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Protein

Bifunctional protein Aas

Gene

aas

Organism
Escherichia coli (strain K12)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Plays a role in lysophospholipid acylation. Transfers fatty acids to the 1-position via an enzyme-bound acyl-ACP intermediate in the presence of ATP and magnesium. Its physiological function is to regenerate phosphatidylethanolamine from 2-acyl-glycero-3-phosphoethanolamine (2-acyl-GPE) formed by transacylation reactions or degradation by phospholipase A1.UniRule annotation1 Publication

Catalytic activityi

Acyl-[acyl-carrier-protein] + O-(2-acyl-sn-glycero-3-phospho)ethanolamine = [acyl-carrier-protein] + O-(1-beta-acyl-2-acyl-sn-glycero-3-phospho)ethanolamine.UniRule annotation
ATP + a long-chain fatty acid + an [acyl-carrier-protein] = AMP + diphosphate + a long-chain acyl-[acyl-carrier-protein].UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei36 – 361

GO - Molecular functioni

  • acyl-[acyl-carrier-protein]-phospholipid O-acyltransferase activity Source: EcoliWiki
  • ATP binding Source: UniProtKB-KW
  • long-chain fatty acid [acyl-carrier-protein] ligase activity Source: EcoliWiki

GO - Biological processi

  • fatty acid metabolic process Source: EcoliWiki
  • phospholipid biosynthetic process Source: EcoliWiki
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Ligase, Transferase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciEcoCyc:AAS-MONOMER.
ECOL316407:JW2804-MONOMER.
MetaCyc:AAS-MONOMER.
BRENDAi2.3.1.40. 2026.

Protein family/group databases

TCDBi4.C.1.1.16. the proposed fatty acid transporter (fat) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional protein AasUniRule annotation
Including the following 2 domains:
2-acylglycerophosphoethanolamine acyltransferaseUniRule annotation (EC:2.3.1.40UniRule annotation)
Alternative name(s):
2-acyl-GPE acyltransferaseUniRule annotation
Acyl-[acyl-carrier-protein]--phospholipid O-acyltransferaseUniRule annotation
Acyl-[acyl-carrier-protein] synthetaseUniRule annotation (EC:6.2.1.20UniRule annotation)
Alternative name(s):
Acyl-ACP synthetaseUniRule annotation
Long-chain-fatty-acid--[acyl-carrier-protein] ligaseUniRule annotation
Gene namesi
Name:aasUniRule annotation
Ordered Locus Names:b2836, JW2804
OrganismiEscherichia coli (strain K12)
Taxonomic identifieri83333 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeEscherichia
Proteomesi
  • UP000000318 Componenti: Chromosome
  • UP000000625 Componenti: Chromosome

Organism-specific databases

EcoGeneiEG11679. aas.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei258 – 27720HelicalUniRule annotationAdd
BLAST
Transmembranei409 – 43325HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  • integral component of membrane Source: EcoliWiki
  • plasma membrane Source: EcoCyc
Complete GO annotation...

Keywords - Cellular componenti

Cell inner membrane, Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi36 – 361H → A: Loss of 2-acyl-GPE acyltransferase activity; retains acyl-ACP synthetase activity. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 719719Bifunctional protein AasPRO_0000193046Add
BLAST

Proteomic databases

PaxDbiP31119.

Interactioni

Protein-protein interaction databases

BioGridi4259527. 15 interactions.
DIPiDIP-9025N.
IntActiP31119. 9 interactions.
MINTiMINT-1308806.
STRINGi511145.b2836.

Structurei

3D structure databases

ProteinModelPortaliP31119.
SMRiP31119. Positions 233-701.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni15 – 138124AcyltransferaseAdd
BLAST
Regioni233 – 646414AMP-bindingAdd
BLAST

Sequence similaritiesi

In the N-terminal section; belongs to the 2-acyl-GPE acetyltransferase family.UniRule annotation
In the C-terminal section; belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0204. LUCA.
COG0318. LUCA.
HOGENOMiHOG000004907.
InParanoidiP31119.
KOiK05939.
OMAiANWVYLE.
PhylomeDBiP31119.

Family and domain databases

HAMAPiMF_01162. Aas. 1 hit.
InterProiIPR023775. Aas.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF00501. AMP-binding. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31119-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MLFSFFRNLC RVLYRVRVTG DTQALKGERV LITPNHVSFI DGILLGLFLP
60 70 80 90 100
VRPVFAVYTS ISQQWYMRWL KSFIDFVPLD PTQPMAIKHL VRLVEQGRPV
110 120 130 140 150
VIFPEGRITT TGSLMKIYDG AGFVAAKSGA TVIPVRIEGA ELTHFSRLKG
160 170 180 190 200
LVKRRLFPQI TLHILPPTQV AMPDAPRARD RRKIAGEMLH QIMMEARMAV
210 220 230 240 250
RPRETLYESL LSAMYRFGAG KKCVEDVNFT PDSYRKLLTK TLFVGRILEK
260 270 280 290 300
YSVEGERIGL MLPNAGISAA VIFGAIARRR MPAMMNYTAG VKGLTSAITA
310 320 330 340 350
AEIKTIFTSR QFLDKGKLWH LPEQLTQVRW VYLEDLKADV TTADKVWIFA
360 370 380 390 400
HLLMPRLAQV KQQPEEEALI LFTSGSEGHP KGVVHSHKSI LANVEQIKTI
410 420 430 440 450
ADFTTNDRFM SALPLFHSFG LTVGLFTPLL TGAEVFLYPS PLHYRIVPEL
460 470 480 490 500
VYDRSCTVLF GTSTFLGHYA RFANPYDFYR LRYVVAGAEK LQESTKQLWQ
510 520 530 540 550
DKFGLRILEG YGVTECAPVV SINVPMAAKP GTVGRILPGM DARLLSVPGI
560 570 580 590 600
EEGGRLQLKG PNIMNGYLRV EKPGVLEVPT AENVRGEMER GWYDTGDIVR
610 620 630 640 650
FDEQGFVQIQ GRAKRFAKIA GEMVSLEMVE QLALGVSPDK VHATAIKSDA
660 670 680 690 700
SKGEALVLFT TDNELTRDKL QQYAREHGVP ELAVPRDIRY LKQMPLLGSG
710
KPDFVTLKSW VDEAEQHDE
Length:719
Mass (Da):80,700
Last modified:November 1, 1997 - v2
Checksum:iF4F1021E57835EB2
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti15 – 162RV → AL in AAA17550 (PubMed:8300626).Curated
Sequence conflicti202 – 2021P → S in AAA17550 (PubMed:8300626).Curated
Sequence conflicti281 – 2811M → I in AAT42454 (PubMed:12664169).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14681 Unassigned DNA. Translation: AAA17550.1.
U29581 Genomic DNA. Translation: AAB40483.1.
U00096 Genomic DNA. Translation: AAC75875.1.
AP009048 Genomic DNA. Translation: BAE76905.1.
AY625100 Genomic DNA. Translation: AAT42454.1.
PIRiE65066.
RefSeqiNP_417313.1. NC_000913.3.
WP_000899054.1. NZ_LN832404.1.

Genome annotation databases

EnsemblBacteriaiAAC75875; AAC75875; b2836.
BAE76905; BAE76905; BAE76905.
GeneIDi947315.
KEGGiecj:JW2804.
eco:b2836.
PATRICi32121090. VBIEscCol129921_2934.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L14681 Unassigned DNA. Translation: AAA17550.1.
U29581 Genomic DNA. Translation: AAB40483.1.
U00096 Genomic DNA. Translation: AAC75875.1.
AP009048 Genomic DNA. Translation: BAE76905.1.
AY625100 Genomic DNA. Translation: AAT42454.1.
PIRiE65066.
RefSeqiNP_417313.1. NC_000913.3.
WP_000899054.1. NZ_LN832404.1.

3D structure databases

ProteinModelPortaliP31119.
SMRiP31119. Positions 233-701.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi4259527. 15 interactions.
DIPiDIP-9025N.
IntActiP31119. 9 interactions.
MINTiMINT-1308806.
STRINGi511145.b2836.

Protein family/group databases

TCDBi4.C.1.1.16. the proposed fatty acid transporter (fat) family.

Proteomic databases

PaxDbiP31119.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAC75875; AAC75875; b2836.
BAE76905; BAE76905; BAE76905.
GeneIDi947315.
KEGGiecj:JW2804.
eco:b2836.
PATRICi32121090. VBIEscCol129921_2934.

Organism-specific databases

EchoBASEiEB1630.
EcoGeneiEG11679. aas.

Phylogenomic databases

eggNOGiENOG4105CEY. Bacteria.
COG0204. LUCA.
COG0318. LUCA.
HOGENOMiHOG000004907.
InParanoidiP31119.
KOiK05939.
OMAiANWVYLE.
PhylomeDBiP31119.

Enzyme and pathway databases

BioCyciEcoCyc:AAS-MONOMER.
ECOL316407:JW2804-MONOMER.
MetaCyc:AAS-MONOMER.
BRENDAi2.3.1.40. 2026.

Miscellaneous databases

PROiP31119.

Family and domain databases

HAMAPiMF_01162. Aas. 1 hit.
InterProiIPR023775. Aas.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
IPR002123. Plipid/glycerol_acylTrfase.
[Graphical view]
PfamiPF01553. Acyltransferase. 1 hit.
PF00501. AMP-binding. 1 hit.
[Graphical view]
SMARTiSM00563. PlsC. 1 hit.
[Graphical view]
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiAAS_ECOLI
AccessioniPrimary (citable) accession number: P31119
Secondary accession number(s): Q2MA01, Q46935, Q6IU49
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: November 1, 1997
Last modified: September 7, 2016
This is version 132 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Multifunctional enzyme, Reference proteome

Documents

  1. Escherichia coli
    Escherichia coli (strain K12): entries and cross-references to EcoGene
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.