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Protein

tRNA pseudouridine(38/39) synthase

Gene

DEG1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Formation of pseudouridines at positions 38 and 39 in the anticodon stem and loop of transfer RNAs.1 Publication

Catalytic activityi

tRNA uridine(38/39) = tRNA pseudouridine(38/39).1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei151NucleophileBy similarity1
Binding sitei222SubstrateBy similarity1

GO - Molecular functioni

  • pseudouridine synthase activity Source: SGD
  • RNA binding Source: InterPro

GO - Biological processi

  • mRNA pseudouridine synthesis Source: SGD
  • tRNA pseudouridine synthesis Source: SGD
Complete GO annotation...

Keywords - Molecular functioni

Isomerase

Keywords - Biological processi

tRNA processing

Enzyme and pathway databases

BioCyciYEAST:G3O-30453-MONOMER.
BRENDAi5.4.99.12. 984.
5.4.99.45. 984.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA pseudouridine(38/39) synthase (EC:5.4.99.45)
Alternative name(s):
Depressed growth-rate protein DEG1
tRNA pseudouridine synthase 3
tRNA pseudouridylate synthase 3
tRNA-uridine isomerase 3
Gene namesi
Name:DEG1
Synonyms:HRM3, PUS3
Ordered Locus Names:YFL001W
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome VI

Organism-specific databases

EuPathDBiFungiDB:YFL001W.
SGDiS000001895. DEG1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: SGD
  • nucleus Source: SGD
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000575191 – 442tRNA pseudouridine(38/39) synthaseAdd BLAST442

Proteomic databases

MaxQBiP31115.
PRIDEiP31115.

PTM databases

iPTMnetiP31115.

Interactioni

Protein-protein interaction databases

BioGridi31148. 304 interactors.
DIPiDIP-5151N.
IntActiP31115. 6 interactors.
MINTiMINT-531752.

Structurei

3D structure databases

ProteinModelPortaliP31115.
SMRiP31115.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

GeneTreeiENSGT00510000047444.
HOGENOMiHOG000199032.
InParanoidiP31115.
KOiK01855.
OMAiNKCKLVP.
OrthoDBiEOG092C4CNP.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.

Sequencei

Sequence statusi: Complete.

P31115-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSNFIRRLVG KMKAISTGTN AIVSKKDSIY ANWSKEQLIR RITELENANK
60 70 80 90 100
PHSEKFQHIE DNKKRKISQE EVTRSKAKKA PKKFDFSKHN TRFIALRFAY
110 120 130 140 150
LGWNYNGLAV QKEYTPLPTV EGTILEAMNK CKLVPSMVLQ DYKFSRCGRT
160 170 180 190 200
DKGVSAMNQV ISLEVRSNLT DEEQRDPTND SREIPYVHVL NQLLPDDIRI
210 220 230 240 250
SAVCLRPPPN FDARFSCVHR HYKYIFNGKN LNIEKMSKAA SYFVGERDFR
260 270 280 290 300
NFCKLDGSKQ ITNFKRTIIS SKILPLSETF YCFDLVGSAF LWHQVRCMMA
310 320 330 340 350
ILFLVGQSLE VPEIVLRLTD IEKTPQRPVY EMANDIPLLL YDCKFPEMDW
360 370 380 390 400
QEPTVDDYKA IKFTTATEAL TLHYELKAAV CNIFKDVLPT ANTNNFSKTI
410 420 430 440
INLGDGRGKV VGTYVKLEDR SVMEPVEVVN AKYSKKKNNK NK
Length:442
Mass (Da):50,889
Last modified:July 1, 1993 - v1
Checksum:i89C35917366BDB9B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09239.1.
AY693197 Genomic DNA. Translation: AAT93216.1.
BK006940 Genomic DNA. Translation: DAA12440.1.
PIRiS14145.
RefSeqiNP_116655.1. NM_001179965.1.

Genome annotation databases

EnsemblFungiiBAA09239; BAA09239; BAA09239.
YFL001W; YFL001W; YFL001W.
GeneIDi850550.
KEGGisce:YFL001W.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D50617 Genomic DNA. Translation: BAA09239.1.
AY693197 Genomic DNA. Translation: AAT93216.1.
BK006940 Genomic DNA. Translation: DAA12440.1.
PIRiS14145.
RefSeqiNP_116655.1. NM_001179965.1.

3D structure databases

ProteinModelPortaliP31115.
SMRiP31115.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi31148. 304 interactors.
DIPiDIP-5151N.
IntActiP31115. 6 interactors.
MINTiMINT-531752.

PTM databases

iPTMnetiP31115.

Proteomic databases

MaxQBiP31115.
PRIDEiP31115.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblFungiiBAA09239; BAA09239; BAA09239.
YFL001W; YFL001W; YFL001W.
GeneIDi850550.
KEGGisce:YFL001W.

Organism-specific databases

EuPathDBiFungiDB:YFL001W.
SGDiS000001895. DEG1.

Phylogenomic databases

GeneTreeiENSGT00510000047444.
HOGENOMiHOG000199032.
InParanoidiP31115.
KOiK01855.
OMAiNKCKLVP.
OrthoDBiEOG092C4CNP.

Enzyme and pathway databases

BioCyciYEAST:G3O-30453-MONOMER.
BRENDAi5.4.99.12. 984.
5.4.99.45. 984.

Miscellaneous databases

PROiP31115.

Family and domain databases

Gene3Di3.30.70.580. 1 hit.
3.30.70.660. 1 hit.
HAMAPiMF_00171. TruA. 1 hit.
InterProiIPR020103. PsdUridine_synth_cat_dom.
IPR001406. PsdUridine_synth_TruA.
IPR020097. PsdUridine_synth_TruA_a/b_dom.
IPR020095. PsdUridine_synth_TruA_C.
IPR020094. PsdUridine_synth_TruA_N.
[Graphical view]
PANTHERiPTHR11142. PTHR11142. 1 hit.
PfamiPF01416. PseudoU_synth_1. 1 hit.
[Graphical view]
SUPFAMiSSF55120. SSF55120. 1 hit.
TIGRFAMsiTIGR00071. hisT_truA. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiPUS3_YEAST
AccessioniPrimary (citable) accession number: P31115
Secondary accession number(s): D6VTN0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: November 2, 2016
This is version 145 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Miscellaneous

Present with 5860 molecules/cell in log phase SD medium.1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  3. Yeast chromosome VI
    Yeast (Saccharomyces cerevisiae) chromosome VI: entries and gene names

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.