Reviewed, UniProtKB/Swiss-Prot P31104 (AROC_BACSU)

Names and origin

Protein names

Recommended name:
    Chorismate synthase
    EC=4.2.3.5
Alternative name(s):
    5-enolpyruvylshikimate-3-phosphate phospholyase
    Vegetative protein 216
      Short name=VEG216

Gene names

Name:	aroF
Ordered Locus Names:	BSU22710

Organism

Bacillus subtilis [Complete proteome] [HAMAP]

Taxonomic identifier

1423 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Bacillales › Bacillaceae › Bacillus

Protein attributes

Sequence length	390 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Catalytic activity	5-O-(1-carboxyvinyl)-3-phosphoshikimate = chorismate + phosphate. HAMAP MF_00300
Cofactor	Reduced flavin By similarity.
Pathway	Metabolic intermediate biosynthesis; chorismate biosynthesis; chorismate from D-erythrose 4-phosphate and phosphoenolpyruvate: step 7/7. HAMAP MF_00300
Subunit structure	Homotetramer By similarity.
Sequence similarities	Belongs to the chorismate synthase family.
Sequence caution	The sequence AAA20859.1 differs from that shown. Reason: Frameshift at position 355.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Aromatic amino acid biosynthesis
Molecular function	Lyase
Technical term	Complete proteome Direct protein sequencing
Gene Ontology (GO)
Biological process	aromatic amino acid family biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	chorismate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 390	390	Chorismate synthase HAMAP MF_00300		PRO_0000140553
Experimental info
	Sequence conflict	175	1	E → G in AAA20859. Ref.1
	Sequence conflict	362 – 363	2	NA → T in AAA20859. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P31104-1 [UniParc]. Last modified July 28, 2009. Version 3. Checksum: 9D529ABD63651885	FASTA	390	42,806
	10 20 30 40 50 60 MRYLTAGESH GPQLTTIIEG VPAGLYITEE DINFELARRQ KGHGRGRRMQ IEKDQAKIMS 70 80 90 100 110 120 GVRHARTLGS PIALVVENND WKHWTKIMGA APITEDEEKE MKRQISRPRP GHADLNGAIK 130 140 150 160 170 180 YNHRDMRNVL ERSSARETTV RVAAGAVAKK ILSELGIKVA GHVLQIGAVK AEKTEYTSIE 190 200 210 220 230 240 DLQRVTEESP VRCYDEEAGK KMMAAIDEAK ANGDSIGGIV EVIVEGMPVG VGSYVHYDRK 250 260 270 280 290 300 LDSKLAAAVL SINAFKGVEF GIGFEAAGRN GSEVHDEIIW DEEKGYTRAT NRLGGLEGGM 310 320 330 340 350 360 TTGMPIVVRG VMKPIPTLYK PLKSVDIETK EPFSASIERS DSCAVPAASV VAEAAVAWEI 370 380 390 ANAVVEQFGL DQIDRIRENV ENMRKLSREF

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Sequence of Bacillus subtilis dbpA, mtr(A,B), gerC(1-3), ndk, cheR, aro(B,E,F,H), trp(A-F), hisH, and tyrA genes." Henner D.J. Submitted (JAN-1992) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"The complete genome sequence of the Gram-positive bacterium Bacillus subtilis." Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V., Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R., Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S., Bruschi C.V. , Caldwell B., Capuano V., Carter N.M., Choi S.-K., Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F., Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D., Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M., Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P., Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K., Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S., Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y., Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G., Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J., Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C., Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S., Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B., Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S., Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M., Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y., Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J., Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A., Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M., Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S., Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E., Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K., Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E., Yoshikawa H., Danchin A. Nature 390:249-256(1997) [PubMed: 9384377] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 168.
[3]	"Detecting and analyzing DNA sequencing errors: toward a higher quality of the Bacillus subtilis genome sequence." Medigue C., Rose M., Viari A., Danchin A. Genome Res. 9:1116-1127(1999) [PubMed: 10568751] [Abstract] Cited for: SEQUENCE REVISION.
[4]	"From a consortium sequence to a unified sequence: the Bacillus subtilis 168 reference genome a decade later." Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A., Vallenet D., Wang T., Moszer I., Medigue C., Danchin A. Microbiology 155:1758-1775(2009) [PubMed: 19383706] [Abstract] Cited for: SEQUENCE REVISION TO 362-363.
[5]	"First steps from a two-dimensional protein index towards a response-regulation map for Bacillus subtilis." Antelmann H., Bernhardt J., Schmid R., Mach H., Voelker U., Hecker M. Electrophoresis 18:1451-1463(1997) [PubMed: 9298659] [Abstract] Cited for: PROTEIN SEQUENCE OF 1-10. Strain: 168 / IS58.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M80245 Genomic DNA. Translation: AAA20859.1. Frameshift. AL009126 Genomic DNA. Translation: CAB14187.3.
PIR	C69590.
RefSeq	NP_390152.3.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	939000.
GenomeReviews	Gene locus BSU22710 in contig AL009126_GR.
KEGG	bsu:BSU22710.
Organism-specific databases
SubtiList	BG10284. aroF. [Micado]
CMR	Search...
Phylogenomic databases
HOGENOM	P31104.
OMA	PPGLGSH.
Enzyme and pathway databases
BioCyc	BSUB224308:BSU2270-MON.
BRENDA	4.2.3.5. 150.
Family and domain databases
HAMAP	MF_00300. [Tree]
InterPro	IPR000453. Chorismate_synth. IPR020541. Chorismate_synthase_CS. [Graphical view]
PANTHER	PTHR21085. Chorismate_synth. 1 hit.
Pfam	PF01264. Chorismate_synt. 1 hit. [Graphical view]
PIRSF	PIRSF001456. Chorismate_synth. 1 hit.
ProDom	PD002941. Chorismate_synth. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00033. aroC. 1 hit.
PROSITE	PS00787. CHORISMATE_SYNTHASE_1. 1 hit. PS00788. CHORISMATE_SYNTHASE_2. 1 hit. PS00789. CHORISMATE_SYNTHASE_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AROC_BACSU

Accession

Primary (citable) accession number: P31104

Entry history

Integrated into UniProtKB/Swiss-Prot:	July 1, 1993
Last sequence update:	July 28, 2009
Last modified:	November 3, 2009
This is version 73 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)