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Protein

Sugar efflux transporter

Gene

sotB

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites. Transports L-arabinose and to a lesser extent IPTG. Seems to contribute to the control of the arabinose regulon.

GO - Molecular functioni

Complete GO annotation...

Keywords - Biological processi

Sugar transport, Transport

Enzyme and pathway databases

BioCyciEcoCyc:YDEA-MONOMER.
ECOL316407:JW1521-MONOMER.
MetaCyc:YDEA-MONOMER.

Protein family/group databases

TCDBi2.A.1.2.15. the major facilitator superfamily (mfs).

Names & Taxonomyi

Protein namesi
Recommended name:
Sugar efflux transporter
Gene namesi
Name:sotB
Synonyms:ydeA
Ordered Locus Names:b1528, JW1521
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
ProteomesiUP000000318 Componenti: Chromosome UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11636. sotB.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 1414CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei15 – 3521HelicalSequence AnalysisAdd
BLAST
Topological domaini36 – 4914PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei50 – 7021HelicalSequence AnalysisAdd
BLAST
Topological domaini71 – 8010CytoplasmicSequence Analysis
Transmembranei81 – 10121HelicalSequence AnalysisAdd
BLAST
Topological domaini102 – 1021PeriplasmicSequence Analysis
Transmembranei103 – 12321HelicalSequence AnalysisAdd
BLAST
Topological domaini124 – 13512CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei136 – 15621HelicalSequence AnalysisAdd
BLAST
Topological domaini157 – 16913PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei170 – 19021HelicalSequence AnalysisAdd
BLAST
Topological domaini191 – 20818CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei209 – 22921HelicalSequence AnalysisAdd
BLAST
Topological domaini230 – 24516PeriplasmicSequence AnalysisAdd
BLAST
Transmembranei246 – 26621HelicalSequence AnalysisAdd
BLAST
Topological domaini267 – 2748CytoplasmicSequence Analysis
Transmembranei275 – 29521HelicalSequence AnalysisAdd
BLAST
Topological domaini296 – 2983PeriplasmicSequence Analysis
Transmembranei299 – 31921HelicalSequence AnalysisAdd
BLAST
Topological domaini320 – 33213CytoplasmicSequence AnalysisAdd
BLAST
Transmembranei333 – 35321HelicalSequence AnalysisAdd
BLAST
Topological domaini354 – 36310PeriplasmicSequence Analysis
Transmembranei364 – 38421HelicalSequence AnalysisAdd
BLAST
Topological domaini385 – 39612CytoplasmicSequence AnalysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 396396Sugar efflux transporterPRO_0000209323Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi511145.b1528.

Structurei

3D structure databases

ProteinModelPortaliP31122.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG2814.
HOGENOMiHOG000268498.
InParanoidiP31122.
KOiK08159.
OMAiYSSKYPA.
OrthoDBiEOG6TJ7VJ.
PhylomeDBiP31122.

Family and domain databases

HAMAPiMF_00517. MFS_SotB.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR023495. Sugar_effux_transptr_put.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31122-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTNTVSRKV AWLRVVTLAV AAFIFNTTEF VPVGLLSDIA QSFHMQTAQV
60 70 80 90 100
GIMLTIYAWV VALMSLPFML MTSQVERRKL LICLFVVFIA SHVLSFLSWS
110 120 130 140 150
FTVLVISRIG VAFAHAIFWS ITASLAIRMA PAGKRAQALS LIATGTALAM
160 170 180 190 200
VLGLPLGRIV GQYFGWRMTF FAIGIGALIT LLCLIKLLPL LPSEHSGSLK
210 220 230 240 250
SLPLLFRRPA LMSIYLLTVV VVTAHYTAYS YIEPFVQNIA GFSANFATAL
260 270 280 290 300
LLLLGGAGII GSVIFGKLGN QYASALVSTA IALLLVCLAL LLPAANSEIH
310 320 330 340 350
LGVLSIFWGI AMMIIGLGMQ VKVLALAPDA TDVAMALFSG IFNIGIGAGA
360 370 380 390
LVGNQVSLHW SMSMIGYVGA VPAFAALIWS IIIFRRWPVT LEEQTQ
Length:396
Mass (Da):42,538
Last modified:November 1, 1997 - v2
Checksum:iCB6A34CA4EE6D4F0
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74601.1.
AP009048 Genomic DNA. Translation: BAA15210.1.
M96235 Genomic DNA. No translation available.
PIRiC64907.
RefSeqiNP_416045.1. NC_000913.3.

Genome annotation databases

EnsemblBacteriaiAAC74601; AAC74601; b1528.
BAA15210; BAA15210; BAA15210.
GeneIDi947218.
KEGGiecj:Y75_p1503.
eco:b1528.
PATRICi32118354. VBIEscCol129921_1597.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U00096 Genomic DNA. Translation: AAC74601.1.
AP009048 Genomic DNA. Translation: BAA15210.1.
M96235 Genomic DNA. No translation available.
PIRiC64907.
RefSeqiNP_416045.1. NC_000913.3.

3D structure databases

ProteinModelPortaliP31122.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi511145.b1528.

Protein family/group databases

TCDBi2.A.1.2.15. the major facilitator superfamily (mfs).

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC74601; AAC74601; b1528.
BAA15210; BAA15210; BAA15210.
GeneIDi947218.
KEGGiecj:Y75_p1503.
eco:b1528.
PATRICi32118354. VBIEscCol129921_1597.

Organism-specific databases

EchoBASEiEB1592.
EcoGeneiEG11636. sotB.

Phylogenomic databases

eggNOGiCOG2814.
HOGENOMiHOG000268498.
InParanoidiP31122.
KOiK08159.
OMAiYSSKYPA.
OrthoDBiEOG6TJ7VJ.
PhylomeDBiP31122.

Enzyme and pathway databases

BioCyciEcoCyc:YDEA-MONOMER.
ECOL316407:JW1521-MONOMER.
MetaCyc:YDEA-MONOMER.

Miscellaneous databases

PROiP31122.

Family and domain databases

HAMAPiMF_00517. MFS_SotB.
InterProiIPR011701. MFS.
IPR020846. MFS_dom.
IPR023495. Sugar_effux_transptr_put.
[Graphical view]
PfamiPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMiSSF103473. SSF103473. 1 hit.
PROSITEiPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / MG1655 / ATCC 47076.
  3. "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
    Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
    Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
  4. "Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
    Cohen S.P., Haechler H., Levy S.B.
    J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-396.
  5. "Transcriptional activation of ydeA, which encodes a member of the major facilitator superfamily, interferes with arabinose accumulation and induction of the Escherichia coli arabinose PBAD promoter."
    Bost S., Silva F., Belin D.
    J. Bacteriol. 181:2185-2191(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: SB0.
  6. "Escherichia coli gene ydeA encodes a major facilitator pump which exports L-arabinose and isopropyl-beta-D-thiogalactopyranoside."
    Carole S., Pichoff S., Bouche J.-P.
    J. Bacteriol. 181:5123-5125(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION.
    Strain: JS219.
  7. "Global topology analysis of the Escherichia coli inner membrane proteome."
    Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
    Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
    Strain: K12 / MG1655 / ATCC 47076.

Entry informationi

Entry nameiSOTB_ECOLI
AccessioniPrimary (citable) accession number: P31122
Secondary accession number(s): P76883, P77353
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: June 24, 2015
This is version 121 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.