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P31117 (T2C2_PBCV1) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 61. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme CviAII

Short name=R.CviAII
EC=3.1.21.4
Alternative name(s):
Endonuclease CviAII
Type II restriction enzyme CviAII
Gene names
Name:CVIAIIR
Ordered Locus Names:A252R
OrganismParamecium bursaria Chlorella virus 1 (PBCV-1) [Reference proteome]
Taxonomic identifier10506 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostChlorella [TaxID: 114049]

Protein attributes

Sequence length342 AA.
Sequence statusComplete.
Protein existencePredicted

General annotation (Comments)

Function

Recognizes the double-stranded sequence CATG and cleaves after C-1.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
   Biological processRestriction system
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 342342Type-2 restriction enzyme CviAII
PRO_0000077297

Sequences

Sequence LengthMass (Da)Tools
P31117 [UniParc].

Last modified July 1, 1993. Version 1.
Checksum: D553D28BD6300522

FASTA34239,928
        10         20         30         40         50         60 
MTQKILNPVT GRFVKVDGST GKKIKTGNIY DVNNILSSKL TKKIFDKIRR NDLAKEERET 

        70         80         90        100        110        120 
QQLTKKISKG IFDKIRSENK KYEKNHQKLT KKMVLDIFSK IYKEDSRKSK TQCVVSEKKD 

       130        140        150        160        170        180 
NGGVLLTEDL GKIFEKSICM LYDTPYIGPY KYGNEKPMLL KTRLHKLLDF FPELTHTAAG 

       190        200        210        220        230        240 
GALHDFTTKN SRYLSAKTSK KKDGKVAPQK IGQPTKKKFL EFFNLPPDTS NDDIKLFIKK 

       250        260        270        280        290        300 
NIVRILDEYF KYTFDDTIIY YNEVNNIIML VKTLKKVKFD PNLIEFGCNK PGKSWKESTI 

       310        320        330        340 
LFYNNKRLGE FQIHTSRSCI KFRWFFENIL LLFPDNFEVT IL 

« Hide

References

« Hide 'large scale' references
[1]"Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system."
Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L.
Nucleic Acids Res. 20:5351-5356(1992) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Analysis of 94 kb of the chlorella virus PBCV-1 330-kb genome: map positions 88 to 182."
Lu Z., Li Y., Que Q., Kutish G.F., Rock D.L., van Etten J.L.
Virology 216:102-123(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M86639 Genomic DNA. Translation: AAB92382.1.
JF411744 Genomic DNA. Translation: AAC96620.1.
PIRS27902. S35443.
RefSeqNP_048602.2. NC_000852.5.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE2211. CviAII.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID918443.

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2C2_PBCV1
AccessionPrimary (citable) accession number: P31117
Entry history
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: April 16, 2014
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries