P31117 (T2C2_PBCV1) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Type-2 restriction enzyme CviAII
Short name=R.CviAII
EC=3.1.21.4

Alternative name(s):
Endonuclease CviAII
Type II restriction enzyme CviAII

Gene names

Name:	CVIAIIR
Ordered Locus Names:	A252R

Organism

Paramecium bursaria Chlorella virus 1 (PBCV-1) [Reference proteome]

Taxonomic identifier

10506 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus

Virus host

Chlorella [TaxID: 114049]

Protein attributes

Sequence length	342 AA.
Sequence status	Complete.
Protein existence	Predicted

General annotation (Comments)

Function	Recognizes the double-stranded sequence CATG and cleaves after C-1.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Ontologies

Keywords
Biological process	Restriction system
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW nucleic acid phosphodiester bond hydrolysis Inferred from electronic annotation. Source: GOC
Molecular_function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 342	342	Type-2 restriction enzyme CviAII		PRO_0000077297

Sequences

	Sequence		Length	Mass (Da)
	P31117 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: D553D28BD6300522	FASTA	342	39,928
	10 20 30 40 50 60 MTQKILNPVT GRFVKVDGST GKKIKTGNIY DVNNILSSKL TKKIFDKIRR NDLAKEERET 70 80 90 100 110 120 QQLTKKISKG IFDKIRSENK KYEKNHQKLT KKMVLDIFSK IYKEDSRKSK TQCVVSEKKD 130 140 150 160 170 180 NGGVLLTEDL GKIFEKSICM LYDTPYIGPY KYGNEKPMLL KTRLHKLLDF FPELTHTAAG 190 200 210 220 230 240 GALHDFTTKN SRYLSAKTSK KKDGKVAPQK IGQPTKKKFL EFFNLPPDTS NDDIKLFIKK 250 260 270 280 290 300 NIVRILDEYF KYTFDDTIIY YNEVNNIIML VKTLKKVKFD PNLIEFGCNK PGKSWKESTI 310 320 330 340 LFYNNKRLGE FQIHTSRSCI KFRWFFENIL LLFPDNFEVT IL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Characterization of Chlorella virus PBCV-1 CviAII restriction and modification system." Zhang Y., Nelson M., Nietfeldt J.W., Burbank D.E., van Etten J.L. Nucleic Acids Res. 20:5351-5356(1992) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of 94 kb of the chlorella virus PBCV-1 330-kb genome: map positions 88 to 182." Lu Z., Li Y., Que Q., Kutish G.F., Rock D.L., van Etten J.L. Virology 216:102-123(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M86639 Genomic DNA. Translation: AAB92382.1. JF411744 Genomic DNA. Translation: AAC96620.1.
PIR	S27902. S35443.
RefSeq	NP_048602.2. NC_000852.5.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	2211. CviAII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	918443.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2C2_PBCV1

Accession

Primary (citable) accession number: P31117

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	April 3, 2013
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program