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P31118

- MTC2_PBCV1

UniProt

P31118 - MTC2_PBCV1

Protein

Modification methylase CviAII

Gene

CVIAIIM

Organism
Paramecium bursaria Chlorella virus 1 (PBCV-1)
Status
Reviewed - Annotation score: 2 out of 5- Protein inferred from homologyi
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    • History
      Entry version 70 (01 Oct 2014)
      Sequence version 2 (15 Dec 1998)
      Previous versions | rss
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    Functioni

    This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the CviAII endonuclease.

    Catalytic activityi

    S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

    GO - Molecular functioni

    1. nucleic acid binding Source: InterPro
    2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Methyltransferase, Transferase

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    S-adenosyl-L-methionine

    Protein family/group databases

    REBASEi3542. M.CviAII.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Modification methylase CviAII (EC:2.1.1.72)
    Short name:
    M.CviAII
    Alternative name(s):
    Adenine-specific methyltransferase CviAII
    Gene namesi
    Name:CVIAIIM
    Ordered Locus Names:A251R
    OrganismiParamecium bursaria Chlorella virus 1 (PBCV-1)
    Taxonomic identifieri10506 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
    Virus hostiChlorella [TaxID: 114049]
    ProteomesiUP000000862: Genome

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 326326Modification methylase CviAIIPRO_0000087950Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP31118.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the N(4)/N(6)-methyltransferase family.Curated

    Family and domain databases

    Gene3Di3.40.50.150. 2 hits.
    InterProiIPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view]
    PfamiPF02086. MethyltransfD12. 1 hit.
    [Graphical view]
    PRINTSiPR00505. D12N6MTFRASE.
    SUPFAMiSSF53335. SSF53335. 2 hits.
    PROSITEiPS00092. N6_MTASE. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P31118-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNRIGYIGSK LKLKDWIFEE ISKRTDDTYT KFADLFAGSC IMTHEALEKK    50
    YECISNDLET YSYVIMNGLK CPFSDKLQNI IETLDDLDTK DMVIPGFVTL 100
    TYSPRGNRMY FTEDIAMRID IIRENIERMK ERVSTDEYNF LLASLLTSAD 150
    SVKNTSVVYG AYLKKFKKTA LKRMVFAPLH TRSTTVTLET FNEDATELEI 200
    KTDIAYVDPP YNSRQYGANY FVLNQILTPK EIGNGVTGLP EYKKSSFCRK 250
    QEVAMSFHKM LKNVSARLFV ISYSSESLLS KGDMVALLSQ YGKCEVVVRN 300
    HKRFKAQISA VGNDVEEYLF FVYIEQ 326
    Length:326
    Mass (Da):37,492
    Last modified:December 15, 1998 - v2
    Checksum:i8CC1FC75D85F0D89
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86639 Genomic DNA. Translation: AAB92381.1.
    JF411744 Genomic DNA. Translation: AAC96619.1.
    PIRiS35442. S27901.
    T17743.
    RefSeqiNP_048600.2. NC_000852.5.

    Genome annotation databases

    GeneIDi918436.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M86639 Genomic DNA. Translation: AAB92381.1 .
    JF411744 Genomic DNA. Translation: AAC96619.1 .
    PIRi S35442. S27901.
    T17743.
    RefSeqi NP_048600.2. NC_000852.5.

    3D structure databases

    ProteinModelPortali P31118.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 3542. M.CviAII.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 918436.

    Family and domain databases

    Gene3Di 3.40.50.150. 2 hits.
    InterProi IPR002052. DNA_methylase_N6_adenine_CS.
    IPR012327. MeTrfase_D12.
    IPR029063. SAM-dependent_MTases-like.
    [Graphical view ]
    Pfami PF02086. MethyltransfD12. 1 hit.
    [Graphical view ]
    PRINTSi PR00505. D12N6MTFRASE.
    SUPFAMi SSF53335. SSF53335. 2 hits.
    PROSITEi PS00092. N6_MTASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system."
      Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L.
      Nucleic Acids Res. 20:5351-5356(1992) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genome."
      Lu Z., Li Y., Zhang Y., Kutish G.F., Rock D.L., van Etten J.L.
      Virology 206:339-352(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.

    Entry informationi

    Entry nameiMTC2_PBCV1
    AccessioniPrimary (citable) accession number: P31118
    Secondary accession number(s): Q84569
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: July 1, 1993
    Last sequence update: December 15, 1998
    Last modified: October 1, 2014
    This is version 70 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3