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P31122 (SOTB_ECOLI) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 116. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Sugar efflux transporter
Gene names
Name:sotB
Synonyms:ydeA
Ordered Locus Names:b1528, JW1521
OrganismEscherichia coli (strain K12) [Reference proteome] [HAMAP]
Taxonomic identifier83333 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia

Protein attributes

Sequence length396 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Involved in the efflux of sugars. The physiological role may be the reduction of the intracellular concentration of toxic sugars or sugar metabolites. Transports L-arabinose and to a lesser extent IPTG. Seems to contribute to the control of the arabinose regulon. HAMAP-Rule MF_00517

Subcellular location

Cell inner membrane; Multi-pass membrane protein HAMAP-Rule MF_00517.

Sequence similarities

Belongs to the major facilitator superfamily. SotB (TC 2.A.1.2) family. [View classification]

Ontologies

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 396396Sugar efflux transporter HAMAP-Rule MF_00517
PRO_0000209323

Regions

Topological domain1 – 1414Cytoplasmic Potential
Transmembrane15 – 3521Helical; Potential
Topological domain36 – 4914Periplasmic Potential
Transmembrane50 – 7021Helical; Potential
Topological domain71 – 8010Cytoplasmic Potential
Transmembrane81 – 10121Helical; Potential
Topological domain1021Periplasmic Potential
Transmembrane103 – 12321Helical; Potential
Topological domain124 – 13512Cytoplasmic Potential
Transmembrane136 – 15621Helical; Potential
Topological domain157 – 16913Periplasmic Potential
Transmembrane170 – 19021Helical; Potential
Topological domain191 – 20818Cytoplasmic Potential
Transmembrane209 – 22921Helical; Potential
Topological domain230 – 24516Periplasmic Potential
Transmembrane246 – 26621Helical; Potential
Topological domain267 – 2748Cytoplasmic Potential
Transmembrane275 – 29521Helical; Potential
Topological domain296 – 2983Periplasmic Potential
Transmembrane299 – 31921Helical; Potential
Topological domain320 – 33213Cytoplasmic Potential
Transmembrane333 – 35321Helical; Potential
Topological domain354 – 36310Periplasmic Potential
Transmembrane364 – 38421Helical; Potential
Topological domain385 – 39612Cytoplasmic Potential

Sequences

Sequence LengthMass (Da)Tools
P31122 [UniParc].

Last modified November 1, 1997. Version 2.
Checksum: CB6A34CA4EE6D4F0

FASTA39642,538
        10         20         30         40         50         60 
MTTNTVSRKV AWLRVVTLAV AAFIFNTTEF VPVGLLSDIA QSFHMQTAQV GIMLTIYAWV 

        70         80         90        100        110        120 
VALMSLPFML MTSQVERRKL LICLFVVFIA SHVLSFLSWS FTVLVISRIG VAFAHAIFWS 

       130        140        150        160        170        180 
ITASLAIRMA PAGKRAQALS LIATGTALAM VLGLPLGRIV GQYFGWRMTF FAIGIGALIT 

       190        200        210        220        230        240 
LLCLIKLLPL LPSEHSGSLK SLPLLFRRPA LMSIYLLTVV VVTAHYTAYS YIEPFVQNIA 

       250        260        270        280        290        300 
GFSANFATAL LLLLGGAGII GSVIFGKLGN QYASALVSTA IALLLVCLAL LLPAANSEIH 

       310        320        330        340        350        360 
LGVLSIFWGI AMMIIGLGMQ VKVLALAPDA TDVAMALFSG IFNIGIGAGA LVGNQVSLHW 

       370        380        390 
SMSMIGYVGA VPAFAALIWS IIIFRRWPVT LEEQTQ 

« Hide

References

« Hide 'large scale' references
[1]"A 570-kb DNA sequence of the Escherichia coli K-12 genome corresponding to the 28.0-40.1 min region on the linkage map."
Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K., Itoh T., Kasai H., Kashimoto K., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T., Mizobuchi K., Mori H., Mori T., Motomura K. expand/collapse author list , Nakade S., Nakamura Y., Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y., Sivasundaram S., Tagami H., Takeda J., Takemoto K., Takeuchi Y., Wada C., Yamamoto Y., Horiuchi T.
DNA Res. 3:363-377(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[2]"The complete genome sequence of Escherichia coli K-12."
Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V., Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F., Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B., Shao Y.
Science 277:1453-1462(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / MG1655 / ATCC 47076.
[3]"Highly accurate genome sequences of Escherichia coli K-12 strains MG1655 and W3110."
Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S., Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.
Mol. Syst. Biol. 2:E1-E5(2006) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: K12 / W3110 / ATCC 27325 / DSM 5911.
[4]"Genetic and functional analysis of the multiple antibiotic resistance (mar) locus in Escherichia coli."
Cohen S.P., Haechler H., Levy S.B.
J. Bacteriol. 175:1484-1492(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 217-396.
[5]"Transcriptional activation of ydeA, which encodes a member of the major facilitator superfamily, interferes with arabinose accumulation and induction of the Escherichia coli arabinose PBAD promoter."
Bost S., Silva F., Belin D.
J. Bacteriol. 181:2185-2191(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: SB0.
[6]"Escherichia coli gene ydeA encodes a major facilitator pump which exports L-arabinose and isopropyl-beta-D-thiogalactopyranoside."
Carole S., Pichoff S., Bouche J.-P.
J. Bacteriol. 181:5123-5125(1999) [PubMed] [Europe PMC] [Abstract]
Cited for: CHARACTERIZATION.
Strain: JS219.
[7]"Global topology analysis of the Escherichia coli inner membrane proteome."
Daley D.O., Rapp M., Granseth E., Melen K., Drew D., von Heijne G.
Science 308:1321-1323(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: TOPOLOGY [LARGE SCALE ANALYSIS].
Strain: K12 / MG1655 / ATCC 47076.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U00096 Genomic DNA. Translation: AAC74601.1.
AP009048 Genomic DNA. Translation: BAA15210.1.
M96235 Genomic DNA. No translation available.
PIRC64907.
RefSeqNP_416045.1. NC_000913.3.
YP_489791.1. NC_007779.1.

3D structure databases

ProteinModelPortalP31122.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING511145.b1528.

Protein family/group databases

TCDB2.A.1.2.15. the major facilitator superfamily (mfs).

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaAAC74601; AAC74601; b1528.
BAA15210; BAA15210; BAA15210.
GeneID12930477.
947218.
KEGGecj:Y75_p1503.
eco:b1528.
PATRIC32118354. VBIEscCol129921_1597.

Organism-specific databases

EchoBASEEB1592.
EcoGeneEG11636. sotB.

Phylogenomic databases

eggNOGCOG2814.
HOGENOMHOG000268498.
KOK08159.
OMAGNDIRAN.
OrthoDBEOG6TJ7VJ.
PhylomeDBP31122.

Enzyme and pathway databases

BioCycEcoCyc:YDEA-MONOMER.
ECOL316407:JW1521-MONOMER.
MetaCyc:YDEA-MONOMER.

Gene expression databases

GenevestigatorP31122.

Family and domain databases

HAMAPMF_00517. MFS_SotB.
InterProIPR011701. MFS.
IPR020846. MFS_dom.
IPR016196. MFS_dom_general_subst_transpt.
IPR023495. Sugar_effux_transptr_put.
[Graphical view]
PfamPF07690. MFS_1. 1 hit.
[Graphical view]
SUPFAMSSF103473. SSF103473. 1 hit.
PROSITEPS50850. MFS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP31122.

Entry information

Entry nameSOTB_ECOLI
AccessionPrimary (citable) accession number: P31122
Secondary accession number(s): P76883, P77353
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Escherichia coli

Escherichia coli (strain K12): entries and cross-references to EcoGene