Reviewed, UniProtKB/Swiss-Prot P31105 (CHER_BACSU)

Names and origin

Protein names

Recommended name:
    Chemotaxis protein methyltransferase
    EC=2.1.1.80

Gene names

Name:	cheR
Ordered Locus Names:	BSU22720

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	256 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Predicted.

General annotation (Comments)

Function	Methylation of the membrane-bound methyl-accepting chemotaxis proteins (MCP) to form gamma-glutamyl methyl ester residues in MCP. CheR is responsible for the chemotactic adaptation to repellents.
Catalytic activity	S-adenosyl-L-methionine + protein L-glutamate = S-adenosyl-L-homocysteine + protein L-glutamate methyl ester.
Subunit structure	Monomer.
Sequence similarities	Contains 1 cheR-type methyltransferase domain.

Ontologies

Keywords
Biological process	Chemotaxis
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	protein-glutamate O-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 256	256	Chemotaxis protein methyltransferase		PRO_0000176030
Regions
	Domain	1 – 256	256	CheR-type methyltransferase
	Region	185 – 186	2	S-adenosyl-L-methionine binding By similarity
	Region	201 – 202	2	S-adenosyl-L-methionine binding By similarity
Sites
	Binding site	67	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine By similarity
	Binding site	73	1	S-adenosyl-L-methionine By similarity
	Binding site	107	1	S-adenosyl-L-methionine By similarity
	Binding site	130	1	S-adenosyl-L-methionine By similarity
Experimental info
	Sequence conflict	154 – 155	2	LS → VC in CAA52033. Ref.1
	Sequence conflict	156	1	V → M in AAA20858. Ref.2
	Sequence conflict	256	1	R → KID in AAA20858. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P31105-1 [UniParc]. Last modified August 15, 2003. Version 4. Checksum: A89786FF70A9F40D	FASTA	256	29,952
	10 20 30 40 50 60 MDTYSVFTTK WKQLTGVDLT LYKEAQMKRR LTSLYEKKGF QSFKDFAAAL EKDQALLNET 70 80 90 100 110 120 LDRMTINVSE FYRNYKRWEV LETAILPLIK TSRPLKIWSA ACSTGEEPYT LAMLLDQQKG 130 140 150 160 170 180 LPGYQILATD IDEKALEKAK KGVYQERSLQ EVPLSVKDRY FTQNANRSYE VKTEIKKNIT 190 200 210 220 230 240 FKKHNLLADR YEQDFDLIVC RNVFIYFTES AKEELYLKMA HSLKKNGVLF VGSTEQIFNP 250 EKFGLVPADT FFYQKR

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Chemotactic methyltransferase promotes adaptation to repellents in Bacillus subtilis." Kirsch M.L., Zuberi A.R., Henner D.J., Peters P.D., Yazdi M.A., Ordal G.W. J. Biol. Chem. 268:25350-25356(1993) [PubMed: 8244966] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 168 / OI1085.
[2]	"Sequence of Bacillus subtilis dbpA, mtr(A,B), gerC(1-3), ndk, cheR, aro(B,E,F,H), trp(A-F), hisH, and tyrA genes." Henner D.J. Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[3]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[4]	"Detecting and analyzing DNA sequencing errors: toward a higher quality of the Bacillus subtilis genome sequence." Medigue C., Rose M., Viari A., Danchin A. Genome Res. 9:1116-1127(1999) [PubMed: 10568751] [Abstract] Cited for: SEQUENCE REVISION.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X73681 Genomic DNA. Translation: CAA52033.1. M80245 Genomic DNA. Translation: AAA20858.1. AL009126 Genomic DNA. Translation: CAB14188.2.
PIR	A69599.
RefSeq	NP_390153.2.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939003.
GenomeReviews	Gene locus BSU22720 in contig AL009126_GR.
KEGG	bsu:BSU22720.
Organism-specific databases
SubtiList	BG10283. cheR. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P31105.
OMA	P31105. TLDRMTI.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2271-MON.
BRENDA	2.1.1.80. 150.
Family and domain databases
InterPro	IPR000780. CheR_MeTrfase. [Graphical view]
Pfam	PF01739. CheR. 1 hit. PF03705. CheR_N. 1 hit. [Graphical view]
PRINTS	PR00996. CHERMTFRASE.
SMART	SM00138. MeTrc. 1 hit. [Graphical view]
PROSITE	PS50123. CHER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHER_BACSU

Accession

Primary (citable) accession number: P31105
Secondary accession number(s): O32009

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	August 15, 2003
Last modified:	June 16, 2009
This is version 67 of the entry and version 4 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)