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P31125

- EAMA_ECOLI

UniProt

P31125 - EAMA_ECOLI

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Protein

Probable amino-acid metabolite efflux pump

Gene

eamA

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

May be an export pump for cysteine and other metabolites of the cysteine pathway (such as N-acetyl-L-serine (NAS) and O-acetyl-L-serine (OAS)), and for other amino acids and their metabolites.2 Publications

GO - Molecular functioni

  1. efflux transmembrane transporter activity Source: EcoCyc

GO - Biological processi

  1. cysteine export Source: EcoCyc
  2. transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Biological processi

Amino-acid transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:EG11639-MONOMER.
ECOL316407:JW5250-MONOMER.
MetaCyc:EG11639-MONOMER.

Protein family/group databases

TCDBi2.A.7.3.2. the drug/metabolite transporter (dmt) superfamily.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable amino-acid metabolite efflux pump
Gene namesi
Name:eamA
Synonyms:ydeD
Ordered Locus Names:b1533, JW5250
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

Organism-specific databases

EcoGeneiEG11639. eamA.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 66CytoplasmicSequence Analysis
Transmembranei7 – 2721HelicalSequence AnalysisAdd
BLAST
Topological domaini28 – 314PeriplasmicSequence Analysis
Transmembranei32 – 5221HelicalSequence AnalysisAdd
BLAST
Topological domaini53 – 597CytoplasmicSequence Analysis
Transmembranei60 – 8021HelicalSequence AnalysisAdd
BLAST
Topological domaini81 – 877PeriplasmicSequence Analysis
Transmembranei88 – 10821HelicalSequence AnalysisAdd
BLAST
Topological domaini109 – 1168CytoplasmicSequence Analysis
Transmembranei117 – 13721HelicalSequence AnalysisAdd
BLAST
Topological domaini138 – 1403PeriplasmicSequence Analysis
Transmembranei141 – 16121HelicalSequence AnalysisAdd
BLAST
Topological domaini162 – 17514CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei176 – 19621HelicalSequence AnalysisAdd
BLAST
Topological domaini197 – 21620PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei217 – 23721HelicalSequence AnalysisAdd
BLAST
Topological domaini238 – 2436CytoplasmicSequence Analysis
Transmembranei244 – 26421HelicalSequence AnalysisAdd
BLAST
Topological domaini265 – 2684PeriplasmicSequence Analysis
Transmembranei269 – 28921HelicalSequence AnalysisAdd
BLAST
Topological domaini290 – 29910CytoplasmicSequence Analysis

GO - Cellular componenti

  1. integral component of membrane Source: EcoCyc
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 299299Probable amino-acid metabolite efflux pumpPRO_0000108148Add
BLAST

Proteomic databases

PRIDEiP31125.

Expressioni

Gene expression databases

GenevestigatoriP31125.

Interactioni

Protein-protein interaction databases

STRINGi511145.b1533.

Structurei

3D structure databases

ProteinModelPortaliP31125.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini14 – 132119EamA 1Add
BLAST
Domaini153 – 287135EamA 2Add
BLAST

Sequence similaritiesi

Belongs to the EamA transporter family.Curated
Contains 2 EamA domains.Curated

Keywords - Domaini

Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0697.
HOGENOMiHOG000184107.
InParanoidiP31125.
KOiK15268.
OMAiEGNDTTI.
OrthoDBiEOG6QK4TK.
PhylomeDBiP31125.

Family and domain databases

InterProiIPR000620. DMT.
[Graphical view]
PfamiPF00892. EamA. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31125-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MSRKDGVLAL LVVVVWGLNF VVIKVGLHNM PPLMLAGLRF MLVAFPAIFF
60 70 80 90 100
VARPKVPLNL LLGYGLTISF AQFAFLFCAI NFGMPAGLAS LVLQAQAFFT
110 120 130 140 150
IMLGAFTFGE RLHGKQLAGI ALAIFGVLVL IEDSLNGQHV AMLGFMLTLA
160 170 180 190 200
AAFSWACGNI FNKKIMSHST RPAVMSLVIW SALIPIIPFF VASLILDGSA
210 220 230 240 250
TMIHSLVTID MTTILSLMYL AFVATIVGYG IWGTLLGRYE TWRVAPLSLL
260 270 280 290
VPVVGLASAA LLLDERLTGL QFLGAVLIMT GLYINVFGLR WRKAVKVGS
Length:299
Mass (Da):32,324
Last modified:December 1, 2000 - v2
Checksum:i5FC73EFD2C08D565
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96235 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74606.2.
AP009048 Genomic DNA. Translation: BAA15223.2.
PIRiH64907.
RefSeqiNP_416050.4. NC_000913.3.
YP_489796.1. NC_007779.1.

Genome annotation databases

EnsemblBacteriaiAAC74606; AAC74606; b1533.
BAA15223; BAA15223; BAA15223.
GeneIDi12930118.
946081.
KEGGiecj:Y75_p1508.
eco:b1533.
PATRICi32118364. VBIEscCol129921_1602.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M96235 Genomic DNA. No translation available.
U00096 Genomic DNA. Translation: AAC74606.2 .
AP009048 Genomic DNA. Translation: BAA15223.2 .
PIRi H64907.
RefSeqi NP_416050.4. NC_000913.3.
YP_489796.1. NC_007779.1.

3D structure databases

ProteinModelPortali P31125.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 511145.b1533.

Protein family/group databases

TCDBi 2.A.7.3.2. the drug/metabolite transporter (dmt) superfamily.

Proteomic databases

PRIDEi P31125.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai AAC74606 ; AAC74606 ; b1533 .
BAA15223 ; BAA15223 ; BAA15223 .
GeneIDi 12930118.
946081.
KEGGi ecj:Y75_p1508.
eco:b1533.
PATRICi 32118364. VBIEscCol129921_1602.

Organism-specific databases

EchoBASEi EB1594.
EcoGenei EG11639. eamA.

Phylogenomic databases

eggNOGi COG0697.
HOGENOMi HOG000184107.
InParanoidi P31125.
KOi K15268.
OMAi EGNDTTI.
OrthoDBi EOG6QK4TK.
PhylomeDBi P31125.

Enzyme and pathway databases

BioCyci EcoCyc:EG11639-MONOMER.
ECOL316407:JW5250-MONOMER.
MetaCyc:EG11639-MONOMER.

Miscellaneous databases

PROi P31125.

Gene expression databases

Genevestigatori P31125.

Family and domain databases

InterProi IPR000620. DMT.
[Graphical view ]
Pfami PF00892. EamA. 2 hits.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
    Cohen S.P., Haechler H., Levy S.B.
    J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  4. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  5. "Identification of a major facilitator protein from Escherichia coli involved in efflux of metabolites of the cysteine pathway."
    Dassler T., Maier T., Winterhalter C., Boeck A.
    Mol. Microbiol. 36:1101-1112(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION OF START CODON, FUNCTION.
    Strain: K12 / MC4100 / ATCC 35695 / DSM 6574.
  6. Dassler T.
    Unpublished observations (JAN-2001)
    Cited for: FUNCTION.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiEAMA_ECOLI
AccessioniPrimary (citable) accession number: P31125
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 1, 2000
Last modified: October 29, 2014
This is version 109 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families
  3. Uncharacterized protein families (UPF)
    List of uncharacterized protein family (UPF) entries

External Data

Dasty 3