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Protein

Acidic phospholipase A2 RV-7

Gene
N/A
Organism
Daboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Heterodimer: RV-4/RV-7 targets the presynaptic sites of the neuromuscular junction.1 Publication
Monomer: snake venom phospholipase A2 (PLA2) RV-7 that has low enzymatic activity and is not toxic. It inhibits the enzymatic activity of RV-4 in vitro but potentiates its lethal potency and neurotoxicity. It may facilitate the specific binding of RV-4 to its presynaptic binding sites, probably by acting as a chaperon, minimizing distraction and destruction of RV-4 en route to the site of action by reducing non-specific binding to muscle and other organs. PLA2 catalyzes the calcium-dependent hydrolysis of the 2-acyl groups in 3-sn-phosphoglycerides.1 Publication

Catalytic activityi

Phosphatidylcholine + H2O = 1-acylglycerophosphocholine + a carboxylate.PROSITE-ProRule annotation

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi43 – 431Calcium; via carbonyl oxygenBy similarity
Metal bindingi45 – 451Calcium; via carbonyl oxygenBy similarity
Metal bindingi47 – 471Calcium; via carbonyl oxygenBy similarity
Active sitei63 – 631By similarity
Metal bindingi64 – 641CalciumBy similarity
Active sitei105 – 1051By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Neurotoxin, Phospholipase A2 inhibitor, Presynaptic neurotoxin, Toxin

Keywords - Biological processi

Lipid degradation, Lipid metabolism

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Acidic phospholipase A2 RV-7 (EC:3.1.1.4)
Short name:
svPLA2
Alternative name(s):
F7
Phosphatidylcholine 2-acylhydrolase
Phospholipase A2 inhibitor
S4
Viperotoxin F
Viperotoxin non-toxic acidic component
OrganismiDaboia siamensis (Eastern Russel's viper) (Daboia russelii siamensis)
Taxonomic identifieri343250 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiLepidosauriaSquamataBifurcataUnidentataEpisquamataToxicoferaSerpentesColubroideaViperidaeViperinaeDaboia

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei1 – 16162 PublicationsAdd
BLAST
Chaini17 – 138122Acidic phospholipase A2 RV-7PRO_0000022978Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 1311 Publication
Disulfide bondi44 ↔ 601 Publication
Disulfide bondi59 ↔ 1111 Publication
Disulfide bondi65 ↔ 1381 Publication
Disulfide bondi66 ↔ 1041 Publication
Disulfide bondi73 ↔ 971 Publication
Disulfide bondi91 ↔ 1021 Publication

Keywords - PTMi

Disulfide bond

Expressioni

Tissue specificityi

Expressed by the venom gland.

Interactioni

Subunit structurei

Heterodimer of a weakly toxic basic protein having phospholipase A2 activity (RV-4) and a non-toxic acidic protein which inhibits its enzymatic activity but potentiates its lethal potency and neurotoxicity (RV-7).

Structurei

Secondary structure

1
138
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi18 – 2912Combined sources
Helixi33 – 353Combined sources
Helixi37 – 393Combined sources
Turni41 – 444Combined sources
Helixi55 – 6814Combined sources
Turni75 – 773Combined sources
Beta strandi82 – 854Combined sources
Beta strandi88 – 914Combined sources
Helixi96 – 11520Combined sources
Helixi116 – 1183Combined sources
Helixi121 – 1233Combined sources
Turni125 – 1295Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OQSX-ray1.90A/C/E/G17-138[»]
ProteinModelPortaliP31100.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP31100.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

HOVERGENiHBG008137.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P31100-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRTLWIVAVC LIGVEGNLFQ FGEMILEKTG KEVVHSYAIY GCYCGWGGQG
60 70 80 90 100
RAQDATDRCC FVHDCCYGTV NDCNPKTATY SYSFENGDIV CGDNDLCLRT
110 120 130
VCECDRAAAI CLGQNVNTYD KNYEYYSISH CTEESEQC
Length:138
Mass (Da):15,421
Last modified:July 1, 1993 - v1
Checksum:i6DC115BBA979827E
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti27 – 271E → Q AA sequence (PubMed:8835338).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68386 mRNA. Translation: CAA48457.1.
EU556499 mRNA. Translation: ACD43466.1.
EU556501 Genomic DNA. Translation: ACD43468.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X68386 mRNA. Translation: CAA48457.1.
EU556499 mRNA. Translation: ACD43466.1.
EU556501 Genomic DNA. Translation: ACD43468.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1OQSX-ray1.90A/C/E/G17-138[»]
ProteinModelPortaliP31100.
ModBaseiSearch...
MobiDBiSearch...

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

HOVERGENiHBG008137.

Miscellaneous databases

EvolutionaryTraceiP31100.

Family and domain databases

Gene3Di1.20.90.10. 1 hit.
InterProiIPR001211. PLipase_A2.
IPR033112. PLipase_A2_Asp_AS.
IPR016090. PLipase_A2_dom.
IPR033113. PLipase_A2_His_AS.
[Graphical view]
PANTHERiPTHR11716. PTHR11716. 1 hit.
PfamiPF00068. Phospholip_A2_1. 1 hit.
[Graphical view]
PRINTSiPR00389. PHPHLIPASEA2.
SMARTiSM00085. PA2c. 1 hit.
[Graphical view]
SUPFAMiSSF48619. SSF48619. 1 hit.
PROSITEiPS00119. PA2_ASP. 1 hit.
PS00118. PA2_HIS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Characterization and molecular cloning of neurotoxic phospholipases A2 from Taiwan viper (Vipera russelli formosensis)."
    Wang Y.-M., Lu P.-J., Ho C.-L., Tsai I.-H.
    Eur. J. Biochem. 209:635-641(1992) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PROTEIN SEQUENCE OF 17-66.
    Tissue: Venom and Venom gland.
  2. "Study of phospholipase A2 genes and their expressions in Thai Russell's viper venom glands."
    Sai-Ngam A., Phongtananant S., Nuchprayoon I.
    Submitted (MAR-2008) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Venom gland.
  3. "Two types of Russell's viper revealed by variation in phospholipases A2 from venom of the subspecies."
    Tsai I.-H., Lu P.-J., Su J.-C.
    Toxicon 34:99-109(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-66, FUNCTION, LETHAL DOSE.
    Tissue: Venom.
  4. "Snake venomics of the Siamese Russell's viper (Daboia russelli siamensis) -- relation to pharmacological activities."
    Risch M., Georgieva D., von Bergen M., Jehmlich N., Genov N., Arni R.K., Betzel C.
    J. Proteomics 72:256-269(2009) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 17-28 AND 107-121.
    Tissue: Venom.
  5. "Structure of the heterodimeric neurotoxic complex viperotoxin F (RV-4/RV-7) from the venom of Vipera russelli formosensis at 1.9 A resolution."
    Perbandt M., Tsai I.-H., Fuchs A., Banumathi S., Rajashankar K.R., Georgieva D., Kalkura N., Singh T.P., Genov N., Betzel C.
    Acta Crystallogr. D 59:1679-1687(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.9 ANGSTROMS) OF 17-138, DISULFIDE BONDS.
    Tissue: Venom.

Entry informationi

Entry nameiPA2A7_DABSI
AccessioniPrimary (citable) accession number: P31100
Secondary accession number(s): B2YHV2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 1, 1993
Last sequence update: July 1, 1993
Last modified: May 11, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
Annotation programAnimal Toxin Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.