P31115 (PUS3_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
tRNA pseudouridine(38/39) synthase
EC=5.4.99.45

Alternative name(s):
Depressed growth-rate protein DEG1
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3

Gene names

Name:	DEG1
Synonyms:	HRM3, PUS3
Ordered Locus Names:	YFL001W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	442 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Formation of pseudouridines at positions 38 and 39 in the anticodon stem and loop of transfer RNAs.
Catalytic activity	tRNA uridine(38/39) = tRNA pseudouridine(38/39).
Subcellular location	Nucleus.
Miscellaneous	Present with 5860 molecules/cell in log phase SD medium. Ref.7
Sequence similarities	Belongs to the tRNA pseudouridine synthase TruA family.

Ontologies

Keywords
Biological process	tRNA processing
Cellular component	Nucleus
Molecular function	Isomerase
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	tRNA pseudouridine synthesis Inferred from direct assay Ref.6. Source: SGD
Cellular component	cytoplasm Inferred from direct assay Ref.6. Source: SGD nucleus Inferred from direct assay Ref.6. Source: SGD
Molecular function	RNA binding Inferred from electronic annotation. Source: InterPro pseudouridine synthase activity Inferred from direct assay Ref.6. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 442	442	tRNA pseudouridine(38/39) synthase		PRO_0000057519
Sites
	Active site	151	1	Nucleophile By similarity
	Binding site	222	1	Substrate By similarity
Amino acid modifications
	Modified residue	68	1	Phosphoserine Ref.8

Sequences

	Sequence		Length	Mass (Da)
	P31115 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 89C35917366BDB9B	FASTA	442	50,889
	10 20 30 40 50 60 MSNFIRRLVG KMKAISTGTN AIVSKKDSIY ANWSKEQLIR RITELENANK PHSEKFQHIE 70 80 90 100 110 120 DNKKRKISQE EVTRSKAKKA PKKFDFSKHN TRFIALRFAY LGWNYNGLAV QKEYTPLPTV 130 140 150 160 170 180 EGTILEAMNK CKLVPSMVLQ DYKFSRCGRT DKGVSAMNQV ISLEVRSNLT DEEQRDPTND 190 200 210 220 230 240 SREIPYVHVL NQLLPDDIRI SAVCLRPPPN FDARFSCVHR HYKYIFNGKN LNIEKMSKAA 250 260 270 280 290 300 SYFVGERDFR NFCKLDGSKQ ITNFKRTIIS SKILPLSETF YCFDLVGSAF LWHQVRCMMA 310 320 330 340 350 360 ILFLVGQSLE VPEIVLRLTD IEKTPQRPVY EMANDIPLLL YDCKFPEMDW QEPTVDDYKA 370 380 390 400 410 420 IKFTTATEAL TLHYELKAAV CNIFKDVLPT ANTNNFSKTI INLGDGRGKV VGTYVKLEDR 430 440 SVMEPVEVVN AKYSKKKNNK NK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"A gene tightly linked to CEN6 is important for growth of Saccharomyces cerevisiae." Agostoni Carbone M.L., Solinas M., Sora S., Panzeri L. Curr. Genet. 19:1-8(1991) [PubMed: 2036682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Analysis of the nucleotide sequence of chromosome VI from Saccharomyces cerevisiae." Murakami Y., Naitou M., Hagiwara H., Shibata T., Ozawa M., Sasanuma S., Sasanuma M., Tsuchiya Y., Soeda E., Yokoyama K., Yamazaki M., Tashiro H., Eki T. Nat. Genet. 10:261-268(1995) [PubMed: 7670463] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[3]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[4]	"Sequencing of a 23 kb fragment from Saccharomyces cerevisiae chromosome VI." Naitou M., Ozawa M., Sasanuma S., Kobayashi M., Hagiwara H., Shibata T., Hanaoka F., Watanabe K., Ono A., Yamazaki M., Tashiro H., Eki T., Murakami Y. Yeast 12:77-84(1996) [PubMed: 8789262] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[5]	"Approaching a complete repository of sequence-verified protein-encoding clones for Saccharomyces cerevisiae." Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F., Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J., Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J. , Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D., LaBaer J. Genome Res. 17:536-543(2007) [PubMed: 17322287] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 204508 / S288c.
[6]	"Characterization of yeast protein Deg1 as pseudouridine synthase (Pus3) catalyzing the formation of psi 38 and psi 39 in tRNA anticodon loop." Lecointe F., Simos G., Sauer A., Hurt E.C., Motorin Y., Grosjean H. J. Biol. Chem. 273:1316-1323(1998) [PubMed: 9430663] [Abstract] Cited for: FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY.
[7]	"Global analysis of protein expression in yeast." Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N., O'Shea E.K., Weissman J.S. Nature 425:737-741(2003) [PubMed: 14562106] [Abstract] Cited for: LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
[8]	"A multidimensional chromatography technology for in-depth phosphoproteome analysis." Albuquerque C.P., Smolka M.B., Payne S.H., Bafna V., Eng J., Zhou H. Mol. Cell. Proteomics 7:1389-1396(2008) [PubMed: 18407956] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-68, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D50617 Genomic DNA. Translation: BAA09239.1. AY693197 Genomic DNA. Translation: AAT93216.1. BK006940 Genomic DNA. Translation: DAA12440.1.
PIR	S14145.
RefSeq	NP_116655.1. NM_001179965.1.
3D structure databases
ProteinModelPortal	P31115.
SMR	P31115. Positions 91-347.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-5151N.
IntAct	P31115. 6 interactions.
MINT	MINT-531752.
STRING	P31115.
Proteomic databases
PeptideAtlas	P31115.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblFungi	YFL001W; YFL001W; YFL001W.
GeneID	850550.
KEGG	sce:YFL001W.
NMPDR	fig|4932.3.peg.2286.
Organism-specific databases
CYGD	YFL001w.
SGD	S000001895. PUS3.
Phylogenomic databases
eggNOG	fuNOG05410.
GeneTree	EFGT00050000004103.
HOGENOM	HBG594279.
OMA	AVCLRPP.
OrthoDB	EOG46T69C.
Enzyme and pathway databases
BRENDA	5.4.99.12. 984.
Gene expression databases
ArrayExpress	P31115.
Genevestigator	P31115.
GermOnline	YFL001W. Saccharomyces cerevisiae.
Family and domain databases
InterPro	IPR020103. PsdUridine_synth_cat_dom. IPR001406. PsdUridine_synth_TruA. IPR020097. PsdUridine_synth_TruA_a/b_dom. IPR020095. PsdUridine_synth_TruA_C. IPR020094. PsdUridine_synth_TruA_N. [Graphical view]
Gene3D	G3DSA:3.30.70.580. PseudoU_synth_1. 1 hit. G3DSA:3.30.70.660. PseudoU_synth_1. 1 hit.
KO	K01855.
PANTHER	PTHR11142. PseudoU_synth_1. 1 hit.
Pfam	PF01416. PseudoU_synth_1. 2 hits. [Graphical view]
SUPFAM	SSF55120. PsdUridine_synth_cat_dom. 1 hit.
TIGRFAMs	TIGR00071. HisT_truA. 1 hit.
ProtoNet	Search...
Other
NextBio	966327.

Entry information

Entry name

PUS3_YEAST

Accession

Primary (citable) accession number: P31115
Secondary accession number(s): D6VTN0

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	January 25, 2012
This is version 107 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program