P31109 (SNC1_YEAST) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Synaptobrevin homolog 1

Gene names

Name:	SNC1
Ordered Locus Names:	YAL030W

Organism

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Taxonomic identifier

559292 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Saccharomyces

Protein attributes

Sequence length	117 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	SNC1 and SNC2 are vesicle-targeting proteins essential for normal secretory traffic between the Golgi and the plasma membrane. They may also be involved in vesicle fusion.
Subcellular location	Endomembrane system; Single-pass type IV membrane protein. Note: Post-Golgi vesicle membrane Probable.
Post-translational modification	Palmitoylated by SWF1. Ref.6
Sequence similarities	Belongs to the synaptobrevin family. Contains 1 v-SNARE coiled-coil homology domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Coiled coil Transmembrane Transmembrane helix
PTM	Isopeptide bond Lipoprotein Palmitate Ubl conjugation
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	Golgi to plasma membrane transport Inferred from mutant phenotype. Source: SGD endocytosis Inferred from mutant phenotype. Source: SGD exocytosis Inferred from mutant phenotype. Source: SGD vesicle fusion Inferred from direct assay. Source: SGD
Cellular component	SNARE complex Inferred from direct assay. Source: SGD cellular bud neck Inferred from direct assay. Source: SGD endosome Inferred from direct assay. Source: SGD integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Inferred from direct assay. Source: SGD trans-Golgi network Inferred from direct assay. Source: SGD transport vesicle membrane Inferred from direct assay. Source: SGD
Molecular function	SNAP receptor activity Inferred from direct assay. Source: SGD
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 117

117

Synaptobrevin homolog 1

PRO_0000206744

Regions

Topological domain

1 – 94

94

Cytoplasmic Potential

Transmembrane

95 – 111

17

Helical; Anchor for type IV membrane protein; Potential

Topological domain

112 – 117

6

Vesicular Potential

Domain

28 – 88

61

v-SNARE coiled-coil homology

Amino acid modifications

Lipidation

95

1

S-palmitoyl cysteine Ref.6

Cross-link

63

Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin) Ref.5

Secondary structure

1

.

.

.

117

Helix Strand Turn

Details...

Helix

27 – 85

59

Sequences

	Sequence		Length	Mass (Da)
	P31109 [UniParc]. Last modified July 1, 1993. Version 1. Checksum: 662C3DB5C8D44A94	FASTA	117	13,201
	10 20 30 40 50 60 MSSSTPFDPY ALSEHDEERP QNVQSKSRTA ELQAEIDDTV GIMRDNINKV AERGERLTSI 70 80 90 100 110 EDKADNLAVS AQGFKRGANR VRKAMWYKDL KMKMCLALVI IILLVVIIVP IAVHFSR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"SNC1, a yeast homolog of the synaptic vesicle-associated membrane protein/synaptobrevin gene family: genetic interactions with the RAS and CAP genes." Gerst J.E., Rodgers L., Riggs M., Wigler M. Proc. Natl. Acad. Sci. U.S.A. 89:4338-4342(1992) [PubMed: 1316605] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	Erratum Gerst J.E., Rodgers L., Riggs M., Wigler M. Proc. Natl. Acad. Sci. U.S.A. 89:7287-7287(1992)
[3]	"The nucleotide sequence of chromosome I from Saccharomyces cerevisiae." Bussey H., Kaback D.B., Zhong W.-W., Vo D.H., Clark M.W., Fortin N., Hall J., Ouellette B.F.F., Keng T., Barton A.B., Su Y., Davies C.J., Storms R.K. Proc. Natl. Acad. Sci. U.S.A. 92:3809-3813(1995) [PubMed: 7731988] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 204511 / S288c / AB972.
[4]	Saccharomyces Genome Database Submitted (DEC-2009) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: ATCC 204508 / S288c.
[5]	"A proteomics approach to understanding protein ubiquitination." Peng J., Schwartz D., Elias J.E., Thoreen C.C., Cheng D., Marsischky G., Roelofs J., Finley D., Gygi S.P. Nat. Biotechnol. 21:921-926(2003) [PubMed: 12872131] [Abstract] Cited for: UBIQUITINATION [LARGE SCALE ANALYSIS] AT LYS-63, MASS SPECTROMETRY. Strain: SUB592.
[6]	"Swf1-dependent palmitoylation of the SNARE Tlg1 prevents its ubiquitination and degradation." Valdez-Taubas J., Pelham H.R.B. EMBO J. 24:2524-2532(2005) [PubMed: 15973437] [Abstract] Cited for: PALMITOYLATION AT CYS-95.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

M91157 Genomic DNA. Translation: AAA35069.1.
U12980 Genomic DNA. Translation: AAC05002.1.
BK006935 Genomic DNA. Translation: DAA06958.1.

PIR

S31250.

RefSeq

NP_009372.1. NM_001178175.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3B5N	X-ray	1.60	A/E/I	27-86	[»]

ProteinModelPortal

P31109.

SMR

P31109. Positions 27-86.

DisProt

DP00113.

ModBase

Search...

Protein-protein interaction databases

DIP

DIP-2495N.

IntAct

P31109. 2 interactions.

STRING

P31109.

Proteomic databases

PeptideAtlas

P31109.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblFungi

YAL030W; YAL030W; YAL030W.

GeneID

851203.

KEGG

sce:YAL030W.

NMPDR

fig|4932.3.peg.52.

Organism-specific databases

CYGD

YAL030w.

SGD

S000000028. SNC1.

Phylogenomic databases

eggNOG

fuNOG10851.

GeneTree

EFGT00050000004849.

HOGENOM

HBG386095.

OMA

ESHAQTH.

OrthoDB

EOG4N606K.

Gene expression databases

ArrayExpress

P31109.

Genevestigator

P31109.

GermOnline

YAL030W. Saccharomyces cerevisiae.

Family and domain databases

InterPro

IPR001388. Synaptobrevin.
IPR016444. Synaptobrevin_met/fun.
[Graphical view]

KO

K08513.

Pfam

PF00957. Synaptobrevin. 1 hit.
[Graphical view]

PIRSF

PIRSF005409. Synaptobrevin_euk. 1 hit.

PRINTS

PR00219. SYNAPTOBREVN.

PROSITE

PS00417. SYNAPTOBREVIN. 1 hit.
PS50892. V_SNARE. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

968064.

Entry information

Entry name

SNC1_YEAST

Accession

Primary (citable) accession number: P31109
Secondary accession number(s): D6VPI8

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 1, 1993
Last modified:	December 14, 2011
This is version 103 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Fungal Protein Annotation Program