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P31122

- SOTB_ECOLI

UniProt

P31122 - SOTB_ECOLI

Protein

Sugar efflux transporter

Gene

sotB

Organism
Escherichia coli (strain K12)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    Involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites. Transports L-arabinose and to a lesser extent IPTG. Seems to contribute to the control of the arabinose regulon.

    GO - Molecular functioni

    1. carbohydrate transmembrane transporter activity Source: UniProtKB-HAMAP

    Keywords - Biological processi

    Sugar transport, Transport

    Enzyme and pathway databases

    BioCyciEcoCyc:YDEA-MONOMER.
    ECOL316407:JW1521-MONOMER.
    MetaCyc:YDEA-MONOMER.

    Protein family/group databases

    TCDBi2.A.1.2.15. the major facilitator superfamily (mfs).

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Sugar efflux transporter
    Gene namesi
    Name:sotB
    Synonyms:ydeA
    Ordered Locus Names:b1528, JW1521
    OrganismiEscherichia coli (strain K12)
    Taxonomic identifieri83333 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
    ProteomesiUP000000318: Chromosome, UP000000625: Chromosome

    Organism-specific databases

    EcoGeneiEG11636. sotB.

    Subcellular locationi

    GO - Cellular componenti

    1. integral component of membrane Source: UniProtKB-KW
    2. plasma membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cell inner membrane, Cell membrane, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 396396Sugar efflux transporterPRO_0000209323Add
    BLAST

    Expressioni

    Gene expression databases

    GenevestigatoriP31122.

    Interactioni

    Protein-protein interaction databases

    STRINGi511145.b1528.

    Structurei

    3D structure databases

    ProteinModelPortaliP31122.
    ModBaseiSearch...
    MobiDBiSearch...

    Topological domain

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini36 – 4914PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini71 – 8010CytoplasmicSequence Analysis
    Topological domaini102 – 1021PeriplasmicSequence Analysis
    Topological domaini124 – 13512CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini157 – 16913PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini191 – 20818CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini230 – 24516PeriplasmicSequence AnalysisAdd
    BLAST
    Topological domaini267 – 2748CytoplasmicSequence Analysis
    Topological domaini296 – 2983PeriplasmicSequence Analysis
    Topological domaini320 – 33213CytoplasmicSequence AnalysisAdd
    BLAST
    Topological domaini354 – 36310PeriplasmicSequence Analysis
    Topological domaini385 – 39612CytoplasmicSequence AnalysisAdd
    BLAST

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei15 – 3521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei50 – 7021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei81 – 10121HelicalSequence AnalysisAdd
    BLAST
    Transmembranei103 – 12321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei136 – 15621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei170 – 19021HelicalSequence AnalysisAdd
    BLAST
    Transmembranei209 – 22921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei246 – 26621HelicalSequence AnalysisAdd
    BLAST
    Transmembranei275 – 29521HelicalSequence AnalysisAdd
    BLAST
    Transmembranei299 – 31921HelicalSequence AnalysisAdd
    BLAST
    Transmembranei333 – 35321HelicalSequence AnalysisAdd
    BLAST
    Transmembranei364 – 38421HelicalSequence AnalysisAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG2814.
    HOGENOMiHOG000268498.
    KOiK08159.
    OMAiGNDIRAN.
    OrthoDBiEOG6TJ7VJ.
    PhylomeDBiP31122.

    Family and domain databases

    HAMAPiMF_00517. MFS_SotB.
    InterProiIPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR023495. Sugar_effux_transptr_put.
    [Graphical view]
    PfamiPF07690. MFS_1. 1 hit.
    [Graphical view]
    SUPFAMiSSF103473. SSF103473. 1 hit.
    PROSITEiPS50850. MFS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31122-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTNTVSRKV AWLRVVTLAV AAFIFNTTEF VPVGLLSDIA QSFHMQTAQV    50
    GIMLTIYAWV VALMSLPFML MTSQVERRKL LICLFVVFIA SHVLSFLSWS 100
    FTVLVISRIG VAFAHAIFWS ITASLAIRMA PAGKRAQALS LIATGTALAM 150
    VLGLPLGRIV GQYFGWRMTF FAIGIGALIT LLCLIKLLPL LPSEHSGSLK 200
    SLPLLFRRPA LMSIYLLTVV VVTAHYTAYS YIEPFVQNIA GFSANFATAL 250
    LLLLGGAGII GSVIFGKLGN QYASALVSTA IALLLVCLAL LLPAANSEIH 300
    LGVLSIFWGI AMMIIGLGMQ VKVLALAPDA TDVAMALFSG IFNIGIGAGA 350
    LVGNQVSLHW SMSMIGYVGA VPAFAALIWS IIIFRRWPVT LEEQTQ 396
    Length:396
    Mass (Da):42,538
    Last modified:November 1, 1997 - v2
    Checksum:iCB6A34CA4EE6D4F0
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74601.1.
    AP009048 Genomic DNA. Translation: BAA15210.1.
    M96235 Genomic DNA. No translation available.
    PIRiC64907.
    RefSeqiNP_416045.1. NC_000913.3.
    YP_489791.1. NC_007779.1.

    Genome annotation databases

    EnsemblBacteriaiAAC74601; AAC74601; b1528.
    BAA15210; BAA15210; BAA15210.
    GeneIDi12930477.
    947218.
    KEGGiecj:Y75_p1503.
    eco:b1528.
    PATRICi32118354. VBIEscCol129921_1597.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U00096 Genomic DNA. Translation: AAC74601.1 .
    AP009048 Genomic DNA. Translation: BAA15210.1 .
    M96235 Genomic DNA. No translation available.
    PIRi C64907.
    RefSeqi NP_416045.1. NC_000913.3.
    YP_489791.1. NC_007779.1.

    3D structure databases

    ProteinModelPortali P31122.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 511145.b1528.

    Protein family/group databases

    TCDBi 2.A.1.2.15. the major facilitator superfamily (mfs).

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai AAC74601 ; AAC74601 ; b1528 .
    BAA15210 ; BAA15210 ; BAA15210 .
    GeneIDi 12930477.
    947218.
    KEGGi ecj:Y75_p1503.
    eco:b1528.
    PATRICi 32118354. VBIEscCol129921_1597.

    Organism-specific databases

    EchoBASEi EB1592.
    EcoGenei EG11636. sotB.

    Phylogenomic databases

    eggNOGi COG2814.
    HOGENOMi HOG000268498.
    KOi K08159.
    OMAi GNDIRAN.
    OrthoDBi EOG6TJ7VJ.
    PhylomeDBi P31122.

    Enzyme and pathway databases

    BioCyci EcoCyc:YDEA-MONOMER.
    ECOL316407:JW1521-MONOMER.
    MetaCyc:YDEA-MONOMER.

    Miscellaneous databases

    PROi P31122.

    Gene expression databases

    Genevestigatori P31122.

    Family and domain databases

    HAMAPi MF_00517. MFS_SotB.
    InterProi IPR011701. MFS.
    IPR020846. MFS_dom.
    IPR016196. MFS_dom_general_subst_transpt.
    IPR023495. Sugar_effux_transptr_put.
    [Graphical view ]
    Pfami PF07690. MFS_1. 1 hit.
    [Graphical view ]
    SUPFAMi SSF103473. SSF103473. 1 hit.
    PROSITEi PS50850. MFS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / MG1655 / ATCC 47076.
    3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
      Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
      Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
    4. "Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
      Cohen S.P., Haechler H., Levy S.B.
      J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-396.
    5. "Transcriptional activation of ydeA, which encodes a member of the major facilitator superfamily, interferes with arabinose accumulation and induction of the Escherichia coli arabinose PBAD promoter."
      Bost S., Silva F., Belin D.
      J. Bacteriol. 181:2185-2191(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: SB0.
    6. "Escherichia coli gene ydeA encodes a major facilitator pump which exports L-arabinose and isopropyl-beta-D-thiogalactopyranoside."
      Carole S., Pichoff S., Bouche J.-P.
      J. Bacteriol. 181:5123-5125(1999) [PubMed] [Europe PMC] [Abstract]
      Cited for: CHARACTERIZATION.
      Strain: JS219.
    7. "Global topology analysis of the Escherichia coli inner membrane proteome."
      Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
      Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
      Strain: K12 / MG1655 / ATCC 47076.

    Entry informationi

    Entry nameiSOTB_ECOLI
    AccessioniPrimary (citable) accession number: P31122
    Secondary accession number(s): P76883, P77353
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Escherichia coli
      Escherichia coli (strain K12): entries and cross-references to EcoGene
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3