Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Type-2 restriction enzyme CviAII

Gene

CVIAIIR

Organism
Paramecium bursaria Chlorella virus 1 (PBCV-1)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein predictedi

Functioni

Recognizes the double-stranded sequence CATG and cleaves after C-1.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Protein family/group databases

REBASEi2211. CviAII.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme CviAII (EC:3.1.21.4)
Short name:
R.CviAII
Alternative name(s):
Endonuclease CviAII
Type II restriction enzyme CviAII
Gene namesi
Name:CVIAIIR
Ordered Locus Names:A252R
OrganismiParamecium bursaria Chlorella virus 1 (PBCV-1)
Taxonomic identifieri10506 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 114049]
ProteomesiUP000000862: Genome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 342342Type-2 restriction enzyme CviAIIPRO_0000077297Add
BLAST

Sequencei

Sequence statusi: Complete.

P31117-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTQKILNPVT GRFVKVDGST GKKIKTGNIY DVNNILSSKL TKKIFDKIRR
60 70 80 90 100
NDLAKEERET QQLTKKISKG IFDKIRSENK KYEKNHQKLT KKMVLDIFSK
110 120 130 140 150
IYKEDSRKSK TQCVVSEKKD NGGVLLTEDL GKIFEKSICM LYDTPYIGPY
160 170 180 190 200
KYGNEKPMLL KTRLHKLLDF FPELTHTAAG GALHDFTTKN SRYLSAKTSK
210 220 230 240 250
KKDGKVAPQK IGQPTKKKFL EFFNLPPDTS NDDIKLFIKK NIVRILDEYF
260 270 280 290 300
KYTFDDTIIY YNEVNNIIML VKTLKKVKFD PNLIEFGCNK PGKSWKESTI
310 320 330 340
LFYNNKRLGE FQIHTSRSCI KFRWFFENIL LLFPDNFEVT IL
Length:342
Mass (Da):39,928
Last modified:July 1, 1993 - v1
Checksum:iD553D28BD6300522
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86639 Genomic DNA. Translation: AAB92382.1.
JF411744 Genomic DNA. Translation: AAC96620.1.
PIRiS35443. S27902.
RefSeqiNP_048602.2. NC_000852.5.

Genome annotation databases

GeneIDi918443.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M86639 Genomic DNA. Translation: AAB92382.1.
JF411744 Genomic DNA. Translation: AAC96620.1.
PIRiS35443. S27902.
RefSeqiNP_048602.2. NC_000852.5.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi2211. CviAII.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi918443.

Family and domain databases

ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system."
    Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L.
    Nucleic Acids Res. 20:5351-5356(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Analysis of 94 kb of the chlorella virus PBCV-1 330-kb genome: map positions 88 to 182."
    Lu Z., Li Y., Que Q., Kutish G.F., Rock D.L., van Etten J.L.
    Virology 216:102-123(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiT2C2_PBCV1
AccessioniPrimary (citable) accession number: P31117
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.