Sugar efflux transporter - Escherichia coli (strain K12)

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P31122 (SOTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 105. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Sugar efflux transporter

Gene names

Name:	sotB
Synonyms:	ydeA
Ordered Locus Names:	b1528, JW1521

Organism

Escherichia coli (strain K12) [Reference proteome] [HAMAP]

Taxonomic identifier

83333 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Escherichia ›

Protein attributes

Sequence length	396 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites. Transports L-arabinose and to a lesser extent IPTG. Seems to contribute to the control of the arabinose regulon. HAMAP-Rule MF_00517
Subcellular location	Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_00517.
Sequence similarities	Belongs to the major facilitator superfamily. SotB (TC 2.A.1.2) family. [View classification]

Ontologies

Keywords
Biological process	Sugar transport Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Domain	Transmembrane Transmembrane helix
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate transmembrane transport Inferred from electronic annotation. Source: GOC
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	carbohydrate transmembrane transporter activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 396

396

Sugar efflux transporter HAMAP-Rule MF_00517

PRO_0000209323

Regions

Topological domain

1 – 14

Cytoplasmic Potential

Transmembrane

15 – 35

Helical; Potential

Topological domain

36 – 49

Periplasmic Potential

Transmembrane

50 – 70

Helical; Potential

Topological domain

71 – 80

Cytoplasmic Potential

Transmembrane

81 – 101

Helical; Potential

Topological domain

102

Periplasmic Potential

Transmembrane

103 – 123

Helical; Potential

Topological domain

124 – 135

Cytoplasmic Potential

Transmembrane

136 – 156

Helical; Potential

Topological domain

157 – 169

Periplasmic Potential

Transmembrane

170 – 190

Helical; Potential

Topological domain

191 – 208

Cytoplasmic Potential

Transmembrane

209 – 229

Helical; Potential

Topological domain

230 – 245

Periplasmic Potential

Transmembrane

246 – 266

Helical; Potential

Topological domain

267 – 274

Cytoplasmic Potential

Transmembrane

275 – 295

Helical; Potential

Topological domain

296 – 298

Periplasmic Potential

Transmembrane

299 – 319

Helical; Potential

Topological domain

320 – 332

Cytoplasmic Potential

Transmembrane

333 – 353

Helical; Potential

Topological domain

354 – 363

Periplasmic Potential

Transmembrane

364 – 384

Helical; Potential

Topological domain

385 – 396

Cytoplasmic Potential

Sequences

Sequence

Length

Mass (Da)

Tools

P31122 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: CB6A34CA4EE6D4F0
Show »

FASTA

396

42,538

        10         20         30         40         50         60 
MTTNTVSRKV AWLRVVTLAV AAFIFNTTEF VPVGLLSDIA QSFHMQTAQV GIMLTIYAWV 

        70         80         90        100        110        120 
VALMSLPFML MTSQVERRKL LICLFVVFIA SHVLSFLSWS FTVLVISRIG VAFAHAIFWS 

       130        140        150        160        170        180 
ITASLAIRMA PAGKRAQALS LIATGTALAM VLGLPLGRIV GQYFGWRMTF FAIGIGALIT 

       190        200        210        220        230        240 
LLCLIKLLPL LPSEHSGSLK SLPLLFRRPA LMSIYLLTVV VVTAHYTAYS YIEPFVQNIA 

       250        260        270        280        290        300 
GFSANFATAL LLLLGGAGII GSVIFGKLGN QYASALVSTA IALLLVCLAL LLPAANSEIH 

       310        320        330        340        350        360 
LGVLSIFWGI AMMIIGLGMQ VKVLALAPDA TDVAMALFSG IFNIGIGAGA LVGNQVSLHW 

       370        380        390 
SMSMIGYVGA VPAFAALIWS IIIFRRWPVT LEEQTQ

« Hide

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map." Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. Horiuchi T. DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]	"The complete genome sequence of Escherichia coli K-12." Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y. Science 277:1453-1474(1997) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / MG1655 / ATCC 47076.
[3]	"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110." Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T. Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]	"Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli." Cohen S.P., Haechler H., Levy S.B. J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-396.
[5]	"Transcriptional activation of ydeA, which encodes a member of the major facilitator superfamily, interferes with arabinose accumulation and induction of the Escherichia coli arabinose PBAD promoter." Bost S., Silva F., Belin D. J. Bacteriol. 181:2185-2191(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: SB0.
[6]	"Escherichia coli gene ydeA encodes a major facilitator pump which exports L-arabinose and isopropyl-beta-D-thiogalactopyranoside." Carole S., Pichoff S., Bouche J.-P. J. Bacteriol. 181:5123-5125(1999) [PubMed] [Europe PMC] [Abstract] Cited for: CHARACTERIZATION. Strain: JS219.
[7]	"Global topology analysis of the Escherichia coli inner membrane proteome." Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G. Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract] Cited for: TOPOLOGY [LARGE SCALE ANALYSIS]. Strain: K12 / MG1655 / ATCC 47076.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U00096 Genomic DNA. Translation: AAC74601.1. AP009048 Genomic DNA. Translation: BAA15210.1. M96235 Genomic DNA. No translation available.
PIR	C64907.
RefSeq	NP_416045.1. NC_000913.2. YP_489791.1. NC_007779.1.
3D structure databases
ProteinModelPortal	P31122.
ModBase	Search...
Protein-protein interaction databases
STRING	511145.b1528.
Protein family/group databases
TCDB	2.A.1.2.15. major facilitator superfamily (MFS).
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAC74601; AAC74601; b1528. BAA15210; BAA15210; BAA15210.
GeneID	12930477. 947218.
KEGG	ecj:Y75_p1503. eco:b1528.
PATRIC	32118354. VBIEscCol129921_1597.
Organism-specific databases
EchoBASE	EB1592.
EcoGene	EG11636. sotB.
Phylogenomic databases
eggNOG	COG2814.
HOGENOM	HOG000268498.
KO	K08159.
OMA	EALGWRT.
ProtClustDB	PRK03545.
Enzyme and pathway databases
BioCyc	EcoCyc:YDEA-MONOMER. ECOL316407:JW1521-MONOMER.
Gene expression databases
Genevestigator	P31122.
Family and domain databases
HAMAP	MF_00517. MFS_SotB.
InterPro	IPR011701. MFS. IPR020846. MFS_dom. IPR016196. MFS_dom_general_subst_transpt. IPR023495. Sugar_effux_transptr_put. [Graphical view]
Pfam	PF07690. MFS_1. 1 hit. [Graphical view]
SUPFAM	SSF103473. MFS_gen_substrate_transporter. 1 hit.
PROSITE	PS50850. MFS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SOTB_ECOLI

Accession

Primary (citable) accession number: P31122
Secondary accession number(s): P76883, P77353

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	November 1, 1997
Last modified:	May 1, 2013
This is version 105 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program

Relevant documents

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents