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P31118 (MTC2_PBCV1) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 69. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Modification methylase CviAII

Short name=M.CviAII
EC=2.1.1.72
Alternative name(s):
Adenine-specific methyltransferase CviAII
Gene names
Name:CVIAIIM
Ordered Locus Names:A251R
OrganismParamecium bursaria Chlorella virus 1 (PBCV-1) [Reference proteome]
Taxonomic identifier10506 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostChlorella [TaxID: 114049]

Protein attributes

Sequence length326 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

This methylase recognizes the double-stranded sequence CATG, causes specific methylation on A-2 on both strands and protects the DNA from cleavage by the CviAII endonuclease.

Catalytic activity

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

Sequence similarities

Belongs to the N(4)/N(6)-methyltransferase family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 326326Modification methylase CviAII
PRO_0000087950

Sequences

Sequence LengthMass (Da)Tools
P31118 [UniParc].

Last modified December 15, 1998. Version 2.
Checksum: 8CC1FC75D85F0D89

FASTA32637,492
        10         20         30         40         50         60 
MNRIGYIGSK LKLKDWIFEE ISKRTDDTYT KFADLFAGSC IMTHEALEKK YECISNDLET 

        70         80         90        100        110        120 
YSYVIMNGLK CPFSDKLQNI IETLDDLDTK DMVIPGFVTL TYSPRGNRMY FTEDIAMRID 

       130        140        150        160        170        180 
IIRENIERMK ERVSTDEYNF LLASLLTSAD SVKNTSVVYG AYLKKFKKTA LKRMVFAPLH 

       190        200        210        220        230        240 
TRSTTVTLET FNEDATELEI KTDIAYVDPP YNSRQYGANY FVLNQILTPK EIGNGVTGLP 

       250        260        270        280        290        300 
EYKKSSFCRK QEVAMSFHKM LKNVSARLFV ISYSSESLLS KGDMVALLSQ YGKCEVVVRN 

       310        320 
HKRFKAQISA VGNDVEEYLF FVYIEQ 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system."
Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L.
Nucleic Acids Res. 20:5351-5356(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of 45 kb of DNA located at the left end of the chlorella virus PBCV-1 genome."
Lu Z., Li Y., Zhang Y., Kutish G.F., Rock D.L., van Etten J.L.
Virology 206:339-352(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], SEQUENCE REVISION.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86639 Genomic DNA. Translation: AAB92381.1.
JF411744 Genomic DNA. Translation: AAC96619.1.
PIRS27901. S35442.
T17743.
RefSeqNP_048600.2. NC_000852.5.

3D structure databases

ProteinModelPortalP31118.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE3542. M.CviAII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID918436.

Family and domain databases

Gene3D3.40.50.150. 2 hits.
InterProIPR002052. DNA_methylase_N6_adenine_CS.
IPR012327. MeTrfase_D12.
IPR029063. SAM-dependent_MTases-like.
[Graphical view]
PfamPF02086. MethyltransfD12. 1 hit.
[Graphical view]
PRINTSPR00505. D12N6MTFRASE.
SUPFAMSSF53335. SSF53335. 2 hits.
PROSITEPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameMTC2_PBCV1
AccessionPrimary (citable) accession number: P31118
Secondary accession number(s): Q84569
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: December 15, 1998
Last modified: June 11, 2014
This is version 69 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries