P31118 (MTC2_PBCV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase CviAII
Short name=M.CviAII
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase CviAII

Gene names

Name:	CVIAIIM
Ordered Locus Names:	A251R

Organism

Paramecium bursaria Chlorella virus 1 (PBCV-1)

Taxonomic identifier

10506 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus

Virus host

Chlorella [TaxID: 114049]

Protein attributes

Sequence length	326 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the CviAII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 326	326	Modification methylase CviAII		PRO_0000087950

Sequences

	Sequence		Length	Mass (Da)
	P31118 [UniParc]. Last modified December 15, 1998. Version 2. Checksum: 8CC1FC75D85F0D89	FASTA	326	37,492
	10 20 30 40 50 60 MNRIGYIGSK LKLKDWIFEE ISKRTDDTYT KFADLFAGSC IMTHEALEKK YECISNDLET 70 80 90 100 110 120 YSYVIMNGLK CPFSDKLQNI IETLDDLDTK DMVIPGFVTL TYSPRGNRMY FTEDIAMRID 130 140 150 160 170 180 IIRENIERMK ERVSTDEYNF LLASLLTSAD SVKNTSVVYG AYLKKFKKTA LKRMVFAPLH 190 200 210 220 230 240 TRSTTVTLET FNEDATELEI KTDIAYVDPP YNSRQYGANY FVLNQILTPK EIGNGVTGLP 250 260 270 280 290 300 EYKKSSFCRK QEVAMSFHKM LKNVSARLFV ISYSSESLLS KGDMVALLSQ YGKCEVVVRN 310 320 HKRFKAQISA VGNDVEEYLF FVYIEQ

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system." Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L. Nucleic Acids Res. 20:5351-5356(1992) [PubMed: 1437552] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genome." Lu Z., Li Y., Zhang Y., Kutish G.F., Rock D.L., van Etten J.L. Virology 206:339-352(1995) [PubMed: 7831789] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86639 Genomic DNA. Translation: AAB92381.1. JF411744 Genomic DNA. Translation: AAC96619.1.
PIR	S27901. S35442. T17743.
RefSeq	NP_048600.2. NC_000852.5.
3D structure databases
ProteinModelPortal	P31118.
ModBase	Search...
Protein family/group databases
REBASE	3542. M.CviAII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	918436.
Phylogenomic databases
ProtClustDB	CLSP2510560.
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR012327. MeTrfase_D12. [Graphical view]
Pfam	PF02086. MethyltransfD12. 1 hit. [Graphical view]
PRINTS	PR00505. D12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTC2_PBCV1

Accession

Primary (citable) accession number: P31118
Secondary accession number(s): Q84569

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	December 15, 1998
Last modified:	October 19, 2011
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program