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Protein

tRNA pseudouridine(38/39) synthase

Gene

DEG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Formation of pseudouridines at positions 38 and 39 in the anticodon stem and loop of transfer RNAs.1 Publication

Miscellaneous

Present with 5860 molecules/cell in log phase SD medium.1 Publication

Catalytic activityi

tRNA uridine(38/39) = tRNA pseudouridine(38/39).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei151NucleophileBy similarity1
Binding sitei222SubstrateBy similarity1

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • mRNA pseudouridine synthesis Source: SGD
  • tRNA pseudouridine synthesis Source: SGD

Keywordsi

Molecular functionIsomerase
Biological processtRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30453-MONOMER
BRENDAi5.4.99.12 984
5.4.99.45 984

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(38/39) synthase (EC:5.4.99.45)
Alternative name(s):
Depressed growth-rate protein DEG1
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene namesi
Name:DEG1
Synonyms:HRM3, PUS3
Ordered Locus Names:YFL001W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL001W
SGDiS000001895 DEG1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000575191 – 442tRNA pseudouridine(38/39) synthaseAdd BLAST442

Proteomic databases

MaxQBiP31115
PaxDbiP31115
PRIDEiP31115

PTM databases

iPTMnetiP31115

Interactioni

Protein-protein interaction databases

BioGridi31148408 interactors.
DIPiDIP-5151N
IntActiP31115 11 interactors.
STRINGi4932.YFL001W

Structurei

3D structure databases

ProteinModelPortaliP31115
SMRiP31115
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047444
HOGENOMiHOG000199032
InParanoidiP31115
KOiK01855
OMAiKRVCVDT
OrthoDBiEOG092C4CNP

Family and domain databases

Gene3Di3.30.70.6601 hit
HAMAPiMF_00171 TruA, 1 hit
InterProiView protein in InterPro
IPR020103 PsdUridine_synth_cat_dom_sf
IPR001406 PsdUridine_synth_TruA
IPR020097 PsdUridine_synth_TruA_a/b_dom
IPR020095 PsdUridine_synth_TruA_C
PANTHERiPTHR11142 PTHR11142, 1 hit
PfamiView protein in Pfam
PF01416 PseudoU_synth_1, 1 hit
SUPFAMiSSF55120 SSF55120, 1 hit
TIGRFAMsiTIGR00071 hisT_truA, 1 hit

Sequencei

Sequence statusi: Complete.

P31115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNFIRRLVG KMKAISTGTN AIVSKKDSIY ANWSKEQLIR RITELENANK
60 70 80 90 100
PHSEKFQHIE DNKKRKISQE EVTRSKAKKA PKKFDFSKHN TRFIALRFAY
110 120 130 140 150
LGWNYNGLAV QKEYTPLPTV EGTILEAMNK CKLVPSMVLQ DYKFSRCGRT
160 170 180 190 200
DKGVSAMNQV ISLEVRSNLT DEEQRDPTND SREIPYVHVL NQLLPDDIRI
210 220 230 240 250
SAVCLRPPPN FDARFSCVHR HYKYIFNGKN LNIEKMSKAA SYFVGERDFR
260 270 280 290 300
NFCKLDGSKQ ITNFKRTIIS SKILPLSETF YCFDLVGSAF LWHQVRCMMA
310 320 330 340 350
ILFLVGQSLE VPEIVLRLTD IEKTPQRPVY EMANDIPLLL YDCKFPEMDW
360 370 380 390 400
QEPTVDDYKA IKFTTATEAL TLHYELKAAV CNIFKDVLPT ANTNNFSKTI
410 420 430 440
INLGDGRGKV VGTYVKLEDR SVMEPVEVVN AKYSKKKNNK NK
Length:442
Mass (Da):50,889
Last modified:July 1, 1993 - v1
Checksum:i89C35917366BDB9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA Translation: BAA09239.1
AY693197 Genomic DNA Translation: AAT93216.1
BK006940 Genomic DNA Translation: DAA12440.1
PIRiS14145
RefSeqiNP_116655.1, NM_001179965.1

Genome annotation databases

EnsemblFungiiBAA09239; BAA09239; BAA09239
YFL001W; YFL001W; YFL001W
GeneIDi850550
KEGGisce:YFL001W

Similar proteinsi

Entry informationi

Entry nameiPUS3_YEAST
AccessioniPrimary (citable) accession number: P31115
Secondary accession number(s): D6VTN0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: March 28, 2018
This is version 157 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome