Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P31118

- MTC2_PBCV1

UniProt

P31118 - MTC2_PBCV1

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein

Modification methylase CviAII

Gene

CVIAIIM

Organism
Paramecium bursaria Chlorella virus 1 (PBCV-1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the CviAII endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3542. M.CviAII.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase CviAII (EC:2.1.1.72)
Short name:
M.CviAII
Alternative name(s):
Adenine-specific methyltransferase CviAII
Gene namesi
Name:CVIAIIM
Ordered Locus Names:A251R
OrganismiParamecium bursaria Chlorella virus 1 (PBCV-1)
Taxonomic identifieri10506 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 114049]
ProteomesiUP000000862: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 326326Modification methylase CviAIIPRO_0000087950Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP31118.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 2 hits.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamiPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSiPR00505. D12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 2 hits.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P31118-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MNRIGYIGSK LKLKDWIFEE ISKRTDDTYT KFADLFAGSC IMTHEALEKK
60 70 80 90 100
YECISNDLET YSYVIMNGLK CPFSDKLQNI IETLDDLDTK DMVIPGFVTL
110 120 130 140 150
TYSPRGNRMY FTEDIAMRID IIRENIERMK ERVSTDEYNF LLASLLTSAD
160 170 180 190 200
SVKNTSVVYG AYLKKFKKTA LKRMVFAPLH TRSTTVTLET FNEDATELEI
210 220 230 240 250
KTDIAYVDPP YNSRQYGANY FVLNQILTPK EIGNGVTGLP EYKKSSFCRK
260 270 280 290 300
QEVAMSFHKM LKNVSARLFV ISYSSESLLS KGDMVALLSQ YGKCEVVVRN
310 320
HKRFKAQISA VGNDVEEYLF FVYIEQ
Length:326
Mass (Da):37,492
Last modified:December 15, 1998 - v2
Checksum:i8CC1FC75D85F0D89
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M86639 Genomic DNA. Translation: AAB92381.1.
JF411744 Genomic DNA. Translation: AAC96619.1.
PIRiS35442. S27901.
T17743.
RefSeqiNP_048600.2. NC_000852.5.

Genome annotation databases

GeneIDi918436.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
M86639 Genomic DNA. Translation: AAB92381.1 .
JF411744 Genomic DNA. Translation: AAC96619.1 .
PIRi S35442. S27901.
T17743.
RefSeqi NP_048600.2. NC_000852.5.

3D structure databases

ProteinModelPortali P31118.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 3542. M.CviAII.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 918436.

Family and domain databases

Gene3Di 3.40.50.150. 2 hits.
InterProi IPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view ]
Pfami PF02086. MethyltransfD12. 1 hit.
[Graphical view ]
PRINTSi PR00505. D12N6MTFRASE.
SUPFAMi SSF53335. SSF53335. 2 hits.
PROSITEi PS00092. N6_MTASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system."
    Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L.
    Nucleic Acids Res. 20:5351-5356(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genome."
    Lu Z., Li Y., Zhang Y., Kutish G.F., Rock D.L., van Etten J.L.
    Virology 206:339-352(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.

Entry informationi

Entry nameiMTC2_PBCV1
AccessioniPrimary (citable) accession number: P31118
Secondary accession number(s): Q84569
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 15, 1998
Last modified: October 1, 2014
This is version 70 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3