functioni

  • efflux transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  • cysteine export Source: EcoCyc
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11639-MONOMER.
ECOL316407:JW5250-MONOMER.
MetaCyc:EG11639-MONOMER.

Protein family/group databases

TCDBi2.A.7.3.2. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable amino-acid metabolite efflux pump
Gene namesi
Name:eamA
Synonyms:ydeD
Ordered Locus Names:b1533, JW5250
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11639. eamA.

Subcellular locationi

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 6CytoplasmicSequence analysis6
Transmembranei7 – 27HelicalSequence analysisAdd BLAST21
Topological domaini28 – 31PeriplasmicSequence analysis4
Transmembranei32 – 52HelicalSequence analysisAdd BLAST21
Topological domaini53 – 59CytoplasmicSequence analysis7
Transmembranei60 – 80HelicalSequence analysisAdd BLAST21
Topological domaini81 – 87PeriplasmicSequence analysis7
Transmembranei88 – 108HelicalSequence analysisAdd BLAST21
Topological domaini109 – 116CytoplasmicSequence analysis8
Transmembranei117 – 137HelicalSequence analysisAdd BLAST21
Topological domaini138 – 140PeriplasmicSequence analysis3
Transmembranei141 – 161HelicalSequence analysisAdd BLAST21
Topological domaini162 – 175CytoplasmicSequence analysisAdd BLAST14
Transmembranei176 – 196HelicalSequence analysisAdd BLAST21
Topological domaini197 – 216PeriplasmicSequence analysisAdd BLAST20
Transmembranei217 – 237HelicalSequence analysisAdd BLAST21
Topological domaini238 – 243CytoplasmicSequence analysis6
Transmembranei244 – 264HelicalSequence analysisAdd BLAST21
Topological domaini265 – 268PeriplasmicSequence analysis4
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Topological domaini290 – 299CytoplasmicSequence analysis10

GO - Cellular componenti

  • integral component of membrane Source: EcoCyc
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001081481 – 299Probable amino-acid metabolite efflux pumpAdd BLAST299

Proteomic databases

PaxDbiP31125.
PRIDEiP31125.

Interactioni

Protein-protein interaction databases

BioGridi4259112. 3 interactors.
STRINGi511145.b1533.

Structurei

3D structure databases

ProteinModelPortaliP31125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini14 – 132EamA 1Add BLAST119
Domaini153 – 287EamA 2Add BLAST135

Sequence similaritiesi

Belongs to the EamA transporter family.Curated
Contains 2 EamA domains.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4108594. Bacteria.
COG0697. LUCA.
HOGENOMiHOG000184107.
InParanoidiP31125.
KOiK15268.
OMAiMTISFGQ.
PhylomeDBiP31125.

Family and domain databases

InterProiIPR000620. EamA_dom.
[Graphical view]
PfamiPF00892. EamA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31125-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSRKDGVLAL LVVVVWGLNF VVIKVGLHNM PPLMLAGLRF MLVAFPAIFF
60 70 80 90 100
VARPKVPLNL LLGYGLTISF AQFAFLFCAI NFGMPAGLAS LVLQAQAFFT
110 120 130 140 150
IMLGAFTFGE RLHGKQLAGI ALAIFGVLVL IEDSLNGQHV AMLGFMLTLA
160 170 180 190 200
AAFSWACGNI FNKKIMSHST RPAVMSLVIW SALIPIIPFF VASLILDGSA
210 220 230 240 250
TMIHSLVTID MTTILSLMYL AFVATIVGYG IWGTLLGRYE TWRVAPLSLL
260 270 280 290
VPVVGLASAA LLLDERLTGL QFLGAVLIMT GLYINVFGLR WRKAVKVGS
Length:299
Mass (Da):32,324
Last modified:December 1, 2000 - v2
Checksum:i5FC73EFD2C08D565
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96235 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74606.2.
AP009048 Genomic DNA. Translation: BAA15223.2.
PIRiH64907.
RefSeqiNP_416050.4. NC_000913.3.
WP_000087187.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC74606; AAC74606; b1533.
BAA15223; BAA15223; BAA15223.
GeneIDi946081.
KEGGiecj:JW5250.
eco:b1533.
PATRICi32118364. VBIEscCol129921_1602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96235 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74606.2.
AP009048 Genomic DNA. Translation: BAA15223.2.
PIRiH64907.
RefSeqiNP_416050.4. NC_000913.3.
WP_000087187.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31125.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259112. 3 interactors.
STRINGi511145.b1533.

Protein family/group databases

TCDBi2.A.7.3.2. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PaxDbiP31125.
PRIDEiP31125.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74606; AAC74606; b1533.
BAA15223; BAA15223; BAA15223.
GeneIDi946081.
KEGGiecj:JW5250.
eco:b1533.
PATRICi32118364. VBIEscCol129921_1602.

Organism-specific databases

EchoBASEiEB1594.
EcoGeneiEG11639. eamA.

Phylogenomic databases

eggNOGiENOG4108594. Bacteria.
COG0697. LUCA.
HOGENOMiHOG000184107.
InParanoidiP31125.
KOiK15268.
OMAiMTISFGQ.
PhylomeDBiP31125.

Enzyme and pathway databases

BioCyciEcoCyc:EG11639-MONOMER.
ECOL316407:JW5250-MONOMER.
MetaCyc:EG11639-MONOMER.

Miscellaneous databases

PROiP31125.

Family and domain databases

InterProiIPR000620. EamA_dom.
[Graphical view]
PfamiPF00892. EamA. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiEAMA_ECOLI
AccessioniPrimary (citable) accession number: P31125
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 1, 2000
Last modified: November 2, 2016
This is version 120 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.